scholarly journals Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions

Genome ◽  
2016 ◽  
Vol 59 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Hari D. Upadhyaya ◽  
Yi-Hong Wang ◽  
Dintyala V.S.S.R. Sastry ◽  
Sangam L. Dwivedi ◽  
P.V. Vara Prasad ◽  
...  

Sorghum is one of the world’s most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum’s growing season and increases yield in temperate regions. However, sorghum’s sensitivity to low soil temperatures adversely impacts seed germination. In this study, we evaluated the 242 accessions of the ICRISAT sorghum mini core collection for seed germination and seedling vigor at 12 °C as a measure of cold tolerance. Genome-wide association analysis was performed with approximately 162 177 single nucleotide polymorphism markers. Only one marker locus (Locus 7-2) was significantly associated with low-temperature germination and none with vigor. The linkage of Locus 7-2 to low-temperature germination was supported by four lines of evidence: strong association in three independent experiments, co-localization with previously mapped cold tolerance quantitative trait loci (QTL) in sorghum, a candidate gene that increases cold tolerance and germination rate when its wheat homolog is overexpressed in tobacco, and its syntenic region in rice co-localized with two cold tolerance QTL in rice. This locus may be useful in developing tools for molecular breeding of sorghums with improved low-temperature germinability.

2019 ◽  
Vol 20 (15) ◽  
pp. 3796 ◽  
Author(s):  
Xin Wang ◽  
Chao Yu ◽  
Yi Liu ◽  
Lu Yang ◽  
Yang Li ◽  
...  

Low temperature is an environmental stress factor that is always been applied in research on improving crop growth, productivity, and quality of crops. Polyunsaturated fatty acids (PUFAs) play an important role in cold tolerance, so its genetic manipulation of the PUFA contents in crops has led to the modification of cold sensitivity. In this study, we over-expressed an ω-3 fatty acid desaturase from Glycine max (GmFAD3A) drove by a maize ubiquitin promoter in rice. Compared to the wild type (ZH11), ectopic expression of GmFAD3A increased the contents of lipids and total PUFAs. Seed germination rates in GmFAD3A transgenic rice were enhanced under low temperature (15 °C). Moreover, cold tolerance and survival ratio were significantly improved in GmFAD3A transgenic seedlings. Malondialdehyde (MDA) content in GmFAD3A transgenic rice was lower than that in WT under cold stress, while proline content obviously increased. Meanwhile, the activities of superoxide dismutase (SOD), hydroperoxidase (CAT), and peroxidase (POD) increased substantially in GmFAD3A transgenic rice after 4 h of cold treatment. Taken together, our results suggest that GmFAD3A can enhances cold tolerance and the seed germination rate at a low temperature in rice through the accumulation of proline content, the synergistic increase of the antioxidant enzymes activity, which finally ameliorated the oxidative damage.


2021 ◽  
Author(s):  
Caijing Li ◽  
Jindong Liu ◽  
Jianxin Bian ◽  
Tao Jin ◽  
Baoli Zou ◽  
...  

Abstract Background: Rice is a crop that is very sensitive to low temperature, and its morphological development and production are greatly affected by low temperature. Therefore, understanding the genetic basis of cold tolerance in rice is of great significance for mining favorable genes and cultivating excellent rice varieties. However, there were limited studies focusing on cold tolerance at the bud burst stage, therefore, considerable attention should be paid to the genetic basis of cold tolerance at the bud burst stage (CTBB).Results: In this study, a natural population consisting of 211 rice landraces collected from 15 provinces of China and other countries were firstly used to evaluate the cold tolerance at the bud burst stage. Population structure analysis showed that this population divided into three groups and was rich in genetic diversity. Our evaluation results confered that the japonica rice was more tolerance to cold at the bud burst stage than indica rice. Genome-wide association study (GWAS) were performed through the phenotypic data of 211 rice landraces and 36,727 SNPs dataset under a mixed linear model, and 12 QTLs (P < 0.0001) were identified according to the seedling survival rate (SSR) treated at 4 ℃, in which there are five QTLs (qSSR2-2, qSSR3-1, qSSR3-2, qSSR3-3 and qSSR9) which were co-located with previous studies, and seven QTLs (qSSR2-1, qSSR3-4, qSSR3-5, qSSR3-6, qSSR3-7, qSSR4 and qSSR7) which were reported for the first time. Among these QTLs, qSSR9, harboring the highest-peak SNP, explained biggest phenotypic variation. Through bioinformatics analysis, five genes (LOC_Os09g12440, LOC_Os09g12470, LOC_Os09g12520, LOC_Os09g12580 and LOC_Os09g12720) were nominated as candidates for qSSR9. Conclusion: This natural population consisting of 211 rice landraces with high density SNPs will serve as a better choice for identifying rice QTLs/genes in future, and the detected QTLs associated with cold tolerance in rice bud burst stage will be conducive to further mining favorable genes and breeding of rice varieties under cold stress.


2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2375
Author(s):  
Edlânia Maria de Souza ◽  
Edna Ursulino Alves ◽  
Maria Lúcia Maurício da Silva ◽  
Luciana Rodrigues de Araújo ◽  
Maria Das Mercês Serafim dos Santos Neta

Cajanus cajan (L.) Millspaugh, a member of the Fabaceae, is commonly known as feijão-guandu. It was introduced into Brazil because it readily adapts to local climate conditions and tolerates a wide range of water and mineral nutrient levels. The objective of this study was to determine the substrate temperatures and water volumes that are conducive to feijão-guandu seed germination and seedling vigor. The experiment was conducted at the Laboratory of Seed Analysis of the Centre of Agricultural Sciences, Federal University of Paraíba, Areia, PB, Brazil. Seed germination was tested using paper towel rolls moistened with volumes of water equivalent to 2, 2.5, 3, 3.5, and 4 times the dry mass of the substrate. Variable temperatures in the range of 20 °C to 30 °C and constant temperatures of 25 °C, 30 °C, and 35 °C were used. The experimental design was completely randomized. The variables analyzed were water content, percent germination, first germination count, germination rate index, and seedling root and shoot lengths and dry weights. Increasing the volume of water used to moisten the substrate hindered feijão-guandu germination at all temperatures. It was determined that moistening the substrate with water volumes equivalent to 2.8 and 3.5 times the substrate dry weight at 30 °C and 35 °C, respectively, were the ideal conditions for evaluating feijão-guandu seed germination and seedling vigor.


Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 40
Author(s):  
Li ◽  
Eyre ◽  
Broad ◽  
Rodriguez

Water and heat stressors during flowering are predominant limitations of dryland sorghum yields across Australia’s north-eastern cropping zone. Crops sown early could flower before seasonal heatwaves, but the seed must uniformly germination and emerge at soil temperatures between approximately 10 to 15 °C. Furthermore, chemical coatings applied to commercial hybrid seed lots effectively protected the developing crop from pest, disease and herbicide damage but the combined influence of low temperatures and seed coatings on germination of hybrid sorghum seed batches is unknown. In this experiment, germination dynamics were modelled for 10 commercial sorghum hybrid-seed lots (with or without seed coating of a.i. thiamethoxam, oxabetrinil and thiram) incubated at continuous temperatures ranging from 9.4 to 46.1 °C. Results also show commercial seed treatments negatively affect final germination proportion especially at sub- and supra-optimal temperatures. Some hybrid-seed lots in current experiment were more sensitive to seed coatings at either sub- or supra-optimal temperatures regardless of seed germination capability (i.e., germination proportion at 25 °C). Seeds took 5 days to reach 50% germination (t50) at 9.4 °C but germinated within 24 h at constant temperatures between 20 and 40 °C. The spread of germination (time between 10 and 90% germination) increased from 0.5 days at 30 °C to 3.8 days at 9.4 °C. Therefore, some hybrid-seedlots are suited to early sowing because they can maintain high germination proportion (>90%) even at soil temperatures (9.4 °C) as long as the seedbed remains moist for a least 12 days. Research continues identify the basis of cold tolerance in some-hybrid seedlots.


2019 ◽  
Author(s):  
Chathurika Wijewardana ◽  
K. Raja Reddy ◽  
L. Jason Krutz ◽  
Wei Gao ◽  
Nacer Bellaloui

AbstractEffects of environmental stressors on the parent may be transmitted to the F1 generation of plants that support global food, oil, and energy production for humans and animals. This study was conducted to determine if the effects of drought stress on parental soybean plants are transmitted to the F1 generation. The germination and seedling vigor of F1 soybean whose maternal parents, Asgrow AG5332 and Progeny P5333RY, were exposed to soil moisture stress, that is, 100, 80, 60, 40, and 20% replacement of evapotranspiration (ET) during reproductive growth, were evaluated under controlled conditions. Pooled over cultivars, effects of soil moisture stress on the parents caused a reduction in the seed germination rate, maximum seed germination, and overall seedling performance in the F1 generation. The effect of soil moisture stress on the parent induced an irreversible change in the seed quality in the F1 generation and the effects on seed quality in the F1 generation were exasperated when exposed to increasing levels of drought stress. Results indicate that seed weight and storage reserve are key factors influencing germination traits and seedling growth. Our data confirm that the effects of drought stress on soybean are transferable, causing reduced germination, seedling vigor, and seed quality in the F1 generation.


Author(s):  
Z.Z. Petkevich

Goal. To assess rice cultivars for cold tolerance; to select the most valuable in terms of economic characteristics sources for their further use in breeding; to create a trait collection on their basis.Result and Discussion. The article presents the results of studying a rice collection. The collection material is represented by 51 accessions of different eco-geographical origin (European, Eastern, Mid-Asian, Latin American). The accessions belong to two subspecies: japonica and indica. Most of the accessions (22) are from Ukraine and account for 43.1%. Temperature is one of the most important environmental factors affecting the crop establishment and development. In Ukraine, rice is cultivated in the most northern zone of rice growing and, therefore, it is liable to negative effects of cold weather during the seed germination, sprouting, anthesis and grain ripening. Consequently, it is essential for breeders to create cultivars that will be tolerant to low temperatures during the sprouting period, with high field germinability and increased germinative power. Seed germination is particularly affected by temperature. Fifty one rice cultivars were investigated for the seedling development, seed germination rate and seedling growth intensity at 14°C; possible states of embryonic roots were estimated; lengths of these stages were determined. Selection of material with a set of parameters allowed forming a trait collection with increased levels of resistance to abiotic environmental factors. In breeding, it is very important to identify sources of valuable traits and to assess extent of their influence on the plant performance. The research distinguished rice accessions for breeding by levels of cold tolerance, performance and its components. We selected six donors of several valuable traits, the use of which will enhance the breeding efficiency (Kuban 3, Vikont, UkrNDS 8839, Ontario, VNIIR 546, Kaz ER – 6). Thus, the comprehensive assessment of the accessions allowed us not only to replenish the rice gene pool, but also, basing on this research, to choose starting material to involve it in further breeding for creating new cold-tolerant varieties. While creating such cultivars, we revealed morphological traits of rice, which are used as test parameters characterizing the stress tolerance of accessions.Conclusions. The study resulted in formation of the trait collection representing the genetic diversity of cultivars and breeding lines. Each trait and gradation has corresponding standards. Cultivars-standards reflect more specific and stable expression of a trait. The collection was created by 4 basic traits and 3 gradations and contains accessions from 9 countries (Ukraine, Russia, Kazakhstan, Uzbekistan, Kyrgyzstan, USA, Bulgaria, China, and Hungary). The standard accessions selected can serve as sources of economically valuable features to create starting material for breeding.


Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 620
Author(s):  
Cristina Patanè ◽  
Salvatore L. Cosentino ◽  
Valeria Cavallaro ◽  
Alessandro Saita

Within the project “BIOSEA” funded by the Italian Ministry of Agriculture and Forestry, a preliminary laboratory test was conducted to assess the variability for cold tolerance during germination in 30 cultivars of biomass sorghum, among fiber and sweet types. Seed germination (%) and mean germination time (MGT) were examined at seven constant temperatures (from 8 °C to 35 °C) and base temperature (Tb) and thermal time (θT) for 50% germination were calculated. A wide genetic diversity in the germination response of sorghum was ascertained at 8 °C (CV 45%) and 10 °C (CV 25.4%). At 8 °C, in cultivars of ‘Padana 4’, ‘PR811F’, ‘PSE24213’, ‘PR849’ and ‘Zerberus’, seed germination exceeded 80%. Seeds of ‘Zerberus’ were also the fastest, requiring less than 13 days for final germination at this low temperature. Great differences were found in Tb and θT among cultivars. Tb varied between 7.44 °C (‘PR811F’) and 13.48 °C (‘Nectar’). Thermal time (θT) was, on average, 24.09 °Cd−1, and ranged between 16.62 (‘Nectar’) and 33.42 °Cd−1 (‘PSE24213’). The best combination of the two germination parameters (i.e., low Tb and θT) corresponded to ‘Zerberus’, ‘Sucrosorgo 506’, ‘Jumbo’ and ‘PR811F’. Accordingly, these cultivars are more tolerant to cold stress during germination and, thus, more adapt to early spring sowings in Mediterranean areas (March-April). Cultivars ‘PR811F’ (fiber type) and ‘Sucrosorgo 506’ (sweet type) also combine high cold tolerance with good productivity in terms of final dry biomass, as assessed in open-field conditions (late spring sowing). The genetic variation in the germination response to a low temperature is useful for the identification of genotypes of sorghum suitable to early sowings in semi-arid areas. Selection within existing cultivars for cold tolerance during germination may also contribute to the expansion of biomass sorghum into cooler cultivation areas, such as those of Northern Europe, which are less suitable to this warm season crop.


1993 ◽  
Vol 73 (3) ◽  
pp. 697-702 ◽  
Author(s):  
P. Hucl

Seed germination of diverse common bean (Phaseolus vulgaris L.) genotypes was evaluated under controlled suboptimal conditions. Initially, 16 genotypes were tested at 12 and 16 °C. A subset of genotypes was subsequently germinated in distilled water or in polyethylene glycol solutions with water potentials (WP) of either −0.4 or −0.8 MPa at temperatures of 22, 26 or 34 °C. Genotypes did not respond consistently to low temperature stress, as indicated by significant (P < 0.05) crossovers in genotype ranking for percent germination from one temperature (12 °C) to the next (16 °C). Genotypes differed in median germination time (GT50) and estimated maximum germination rate. Genotypes with thinner seeds germinated better than wide-seeded genotypes under low-temperature conditions. Seed germination was reduced by 10 percentage units at a WP of −0.4 MPa and was virtually nil at a WP of −0.8 MPa. GT50 was reduced by 30% at 26 versus 22 °C but delayed by 80% when the WP was lowered from 0 to −0.4 MPa. Similarly, maximum germination rate increased with temperature but decreased with WP. Based on the germplasm evaluated in this study, common bean genotypes differ in seed germination response to temperature and moisture stress. Key words: Common bean, Phaseolus vulgaris L., germination, temperature, water potential


2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2375
Author(s):  
Edlânia Maria de Souza ◽  
Edna Ursulino Alves ◽  
Maria Lúcia Maurício da Silva ◽  
Luciana Rodrigues de Araújo ◽  
Maria Das Mercês Serafim dos Santos Neta

Cajanus cajan (L.) Millspaugh, a member of the Fabaceae, is commonly known as feijão-guandu. It was introduced into Brazil because it readily adapts to local climate conditions and tolerates a wide range of water and mineral nutrient levels. The objective of this study was to determine the substrate temperatures and water volumes that are conducive to feijão-guandu seed germination and seedling vigor. The experiment was conducted at the Laboratory of Seed Analysis of the Centre of Agricultural Sciences, Federal University of Paraíba, Areia, PB, Brazil. Seed germination was tested using paper towel rolls moistened with volumes of water equivalent to 2, 2.5, 3, 3.5, and 4 times the dry mass of the substrate. Variable temperatures in the range of 20 °C to 30 °C and constant temperatures of 25 °C, 30 °C, and 35 °C were used. The experimental design was completely randomized. The variables analyzed were water content, percent germination, first germination count, germination rate index, and seedling root and shoot lengths and dry weights. Increasing the volume of water used to moisten the substrate hindered feijão-guandu germination at all temperatures. It was determined that moistening the substrate with water volumes equivalent to 2.8 and 3.5 times the substrate dry weight at 30 °C and 35 °C, respectively, were the ideal conditions for evaluating feijão-guandu seed germination and seedling vigor.


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