Oxidative Stress After Three Different Intensities of Running

2005 ◽  
Vol 30 (6) ◽  
pp. 723-734 ◽  
Author(s):  
Cláudia Dornelles Schneider ◽  
Jaqueline Barp ◽  
Jerri Luiz Ribeiro ◽  
Adriane Belló-Klein ◽  
Alvaro Reischak Oliveira
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Ventilatory Threshold ◽  
Enzymatic Antioxidants ◽  
Trained Subjects ◽  
Before And After ◽  
Total Antioxidant ◽  
Key Words Free Radicals ◽  
Increase Lipid Peroxidation ◽  
Uptake Data

In 8 trained subjects (T) and 9 untrained subjects (UT), lipid peroxidation (LPO), total antioxidant capacity (TRAP), superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the blood before and after three different intensities of exercise on the treadmill, determined from ventilatory threshold and maximal oxygen uptake data, obtained from a maximal aerobic power test. In plasma, LPO decreased from 3589 ± 193 to 3274 ± 223 cps•mg Hb−1(p <  0.05), and TRAP increased from 304 ± 45 to 384 ± 57 µmol•L−1trolox (p <  0.05) after high intensity exercise in T. GPx activity increased in the T group as compared to the UT group, after exercise in moderate (25.90 ± 3.79 to 15.05 ± 3.23 nM•min−1•mg protein−1) and high (21.75 ± 4.91 to 12.1 ± 2.46 nM•min−1•mg protein−1) intensities (p <  0.05). Superoxide dismutase activity increased after exercise at low (8.35 ± 0.85 to 9.23 ± 1.03 U SOD•mg protein−1) and moderate (8.89 ± 0.98 to 10.44 ± 0.86 U SOD•mg protein−1) intensity in UT (p <  0.05). There were no changes in catalase activity. These findings indicate that exercise in this model did not increase lipid peroxidation, probably because of the alterations in TRAP and enzymatic antioxidants. Key words: free radicals, lipid peroxidation, erythrocytes, antioxidant enzymes, exercise

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

scholarly journals Impact of nutritional rehabilitation on enzymatic antioxidant levels in protein energy malnutrition

2002 ◽  
Vol 8 (2-3) ◽  
pp. 290-297
Author(s):  
S. Y. Shaaban
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Protein Energy Malnutrition ◽  
Enzymatic Antioxidants ◽  
Nutritional Rehabilitation ◽  
Protein Energy ◽  
Before And After ◽  
Nutrition Rehabilitation ◽  
Laboratory Investigations

To assess the role of enzymatic antioxidants in the pathogenesis of protein energy malnutrition [PEM] and the effect of nutritional rehabilitation, we studied 30 infants with PEM [mean age 10.63 +/- 4.39 months: 10 marasmic; 8 with kwashiorkor; 12 with marasmic kwashiorkor] and 15 controls. All underwent clinical examination and laboratory investigations, including superoxide dismutase [SOD] and glutathione peroxidase [GPx] estimation before and after nutrition rehabilitation. SOD and GPx were significantly lower in all malnourished infants compared to controls, and significantly increased after nutritional rehabilitation. These significant correlations suggest that antioxidants could be introduced during PEM nutritional rehabilitation to decrease morbidity and mortality.

scholarly journals Redox status in the blood of ewes in the perinatal period and during lactation

2015 ◽  
Vol 59 (4) ◽  
pp. 557-562 ◽  
Author(s):  
Katarzyna Ognik ◽  
Krzysztof Patkowski ◽  
Tomasz Gruszecki ◽  
Krzysztof Kostro
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Uric Acid ◽  
Lipid Peroxides ◽  
Perinatal Period ◽  
Redox Status ◽  
Blood Indices ◽  
Before And After ◽  
Total Antioxidant ◽  
Stress Oxidative

Abstract The aim of the study was to determine the effect of the perinatal period on redox status indicators in the blood of ewes before and after lambing and during lactation. The study was performed on 12 ewes of the synthetic SCP line. Blood for testing of redox parameters was collected seven times: before pregnancy, 1.5 months and 24 h before lambing, 2 and 24 h after lambing, and in the fourth and eighth weeks of lactation. The following blood indices were determined by spectrophotometry: lipid peroxides, malondialdehyde, superoxide dismutase, catalase, plasma total antioxidant capacity, uric acid, urea, bilirubin, and creatinine. The tests showed that during the perinatal period reactions are generated which lead to oxidative stress. Oxidative stress in pregnant ewes was found to increase during the period before lambing and may persist even up to weeks 4-8 of lactation.

scholarly journals Serum biochemistry and oxidative stress indicators in West African dwarf goats under semi intensive management system

2020 ◽  
Vol 44 (4) ◽  
pp. 110-117
Author(s):  
U. G. D. Ihejirika ◽  
O. O. Adenekan ◽  
O. A. Jimoh ◽  
A. A. Jack ◽  
U. C. Uwaeziozi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Superoxide Dismutase ◽  
High Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Biochemistry ◽  
Total Antioxidant Activity ◽  
Intensive Management ◽  
Total Antioxidant

 This study aims to evaluate variations in serum biochemistry and oxidative status between male and female WestAfrican dwarfgoats under semi intensive management system. Twenty, each of bucks and does (12 months old) were randomly selected from a herd purchased from Oyo livestock market, in Oyo State. The average body weights of the goats were12.08 ± 3.26 and 12.15 ± 1.51kg does and bucks, respectively. Blood samples were collected from fastened goats and assayed for serum biochemicals, lipid peroxidation,  total antioxidant activity, catalase and superoxide dismutase using standard procedures. Data obtained were subjected to T-test. The result showed serum glucose, albumin,  cholesterol, total protein, globulin, triglyceride, creatinine, alanine amino transferase(ALT),aspartate amino transferase(AST) and urea were not significantly (P>0.05) different among the sexes. Serum high density lipoprotein was significantly (P<0.05) higher in does (13.96 mmol/l) than in bucks (5.91mmol/l) and alkaline phosphatase was significantly (P<0.05) higher in bucks (2580.55 IU/L) than in does (1594.58 IU/L). Serum lipid peroxidation, total antioxidant activity and superoxide dismutase were statistically similar (P>0.05) in both sexes. Serum catalase was significantly (P<0.05) higher in bucks than in does. From this study, it can be deduced that serum biochemical indices in does and bucks are similar except for alkaline phosphatase and high density lipoprotein. Bucks recorded higher catalase than does.It could be concluded that during physiological and environmental stress conditions, antioxidant supplementation should be adopted to boost the antioxidant capacity of the does.

scholarly journals The effect of selenium supplementation in organic and in organic form on oxidant balance in rat heart and femoral muscle

2013 ◽  
Vol 26 (3) ◽  
pp. 287-291
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Chain Structure ◽  
Ring Structure ◽  
Selenium Supplementation ◽  
Enzymatic Antioxidants ◽  
Organic Selenium ◽  
Total Antioxidant ◽  
Femoral Muscle

Total antioxidant status (TAS), activity of antioxidant enzymes - superoxide dismutase (SOD) and glutathione peroxidase (GPx), concentrations of non-enzymatic antioxidants - ascorbic acid (AA) and reduced glutathione (GSH) as well as concentration of lipid peroxidation marker - malonyldialdehyde (MDA) were determined in heart and femoral muscle of rats receiving different selecompounds (inorganic selenite and organic selenosemicarbazide of chain structure and selenazoline of ring structure). Chain selenosemicarbazide markedly decreased TAS values vs. control without Se-supplementation in heart. GPx was significantly depressed vs. control in heart of animals receiving organic selenium. Ring selenazoline decreased heart AA, whereas selenosemicarbazide increased heart GSH. Inorganic selenite diminished femoral muscle GSH. Selenium supplementation distinctly inhibited process of lipid peroxidation - MDA was decreased in Se-given animals, particularly in heart. As organic ring selenocompound depressed heart MDA to the highest degree, did not impair total antioxidant status and caused no disturbance of antioxidant barrier in femoral muscle, it could be suggested that further research may reveal possibilities of its application as a Se-supplement.

scholarly journals Assessment of oxidative stress indicators of West African Dwarf sheep under semi intensive management system

2021 ◽  
Vol 43 (2) ◽  
pp. 78-83
Author(s):  
A. A Jack ◽  
O. A. Jimoh ◽  
U. G. Ihejirika ◽  
U. C. Uwaeziozi ◽  
O. O. Adenekan
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Serum Biochemistry ◽  
West African ◽  
Total Antioxidant Activity ◽  
West African Dwarf ◽  
Total Antioxidant ◽  
Serum Biochemical

Oxidative stress results from increased production of free radicals and reactive oxygen species, and a decrease in antioxidant defense. This study was conducted to assess sexual dimorphism in serum biochemistry and oxidative status of West African dwarf lambs. Sixteen each of rams and ewes 8months old were randomly selected from a herd purchase from livestock market, Oyo. The average body weights of the lambs were 12.08±3.26 and 12.76kg ewes and rams, respectively. Fastened blood samples were collected from the sheep and assayed for serum biochemical, lipid peroxidation, total antioxidant activity, catalase and superoxide dismutase using standard procedures, data obtained were subjected to T-test. The result indicated that both sex had similar serum biochemical indices except total protein. Ewes had significantly higher serum protein than ram. Rams had apparently higher total antioxidant activity and significantly higher serum catalase activity than ewes, while serum lipid peroxidation and superoxide dismutase were statistically similar in both sexes. This indicates that rams have better antioxidant activity than ewes. During oxidative stress prone conditions and environment, measures to boost antioxidant activity of ewes should be emphasized.

scholarly journals Anaerobic Exercise Affects the Saliva Antioxidant/Oxidant Balance in High-Performance Pentathlon Athletes

2016 ◽  
Vol 17 (1) ◽  
Author(s):  
Marcelo de Lima Sant’Anna ◽  
Gustavo Casimiro-Lopes ◽  
Gabriel Boaventura ◽  
Sergio Tadeu Farinha Marques ◽  
Martha Meriwether Sorenson ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Uric Acid ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
High Performance ◽  
Anaerobic Exercise ◽  
Uric Acid Concentration ◽  
Before And After ◽  
Total Antioxidant ◽  
Anaerobic Test

AbstractPurpose. Investigate free radical production and antioxidant buffering in military pentathletes’ saliva after their performance of a standardized, running-based anaerobic sprint test (RAST). Methods. Seven members of the Brazilian Navy pentathlon team were recruited to perform a running-based anaerobic test (~90 sec). The participants provided samples of saliva before and after the test that were analyzed for biomarkers of oxidative stress such as lipid peroxidation, total antioxidant capacity and the quantity of two specific antioxidants, glutathione and uric acid. Results. The lipid peroxidation increased ~2 fold after RAST, despite an increase in total antioxidant capacity (46%). The concentration of reduced glutathione did not change, while the uric acid concentration increased by 65%. Conclusions. The evaluation in saliva following a sprint test that lasted no more than 90 sec was sensitive enough to reveal changes in redox state.

scholarly journals Assessment of Antioxidant Status and Lipid Peroxidation in Pre-Eclampsia

2019 ◽  
pp. 1-9
Author(s):  
Patrick O. Manafa ◽  
Charles C. Onyenekwe ◽  
Anselem C. Igwe ◽  
Nancy A. Mbachu ◽  
George O. Chukwuma ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Positive Correlation ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Blood Pressures

Aim: To assess the role of antioxidants and lipid peroxidation in the pathogenesis of pre-eclampsia. Study Design: This is a case-control study designed to evaluate the levels of malondialdehyde and total antioxidant capacity with glutathione peroxidase and superoxide dismutase activities in pre-eclamptic. One hundred (100) participants were randomly selected to include fifty pre-eclamptic (test), twenty-five (25) healthy pregnant normotensive women (control A) and twenty-five healthy non-pregnant normotensive women (control B). Place and Duration of Study: This study was carried out at the Holy Rosary Hospital waterside, Onitsha, Anambra state. Nigeria. The study lasted for 13 months. Methodology: One hundred women (50 pre-eclamptic, 25 pregnant normotensives and 25 non-pregnant normotensives) aged 25-40 years were recruited for this study. Blood samples were collected from the participants for the estimation of total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities using spectrophotometric methods. Blood pressures were measured using accosson sphygmomanometer. Data were analysed using analysis of variance and Pearson’s correlation coefficient at p < 0.05. Results: The TAC was significantly higher in the pregnant normotensives (p< 0.05) compared with the pre-eclamptic and non-pregnant normotensives. There was no significant difference in the MDA in pre-eclamptic compared with pregnant normotensives and non-pregnant normotensives (p>0.05). A significant difference was observed in the SOD and GPx activities in the pregnant normotensive and non-pregnant normotensives compared with pre-eclamptic. A positive correlation was observed between the blood pressures (systolic and diastolic) and the activities of TAC, SOD and GPx in pre-eclamptic. Furthermore, a positive correlation was observed between MDA levels and SBP (r = 0.019) while a negative correlation (r = -0.225) existed between MDA and DBP. Conclusion: Diminished ability of antioxidants to scavenge free radicals may affect the onset of pre-eclampsia and therefore possible prognostic tool in its management.

Antioxidant and developmental capacity of retinol on the in vitro culture of rabbit embryos

Zygote ◽  
2018 ◽  
Vol 26 (4) ◽  
pp. 326-332 ◽  
Author(s):  
Ahmed M. Elomda ◽  
Mohamed F. Saad ◽  
Ayman M. Saeed ◽  
Ashraf Elsayed ◽  
Ahmed O. Abass ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Blastocyst Stage ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Vertebrate Development ◽  
Junction Protein ◽  
Total Antioxidant ◽  
Developmental Capacity ◽  
Rabbit Embryos

SummaryOxidative stress is a major cause of defective embryo development during in vitro culture. Retinoids are recognized as non-enzymatic antioxidants and may have an important role in the regulation of cell differentiation and vertebrate development. However, there are not enough reports discussing the antioxidant and developmental capacity of retinoids, including retinol (RT), on the in vitro development of embryos recovered from livestock animals, particularly in rabbit species. Therefore, morula embryos obtained from nulliparous Red Baladi rabbit does were cultured for 48 h in TCM199 medium in the absence of RT (control group) or in the presence of RT at concentrations of 10, 100 and 1000 nM. The developmental capacity to the hatched blastocyst stage, the antioxidant biomarker assay and the expression of several selected genes were analyzed in each RT group. The data show that RT significantly (P<0.001) promoted the embryo hatchability rate at the concentration of 1000 nM to 69.44% versus 29.71% for the control. The activity of malondialdehyde (MDA) level was significantly (P<0.05) lower in the RT groups than in the control group, while the total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities were significantly (P<0.05) higher following treatment with RT. Furthermore, RT treatment considerably upregulated the relative expression of gap junction protein alpha 1 (GJA1), POU class 5 homeobox 1 (POU5F1) and superoxide dismutase 1 (SOD1) genes compared with the control group. The current study highlights the potential effects of RT as antioxidant in the culture medium on the in vitro development of rabbit embryos.

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