EFFECT OF THE ISOMETRIC -ISOTONIC EXERCISE ON LIGAMENTS RUPTURE INJURY OF KNEE AND REHABILITATION FOR ATHLETIC

Introduction.Rehabilitation is a program of treatment with some sport exercises targeting the lower body .The aim:of the study is to develop the work of the knee joint after the ligament rupture injury and to rehabilitate itby preparation of the (PTP) proposed training program.Material and Method:The study included 4 injured athletes who were selected from a total of 10 injured.The tools used to measure the angle of flexion and extension of the knee joint before and after rehabilitation are tape measure , anthropometric measurements and squat equipment. Statistical analyses:which used included MEAN, STD, MAX, MIN, MEDIAN to explain the results. Anthropometricis used to measure the (TCUIL) Thigh circumference of upper injured limb and (CBIL) Circumference of bottom injured, Flexion and extension angle of the knee joint and isometric -isotonic exercises with Pre-test –post-test for 4 injures athletes. Results :using the intensity of 30-35% until it reached 95-100% after the end of the training program with 9 -10Kg weights in the first week until 30-35 Kg on squat equipment at the end of the fourth week and it was cured and Anthropometrics of Pre-test was (TCUIL) was 49 and Post-test was 50 cm with increase1cmand the Pre-test of (CBIL) was 34.5 and Post-test 34.7 cm with increase(0.2mm).Conclusions:: The (PTP) developed muscle strength of the femoral muscle groups working on the knee joint after used isometric isotonic exercise

Novel protocol combining physical and nutrition therapies, Intensive Goal-directed REhabilitation with Electrical muscle stimulation and Nutrition (IGREEN) care bundle

Background Although the combination of rehabilitation and nutrition may be important for the prevention of intensive care unit (ICU)-acquired weakness, a protocolized intervention of this combination has not yet been reported. We herein developed an original combined protocol and evaluated its efficacy. Methods In this single-center historical control study, we enrolled adult patients admitted to the ICU. Patients in the control group received standard care, while those in the intervention group received the protocol-based intervention. The ICU mobility scale was used to set goals for early mobilization and a neuromuscular electrical stimulation was employed when patients were unable to stand. The nutritional status was assessed for nutritional therapy, and target calorie delivery was set at 20 or 30 kcal/kg/day and target protein delivery at 1.8 g/kg/day in the intervention group. The primary endpoint was a decrease in femoral muscle volume in 10 days assessed by computed tomography. Results Forty-five patients in the control group and 56 in the intervention group were included in the analysis. Femoral muscle volume loss was significantly lower in the intervention group (11.6 vs 14.5%, p = 0.03). The absolute risk difference was 2.9% (95% CI 0.1–5.6%). Early mobilization to a sitting position by day 10 was achieved earlier (p = 0.03), and mean calorie delivery (20.1 vs. 16.8 kcal/kg/day, p = 0.01) and mean protein delivery (1.4 vs. 0.8 g/kg/day, p < 0.01) were higher in the intervention group. Conclusion The protocolized intervention, combining early mobilization and high-protein nutrition, contributed to the achievement of treatment goals and prevention of femoral muscle volume loss. Trial registration number The present study is registered at the University Hospital Medical Information Network-clinical trials registry (UMIN000040290, Registration date: May 7, 2020).

IMPACT OF GREEN FORAGES IN SPRING, IN THE STEPPE REGIONS OF TIARET “CHEHAIMA AND NAIMA”, ON THE FATTY ACID COMPOSITION OF THE "BICEPS FEMORIS" MUSCLE OF LAMBS

This work aimed to assess the impact of a diet based on green pastures of steppe rangelands and another based on a concentrate diet, on the composition of the intramuscular lipid fraction of different sections of the biceps femoral muscle of lambs, in two regions of the wilaya of Tiaret, in winter and in spring. The biceps femoral muscle sample collection came from 20 different lamb carcasses (10 samples for each of our two study regions, with 05 samples for each season). These samples were taken on different occasions in large butcher shops. Total SFA content in both regions shows dominance in lambs from the regimen concentrate, that C 16: 0 and C 18: 0 are the most dominant. There is no remarkable difference in total MUFA content, and on individual monounsaturated FA, between the two regimens. In both regions, the most dominant FAs in the intramuscular lipid fraction of pasture lambs are C18: 3n3 followed by C18: 2n6 cis-9, C20: 4n6, C20: 5n3, and C22: 6n3, compared to those from concentrate regimen; however, lambs from Chehaima concentrate regimen had the most dominant FA in their intramuscular fat, C18: 3n6 followed by C20: 4n6, compared to those from pastures of Naima. The values of the ratio n-6 / n-3 correspond to the nutritional recommendations. The feed ratio ΣPUFA / ΣSFA of lambs from pastures in the Chehaima region is the only one that corresponds to the desired value. Based on this research, it is concluded that lambs that have grazed grass in steppe rangelands have the richest meat in omega 3, which is more desirable and benefits for human health.

New parameters of lifetime assessment of meat productivity of Dzhalginskiy merino sheep

For breeding work in order to increase productivity indicators in sheep, it is necessary to use the most informative exterior parameters. This dictates the need to develop new approaches to assessing phenotypic parameters. Purpose. Development of new parameters for live assessment of meat productivity of sheep of the Dzhalginskiy merino breed and study of their significance using the method of principal components. Scientific novelty. We have proposed several new parameters for the lifetime assessment of the parameters of meat productivity and studied the possibility of their use in sheep of the Dzhalginskiy merino breed. Methods. The possibility was studied for life determination of the size of individual muscle groups using such parameters as the girth of the shoulder, forearm and thigh by instrumental methods, as well as measuring the parameters of the muscle eye, the thickness of the femoral muscle and fat using ultrasound. To assess the significance of the proposed parameters, in comparison with those used in existing practice, we applied the principal component method and correlation analysis. Results. Five main components have been established, which determine 70, 3% of the total phenotypic variability in sheep of the Dzhalginskiy merino breed. They characterize the size of the forelimbs, the size of the chest, the parameters of the muscular eye, the size of the hind limbs and the thickness of fat in the lumbar region. The parameters of the shoulder, forearm and hip girth we proposed showed high significance in the calculation of the components. The thickness of the femoral muscle was found to be of little significance. It was also found that most of the measurements used do not reliably correlate with each other. High positive significant correlations were found only between body weight and average daily weight gain, thickness and width of the muscle eye, shoulder girth and height at the sacrum. Thus, we have found that for the phenotypic assessment of the Dzhalginskiy merino sheep, it is advisable to use the girth of the shoulder, forearm and thigh as additional parameters.

Danon disease: a case report and literature review

Background Danon disease (DD) is a rare x-linked dominant multisystemic disorder with a clinical triad of severe cardiomyopathy, skeletal myopathy, and mental retardation. It is caused by a defect in the lysosomal-associated membrane protein-2 (LAMP2) gene, which leads to the formation of autophagic vacuoles containing glycogen granule deposits in skeletal and cardiac muscle fibers. So far, more than 50 different mutations in LAMP2 have been identified. Case presentation Here, we report an 18-year-old male patient who was hospitalized for heart failure. Biopsy of the left lateral femoral muscle revealed scattered autophagic vacuoles in the muscle fibers with increased glycogen. Next generation sequencing (NGS) was used to detect gene mutations of the proband sample and a novel frameshift mutation (c.1052delG) has been identified in exon 8 of LAMP2, which leads to truncation of the protein. Conclusion We found a novel frameshift mutation, a hemizygous mutation (c.1052delG) in exon 8 of LAMP2, identified as presenting the hypertrophic cardiomyopathy (HCM) phenotype. Genetic analysis is the gold standard for the diagnosis of DD and is essential to determine appropriate treatment strategies and to confirm the genetic risk of family members.

Influence of low-molecular peptides isolated from mare’s milk on the performance of rats in case of physical exercise

In an experiment on rats on the «Treadmill running» model, the performance indicators were studied, as well as the state of the antioxidant defense system, including the level of under-oxidized products in the blood serum, erythrocyte membranes and the mitochondrial fraction of the femoral muscle of animals against the background of 28-day intake of the fraction of non-molecular peptides isolated from mare's milk. In the experimental group, in comparison with the control animals, the running time on the treadmill on the 28th day increased by 73.5 %, while a positive trend of increasing the run time has been noted since the seventh day of training, gradually increasing to the 28th day of the training. The intake by animals of the enriched fraction of low molecular weight peptides against the background of physical activity was accompanied by a decrease in the MDA level in the membranes of erythrocytes by 25.3 %, and diene conjugates by 33.8 %, as well as the activation of superoxide dismutase and catalase in the membranes of erythrocytes. A similar change was found in the mitochondrial fraction of the femoral muscle of animals. The experimental group of rats showed a lower concentration of lactic acid in the blood serum by 40.6 %; and in the femoral muscle by 24.7 %. Pyretic acid content was not different from the initial values. These results substantiate the usefulness of low molecular weight peptides in the design of specialized products and dietary supplements for sports nutrition.

Efficacy of systemic administration of riboflavin on a rabbit model of corneal alkali burn

Changes in the barrier mechanisms in the eye should determine the rational route for the administration and dosage of each drug in the treatment of traumatic injuries and other pathologies. The aim of this study was to examine the efficacy of intra-arterial delivery of 14C-riboflavin (as an “indicator”) and compare it with intravenous and intramuscular administration in an animal model of chemical eye burn. 14C-riboflavin (14C-I) was administered by intra-arterial (carotid artery), intravenous (femoral vein) and intramuscular (femoral muscle) routes. The total radioactivity was determined over 2 h in the plasma and structures of the rabbit’s eyes using a scintillation counter. The results of the study show that intravascular administration of 14C-I gives significantly higher concentrations of total radioactivity in the blood and is accompanied by a significant increase in the permeability of the blood-barrier and barrier in eyes suffering from burns. The highest concentration in the plasma and aqueous humour of the anterior chamber of the eye was observed during the first hour with the intra-arterial route of administration of 14C-I in either burnt and unburnt eyes. The distribution of total radioactivity in the structures of the eye over the 2 h of the experiment showed a higher level of the drug under intra-arterial administered in the uveal regions, namely: the iris, ciliary body, choroid, retina and also the sclera and cornea. This experimental model shows that intra-arterial administration can increase the bioavailability of a drug to the structures of the eye within a short period of time.

Meat receiving with functional properties when feeding the rabbits and broiler chickens of metal and protein complexes

The results of researches on the infl uence of new metal and protein compounds in the feeding of rabbits and broiler chickens on the quality of meat have been presented in the paper. The researches have been aimed at solving the urgent problem of providing the population of country with complete-fl edged diets, which today account for up to 30 % of meat products. The paper shows that a lifetime change in the chemical composition and nutritional value of muscle tissue, obtaining functional foods is possible when correcting the micromineral part of the diet of animals through the use of metal and protein complexes Belmin and Chrombelmin. The use of modern research methods allowed us to determine the optimal rate of feeding these feed additives. The results have been shown that the inclusion in the diet of rabbits organic complex Belmin in an amount of 2,0 dose per head/day has increased content in muscle tissue of rabbits of iron (+37,8 %, P < 0,001), copper (+34,66 %, P < 0,001), zinc (+27,01 %, P < 0,001) and essential amino acids (+5,1–0,53 %). Feeding Chrombelmin to broiler chickens as part of compound feed (0,15 %) has increased the level of selenium in the chest muscle by 3 times (P < 0,001), in the femoral muscle – by 1,25 times. The content of manganese, zinc, and chromium changes slightly. In the breast muscle of broilers the lipid content has been increased by 1,37 % (P < 0,05), and in the femoral muscle it decreased by 1,11 % (P < 0,05). The use of metal and protein feed additives Belmin and Chrombelmin in animal husbandry increases productivity, as well as the production of meat products with the increase of content of micronutrients, which will provide the population with not only high-quality, but also useful for health food products.

Effect of Three Therapeutic Methods of Exercise, Ozone, and Stem Cells on the MEF2C Expression and Myostatin Levels in Femoral Muscle Tissue of the Osteoarthritis Rats

Aims: Myostatin and Myocyte Enhancer Factor 2C (MEF2C) are involved in muscle changes associated with bone problems. The aim of the present study was to determine the effect of three therapeutic methods of exercise, ozone, and stem cells on MEF-2C gene expression and myostatin levels of femoral muscle tissue in osteoarthritis rats. Methods & Materials: This experimental study was done on 63 male rats (mean age of 8-12 weeks and weight of 250-300 g). They were randomly divided into nine groups: the healthy control and osteoarthritis group and seven intervention groups of osteoarthritis rats, including , saline, exercise, ozone therapy, MSCs, MSCs+ozone therapy, exercise+ozone therapy, and exercise+MSCs. Osteoarthritis was induced in rats by surgery. The training program consisted of 30 min of running on a non-slip treadmill at a speed of 16 m/min. Rats in the MSCs group received 1×106 cells/kg. The ozone was injected into the articular line of the knee tibiofemoral at a concentration of 20 μg/mL. Tissue levels of myostatin were measured by enzyme-linked immunosorbent assay kits and MEF2C gene expression was measured by real-time polymerase chain reaction method. Findings: Cell+exercise, exercise+ozone, and cell+ozone groups showed a significant increase in MEF2C gene expression and a significant decrease in myostatin levels compared with the cell, exercise, and ozone groups (P<0.05). In the exercise+cell group, these changes were greater. Conclusion: The results showed that the combination of exercise and MSCs with an increase in MEF2C gene expression and a decrease in myostatin levels, can possibly have beneficial effects on the stimulatory and inhibitory factors of interactions between muscle and bone in the osteoarthritis rats, and ultimately reduce the risks of muscle weakness due to osteoarthritis complications.