Isolation and identification of nitrogen-fixing Enterobacter cloacae and Klebsiella planticola associated with rice plants

1983 ◽  
Vol 29 (10) ◽  
pp. 1301-1308 ◽  
Author(s):  
J. K. Ladha ◽  
W. L. Barraquio ◽  
I. Watanabe
Keyword(s):  
Heterotrophic Bacteria ◽  
Enterobacter Cloacae ◽  
Dry Weight ◽  
Nitrogen Fixing ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Cross Reactions ◽  
Rice Plants ◽  
Klebsiella Planticola ◽  
Percentage Incidence

Acid- and gas-producing nitrogen-fixing bacteria associated with rice roots and leaf sheaths were isolated. These isolates along with reference enterobacteria strains were characterized biochemically and serologically. Using selected cultural and biochemical tests, all isolates from rice were identified as Enterobacter cloacae, except two strains which were found similar to Klebsiella pneumoniae. However, further biochemical tests showed that the Klebsiella isolates were K. planticola. Antisera and fluorescent antibodies (FA) were prepared against K. pneumoniae M5a1, K. planticola DWUL2, and E. cloacae EnSs. The results of FA cross-reactions of 28 strains isolated from rice plants and of other type cultures showed a separation into two different serogroups: E. cloacae and K. planticola. However, the cross-reactions in gel immunodiffusion were found to be extensive and widespread. All strains of Enterobacteriaceae tested produced at least one immunodiffusible precipitin band with any one or all antisera. The population of N2-fixing enterobacteria associated with the root and stem of rice fell within the range of 103 and 105 per gram dry weight. The percentage incidence of N2-fixing enterobacteria among the aerobic heterotrophic bacteria in the root and stem was found to be less than 1%.

scholarly journals Vegetative growth response of upland rice to Actinomycetes, Azospirillum and Azotobacter

2021 ◽  
Vol 911 (1) ◽  
pp. 012060
Author(s):  
Agung Gunawan ◽  
Yusminah Hala ◽  
Alimuddin Ali ◽  
Oslan Jumadi ◽  
Muhammad Junda
Keyword(s):  
Plant Height ◽  
Vegetative Growth ◽  
Rice Plant ◽  
Upland Rice ◽  
Dry Weight ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Total N ◽  
Rice Plants ◽  
Wet Weight

Abstract The research aims is to determine the suitability of nitrogen fixing bacteria, namely Actinomycetes, Azospirillum and Azotobacter with upland rice seeds to the speed of radicle formation and growth of upland rice plants. Upland rice plant growth measurement parameters include; speed of formation of radicle length, upland rice plant height, number of upland rice tillers, dry weight of the top of upland rice plants and roots of upland rice plants, wet weight of upland rice plants and roots of upland rice plants, and total N of upland rice plants and upland roots Testing the application of N2 fixing bacteria on upland rice plants on a laboratory scale was carried out to determine the suitability of microbes with upland rice plant seeds in vitro. The pot test was carried out to determine the suitability of the N2 fixing bacteria with the vegetative growth of upland rice plants in vivo. Data were analyzed using ANOVA with Duncan’s advanced test. The results showed that upland rice plants inoculated with Actinomycetes, Azospirillum and Azotobacter showed significantly different growth from upland rice plants without nitrogen fixing bacteria treatment, namely the radicle formation speed and radicle length, plant height, number of tillers, wet weight, dry weight, and total N (%) plants. It can be concluded that the inoculation of nitrogen-fixing bacteria on upland rice plants has a significant effect on plant vegetative growth parameters and plant nitrogen content.

scholarly journals Isolation and Identification of Ssome Lactobacillus Spp. Bacteria and Evaluation Their Efficacy in The Management of Damping off Disease on Peas

2021 ◽  
Vol 910 (1) ◽  
pp. 012106
Author(s):  
Duha Faisal Ajaj ◽  
Abdullah Abdulkarim Hassan
Keyword(s):  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
Dry Weight ◽  
Root Systems ◽  
Damping Off ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Local Cultivar ◽  
Lactobacillus Spp ◽  
Significant Superiority

Abstract Twenty-eight isolates of Lactobacillus bacteria were isolated from the rhizosphere of pea plants grown in the fields of five districts in Salah al-Din, which included: Tikrit, Al-Alam, Al-Sharqat, Samarra and Baiji, diagnosed according to phenotypic and biochemical tests. Results showed the effect of L. paralimentarius 1081 on vegetative growth characteristics. Treatment of (bacterial filtrate + Ridomil in the presence of pathogenic fungi) was recording the highest values in dry weight of the vegetative and root systems, was 3.91 and 1.23 g respectively in the local cultivar, compared with the lowest values was 2.60 g and 0.76 g respectively in the Syrian cultivar. All treatments inducing plant resistance compared with healthy plants, and the highest activity of the Peroxidase and Polyphenol oxides in the treatment of (Bacterial filtrate + ridomil in the presence of pathogenic fungi), were 1.08 and 1.33 units/ml in the local cultivar, compared to the Syrian cultivar were 0.015 and 0.013, respectively. Results showed a significant decrease in the severity of infection for all treatments compared to the pathogenic fungus treatment, and the lowest infection severity of the vegetative and root systems was recorded in the treatment of (Bacterial filtrate + ridomil in the presence of pathogenic fungi), which was 14.11 and 12.47% in the local cultivar. There was a significant superiority of all treatments in productivity parameters of pea compared to the treatment of pathogenic fungi only, the highest of those parameters including weight of pods and grains weight/plant were recorded in the treatment (Bacterial filtrate + ridomil in the presence of pathogenic fungi) for the local cultivar was 18.07 g and 14.04 g compared to 10.43 g and 8.20 g in the treatment of the Syrian cultivar with pathogenic fungi only.

The API 20B microtube system to aid in the identification of N2-fixing Bacillaceae

1987 ◽  
Vol 33 (6) ◽  
pp. 504-509 ◽  
Author(s):  
R. J. Rennie
Keyword(s):  
Heterotrophic Bacteria ◽  
General Procedure ◽  
Bacillus Species ◽  
Computer Assisted ◽  
Growth Media ◽  
Natural Environments ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Carbohydrate Utilization

N2-fixing bacterial isolates from soil have been identified successfully using the API 20E and 50E microtubes in conjunction with a computer-assisted biochemical profile search. The 20E and 50E result in few positive tests with N2-fixing Bacillaceae. Recently, a new battery of 20 microtube tests, the 20B, has been introduced for identifying aerobic, heterotrophic bacteria isolated from natural environments. This system was compared with the 20E and evaluated for N2-fixing bacillus species from soil. It was then used in conjunction with guanine-plus-cytosine analysis to identify the C-11-25 (5DN+) bacillus strain that fixes N2 in association with Canadian wheat cultivars. The API 20E and 20B gave similar results for many biochemical tests, but the 20E resulted in negative tests for carbohydrate utilization by bacillus strains. The use of different growth media or 0.85% NaCl to suspend these bacteria prior to inoculating the cupules did not alter the inability of the 20E in this manner. Carbohydrate utilization in the 20B system agreed well with that in the traditional utilization tests. Hence, I concluded that the API 20E is not suitable to evaluate carbohydrate utilization in N2-fixing Bacillaceae. The spore-forming, acetylene-reducing C-11-25 strain of bacillus was biochemically similar to the ATCC type culture of Bacillus polymyxa (strain 842). Its guanine-plus-cytosine ratio of 48.0 mol% was similar to that of B. polymyxa (43–46 mol%). A general procedure for the isolation and identification of N2-fixing Bacillus from soil is proposed.

scholarly journals Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil

2021 ◽  
Vol 14 ◽  
pp. 117863612110242
Author(s):  
Sonal Suman ◽  
Tanuja
Keyword(s):  
Contaminated Soil ◽  
Growth Parameters ◽  
Chemical Properties ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Bacterial Degradation ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
16S Rna ◽  
Degrading Bacteria

DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that Enterobacter cloacae had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that Enterobacter cloacae was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

The use of antibiotic resistance profiling as a means of tracing sources of fecal contamination in source waters

2010 ◽  
Vol 10 (2) ◽  
pp. 209-215
Author(s):  
M. S. Mthembu ◽  
P. T. Biyela ◽  
T. G. Djarova ◽  
A. K. Basson
Keyword(s):  
Antibiotic Resistance ◽  
Municipal Wastewater ◽  
Fecal Contamination ◽  
Source Tracking ◽  
Human Consumption ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
E Coli ◽  
Intestinal Pathogens ◽  
Source Waters

Fecal contamination of source waters and its associated intestinal pathogens continues to pose risks to public health although the extent and effect of microbial contamination of source waters gets very little attention in designing treatment plants in most developing countries. Coliform counts give an indication of the overall bacterial contamination of water and thus its safety for human consumption. However, their presence fails to provide information about the source of fecal contamination which is vital to managing fecal contamination problems in surface waters. This study explored the use of multiple antibiotic resistance (MAR) indexing as means of differentiating E. coli isolates from different sources. A total of 322 E. coli isolates were obtained from municipal wastewater and from fecal samples from domestic and wild animals. Conventional culture methods and standard chemical and biochemical tests were used for isolation and identification of E. coli. Isolates were assayed against 10 antibiotics using the micro-dilution technique. The results obtained generated antibiotic resistance profiles which were used to statistically group the isolates into different subsets. Correct source classification was obtained for 60% of human-derived and 95% non-human-derived E. coli respectively. These results indicate the validity of the usefulness of MAR indexing as a method of bacterial source tracking.

scholarly journals Observation of Vibrio mediterranei (Pujalte and Garay 1986) / Vibrio shiloi (Kushmaro et al. 2001) bacteria from skin ulcers of the cultured sea cucumber Holothuria poli (Delle Chiaje, 1823)

2021 ◽  
Vol 38 (3) ◽  
pp. 393-397
Author(s):  
Mustafa Tolga Tolon ◽  
Ulviye Karacalar ◽  
Caner Şirin
Keyword(s):  
Sea Cucumber ◽  
Reference Data ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Skin Ulceration ◽  
Skin Ulcers ◽  
Vibrio Shiloi ◽  
Serious Disease ◽  
Dominant Bacteria ◽  
Major Pathogens

Skin ulcer syndrome is frequently reported as a serious disease affecting the health, growth and mortality of stocks in sea cucumber aquaculture. In this study, bacteria isolated predominantly from skin ulcers of sea cucumber Holothuria poli (Delle Chiaje, 1823), a new candidate for aquaculture in the Mediterranean, were investigated. Morphological and biochemical tests, and molecular analysis methods were used to examine the dominant bacteria in the lesions of H. poli showing skin ulceration, peristome tumour and visceral ejection symptoms in rearing tanks. Present study is the first report for isolation and identification of Vibrio mediterranei (Pujalte and Garay 1986) (called also Vibrio shiloi Kushmaro et al. 2001) as a predominant gram-negative bacterium in the skin ulcers of H. poli. Reference data provided from the present study would lead to understand possible major pathogens causing skin ulceration syndrome and is crucial for the prophylaxis and treatment of such disease in holothuriculture.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Cekaman Garam NaCl danTeknik Aplikasi Azolla pada Tanaman Padi

2020 ◽  
Vol 25 (3) ◽  
pp. 349-355
Author(s):  
Fitri Krismiratsih ◽  
Sugeng Winarso ◽  
Slamerto Slamerto
Keyword(s):  
Salt Stress ◽  
Block Design ◽  
Ground Cover ◽  
Dry Weight ◽  
Soil Conditions ◽  
Application Technique ◽  
Rice Plants ◽  
Root Dry Weight ◽  
Randomized Block Design ◽  
Salinity Levels

Efforts to increase production potential can be carried out by extensification in a less productive saline land. Salinity is a major problem in the growth of most plants. Azolla is a plant that is sensitive to salinity, but if it is applied well, it can grow optimally at high salinity levels. The purpose of this study is to obtain an azolla application technique that is effective in increasing the adaptation of rice plants to NaCl saline soil conditions. The experimental design used was Randomized Block Design (RBD) with 2 factors and 3 replications. The first factor was the azolla application technique consisted of 3 levels: fresh azolla composted, fresh azolla immersed, and fresh azolla as a ground cover. The second factor was the levels of NaCl salt stress consisted of 4 levels: control DHL 0, 2, 4, and 8 dS m-1. The adaptation ability of rice plants based on variable plants height growth rate, number of tillers, strove dry weight, root dry weight, stomata density, leaf chlorophyll (SPAD), age of flowering, number of paddy grain, and harvest index. The results showed how to test content up to 2 dS m-1 which increased rice growth especially the application of azolla composted. Increasing stress to 4 and 8 dS m-1showed bad effects on vegetative, physiology, and yields of rice components. The stronger of salt stress the higher all plants growth variables except the age of flowering that actually showed the acceleration of flowering. Application of composted azolla can increase the root dry weight and azolla as a ground cover can increase the numbers of paddy grains.   Keywords: azolla, NaCl, rice, stress

scholarly journals Citrobacter braakii Yield False-Positive Identification as Salmonella, a Note of Caution

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2177
Author(s):  
Joanna Pławińska-Czarnak ◽  
Karolina Wódz ◽  
Magdalena Kizerwetter-Świda ◽  
Tomasz Nowak ◽  
Janusz Bogdan ◽  
...  
Keyword(s):  
False Positive ◽  
Salmonella Enterica ◽  
Meat Products ◽  
Animal Origin ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
False Positive Identification ◽  
Food Of Animal Origin ◽  
Meat Samples

Background: Globally, Salmonella enterica is one of the leading causes of foodborne illness in humans. Food of animal origin is obligatorily tested for the presence of this pathogen. Unfortunately, in meat and meat products, this is often hampered by the presence of background microbiota, which may present as false-positive Salmonella. Methods: For the identification of Salmonella spp. from meat samples of beef, pork, and poultry, the authorized detection method is PN-EN ISO 6579-1:2017-04 with the White–Kauffmann–Le Minor scheme, two biochemical tests: API 20E and VITEK II, and a real-time PCR-based technique. Results: Out of 42 presumptive strains of Salmonella, 83.3% Salmonella enterica spp. enterica, 14.3% Citrobacter braakii, and 12.4% Proteus mirabilis were detected from 180 meat samples. Conclusions: Presumptive strains of Salmonella should be identified based on genotypic properties such as DNA-based methods. The aim of this study was the isolation and identification of Salmonella spp. from miscellaneous meat sorts: beef, pork, and poultry.

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