LECITHINASE SYSTEMS IN SUGAR BEET, SPINACH, CABBAGE, AND CARROT

1954 ◽  
Vol 32 (5) ◽  
pp. 571-583 ◽  
Author(s):  
Morris Kates
Keyword(s):  
Sugar Beet ◽  
High Speed ◽  
Thermal Inactivation ◽  
Enzyme Concentration ◽  
Organic Phosphate ◽  
Water Soluble ◽  
Optimum Conditions ◽  
Carrot Root ◽  
Enzyme Systems ◽  
Influence Of Ph

Lecithinase activity of aqueous extracts of sugar beet, spinach, or cabbage leaves, and of carrot root was found to be associated entirely with the plastid fractions, separated by high-speed centrifugation. The supernatant cell sap–cytoplasm fractions were not only inactive but actually inhibitory. The rate of enzymatic liberation of choline from lecithin by all plastid fractions was found to be greatly increased by saturation with diethyl ether. The influence of pH, enzyme concentration, substrate concentration, and temperature on the rate of ether-activated choline liberation was studied and optimum conditions for the reaction were determined. Under optimum conditions, liberation of choline from lecithin by each of the plastid fractions was rapid and was accompanied by a much slower liberation of inorganic and water-soluble organic phosphate; liberation of phosphates was much greater with spinach than with the other species. Thermal inactivation and fluoride inhibition of the enzyme systems were also studied.

LECITHINASE SYSTEMS IN SUGAR BEET, SPINACH, CABBAGE, AND CARROT

1954 ◽  
Vol 32 (1) ◽  
pp. 571-583 ◽  
Author(s):  
Morris Kates
Keyword(s):  
Sugar Beet ◽  
High Speed ◽  
Thermal Inactivation ◽  
Enzyme Concentration ◽  
Organic Phosphate ◽  
Water Soluble ◽  
Optimum Conditions ◽  
Carrot Root ◽  
Enzyme Systems ◽  
Influence Of Ph

Lecithinase activity of aqueous extracts of sugar beet, spinach, or cabbage leaves, and of carrot root was found to be associated entirely with the plastid fractions, separated by high-speed centrifugation. The supernatant cell sap–cytoplasm fractions were not only inactive but actually inhibitory. The rate of enzymatic liberation of choline from lecithin by all plastid fractions was found to be greatly increased by saturation with diethyl ether. The influence of pH, enzyme concentration, substrate concentration, and temperature on the rate of ether-activated choline liberation was studied and optimum conditions for the reaction were determined. Under optimum conditions, liberation of choline from lecithin by each of the plastid fractions was rapid and was accompanied by a much slower liberation of inorganic and water-soluble organic phosphate; liberation of phosphates was much greater with spinach than with the other species. Thermal inactivation and fluoride inhibition of the enzyme systems were also studied.

HYDROLYSIS OF LECITHIN BY PLANT PLASTID ENZYMES

1955 ◽  
Vol 33 (1) ◽  
pp. 575-589 ◽  
Author(s):  
Morris Kates
Keyword(s):  
Phosphatidic Acid ◽  
Inorganic Phosphate ◽  
Organic Phosphate ◽  
Water Soluble ◽  
Lag Phase ◽  
Carrot Root ◽  
First Order ◽  
Spinach Chloroplasts ◽  
Egg Lecithin ◽  
Hydrolysis Of

Enzymatic liberation of choline from egg lecithin by plastid fractions from sugar beet, spinach, and cabbage leaves and from carrot root was a rapid, first order reaction (up to 70% hydrolysis), and was not preceded by a lag phase. None of the choline-containing products of lecithin degradation (lysolecithin, glycerylphosphorylcholine, or phosphorylcholine) lost choline on incubation with spinach chloroplasts. Inorganic phosphate liberation from lecithin by the plastids was preceded by a lag phase and was much slower than choline liberation. Spinach chloroplasts catalyzed the liberation of inorganic phosphate from L-α-phosphatidic acid and from L-α-glycerophosphate. The water-soluble organic phosphate liberated from lecithin by spinach chloroplasts was identified chromatographically as phosphorylcholine. The ether-soluble organic phosphate produced during the hydrolysis of egg lecithin by carrot plastids was isolated and identified as L-α-phosphatidic acid. These observations suggest that the enzymatic hydrolysis of lecithin by plant plastids involves the following reactions: (1) lecithin → L-α-phosphatidic acid + choline; (2) L-α-phosphatidic acid → inorganic phosphate + diglyceride and/or (3) L-α-phosphatidic acid → glycerophosphate + fatty acids and (4) glycerophosphate → inorganic phosphate + glycerol; and (5) lecithin → phosphorylcholine + diglyceride. The L-α-structure for egg lecithin was confirmed.

HYDROLYSIS OF LECITHIN BY PLANT PLASTID ENZYMES

1955 ◽  
Vol 33 (4) ◽  
pp. 575-589 ◽  
Author(s):  
Morris Kates
Keyword(s):  
Phosphatidic Acid ◽  
Inorganic Phosphate ◽  
Organic Phosphate ◽  
Water Soluble ◽  
Lag Phase ◽  
Carrot Root ◽  
First Order ◽  
Spinach Chloroplasts ◽  
Egg Lecithin ◽  
Hydrolysis Of

Enzymatic liberation of choline from egg lecithin by plastid fractions from sugar beet, spinach, and cabbage leaves and from carrot root was a rapid, first order reaction (up to 70% hydrolysis), and was not preceded by a lag phase. None of the choline-containing products of lecithin degradation (lysolecithin, glycerylphosphorylcholine, or phosphorylcholine) lost choline on incubation with spinach chloroplasts. Inorganic phosphate liberation from lecithin by the plastids was preceded by a lag phase and was much slower than choline liberation. Spinach chloroplasts catalyzed the liberation of inorganic phosphate from L-α-phosphatidic acid and from L-α-glycerophosphate. The water-soluble organic phosphate liberated from lecithin by spinach chloroplasts was identified chromatographically as phosphorylcholine. The ether-soluble organic phosphate produced during the hydrolysis of egg lecithin by carrot plastids was isolated and identified as L-α-phosphatidic acid. These observations suggest that the enzymatic hydrolysis of lecithin by plant plastids involves the following reactions: (1) lecithin → L-α-phosphatidic acid + choline; (2) L-α-phosphatidic acid → inorganic phosphate + diglyceride and/or (3) L-α-phosphatidic acid → glycerophosphate + fatty acids and (4) glycerophosphate → inorganic phosphate + glycerol; and (5) lecithin → phosphorylcholine + diglyceride. The L-α-structure for egg lecithin was confirmed.

Simultaneous Spectrophotometric Determination of Salbutamol by Coupling with Diazotized 4-Aminobenzoic acid

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Hind Hadi ◽  
Gufran Salim
Keyword(s):  
Pharmaceutical Preparations ◽  
Coupling Reaction ◽  
Dosage Forms ◽  
Water Soluble ◽  
Trace Determination ◽  
Aminobenzoic Acid ◽  
Optimum Conditions ◽  
Colored Product ◽  
Versus Concentration

A simple, rapid and sensitive spectrophotmetric method for trace determination of salbutamol (SAL) in aqueous solution and in pharmaceutical preparations is described. The method is based on the diazotization coupling reaction of the intended compound with 4-amino benzoic acid (ABA) in alkaline medium to form an intense orange, water soluble dye that is stable and shows maximum absorption at 410 nm. A graph of absorbance versus concentration indicates that Beer’s law is obeyed over the concentration range of 0.5-30 ppm, with a molar absorbtivity 3.76×104 L.mol-1 .cm-1 depending on the concentration of SAL. The optimum conditions and stability of the colored product have been investigated and the method was applied successfully to the determination of SAL in dosage forms.

Optimization of Solid Lipid Nanoparticles of Ezetimibe in Combination with Simvastatin Using Quality by Design (QbD)

2020 ◽  
Vol 10 (4) ◽  
pp. 404-418
Author(s):  
Kruti Borderwala ◽  
Ganesh Swain ◽  
Namrata Mange ◽  
Jaimini Gandhi ◽  
Manisha Lalan ◽  
...  
Keyword(s):  
Particle Size ◽  
Solid Lipid Nanoparticles ◽  
High Speed ◽  
Entrapment Efficiency ◽  
Lipid Nanoparticles ◽  
Water Soluble ◽  
Lipid Matrix ◽  
Solid Lipid ◽  
32 Factorial Design ◽  
Full Factorial Experimental Design

Background: The objective of this study was to develop solid lipid nanoparticles (SLNs) of poorly water soluble anti-hyperlipidemic drugs-Ezetimibe in combination with Simvastatin. Methods: This study describes a 32 full factorial experimental design to optimize the formulation of drug loaded lipid nanoparticles (SLN) by the high speed homogenization technique. The independent variables amount of lipid (GMS) and amount of surfactant (Poloxamer 188) were studied at three levels and arranged in a 32 factorial design to study the influence on the response variables- particle size, % entrapment efficiency (%EE) and cumulative drug release (% CDR) at 24 h. Results: The particle size, % EE and % CDR at 24 h for the 9 batches (B1 to B9) showed a wide variation of 104.6-496.6 nm, 47.80-82.05% (Simvastatin); 48.60-84.23% (Ezetimibe) and 54.64-92.27% (Simvastatin); 43.8-97.1% (Ezetimibe), respectively. The responses of the design were analysed using Design Expert 10.0.2. (Stat-Ease, Inc, USA), and the analytical tools of software were used to draw response surface plots. From the statistical analysis of data, polynomial equations were generated. Optimized formulation showed particle size of 169.5 nm, % EE of 75.43% (Simvastatin); 79.10% (Ezetimibe) and 74.13% (Simvastatin); 77.11% (Ezetimibe) %CDR after 24 h. Thermal analysis of prepared solid lipid nanoparticles gave indication of solubilisation of drugs within lipid matrix. Conclusion: Fourier Transformation Infrared Spectroscopy (FTIR) showed the absence of new bands for loaded solid lipid nanoparticles indicating no interaction between drugs and lipid matrix and being only dissolved in it. Electron microscope of transmission techniques indicated sphere form of prepared solid lipid nanoparticles with smooth surface with size approximately around 100 nm.

scholarly journals Enzyme optimization to reduce the viscosity of pitanga (Eugenia uniflora L.) juice

2016 ◽  
Vol 19 (0) ◽  
Author(s):  
Ricardo Schmitz Ongaratto ◽  
Luiz Antonio Viotto
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Time ◽  
Regression Models ◽  
Incubation Temperature ◽  
Correlation Coefficients ◽  
Enzyme Concentration ◽  
Optimum Conditions ◽  
Activity Time ◽  
Independent Variables

Summary The aim of this work was to separately evaluate the effects of pectinase and cellulase on the viscosity of pitanga juice, and determine the optimum conditions for their use employing response surface methodology. The independent variables were pectinase concentration (0-2.0 mg.g–1) and cellulase concentration (0-1.0 mg.g–1), activity time (10-110 min) and incubation temperature (23.2-56.8 °C). The use of pectinase and cellulase reduced the viscosity by about 15% and 25%, respectively. The results showed that enzyme concentration was the most important factor followed by activity time, and for the application of cellulase the incubation temperature had a significant effect too. The regression models showed correlation coefficients (R2) near to 0.90. The pectinase application conditions that led to the lowest viscosity were: concentration of 1.7 mg.g–1, incubation temperature of 37.6 °C and incubation time of 80 minutes, while for cellulase the values were: concentration of 1.0 mg.g-1, temperature range of 25 °C to 35 °C and incubation time of 110 minutes.

High‐speed laser chemical vapor deposition of copper: A search for optimum conditions

1989 ◽  
Vol 65 (6) ◽  
pp. 2470-2474 ◽  
Author(s):  
B. Markwalder ◽  
M. Widmer ◽  
D. Braichotte ◽  
H. van den Bergh
Keyword(s):  
Chemical Vapor Deposition ◽  
Vapor Deposition ◽  
High Speed ◽  
Chemical Vapor ◽  
Optimum Conditions ◽  
Laser Chemical Vapor Deposition

Preparation of Water-Soluble Carboxymethylated Lignin from Wheat Straw Alkali Lignin

2012 ◽  
Vol 550-553 ◽  
pp. 1293-1298 ◽  
Author(s):  
Lin Huo Gan ◽  
Ming Song Zhou ◽  
Xue Qing Qiu
Keyword(s):  
Wheat Straw ◽  
Acid Concentration ◽  
1H Nmr Spectroscopy ◽  
Water Soluble ◽  
Monochloroacetic Acid ◽  
Hydroxyl Group ◽  
Carboxyl Group ◽  
Optimum Conditions ◽  
Alkali Lignin ◽  
Carboxyl Group Content

Water-soluble carboxymethylated lignin (CML) was synthesized using wheat straw alkali lignin (WAL) in aqueous medium. The process of carboxymethylation was optimized with respect to the NaOH concentration, monochloroacetic acid concentration, reaction temperature and time. The optimized product has a yield of 80.47% and a carboxyl group content of 2.8231 mmol•g-1, respectively. The optimum conditions for carboxymethylation are NaOH concentration of 20.0% (wt%), monochloroacetic acid concentration of 37.5% (wt%), temperature of 70 °C and time of 90 min. The optimized CML was characterized by FTIR spectroscopy, 1H NMR spectroscopy and interfacial tension apparatus. The result shows that the substitution reaction of carboxymethylation occurs simultaneously in the phenolic hydroxyl group and aliphatic hydroxyl group in WAL. CML has the surface activity in water for industrial application as dispersant.

scholarly journals Kinetics of Enzymatic Hydrolysis of Southeast Sulawesi Sago Starch

2020 ◽  
pp. 53-61
Author(s):  
Ansharullah Ansharullah ◽  
Muhammad Natsir
Keyword(s):  
Enzymatic Hydrolysis ◽  
Maximum Velocity ◽  
Enzyme Concentration ◽  
Hydrolysis Rate ◽  
Sago Starch ◽  
Optimum Conditions ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Substrate Concentrations ◽  
Menten Constant

The aims of this study were to characterize the kinetics of enzymatic hydrolysis of sago starch, obtained from Southeast Sulawesi Indonesia. The enzyme used for hydrolysis was bacterial ∝-amylase (Termamyl 120L from Bacillus licheniformis, E. C. 3.2.1.1).  The method to determine the initial velocity (Vo) of the hydrolysis was developed by differentiation a nonlinear equation (NLE).  The Vo of the hydrolysis was measured at various pH (6.0, 6.5,and 7.0), temperatures (40, 60, 75 and 95oC), enzyme concentrations (0.5, 1.0, 1.5 and 2.0 µg per mL) and in the presence of 70 ppm Ca++. The optimum conditions of this experiment were found to be at pH 6.5 – 7.0 and 75oC, and the Vo increased with increasing enzyme concentration. The Vo values at various substrate concentrations were also determined, which were then used to calculate the enzymes kinetics constant of the hydrolysis, including Michaelis-Menten constant (Km) and maximum velocity (Vmax) using a Hanes plot.  Km and Vmax values were found to be higher in the measurement at pH 7.0 and 75oC. The Km values  at four  different combinations of pH and temperatures (pH 6.5, 40oC; pH 6.5, 75oC; pH 7.0, 40oC; pH 7.0, 75oC) were found to be 0.86, 3.23, 0.77 and 3.83 mg/mL, respectively; and Vmax values were 17.5, 54.3, 20.3 and 57.1 µg/mL/min, respectively. The results obtained showed that hydrolysis rate of this starch was somewhat low.

Saflufenacil Carryover Injury Varies among Rotational Crops

2012 ◽  
Vol 26 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Darren E. Robinson ◽  
Kristen E. McNaughton
Keyword(s):  
Sugar Beet ◽  
Growth Yield ◽  
Dry Weight ◽  
Sugar Beet Plant ◽  
Root Weight ◽  
Carrot Root ◽  
Field Corn ◽  
Yield And Quality ◽  
Weight Yield

Trials were established in 2007, 2008, and 2009 in Ontario, Canada, to determine the effect of soil residues of saflufenacil on growth, yield, and quality of eight rotational crops planted 1 yr after application. In the year of establishment, saflufenacil was applied PRE to field corn at rates of 75, 100, and 200 g ai ha−1. Cabbage, carrot, cucumber, onion, pea, pepper, potato, and sugar beet were planted 1 yr later, maintained weed-free, and plant dry weight, yield, and quality measures of interest to processors for each crop were determined. Reductions in dry weight and yield of all grades of cucumber were determined at both the 100 and 200 g ha−1rates of saflufenacil. Plant dry weight, bulb number, and size and yield of onion were also reduced by saflufenacil at 100 and 200 g ha−1. Sugar beet plant dry weight and yield, but not sucrose content, were decreased by saflufenacil at 100 and 200 g ha−1. Cabbage plant dry weight, head size, and yield; carrot root weight and yield; and pepper dry weight, fruit number and size, and yield were only reduced in those treatments in which twice the field corn rate had been applied to simulate the effect of spray overlap in the previous year. Pea and potato were not negatively impacted by applications of saflufenacil in the year prior to planting. It is recommended that cabbage, carrot, cucumber, onion, pepper, and sugar beet not be planted the year after saflufenacil application at rates up to 200 g ha−1. Pea and potato can be safely planted the year following application of saflufenacil up to rates of 200 g ha−1.

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