Effect of freeze–thaw cycles on the freezing characteristics of a clayey silt at various overconsolidation ratios

1989 ◽  
Vol 26 (2) ◽  
pp. 217-226 ◽  
Author(s):  
J.-M. Konrad
Keyword(s):  
Void Ratio ◽  
Frost Heave ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Maximum Reduction ◽  
Clayey Silt ◽  
Wide Range ◽  
Repeated Freezing ◽  
Segregation Potential ◽  
Overconsolidated Soils

Repeated freezing and thawing affect the structure of clayey silts over a wide range of consolidation ratios. While the overall void ratio of the thawed soil either decreased, as in lightly overconsolidated soils, or increased, as in heavily overconsolidated samples, freezing and thawing caused a reduction in segregation potential after each freeze–thaw event. All the changes occurred during the first three cycles. The maximum reduction in segregation potential was about 50% in all samples. The implications for frost heave predictions in the field are discussed. Key words: freeze–thaw, clayey silt, segregation potential, frost heave.

Influence of overconsolidation on the freezing characteristics of a clayey silt

1989 ◽  
Vol 26 (1) ◽  
pp. 9-21 ◽  
Author(s):  
J.-M. Konrad
Keyword(s):  
Hydraulic Conductivity ◽  
Void Ratio ◽  
Moisture Transfer ◽  
Frozen Soil ◽  
Frost Heave ◽  
Unfrozen Water ◽  
Initial Void Ratio ◽  
Clayey Silt ◽  
Segregation Potential ◽  
Lens Formation

Laboratory freezing tests were performed on a saturated clayey silt at various overconsolidation ratios (OCR) to establish the relationship between initial void ratio and stress history, and the amount of moisture transfer during freezing. The frost heave tests were analysed in terms of the segregation potential as well as a function of the temperature of ice lens formation and the overall hydraulic conductivity of the frozen fringe. All other factors being the same, the segregation potential was found to increase with increasing values of OCR (decreasing initial void ratios). However, the combined effects of decreasing void ratio and increasing suction at the frost line, all other factors being identical in all freezing tests, resulted in decreasing segregation potentials. This trend was the result of a decrease in the temperature of ice lens formation and the concomitant decrease in overall hydraulic conductivity of the frozen fringe. A simple model showed that the capillary unfrozen water between clay particles increases when the particles pack closer together, as overconsolidation increases, allowing the migratory water to freeze within the frozen soil at a colder temperature. Key words: frost heave, clayey silt, overconsolidation, void ratio.

Permeability and volume changes in till due to cyclic freeze/thaw

1998 ◽  
Vol 35 (3) ◽  
pp. 471-477 ◽  
Author(s):  
Peter Viklander
Keyword(s):  
Soil Structure ◽  
Void Ratio ◽  
Vertical Direction ◽  
Rigid Wall ◽  
Freezing And Thawing ◽  
Volume Changes ◽  
Freeze Thaw ◽  
Fine Grained ◽  
Loose Soil ◽  
Freezing And Thawing Cycles

A fine-grained nonplastic till was compacted in the laboratory in three types of rigid wall permeameters, having a volume of 0.4, 1.5, and 25 dm3, respectively, and, was thereafter exposed to a maximum of 18 freezing and thawing cycles. The permeabilities in the vertical direction of saturated samples were measured in unfrozen soil as well as in thawed soil. The results show that the permeabilities changed after freezing and thawing. The magnitude of the changes in this study were in the range 0.02-10 times after freeze/thaw compared with the unfrozen soil. Soil exhibited volume changes subsequent to freeze/thaw. The volume typically decreased for an initially loose soil and increased for a dense soil. Independent of whether the initial soil structure was loose or dense, a constant "residual" void ratio, eres, was obtained after 1-3 cycles. For the soil investigated, the residual void ratio ranged from 0.31 to 0.40.Key words: till, fine-grained, non plastic, permeability, freeze/thaw, residual void ratio.

FREEZE-THAW EFFECTS ON GRANULAR STRUCTURE REORGANIZATION FOR SOIL MATERIALS OF VARYING TEXTURE AND MOISTURE CONTENT

1988 ◽  
Vol 68 (3) ◽  
pp. 485-494 ◽  
Author(s):  
S. PAWLUK
Keyword(s):  
Moisture Content ◽  
Lacustrine Sediments ◽  
Field Capacity ◽  
Granular Structure ◽  
Glacial Till ◽  
Microstructural Development ◽  
Clay Loam ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Repeated Freezing

Repeated freezing and thawing of glacial till cores of clay loam texture results in the formation of granic and metafragmic microfabrics. These units of fabric are best developed near the surface of cores kept at moisture levels between field capacity and saturation. Well-sorted lacustrine sediments with fewer voids tend to form banded fabrics. Many of the morphological features such as vesicles, metavughs and desiccation cracks commonly attributed to freeze-thaw processes are evident in all materials tested. Discrete units of fabric observed in this study are very similar to units of fabric observed in the Ah horizons of Black Chernozemic and Cryosolic soils. Results of this investigation strongly support earlier research which suggests that frost processes are major contributors to their microstructural development. Key words: Granic, freeze-thaw, microfabrics

Segregation potential – pressure – salinity relationships near thermal steady state for a clayey silt

1990 ◽  
Vol 27 (2) ◽  
pp. 203-215 ◽  
Author(s):  
J.-M. Konrad
Keyword(s):  
Steady State ◽  
Pore Water ◽  
Moisture Transfer ◽  
Frost Heave ◽  
Water Salinity ◽  
Saline Soils ◽  
Clayey Silt ◽  
Segregation Potential ◽  
Pore Water Salinity ◽  
Thermal Steady State

Laboratory freezing tests were performed on a saturated clayey silt at various pore-water salinities and applied pressures to establish the relationships between pore-water salinity, overburden, and the amount of moisture transfer during freezing near thermal steady state conditions. The experimental data confirmed that the concept of segregation potential established for salt-free soils can be extended to saline soils. The segregation potential at the onset of the final ice lens in step-freezing tests (or near thermal steady state) should be related to the average salinity of the frozen fringe associated with the final ice lens. This pore-water salinity is different from the initial pore-water salinity as a result of solute exclusion at the ice lenses causing an enrichment as freezing proceeds. Unfortunately, it is very difficult to determine the average salinity in the frozen fringe owing to its small size. Instead, it is proposed to use the initial water salinity to develop the constitutive equations in freezing saline soils. A procedure for frost heave predictions in the field is outlined. Key words: freezing tests, clayey silt, saline water, frost heave.

Inactivation of a Cold-Induced Putative RNA Helicase Gene of Listeria monocytogenes Is Accompanied by Failure To Grow at Low Temperatures but Does Not Affect Freeze-Thaw Tolerance

2010 ◽  
Vol 73 (8) ◽  
pp. 1474-1479 ◽  
Author(s):  
REHA O. AZIZOGLU ◽  
S. KATHARIOU
Keyword(s):  
Listeria Monocytogenes ◽  
Normal Growth ◽  
Rna Helicase ◽  
Soft Agar ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Transposon Insertion ◽  
Helicase Gene ◽  
Repeated Freezing ◽  
Cold Sensitive

Freeze-thaw tolerance (cryotolerance) of Listeria monocytogenes is markedly influenced by temperature of growth of the bacteria, and may involve responses to low-temperature stresses encountered during freezing and thawing. A cold-sensitive mariner-based transposon mutant of L. monocytogenes F2365 was found to harbor a single insertion in LMOf2365_1746, encoding a putative RNA helicase, and earlier shown by other investigators to be induced during 4°C growth of L. monocytogenes. The mutant had normal growth at 37°C but completely failed to grow at either 4 or 10°C, and had impaired growth and reduced swarming on soft agar at 25°C. However, the mutation had no discernible influence on the ability of the bacteria to tolerate repeated freezing and thawing after growth at either 25 or 37°C. The findings suggest that the transposon insertion in the putative helicase gene, in spite of the severely cold-sensitive phenotype that accompanies it, does not affect the ability of the bacteria to cope with cold-related stresses encountered during repeated freezing and thawing.

scholarly journals Role of Growth Temperature in Freeze-Thaw Tolerance of Listeria spp.

2009 ◽  
Vol 75 (16) ◽  
pp. 5315-5320 ◽  
Author(s):  
Reha O. Azizoglu ◽  
J. Osborne ◽  
S. Wilson ◽  
S. Kathariou
Keyword(s):  
Growth Temperature ◽  
Negative Impact ◽  
Processing Plant ◽  
Virulence Determinants ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Food Borne ◽  
Wide Range ◽  
Different Temperatures ◽  
The Impact

ABSTRACT The food-borne pathogen Listeria monocytogenes can grow in a wide range of temperatures, and several key virulence determinants of the organism are expressed at 37°C but are strongly repressed below 30°C. However, the impact of growth temperature on the ability of the bacteria to tolerate environmental stresses remains poorly understood. In other microorganisms, cold acclimation resulted in enhanced tolerance against freezing and thawing (cryotolerance). In this study, we investigated the impact of growth temperature (4, 25, and 37°C) on the cryotolerance of 14 strains of L. monocytogenes from outbreaks and from food processing plant environments and four strains of nonpathogenic Listeria spp. (L. welshimeri and L. innocua). After growth at different temperatures, cells were frozen at −20°C, and repeated freeze-thaw cycles were applied every 24 h. Pronounced cryotolerance was exhibited by cells grown at 37°C, with a <1-log decrease after 18 cycles of freezing and thawing. In contrast, freeze-thaw tolerance was significantly reduced (P < 0.05) when bacteria were grown at either 4 or 25°C, with log decreases after 18 freeze-thaw cycles ranging from 2 to >4, depending on the strain. These findings suggest that growth at 37°C, a temperature required for expression of virulence determinants of L. monocytogenes, is also required for protection against freeze-thaw stress. The negative impact of growth at low temperature on freeze-thaw stress was unexpected and has not been reported before with this or other psychrotrophic microorganisms.

Freeze/thaw stability of transferrin, and reference values obtained with kinetic nephelometry.

1984 ◽  
Vol 30 (1) ◽  
pp. 114-115 ◽  
Author(s):  
L Stoddard ◽  
W Dennis ◽  
R M Parvin ◽  
O W van Assendelft
Keyword(s):  
Reference Values ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Normal Reference ◽  
Repeated Freezing ◽  
Blood Specimens ◽  
Normal Adults

Abstract To determine whether transferrin is stable to repeated freezing and thawing, we used blood specimens from 100 normal adults. Their transferrin concentrations were determined by a laser kinetic nephelometric immunoassay. The sera were stored at 4 degrees C and analyzed the next day (day 1). Specimens 1-25 were stored at -20 degrees C and assayed again on days 3, 4, 8, 16, 22, and 43. Specimens 26-100 were stored at 4 degrees C and analyzed again on day 2. Transferrin proved to be stable to repeated freezing and thawing. Normal reference values with respect to sex and age are given, as determined with the Baker 420 Immunological Nephelometer.

scholarly journals Evaluation of the Strength Characteristics of Silty-Clayey Soils during Freezing-Thawing Cycles

2022 ◽  
Vol 12 (2) ◽  
pp. 802
Author(s):  
Elena Bragar ◽  
Yakov Pronozin ◽  
Askar Zhussupbekov ◽  
Alexander Gerber ◽  
Assel Sarsembayeva ◽  
...  
Keyword(s):  
Bearing Capacity ◽  
Void Ratio ◽  
Least Squares Method ◽  
Initial Moisture Content ◽  
Strength Characteristics ◽  
Frost Heave ◽  
Clayey Soils ◽  
Angle Of Internal Friction ◽  
Freeze Thaw ◽  
Approximation Coefficients

Destructuring settlements due to frost heave during the structures’ exploitation are often not taken into account at the designing stage, although they are indirectly related to the bearing capacity of the soils. The objective of this research was analyzing the effect of the number of freezing-thawing cycles on the strength characteristics of soils. A paired experiment with various initial parameters (void ratio, initial moisture content, and the number of freezing-thawing cycles) was carried out. According to the experimental results, the cohesion largely depends on the above parameters which might lead to its decrease by up to three times. The angle of internal friction demonstrated an indefinite behavior during the freeze-thaw cycles, which is confirmed by a literature review. Freezing–thawing cycles significantly decrease the soil bearing capacity: up to 44% after 10 freezing-thawing cycles for soil with and . However, in the case of and it increased by 33%. A program based on the least-squares method was used to calculate the approximation coefficients of the dependence describing the changes in strength characteristics from the abovementioned parameters. Changes in strength characteristics must be taken into account when designing structures, as they can lead to additional settlement or even subsidence of the foundations.

scholarly journals Glacial and Periglacial Transformation of Palaeokarst in the Lublin-Volhynia Region (Se Poland, NW Ukraine) on the Base of TL Dating

2007 ◽  
Vol 27 (-1) ◽  
pp. 41-46 ◽  
Author(s):  
Radosław Dobrowolski ◽  
Stanisław Fedorowicz
Keyword(s):  
Genetic Relationship ◽  
Experimental Investigations ◽  
Considerable Influence ◽  
Freezing And Thawing ◽  
Tl Dating ◽  
Freeze Thaw ◽  
Se Poland ◽  
Repeated Freezing ◽  
Formation Conditions ◽  
Close Genetic Relationship

Glacial and Periglacial Transformation of Palaeokarst in the Lublin-Volhynia Region (Se Poland, NW Ukraine) on the Base of TL Dating Distinctly diverse results of TL dating are obtained for the deposits with similar lithofacial features but filling morphologically differentiated karst palaeoforms (dolines, pipes, pockets). The infillings of dolines and pipes are mostly of the Saalian age. Based on sedimentological analysis, their formation conditions are related to sub- or/and terminoglacial environment. The age obtained for all infillings of pockets is underestimated in comparison with lithostratigraphic data. A close genetic relationship between these forms and periglacial conditions seems to indicate that the reduction of TL signal is mostly influenced by the disintegration of grains resulted from the repeated freezing and thawing of glaciogenic deposits. A considerable influence of frost weathering on the decrease of thermoluminescence intensity of mineral grains is indirectly confirmed by the results of experimental investigations consisting in the repeated TL measurements of pockets' infillings after successive freeze-thaw cycles.

scholarly journals PSIV-25 Effect of Various Handling Methods of Plasma and Serum Samples on Pregnancy Associated Glycoprotein Concentrations

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 289-290
Author(s):  
Grace M Wesson ◽  
Lohana Fernandez ◽  
Rebecca K Poole ◽  
Gessica A Franco ◽  
Sydney T Reese ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Plasma Samples ◽  
Freezing And Thawing ◽  
Serum Samples ◽  
Freeze Thaw ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Repeated Freezing ◽  
Pregnancy Status ◽  
Handling Methods

Abstract Pregnancy associated glycoproteins (PAG) can be used as a biomarker for early pregnancy diagnosis, so accurate and consistent PAG detection is critical. The objective of this study was to determine if plasma and serum PAG concentrations were altered when centrifugation occurred at different times post-collection, when subjected to repeated freezing and thawing, and when monoclonal antibodies were kept in frequently or infrequently opened containers. Plasma (n = 4) and serum (n = 4) samples were collected from two open cows and two pregnant cows 28 days after artificial insemination. Pregnancy status was determined via transrectal ultrasonography. Plasma and serum samples were evenly separated and either centrifuged on the day of collection, or placed at 4°C and centrifuged the next day. An in-house PAG ELISA was performed on all samples before freezing (NOTHAW), after being frozen for one week (INTACT), after one freeze/thaw cycle (THAW1), two freeze/thaw cycles (THAW2), and three freeze/thaw cycles (THAW3). Data were analyzed using one-way ANOVA (GLM procedure, SAS 9.4). All samples from open cows were below the baseline of the assay. For pregnant cows, plasma samples had greater PAG concentrations than serum samples (11.84 vs 3.30 ± 0.66 ng/mL, respectively, P &lt; 0.05). No differences were observed for day of centrifugation in both plasma and serum samples (P = 0.50 and P = 0.60, respectively) and in handling of monoclonal antibodies (P = 0.90). Freezing and thawing did not impact PAG concentrations in plasma samples (P = 0.19), but did alter serum concentrations (P = 0.01). Specifically, THAW1 (1.98 ng/mL) and THAW2 (1.42 ng/mL) serum PAG concentrations were lower compared to NOTHAW, THAW3, and INTACT samples (4.66, 4.85, and 3.57 ng/mL, respectively). Based on these data, plasma yields more consistent results than serum, even after several freeze-thaw cycles, and handling of monoclonal antibodies or time of centrifugation has no significant effect on measured PAG.

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