STUDIES ON THE PRESERVATION OF BLOOD: VII. THE INFLUENCE OF INOSINE ON THE METABOLIC BEHAVIOR OF THE ERYTHROCYTE DURING THE PRESERVATION OF BLOOD IN THE COLD

1959 ◽  
Vol 37 (1) ◽  
pp. 69-79
Author(s):  
D. Rubinstein ◽  
S. Kashket ◽  
Rhoda Blostein ◽  
O. F. Denstedt
Keyword(s):  
Cold Storage ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Blood Samples ◽  
The Third ◽  
Duration Of Storage ◽  
The Difference ◽  
Blood Specimens ◽  
Metabolic Behavior ◽  
High Degree

Inosine, like other purine nucleosides when added to preserved blood specimens, induces a resynthesis of organic phosphate esters in the erythrocytes. Apart from the difference in rates of reaction, the metabolic reconstitution of the cells is the same at 37° and 4 °C. The reconstitution of the esters occurs rapidly even at 4 °C, and the higher the concentration of inosine added, the more prolonged is the maintenance of the esters. When inosine was added repeatedly to blood samples during storage, a phase of synthesis was induced with each addition of nucleoside. The capacity of the erythrocytes to resynthesize 2,3-diphosphoglyceric acid (DPG) remained normal regardless of the storage age of the sample but the capacity to replenish ATP decreased with the duration of storage. Addition of inosine at the end of the third week of cold storage can effect a high degree of restoration of metabolic viability in the cells at 4 °C within 24 hours.

STUDIES ON THE PRESERVATION OF BLOOD: VII. THE INFLUENCE OF INOSINE ON THE METABOLIC BEHAVIOR OF THE ERYTHROCYTE DURING THE PRESERVATION OF BLOOD IN THE COLD

1959 ◽  
Vol 37 (1) ◽  
pp. 69-79 ◽  
Author(s):  
D. Rubinstein ◽  
S. Kashket ◽  
Rhoda Blostein ◽  
O. F. Denstedt
Keyword(s):  
Cold Storage ◽  
Organic Phosphate ◽  
Phosphate Esters ◽  
Blood Samples ◽  
The Third ◽  
Duration Of Storage ◽  
The Difference ◽  
Blood Specimens ◽  
Metabolic Behavior ◽  
High Degree

Inosine, like other purine nucleosides when added to preserved blood specimens, induces a resynthesis of organic phosphate esters in the erythrocytes. Apart from the difference in rates of reaction, the metabolic reconstitution of the cells is the same at 37° and 4 °C. The reconstitution of the esters occurs rapidly even at 4 °C, and the higher the concentration of inosine added, the more prolonged is the maintenance of the esters. When inosine was added repeatedly to blood samples during storage, a phase of synthesis was induced with each addition of nucleoside. The capacity of the erythrocytes to resynthesize 2,3-diphosphoglyceric acid (DPG) remained normal regardless of the storage age of the sample but the capacity to replenish ATP decreased with the duration of storage. Addition of inosine at the end of the third week of cold storage can effect a high degree of restoration of metabolic viability in the cells at 4 °C within 24 hours.

scholarly journals ON STUDY OF BOVINE PYROPLASMOSIS IN THE LOWLAND AND FOOTHILL ZONES OF ARMENIA

2021 ◽  
pp. 117-122
Author(s):  
Vardanyan ◽  
Movsesyan ◽  
Petrosyan ◽  
Nykogosyan
Keyword(s):  
Dominant Species ◽  
Natural Habitat ◽  
Clinical Signs ◽  
Dermacentor Marginatus ◽  
Boophilus Annulatus ◽  
The Third ◽  
The Difference ◽  
The Republic ◽  
High Degree ◽  
Species Being

In two landscape zones of the Republic of Armenia, the regularities of the course of one of the most common haemosporidiosis in cattle, piroplasmosis, were studied. Based on our research, a difference was established in the timing of the onset of the disease, the number of outbreaks, and the severity of clinical signs in animals of the lowland and foothill zones. We have proved that 4 outbreaks of the disease took place in the farms of the lowland zone: the first at the end of April after the attack of ticks on the animal. At the same time, the first symptoms of the disease were noted. The second outbreak of the disease and a high degree of ticks were recorded in the second half of July. The third outbreak was in early August and the fourth in autumn starting from the 10th of October. In contrast to the lowland, there were 3 outbreaks of piroplasmosis in the farms of the foothill zone, and they were not very pronounced. This phenomenon can be explained by the difference in the temperature regime of the natural habitat. In the lowland zone, 4 species of ticks were recorded with the dominant species being Boophilus annulatus rarely found in the foothill zone. In the foothill zone, the same species of ticks as in the lowland were identified, along with the species Dermacentor marginatus.

scholarly journals Excess mortality analysis for Germany for all three COVID-19 waves in 2020 - 2021

2021 ◽  
Author(s):  
Alexej Weber
Keyword(s):  
Excess Mortality ◽  
Third Wave ◽  
The Third ◽  
Baseline Mortality ◽  
Group Experiences ◽  
The Difference ◽  
Almost All ◽  
High Degree ◽  
The Impact ◽  
Second Wave

Background and Aims: The reported case and death numbers of the coronavirus disease (COVID-19) are often used to estimate the impact of COVID-19. We observe that during the second half of the first and second waves, the COVID-19 deaths are significantly higher than the excess mortality. We attribute the difference to the pre-dying effect. We then compare the excess mortality to the official COVID-19 death numbers and calculate the infection fatality rates (IFRs) and the percentage of infected individuals from excess mortality for different age bands. We also compare the impact of COVID-19 to past influenza waves and analyze the vaccination effect on excess mortality. Methods: We forecast the baseline mortality from official data on deaths in Germany. Distributing a part of excess mortality into the near future, we lower the baseline simulating the pre-dying effect. From there, we compare the excess mortality to official COVID-19 deaths. From the observed mortality deficit, we estimate the percentage of infected individuals and then estimate the age-dependent IFRs. Results: In the first wave, we find an overall excess mortality of ca. 8 000. For the second wave, the overall excess mortality adds up to ca. 56 000. We find, that the pre-dying effect explains the difference between the official COVID-19 deaths and excess mortality in the second half of the waves to a high degree. Attributing the whole excess mortality to COVID-19, we find that the IFRs are significantly higher in the second wave. In the third wave, we find an excess mortality in mid-age bands which cannot be explained by the official COVID-19 deaths. For the senior band 80+, we find results in favor of a strong and positive vaccination effect for the third COVID-19 wave. Conclusions: We conclude that in the first and second COVID-19 waves, the COVID-19 deaths explain almost all excess mortality when the pre-dying effect is taken into account. In the third wave in 2021, the excess mortality is not very pronounced for the 80+ age band, probably due to vaccination. The partially unvaccinated 40-80 age group experiences a pronounced excess mortality in the third wave while there are too few official COVID-19 deaths to explain the excess. The no-vaccination scenario for the 80+ age band results in a similarly high excess mortality as for the more younger age bands, suggesting a very positive vaccination effect on reduction of COVID-19 deaths.

Ragam Bangunan Perundang-Undangan Hukum Keluarga di Negera-Negara Muslim Modern: Kajian Tipologis

2018 ◽  
Vol 11 (1) ◽  
pp. 49-60
Author(s):  
Miftahul Huda
Keyword(s):  
Saudi Arabia ◽  
United Arab Emirates ◽  
Islamic Law ◽  
Family Law ◽  
Marriage Law ◽  
The Third ◽  
Muslim Countries ◽  
Fourth Type ◽  
Modern Family ◽  
The Difference

The reality of the difference in applying Islamic law in the context of marriage law legislation in modern Muslim countries is undeniable. Tunisia and Turkey, for example, have practiced Islamic law of liberal nuance. Unlike the case with Saudi Arabia and the United Arab Emirates that still use the application of Islamic law as it is in their fiqh books. In between these two currents many countries are trying to apply the law in their own countries by trying to bridge the urgent new needs and local wisdom. This is widely embraced by modern Muslim countries in general. This paper reviews typologically the heterogeneousness of family law legislation of modern Muslim countries while responding to modernization issues. Typical buildings seen from modern family law reforms can be classified into four types. The first type is progressive, pluralistic and extradoctrinal reform, such as in Turkey and Tunisia. The second type is adaptive, unified and intradoctrinal reform, as in Indonesia, Malaysia, Morocco, Algeria and Pakistan. The third type is adaptive, unified and intradoctrinal reform, represented by Iraq. While the fourth type is progressive, unifiied and extradoctrinal reform, which can be represented by Somalia and Algeria.

Ragam Qiraat Al-Qur'an

SUHUF ◽  
2015 ◽  
Vol 2 (1) ◽  
pp. 53-72
Author(s):  
Ahmad Fathoni
Keyword(s):  
The Third ◽  
The Difference ◽  
The Way

The object of the study of the knowledge of the variety of the Quranic reading  is the  Qur'an itself. The focus is on the difference of the reading and its articulation. The method is based on the riwayat or narration which is originated from the Prophet (Rasulullah saw) and its use is to be one of the instruments to keep the originality of the Qur’an. The validity of the reading the Qur’an is to be judged based on the valid chain  (sanad ¡a¥ī¥)  in accord with the Rasm U£mānÄ« as well as with the  Arabic grammar. Whereas the qualification of its originality is divided into six stages as follow: the first is mutawātir, the second is masyhÅ«r, the third is āhād, the fourth is syaz, the fifth is maudū‘, and the six is mudraj. Of this six catagories, the readings which can be included in the catagory of mutawātir are Qiraat Sab‘ah (the seven readings) and Qiraat ‘Asyrah  (the ten readings). To study this knowledge of reading the Qur’an (ilmu qiraat), one is advised to know about special terms being used such as  qiraat  (readings), riwayat (narration), tarÄ«q (the way), wajh (aspect), mÄ«m jama‘, sukÅ«n mÄ«m jama‘ and many others.

Causation, Explanation, and the Metaphysics of Aspect

2018 ◽  
Author(s):  
Bradford Skow
Keyword(s):  
Background Condition ◽  
Structural Explanation ◽  
Levels Of Explanation ◽  
Lexical Aspect ◽  
The Third ◽  
Main Verb ◽  
The Difference ◽  
In Virtue Of

This book aims to answer the following questions: what is the difference between a cause and a background condition? What is it to manifest a disposition? Can dispositions be extrinsic? What is the most basic kind of causation? And, what might a structural explanation be? Each chapter takes up a subset of these questions; the chapters are written to be readable independently. The answers defended rely on three ideas. Two of those ideas use a distinction from the study of lexical aspect, namely the distinction between stative verbs and non-stative verbs. The first idea is that events go with non-stative verbs, in the sense that “If S, then an event occurred in virtue of the fact that S” is true when the main verb in the clause going in for “S” is non-stative. The second is that acting, doing something, goes with non-stative verbs, in the sense that “In Ving X did something” is true iff V is a non-stative verb. The third idea is about levels of explanation: “(A because B) because C” does not entail “A because C.”

Three Perspectives on Centrality

2020 ◽  
pp. 333-351
Author(s):  
Stephen P. Borgatti ◽  
Martin G. Everett
Keyword(s):  
Centrality Measures ◽  
Graph Invariant ◽  
Propagation Process ◽  
Centrality Measure ◽  
The Third ◽  
First Arrival ◽  
The Difference

This chapter presents three different perspectives on centrality. In part, the motivation is definitional: what counts as a centrality measure and what doesn’t? But the primary purpose is to lay out ways that centrality measures are similar and dissimilar and point to appropriate ways of interpreting different measures. The first perspective the chapter considers is the “walk structure participation” perspective. In this perspective, centrality measures indicate the extent and manner in which a node participates in the walk structure of a graph. A typology is presented that distinguishes measures based on dimensions such as (1) what kinds of walks are considered (e.g., geodesics, paths, trails, or unrestricted walks) and (2) whether the number of walks is counted or the length of walks is assessed, or both. The second perspective the chapter presents is the “induced centrality” perspective, which views a node’s centrality as its contribution to a specific graph invariant—typically some measure of the cohesiveness of the network. Induced centralities are computed by calculating the graph invariant, removing the node in question, and recalculating the graph invariant. The difference is the node’s centrality. The third perspective is the “flow outcomes” perspective. Here the chapter views centralities as estimators of node outcomes in some kind of propagation process. Generic node outcomes include how often a bit of something propagating passes through a node and the time until first arrival of something flowing. The latter perspective leads us to consider the merits of developing custom measures for different research settings versus using off-the-shelf measures that were not necessarily designed for the current purpose.

scholarly journals Increased risk of preeclampsia after use of paracetamol during pregnancy – causal or coincidence?

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hetti von Hellens ◽  
Leea Keski-Nisula ◽  
Heidi Sahlman
Keyword(s):  
Third Trimester ◽  
Retrospective Case ◽  
Reverse Causation ◽  
Gestational Period ◽  
The Third ◽  
Precise Data ◽  
Increased Risk ◽  
Study Population ◽  
The Difference ◽  
Risk Of Preeclampsia

Abstract Background The maternal use of paracetamol during pregnancy has been associated with the development of preeclampsia. This study aims to clarify whether the connection is causal or whether it is due to reverse causation. Methods This study is a continuation of the retrospective case cohort study examining 2,508 pregnant women using a variety of drugs and the development of preeclampsia (1,252 women with preeclampsia and 1,256 controls). For the purposes of this study, more precise data was collected from several hospital databases of the women among this cohort who had reported taking paracetamol during pregnancy (indications, gestational period etc.); this was evaluated in association with the development of preeclampsia. Results 5.5% (100 cases and 37 controls) of all the study population (2,508) had clearly reported paracetamol use. Women with preeclampsia had used significantly more often paracetamol during pregnancy compared to controls (cases 8.0%, controls 2.9%, p < 0.001). The difference was most evident in the third trimester (after the 29th GW) and the use of paracetamol was associated with both mild and severe preeclampsia. Headache and “general pain” were the most common indications for medication among all paracetamol users. Conclusions The use of paracetamol in the third trimester of pregnancy was associated with preeclampsia. This observation indicates that association between paracetamol use and preeclampsia is probably due to reverse causation, i.e. women with preeclampsia experience more headaches due to preeclampsia symptoms since this association was not detected with the use of paracetamol in earlier stages of pregnancy.

scholarly journals Apicomplexan Protozoa Responsible for Reproductive Disorders: Occurrence of DNA in Blood and Milk of Donkeys (Equus asinus) and Minireview of the Related Literature

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 111
Author(s):  
Stefania Perrucci ◽  
Lisa Guardone ◽  
Iolanda Altomonte ◽  
Federica Salari ◽  
Simona Nardoni ◽  
...  
Keyword(s):  
Animal Species ◽  
Neospora Caninum ◽  
Reproductive Disorders ◽  
Theileria Equi ◽  
Blood Samples ◽  
Related Literature ◽  
Milk Samples ◽  
Equus Asinus ◽  
Time Periods ◽  
Blood Specimens

Donkeys may be susceptible to many pathological agents and may act as carriers of pathogens for other animal species and humans. This study evaluated the occurrence of potentially abortifacient apicomplexan protozoa DNA in blood and milk samples collected at different time periods during lactation (1, 6, and 10 months) from 33 healthy dairy jennies. A total of 73 blood and 73 milk samples were used for DNA extraction and analysis. Blood specimens from 11/33 (33%) jennies scored positive for Theileria equi, while milk samples scored negative. Blood and milk of 3/33 jennies yielded DNA of Toxoplasma gondii at 6 months (n. 1) and 10 months (n. 2) after parturition. Neospora caninum DNA was found in four milk and in five blood samples only at one month after parturition. This study is the first report about the presence of N. caninum DNA in milk of naturally infected jennies. Moreover, the excretion of N. caninum DNA in some of these jennies at 30 days from the parturition may suggest a possible occurrence of an endogenous cycle, while the presence of T. gondii DNA in the milk collected at 6 and 10 months after parturition may be suggestive of a discontinuous excretion.

scholarly journals How to talk with the family of a dying patient: anger to understanding, rage to compassion, loss to acceptance

2021 ◽  
pp. bmjspcare-2021-002971
Author(s):  
Moshe Y Flugelman
Keyword(s):  
Goal Setting ◽  
Stressful Situation ◽  
Family Needs ◽  
The Third ◽  
Single Entity ◽  
The Family ◽  
Patient Families ◽  
The Difference ◽  
Dying Patient

Informing families about the impending or actual death of their relatives is one of the most challenging and complex tasks a physician may face. The following article describes goal setting and provides five roles/recommendations for conducting the encounter with patient families regarding the imminent or actual death of their relatives. Importantly, the encounter should be family-centred, and the physician should be highly attentive to family needs. The following roles should be applied based on family needs and should not be sequential as numbered. The first and basic role is to inform the family at the earliest possible time and as often as possible. The second goal of the physician is to convey to the family that their relative received the needed therapy during his hospitalisation or in the community. The third goal of the physician is to help the family reach acceptance of the death of their relative and leave the hospital having moved beyond anger and bargaining. The fourth goal of the physician during the encounters is to reduce or alleviate guilt by stating that nothing could have changed the course of the disease and that all efforts were made to save the patient. The fifth role of the physician is to try and help the family as a single entity and maintain their unity during this stressful situation. Following these roles/methods will help families in the stressful situation and will create the difference between anger and understanding, rage and compassion, and loss and acceptance.

Sign in / Sign up

Export Citation Format

Share Document