Light and scanning electron microscopy of attachment organs of three monogeneans (Monogenoidea: Polyopisthocotylea)

1974 ◽  
Vol 52 (2) ◽  
pp. 183-187 ◽  
Author(s):  
K. A. Wright ◽  
A. Dechtiar
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Light Microscope ◽  
The Body ◽  
Single Pair ◽  
The Third ◽  
Gland Cells ◽  
Whole Mount ◽  
Scanning Electron

The anterior and posterior attachment organs of three species of monogeneans, Diclybothrium armatum Leuckart, 1835, Neodiscocotyle carpioditis Dechtiar, 1967, and Mazocraeoides olentangiensis Sroufe, 1958, were examined in whole-mount light-microscope preparations, and by scanning electron microscopy (SEM). Bothria with openings of gland cells were identified anterior to the oral opening in D. armatum, but sucker-like units evident in light microscopy were not seen by SEM in N. carpioditis and M. olentangiensis as these are apparently internal developments. Holdfast units of the opisthaptor of the three species are of three distinct forms: spring-like clamps in D. armatum, muscular clamps in M. olentangiensis. and suckers with sclerotized rims in N. carpioditis. Hooks in the opisthaptor appendage of D. armatum all function to form a C-shaped double-pronged hook unit. Two of the three pairs of hooks in the posterior end of M. olentangiensis project slightly from the body, while the third pair in this species and the single pair of hooks in N. carpioditis are entirely internal, probably non-functional and vestigial.

Comparison of Larval Stored Product Beetle Mandibles by Light Microscope and Scanning Electron Microscope

1981 ◽  
Vol 64 (1) ◽  
pp. 199-224
Author(s):  
John E Kvenberg
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Scanning Electron ◽  
Conventional Light Microscopy

Abstract Larval stored product beetle mandibles were studied by comparing images made by scanning electron microscopy with those made by conventional light microscopy. Discussion of morphological characteristics is based on illustrations of 25 species

Description of the newly-hatched juvenile of Aegla castro Schmitt, 1942 (Crustacea, Anomura, Aeglidae)

Zootaxa ◽  
2017 ◽  
Vol 4237 (1) ◽  
pp. 167 ◽  
Author(s):  
LUANE SAMARA ALVES E SILVA ◽  
CECILIA MARGARITA GUERRERO-OCAMPO ◽  
MARIA LUCIA NEGREIROS-FRANSOZO ◽  
GUSTAVO MONTEIRO TEIXEIRA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Water Temperature ◽  
Juvenile Stage ◽  
The Third ◽  
Asynchronous Hatching ◽  
Scanning Electron

This study describes and illustrates the morphology of the first juvenile stage of Aegla castro Schmitt, 1942. Ovigerous females were collected from May to July 2013, in Couro River (Mauá da Serra, Paraná, Brazil). These females were kept individually under controlled feeding, aeration, water temperature and quality and checked daily for hatching of juveniles. The newly-hatched juveniles were fixed in alcohol series and kept in 70% alcohol with glycerin in a 2:1 ratio prior to study under light microscopy. The newly-hatched juvenile of A. castro is the largest among aeglid species whose juveniles have been described. Aegla castro has asynchronous hatching. Some specimens were analyzed using scanning electron microscopy, revealing details of the setal morphology, some cephalothoracic appendages and lineae aeglicae. The number of setae in newly-hatched A. castro is lower than that described for other species, but does not appear to be diagnostic. However, A. castro is the only species described that combines the presence of four plumose setae on the third maxilliped exopod and 63–65 plumose setae on the maxilla exopod. 

Microscopic study of Forcipomyia whitcombe ovary

1994 ◽  
Vol 52 ◽  
pp. 360-361
Author(s):  
Taher A. Ba-Omar ◽  
David Devera
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Critical Point ◽  
Sperm Storage ◽  
The Body ◽  
Sultanate Of Oman ◽  
Follicular Cells ◽  
Biting Midges ◽  
Scanning Electron

We have been investigating the structure of the Forcipomyia whitcombe (Diplera: Ceratopogonidae) ovary. The F. Whitcombe are biting midges which are one of the newly discovered species of Diptera: Ceratopogonidae found in Oman. They are found in bushland and grassland of the mountain of Dhofar (southern part of the Sultanate of Oman); Jabal Oara and Jabal Qamar. These midges are associated with the monsoon which hits that part of Oman during the summer months (end of June - mid September).Specimens were collecled using nets and fix directly in 70% alcohol. Some specimens were processed for light microscopy (LM) using routine histological technique and stained with H&E. Others were processed for scanning electron microscopy (SEM); dehydrated in acetone, dried in a critical point dryer (CPD). They were then cut into halves in which the lower half was coaled with gold and then examined with Jeol SEM.The body of the insect measures about 1.3 mm in length. The study showed that this insect possesses paired ovaries. Next to the ovaries there is the spermatheca (sperm storage). Each ovary contains several ovarioles polytrophic type. Each ovariole possesses several eggs which are enclosed in a layer of follicular cells (supporting and nourishing cells).

Light and scanning electron microscopic studies on Trichodina labrisomi n.sp. from Labrisomas nuchipinnis (Osteichthyes: Labrisomidae) from Mangrove Lake, Bermuda

1993 ◽  
Vol 71 (9) ◽  
pp. 1855-1860
Author(s):  
Thomas G. Rand
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Body Shape ◽  
The Body ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Microscopic Studies ◽  
Oral Surface ◽  
Scanning Electron

Trichodina labrisomi n.sp. from the gills of the hairy blenny, Labrisomas nuchipinnis (Quoy and Gaimard), in Mangrove Lake, Bermuda, is described using light microscopy and scanning electron microscopy (SEM). Light microscopy reveals that the dimensions and morphology of T. labrisomi are similar to those given for only 13 previously described species of Trichodina (T. acuta Lom, 1961; T. baicalensis Dogiel, 1957; T. cottomephori Stein, 1979; T. dalli Zhukov, 1964; Trichodina domerguei domerguei Lom and Shtein 1966; T. elegans Stein, 1979; T. fultoni Davis, 1947; T. jadranica Haider, 1964; T. jadranica noblei Lom, 1970; T. partidisci Haider, 1964; T. reticulata Hirschmann et Partsch, 1955; T. tenuidens Faure-Fremiet, 1943; and T. tenuiformis Stein, 1979). However, compared with these similar forms, T. labrisomi n.sp. is distinguished by the dimensions of its body and denticulate ring and (or) the appearance of its silver-impregnated adhesive disc and denticles. Features of T. labrisomi examined by SEM include the body shape, pellicle, aboral pellicular pores, border membrane, aboral ciliary complex, and adoral ciliature, which are described and compared with those of other trichodinids studied using SEM. SEM also revealed that bacilli were distributed circumequatorially over the oral surface of T. labrisomi.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Examination of Schistosome Cercariae and Schistosomules by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 50-51
Author(s):  
D. Johnson ◽  
P. Moriearty
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Light Microscopy ◽  
Surface Structure ◽  
Extensive Study ◽  
Scanning Microscopy ◽  
Host Parasite ◽  
Conventional Electron Microscopy ◽  
Scanning Electron

Since several species of Schistosoma, or blood fluke, parasitize man, these trematodes have been subjected to extensive study. Light microscopy and conventional electron microscopy have yielded much information about the morphology of the various stages; however, scanning electron microscopy has been little utilized for this purpose. As the figures demonstrate, scanning microscopy is particularly helpful in studying at high resolution characteristics of surface structure, which are important in determining host-parasite relationships.

SEM Observations on the Germination of Euonymous Americanus

1985 ◽  
Vol 43 ◽  
pp. 508-509
Author(s):  
D.R. Hill ◽  
J.R. McCurry ◽  
L.P. Elliott ◽  
G. Howard
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Filter Paper ◽  
Room Temperature ◽  
Food Source ◽  
Environmental Chamber ◽  
Dormant Stage ◽  
Scanning Electron

Germination of Euonymous americanus in the laboratory has previously been unsuccessful. Ability to germinate Euonymous americanus. commonly known as the american strawberry bush, is important in that it represents a valuable food source for the white-tailed deer. Utilizing the knowledge that its seeds spend a period of time in the rumin fluid of deer during their dormant stage, we were successful in initiating germination. After a three month drying period, the seeds were placed in 25 ml of buffered rumin fluid, pH 8 at 40°C for 48 hrs anaerobically. They were then allowed to dry at room temperature for 24 hrs, placed on moistened filter paper and enclosed within an environmental chamber. Approximately four weeks later germination was detected and verified by scanning electron microscopy; light microscopy provided inadequate resolution. An important point to note in this procedure is that scarification, which was thought to be vital for germination, proved to be unnecessary for successful germination to occur. It is believed that germination was propagated by the secretion of enzymes or prescence of acids produced by microorganisms found in the rumin fluid since sterilized rumin failed to bring about germination.

New diagnostic characters of Kolhymorbis angarensis (Dybowski & Grochmalicki, 1925) (Gastropoda: Pulmonata: Planorbidae)

2009 ◽  
Vol 18 (2) ◽  
pp. 191-195
Author(s):  
E.V. Soldatenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Diagnostic Characters ◽  
Copulatory Apparatus ◽  
Scanning Electron

The radula morphology and the anatomy of the copulatory apparatus in Kolhymorbis angarensis were examined using light microscopy, scanning electron microscopy (SEM) and histological methods. Kolhymorbis angarensis was shown to have the stylet and the penial sac with a glandular appendage (flagellum), the characteristics, previously unknown for any species of this genus. The significance of these findings for the taxonomy of the genus is discussed.

Preparation of Alcohol-Preserved Larvae of Culicidae (Diptera) for Scanning Electron Microscopy

1972 ◽  
Vol 3 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Christine Dahl
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Body ◽  
Taxonomic Studies ◽  
Scanning Electron ◽  
Sem Studies

AbstractA method for preparation of alcohol-preserved culicid larvae for Scanning Electron Microscope (SEM) studies is described. It is based on dehydration by ethanol-xylol and fast evaporation of xylol in +8o° C. for ten minutes. For taxonomic studies such as examination of pecten teeth, comb scales and microtrichiae in magnifications up to 6oooX the method is suitable. For studies of receptor structures on hair-tufts and microstructures of the body integument alcohol preserved material is less satisfactory. The microstructure of the comb scales is figured and their function discussed. Differences in the ultrastructure of the abdominal hair-tufts are pointed out.

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