System design and new applications for atomic force microscope based on tunneling

2015 ◽  
Vol 29 (25n26) ◽  
pp. 1542039
Author(s):  
X. Wang ◽  
A. P. Liu ◽  
X. H. Yang
Keyword(s):  
System Design ◽  
Protein A ◽  
Protein Structures ◽  
Human Antibody ◽  
Force Microscopy ◽  
Atomic Force ◽  
Measurement Results ◽  
Original Frame ◽  
New Applications ◽  
Tunnel Microscopy

The design of atomic force microscopy (AFM) with high resolution is introduced in this paper. Mainly, we have developed the system design of the apparatus based on tunneling. AFM.IPC-208B, this kind of apparatus combines scanning tunnel microscopy (STM) and AFM availability, and its lens body with original frame enhances the capability of the machine. In order to analyze the performance of AFM.IPC-208B, as a new tool in the field of Life Science, we make use of the system to study natural mica and molecular protein structures of Cattle-insulin and human antibody immunoglobulin G (IgG) coupled with staphylococcus protein A (SPA). As the results of new applications, the resolution of AFM.IPC-208B is proved to be 0.1 nm, and these nanometer measurement results provide much valuable information for the study of small molecular proteins and HIV experiments.

scholarly journals Dynamic friction energy dissipation and enhanced contrast in high frequency bimodal atomic force microscopy

Friction ◽  
2021 ◽  
Author(s):  
Xinfeng Tan ◽  
Dan Guo ◽  
Jianbin Luo
Keyword(s):  
Atomic Force Microscopy ◽  
Energy Dissipation ◽  
Contact Friction ◽  
Measuring Instruments ◽  
Dynamic Friction ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Friction Energy ◽  
New Applications

AbstractDynamic friction occurs not only between two contact objects sliding against each other, but also between two relative sliding surfaces several nanometres apart. Many emerging micro- and nano-mechanical systems that promise new applications in sensors or information technology may suffer or benefit from noncontact friction. Herein we demonstrate the distance-dependent friction energy dissipation between the tip and the heterogeneous polymers by the bimodal atomic force microscopy (AFM) method driving the second order flexural and the first order torsional vibration simultaneously. The pull-in problem caused by the attractive force is avoided, and the friction dissipation can be imaged near the surface. The friction dissipation coefficient concept is proposed and three different contact states are determined from phase and energy dissipation curves. Image contrast is enhanced in the intermediate setpoint region. The work offers an effective method for directly detecting the friction dissipation and high resolution images, which overcomes the disadvantages of existing methods such as contact mode AFM or other contact friction and wear measuring instruments.

scholarly journals Atomic force microscopy recognition of protein A onStaphylococcus aureuscell surfaces by labelling with IgG–Au conjugates

2013 ◽  
Vol 4 ◽  
pp. 743-749 ◽  
Author(s):  
Elena B Tatlybaeva ◽  
Hike N Nikiyan ◽  
Alexey S Vasilchenko ◽  
Dmitri G Deryabin
Keyword(s):  
Atomic Force Microscopy ◽  
Protein A ◽  
Bacterial Cells ◽  
Force Microscopy ◽  
Selective Labelling ◽  
Atomic Force ◽  
Novel Approach ◽  
Preferential Binding ◽  
Igg Receptors ◽  
Labelling Method

The labelling of functional molecules on the surface of bacterial cells is one way to recognize the bacteria. In this work, we have developed a method for the selective labelling of protein A on the cell surfaces ofStaphylococcus aureusby using nanosized immunogold conjugates as cell-surface markers for atomic force microscopy (AFM). The use of 30-nm size Au nanoparticles conjugated with immunoglobulin G (IgG) allowed the visualization, localization and distribution of protein A–IgG complexes on the surface ofS. aureus. The selectivity of the labelling method was confirmed in mixtures ofS. aureuswithBacillus licheniformiscells, which differed by size and shape and had no IgG receptors on the surface. A preferential binding of the IgG–Au conjugates toS. aureuswas obtained. Thus, this novel approach allows the identification of protein A and other IgG receptor-bearing bacteria, which is useful for AFM indication of pathogenic microorganisms in poly-component associations.

An Operating Method with Lateral Scan for Reducing the Error in Topography Caused by the Tip-Sample Angle in Atomic Force Microscopy

2010 ◽  
Vol 16 (5) ◽  
pp. 630-635
Author(s):  
Fa-Quan Zhou ◽  
Xue-Zeng Zhao ◽  
Fei Wang ◽  
Yue-Yu Wang
Keyword(s):  
Atomic Force Microscopy ◽  
Topographic Image ◽  
Contact Mode ◽  
Atomic Force Microscopes ◽  
Force Microscopy ◽  
Atomic Force ◽  
Measurement Results ◽  
Parallel Scan ◽  
Operating Method

AbstractThe atomic force microscopes (AFM) images are obtained by keeping the bending of the cantilever unchanged in contact mode. However, it is found that changes in the tip-sample angle during parallel scan result in error in the topographic image. It is also discovered that measurement results obtained in the blind scan region contained large errors. In contrast, regions opposite the blind scan region gave more reliable result. To eliminate this topographic error caused by change in the tip-sample angle, a new operating method with lateral scan is utilized in AFM. Comparative experiments have been performed, and the results show that the error could be eliminated or decreased by using the operating method.

scholarly journals Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy

2005 ◽  
Vol 71 (2) ◽  
pp. 955-960 ◽  
Author(s):  
Liming Zhao ◽  
David Schaefer ◽  
Mark R. Marten
Keyword(s):  
Atomic Force Microscopy ◽  
Aspergillus Nidulans ◽  
Fungal Spores ◽  
Protein Structures ◽  
Spore Surface ◽  
Wild Type ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm

ABSTRACT Previous studies have described both surface morphology and adhesive properties of fungal spores, but little information is currently available on their mechanical properties. In this study, atomic force microscopy (AFM) was used to investigate both surface topography and micromechanical properties of Aspergillus nidulans spores. To assess the influence of proteins covering the spore surface, wild-type spores were compared with spores from isogenic rodA + and rodA − strains. Tapping-mode AFM images of wild-type and rodA + spores in air showed characteristic “rodlet” protein structures covering a granular spore surface. In comparison, rodA − spores were rodlet free but showed a granular surface structure similar to that of the wild-type and rodA + spores. Rodlets were removed from rodA + spores by sonication, uncovering the underlying granular layer. Both rodlet-covered and rodlet-free spores were subjected to nanoindentation measurements, conducted in air, which showed the stiffnesses to be 110 ± 10, 120 ± 10, and 300 ± 20 N/m and the elastic moduli to be 6.6 ± 0.4, 7.0 ± 0.7, and 22 ± 2 GPa for wild-type, rodA + and rodA − spores, respectively. These results imply the rodlet layer is significantly softer than the underlying portion of the cell wall.

The investigation of protein A andSalmonella antibody adsorption onto biosensor surfaces by atomic force microscopy

2008 ◽  
Vol 99 (4) ◽  
pp. 949-959 ◽  
Author(s):  
Kyoung G. Lee ◽  
Shreekumar R. Pillai ◽  
Shree R. Singh ◽  
Gerold A. Willing
Keyword(s):  
Atomic Force Microscopy ◽  
Protein A ◽  
Force Microscopy ◽  
Atomic Force

PEPTIDE NANOFILAMENTS USED FOR REPLICA-MOLDING: A COMBINATION OF "BOTTOM-UP" AND "TOP-DOWN"

2007 ◽  
Vol 14 (02) ◽  
pp. 301-307 ◽  
Author(s):  
YUZHAO TANG ◽  
XIAODONG ZHANG ◽  
JIELIN SUN ◽  
JUN HU ◽  
FENG ZHANG ◽  
...  
Keyword(s):  
Nanometer Scale ◽  
Top Down ◽  
Bottom Up ◽  
Polymeric Surfaces ◽  
Replica Molding ◽  
Force Microscopy ◽  
Self Assembling ◽  
Atomic Force ◽  
New Applications

A novel nanofabrication method that combines both "bottom-up" (template-assisted peptide self-assembling) and "top-down" (replica molding) techniques is introduced. A designer peptide, GAV-9 (NH2-VGGAVVAGV-CONH2), can epitaxially self-assemble into nanofilaments on the surface of mica, which is further used as the diversified masters for the application of replica molding. With in situ atomic force microscopy monitoring, several typical masters are fabricated by peptide self-assembling on the surface of mica. These masters can be easily molded into hard poly(dimethylsiloxane) surfaces, and then further replica-molded into polyurethane surfaces. The polymeric surfaces with regular 1D and 2D patterns on the nanometer scale are expected to have new applications in nanostructure's fabrication.

scholarly journals The Effect of Ethanol on Disassembly of Amyloid-β1-42 Pentamer Revealed by Atomic Force Microscopy and Gel Electrophoresis

2022 ◽  
Vol 23 (2) ◽  
pp. 889
Author(s):  
Atsuya Matsui ◽  
Jean-Pierre Bellier ◽  
Takeshi Kanai ◽  
Hiroki Satooka ◽  
Akio Nakanishi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Quantitative Estimation ◽  
Senile Plaques ◽  
Protein Structures ◽  
Amyloid Β ◽  
Ambient Air ◽  
Native Page ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Impact

The most common type of dementia, Alzheimer’s disease, is associated with senile plaques formed by the filamentous aggregation of hydrophobic amyloid-β (Aβ) in the brains of patients. Small oligomeric assemblies also occur and drugs and chemical compounds that can interact with such assemblies have attracted much attention. However, these compounds need to be solubilized in appropriate solvents, such as ethanol, which may also destabilize their protein structures. As the impact of ethanol on oligomeric Aβ assembly is unknown, we investigated the effect of various concentrations of ethanol (0 to 7.2 M) on Aβ pentameric assemblies (Aβp) by combining blue native-PAGE (BN-PAGE) and ambient air atomic force microscopy (AFM). This approach was proven to be very convenient and reliable for the quantitative analysis of Aβ assembly. The Gaussian analysis of the height histogram obtained from the AFM images was correlated with band intensity on BN-PAGE for the quantitative estimation of Aβp. Our observations indicated up to 1.4 M (8.3%) of added ethanol can be used as a solvent/vehicle without quantitatively affecting Aβ pentamer stability. Higher concentration induced significant destabilization of Aβp and eventually resulted in the complete disassembly of Aβp.

Recent Progress in Self-sensing Probe Technology in Atomic Force Microscope

2021 ◽  
Vol 17 ◽  
Author(s):  
Ke Xu ◽  
Chenghao Ji ◽  
Fanan Wei ◽  
Zhushan Zhai
Keyword(s):  
Atomic Force Microscope ◽  
The Self ◽  
The Novel ◽  
Reflection Method ◽  
Force Microscopy ◽  
Atomic Force ◽  
Sample Characterization ◽  
Cantilever Array ◽  
New Applications ◽  
Imaging Performance

: The imaging speed of atomic force microscope (AFM) is limited by the cantilever mechanical bandwidth, which can be increased by reducing the size of the cantilever. However, the ordinary laser reflection method cannot measure the deflection of a small cantilever. And some samples are sensitive to light detection and not suitable for the laser reflection method. Therefore, the self-induction probe technology was developed to solve this problem. This article reviews the latest AFM self-induction probe technology and introduces three types of self-induction probes. Firstly, it is introduced that the current self-sensing probes can be divided into piezoresistive type, piezoelectric type and tuning fork type according to the working mechanisms and preparation materials. Then, the latest materials and structures of various self-sensing probe technologies are introduced to improve imaging performance and their applications in various fields. And, compared with traditional laser reflection methods, the self-sensing probe technologies have a simpler structure, take up less space and can be integrated in a large cantilever array and adopted for imaging of photosensitive products. Finally, some prospects of the novel imaging and sample characterization techniques and new applications of atomic force microscopy are also discussed.

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