scholarly journals Spatial-spectral identification of abnormal leukocytes based on microscopic hyperspectral imaging technology

2020 ◽  
Vol 13 (02) ◽  
pp. 2050005
Author(s):  
Xueqi Hu ◽  
Jiahua Ou ◽  
Mei Zhou ◽  
Menghan Hu ◽  
Li Sun ◽  
...  
Keyword(s):  
Hyperspectral Imaging ◽  
Lymphoblastic Leukemia ◽  
Spectral Feature ◽  
Support Vector ◽  
Watershed Algorithm ◽  
Spectral Features ◽  
Accurate Identification ◽  
Spatial Features ◽  
Spectral Identification

Screening and diagnosing of abnormal Leukocytes are crucial for the diagnosis of immune diseases and Acute Lymphoblastic Leukemia (ALL). As the deterioration of abnormal leukocytes is mainly due to the changes in the chromatin distribution, which significantly affects the absorption and reflection of light, the spectral feature is proved to be important for leukocytes classification and identification. This paper proposes an accurate identification method for healthy and abnormal leukocytes based on microscopic hyperspectral imaging (HSI) technology which combines the spectral information. The segmentation of nucleus and cytoplasm is obtained by the morphological watershed algorithm. Then, the spectral features are extracted and combined with the spatial features. Based on this, the support vector machine (SVM) is applied for classification of five types of leukocytes and abnormal leukocytes. Compared with different classification methods, the proposed method utilizes spectral features which highlight the differences between healthy leukocytes and abnormal leukocytes, improving the accuracy in the classification and identification of leukocytes. This paper only selects one subtype of ALL for test, and the proposed method can be applied for detection of other leukemia in the future.

scholarly journals Rapid Classification of Wheat Grain Varieties Using Hyperspectral Imaging and Chemometrics

2019 ◽  
Vol 9 (19) ◽  
pp. 4119 ◽  
Author(s):  
Yidan Bao ◽  
Chunxiao Mi ◽  
Na Wu ◽  
Fei Liu ◽  
Yong He
Keyword(s):  
Hyperspectral Imaging ◽  
Large Scale ◽  
Scatter Correction ◽  
Principal Component ◽  
Support Vector ◽  
Wheat Grain ◽  
Successive Projections Algorithm ◽  
Accurate Identification ◽  
Wheat Varieties

The classification of wheat grain varieties is of great value because its high purity is the yield and quality guarantee. In this study, hyperspectral imaging combined with the chemometric methods was applied to explore and implement the varieties classification of wheat seeds. The hyperspectral images of all the samples covering 874–1734 nm bands were collected. Exploratory analysis was first carried out while using principal component analysis (PCA) and linear discrimination analysis (LDA). Spectral preprocessing methods including standard normal variate (SNV), multiplicative scatter correction (MSC), and wavelet transform (WT) were introduced, and their effects on discriminant models were studied to eliminate the interference of instrumental and environmental factors. PCA loading, successive projections algorithm (SPA), and random frog (RF) were applied to extract feature wavelengths for redundancy elimination owing to the possibility of existing redundant spectral information. Classification models were developed based on full wavelengths and feature wavelengths using LDA, support vector machine (SVM), and extreme learning machine (ELM). This optimal model was finally utilized to generate visualization map to observe the classification performance intuitively. When comparing with other models, ELM based on full wavelengths achieved the best accuracy up to 91.3%. The overall results suggested that hyperspectral imaging was a potential tool for the rapid and accurate identification of wheat varieties, which could be conducted in large-scale seeds classification and quality detection in modern seed industry.

scholarly journals Classification of complex emotions using EEG and virtual environment: proof of concept and therapeutic implication

2020 ◽  
Author(s):  
Eleonora De Filippi ◽  
Mara Wolter ◽  
Bruno Melo ◽  
Carlos J. Tierra-Criollo ◽  
Tiago Bortolini ◽  
...  
Keyword(s):  
Real Time ◽  
Cross Validation ◽  
Support Vector ◽  
Spectral Features ◽  
Emotional States ◽  
Proof Of Concept ◽  
Neurofeedback Training ◽  
Concept Study ◽  
Complex Emotions

AbstractDuring the last decades, neurofeedback training for emotional self-regulation has received significant attention from both the scientific and clinical communities. However, most studies have focused on broader emotional states such as “negative vs. positive”, primarily due to our poor understanding of the functional anatomy of more complex emotions at the electrophysiological level. Our proof-of-concept study aims at investigating the feasibility of classifying two complex emotions that have been implicated in mental health, namely tenderness and anguish, using features extracted from the electroencephalogram (EEG) signal in healthy participants. Electrophysiological data were recorded from fourteen participants during a block-designed experiment consisting of emotional self-induction trials combined with a multimodal virtual scenario. For the within-subject classification, the linear Support Vector Machine was trained with two sets of samples: random cross-validation of the sliding windows of all trials; and 2) strategic cross-validation, assigning all the windows of one trial to the same fold. Spectral features, together with the frontal-alpha asymmetry, were extracted using Complex Morlet Wavelet analysis. Classification results with these features showed an accuracy of 79.3% on average when doing random cross-validation, and 73.3% when applying strategic cross-validation. We extracted a second set of features from the amplitude time-series correlation analysis, which significantly enhanced random cross-validation accuracy while showing similar performance to spectral features when doing strategic cross-validation. These results suggest that complex emotions show distinct electrophysiological correlates, which paves the way for future EEG-based, real-time neurofeedback training of complex emotional states.Significance statementThere is still little understanding about the correlates of high-order emotions (i.e., anguish and tenderness) in the physiological signals recorded with the EEG. Most studies have investigated emotions using functional magnetic resonance imaging (fMRI), including the real-time application in neurofeedback training. However, concerning the therapeutic application, EEG is a more suitable tool with regards to costs and practicability. Therefore, our proof-of-concept study aims at establishing a method for classifying complex emotions that can be later used for EEG-based neurofeedback on emotion regulation. We recorded EEG signals during a multimodal, near-immersive emotion-elicitation experiment. Results demonstrate that intraindividual classification of discrete emotions with features extracted from the EEG is feasible and may be implemented in real-time to enable neurofeedback.

scholarly journals Comparison and Combination of Thermal, Fluorescence, and Hyperspectral Imaging for Monitoring Fusarium Head Blight of Wheat on Spikelet Scale

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2281 ◽  
Author(s):  
Anne-Katrin Mahlein ◽  
Elias Alisaac ◽  
Ali Al Masri ◽  
Jan Behmann ◽  
Heinz-Wilhelm Dehne ◽  
...  
Keyword(s):  
Fusarium Head Blight ◽  
Hyperspectral Imaging ◽  
Optical Sensors ◽  
Fusarium Culmorum ◽  
Maximum Temperature ◽  
Support Vector ◽  
Head Blight ◽  
Maximum Temperature Difference ◽  
Simple Ratio

Optical sensors have shown high capabilities to improve the detection and monitoring of plant disease development. This study was designed to compare the feasibility of different sensors to characterize Fusarium head blight (FHB) caused by Fusarium graminearum and Fusarium culmorum. Under controlled conditions, time-series measurements were performed with infrared thermography (IRT), chlorophyll fluorescence imaging (CFI), and hyperspectral imaging (HSI) starting 3 days after inoculation (dai). IRT allowed the visualization of temperature differences within the infected spikelets beginning 5 dai. At the same time, a disorder of the photosynthetic activity was confirmed by CFI via maximal fluorescence yields of spikelets (Fm) 5 dai. Pigment-specific simple ratio PSSRa and PSSRb derived from HSI allowed discrimination between Fusarium-infected and non-inoculated spikelets 3 dai. This effect on assimilation started earlier and was more pronounced with F. graminearum. Except the maximum temperature difference (MTD), all parameters derived from different sensors were significantly correlated with each other and with disease severity (DS). A support vector machine (SVM) classification of parameters derived from IRT, CFI, or HSI allowed the differentiation between non-inoculated and infected spikelets 3 dai with an accuracy of 78, 56 and 78%, respectively. Combining the IRT-HSI or CFI-HSI parameters improved the accuracy to 89% 30 dai.

scholarly journals Classification of Rice and Starch Flours by Using Multiple Hyperspectral Imaging Systems and Chemometric Methods

2020 ◽  
Vol 10 (19) ◽  
pp. 6724
Author(s):  
Youngwook Seo ◽  
Ahyeong Lee ◽  
Balgeum Kim ◽  
Jongguk Lim
Keyword(s):  
Discriminant Analysis ◽  
Hyperspectral Imaging ◽  
Partial Least Square ◽  
Hyperspectral Images ◽  
Classification Model ◽  
Support Vector ◽  
Chemometric Methods ◽  
Infrared Wavelength ◽  
Linear Discriminant

(1) Background: The general use of food-processing facilities in the agro-food industry has increased the risk of unexpected material contamination. For instance, grain flours have similar colors and shapes, making their detection and isolation from each other difficult. Therefore, this study is aimed at verifying the feasibility of detecting and isolating grain flours by using hyperspectral imaging technology and developing a classification model of grain flours. (2) Methods: Multiple hyperspectral images were acquired through line scanning methods from reflectance of visible and near-infrared wavelength (400–1000 nm), reflectance of shortwave infrared wavelength (900–1700 nm), and fluorescence (400–700 nm) by 365 nm ultraviolet (UV) excitation. Eight varieties of grain flours were prepared (rice: 4, starch: 4), and the particle size and starch damage content were measured. To develop the classification model, four multivariate analysis methods (linear discriminant analysis (LDA), partial least-square discriminant analysis, support vector machine, and classification and regression tree) were implemented with several pre-processing methods, and their classification results were compared with respect to accuracy and Cohen’s kappa coefficient obtained from confusion matrices. (3) Results: The highest accuracy was achieved as 97.43% through short-wavelength infrared with normalization in the spectral domain. The submission of the developed classification model to the hyperspectral images showed that the fluorescence method achieves the highest accuracy of 81% using LDA. (4) Conclusions: In this study, the potential of non-destructive classification of rice and starch flours using multiple hyperspectral modalities and chemometric methods were demonstrated.

Non-destructive classification of defective potatoes based on hyperspectral imaging and support vector machine

2019 ◽  
Vol 99 ◽  
pp. 71-79 ◽  
Author(s):  
Yamin Ji ◽  
Laijun Sun ◽  
Yingsong Li ◽  
Jie Li ◽  
Shuangcai Liu ◽  
...  
Keyword(s):  
Support Vector Machine ◽  
Hyperspectral Imaging ◽  
Support Vector ◽  
Non Destructive

scholarly journals A Spectral Spatial Attention Fusion with Deformable Convolutional Residual Network for Hyperspectral Image Classification

2021 ◽  
Vol 13 (18) ◽  
pp. 3590
Author(s):  
Tianyu Zhang ◽  
Cuiping Shi ◽  
Diling Liao ◽  
Liguo Wang
Keyword(s):  
Image Classification ◽  
Spatial Attention ◽  
Hyperspectral Image ◽  
Spectral Feature ◽  
Hyperspectral Images ◽  
Spectral Features ◽  
Hyperspectral Image Classification ◽  
Residual Network ◽  
Spatial Features ◽  
Training Samples

Convolutional neural networks (CNNs) have exhibited excellent performance in hyperspectral image classification. However, due to the lack of labeled hyperspectral data, it is difficult to achieve high classification accuracy of hyperspectral images with fewer training samples. In addition, although some deep learning techniques have been used in hyperspectral image classification, due to the abundant information of hyperspectral images, the problem of insufficient spatial spectral feature extraction still exists. To address the aforementioned issues, a spectral–spatial attention fusion with a deformable convolution residual network (SSAF-DCR) is proposed for hyperspectral image classification. The proposed network is composed of three parts, and each part is connected sequentially to extract features. In the first part, a dense spectral block is utilized to reuse spectral features as much as possible, and a spectral attention block that can refine and optimize the spectral features follows. In the second part, spatial features are extracted and selected by a dense spatial block and attention block, respectively. Then, the results of the first two parts are fused and sent to the third part, and deep spatial features are extracted by the DCR block. The above three parts realize the effective extraction of spectral–spatial features, and the experimental results for four commonly used hyperspectral datasets demonstrate that the proposed SSAF-DCR method is superior to some state-of-the-art methods with very few training samples.

scholarly journals Non-Destructive Detection Pilot Study of Vegetable Organic Residues Using VNIR Hyperspectral Imaging and Deep Learning Techniques

Sensors ◽  
2021 ◽  
Vol 21 (9) ◽  
pp. 2899
Author(s):  
Youngwook Seo ◽  
Giyoung Kim ◽  
Jongguk Lim ◽  
Ahyeong Lee ◽  
Balgeum Kim ◽  
...  
Keyword(s):  
Deep Learning ◽  
Hyperspectral Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Imaging Technique ◽  
Support Vector ◽  
Organic Residues ◽  
Efficient Detection ◽  
Non Destructive

Contamination is a critical issue that affects food consumption adversely. Therefore, efficient detection and classification of food contaminants are essential to ensure food safety. This study applied a visible and near-infrared (VNIR) hyperspectral imaging technique to detect and classify organic residues on the metallic surfaces of food processing machinery. The experimental analysis was performed by diluting both potato and spinach juices to six different concentration levels using distilled water. The 3D hypercube data were acquired in the range of 400–1000 nm using a line-scan VNIR hyperspectral imaging system. Each diluted residue in the spectral domain was detected and classified using six classification methods, including a 1D convolutional neural network (CNN-1D) and five pre-processing methods. Among them, CNN-1D exhibited the highest classification accuracy, with a 0.99 and 0.98 calibration result and a 0.94 validation result for both spinach and potato residues. Therefore, in comparison with the validation accuracy of the support vector machine classifier (0.9 and 0.92 for spinach and potato, respectively), the CNN-1D technique demonstrated improved performance. Hence, the VNIR hyperspectral imaging technique with deep learning can potentially afford rapid and non-destructive detection and classification of organic residues in food facilities.

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