Primary tissues dominated ground-level trunk diameter in Sigillaria: evidence from the Wuda Tuff, Inner Mongolia

2021 ◽  
pp. jgs2021-021
Author(s):  
Michael P. D'Antonio ◽  
C. Kevin Boyce ◽  
Wei-Ming Zhou ◽  
Hermann W. Pfefferkorn ◽  
Jun Wang
Keyword(s):  
Inner Mongolia ◽  
Ground Level ◽  
Early Ontogeny ◽  
Internal Anatomy ◽  
Living Plant ◽  
Content Type ◽  
Trunk Diameter ◽  
Primary Body ◽  
Supplementary Material

Arborescent lycopsid stumps are typically fossilized as casts, so that proximal anatomy is rarely available for study. This presents a roadblock for empirically reconstructing their early ontogeny, which requires knowledge of proximal tissue proportions. Here, we describe nine upright, in situ stump casts of Sigillaria from the earliest Permian Wuda Tuff, Inner Mongolia, China that preserve traces of internal anatomy. From these specimens, we interpret thin vasculature and periderm and a broad primary cortex within ∼50 cm of ground level in the living plant. These specimens support recent arguments that periderm production was limited in arborescent lycopsids and that the primary body reached its mature diameter close to ground level rather than distally further up the trunk.Supplementary material: additional figures of specimens and data regarding where measurements were made is available at https://doi.org/10.6084/m9.figshare.c.5558620

Geochemical evaluation of the in-situ regolith of Madengi hills of Dodoma, Tanzania. Implication on bedrock mapping and delineating gold mineralization target

2021 ◽  
pp. geochem2021-074
Author(s):  
Godson Godfray
Keyword(s):  
Gold Mineralization ◽  
Early Stage ◽  
Shear Zones ◽  
Soil Geochemistry ◽  
Residual Soils ◽  
Content Type ◽  
Geochemical Evaluation ◽  
Supplementary Material ◽  
Pathfinder Elements

Successful gold exploration projects depend on a piece of clear information on the association between gold, trace elements, and mineralization controlling factors. The use of soil geochemistry has been an important tool in pinpointing exploration targets during the early stage of exploration. This study aimed to establish the gold distribution, the elemental association between gold and its pathfinder elements such as Cu, Zn, Ag, Ni, Co, Mn, Fe, Cd, V, Cr, Ti, Sc, In, and Se and identify lithologies contributing to the overlying residual soils. From cluster analysis, a high similarity level of 53.93% has been shown with Ag, Cd, and Se at a distance level of 0.92. Au and Se have a similarity level of 65.87% and a distance level of 0.68, hence is proposed to be the most promising pathfinder element. PCA, FA, and the Pearson's correlation matrix of transformed data of V, Cu, Ni, Fe, Mn, Cr, and Co and a stronger correlation between Pb and U, Th, Na, K, Sn, Y, Ta and Be shows that source gold mineralization might be associated with both hornblende gneisses interlayered with quartzite, tonalite, and tonalitic orthogneiss. From the contour map and gridded map of Au and its pathfinder elements, it has been noted that their anomalies and target generated are localized in the Northern part of the area. The targets trend ESE to WNW nearly parallel to the shear zones as a controlling factor of Au mineralization emplacement.Supplementary material:https://doi.org/10.6084/m9.figshare.c.5721965

In-situ laser ablation Lu–Hf geochronology of garnet across the Western Gneiss Region: Campaign-style dating of metamorphism

2021 ◽  
pp. jgs2021-094
Author(s):  
Renée Tamblyn ◽  
Martin Hand ◽  
Alexander Simpson ◽  
Sarah Gilbert ◽  
Ben Wade ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Ultrahigh Pressure ◽  
Western Gneiss Region ◽  
Content Type ◽  
Link Type ◽  
Geological Processes ◽  
History Of ◽  
Supplementary Material ◽  
Wilson Cycle

The development of in-situ laser ablation Lu–Hf geochronology of apatite, xenotime and garnet has opened avenues to quickly and directly date geological processes. We demonstrate the first use of campaign-style in-situ Lu–Hf geochronology of garnet across the high- to ultrahigh-pressure Western Gneiss Region in Norway. Mafic eclogites from this region have been the focus of much work, and were clearly formed during continental subduction during the Caledonian Orogen. However, abundant quartzofeldspathic and pelitic lithologies record a more complex history, with some preserving polymetamorphic age data, and most containing no indication of high-pressure mineral assemblages formed during subduction. Twenty metapelitic and felsic samples spanning 160 lateral kilometers across the Western Gneiss Region have been analysed using garnet Lu–Hf geochronology. The results reveal Caledonian ages for the majority of the garnets, suggesting some quartzofeldspathic and metapelitic lithologies were reactive and grew garnet during high- to ultrahigh-pressure metamorphism. However, two ultrahigh-pressure eclogite locations, Verpeneset and Fjørtoft, preserve both Caledonian and Neoproterozoic-aged garnets. Despite significant uncertainties on some of the Lu–Hf geochronologic ages, laser ablation Lu–Hf efficiently identifies the polymetamorphic history of parts of the Western Gneiss Region, illustrating the effectiveness of this novel analytical method for rapid mapping of metamorphic ages.Thematic collection: This article is part of the Caledonian Wilson cycle collection available at: https://www.lyellcollection.org/cc/caledonian-wilson-cycleSupplementary material:https://doi.org/10.6084/m9.figshare.c.5715453

Did an earthquake located off Jersey trigger a mudflow preserving the only loess outcrop known under the seas?

2020 ◽  
Vol 177 (5) ◽  
pp. 911-922
Author(s):  
Jean-Pierre Lefort ◽  
Paul Chambers ◽  
Guzel Danukalova ◽  
Jean-Laurent Monnier ◽  
Eugenia Osipova ◽  
...  
Keyword(s):  
Slope Failure ◽  
Core Sample ◽  
Seismic Zone ◽  
Content Type ◽  
The Core ◽  
Loess Deposit ◽  
Mollusc Species ◽  
Supplementary Material ◽  
Rule Out

A 5 m long core sample, retrieved from between Jersey and the Cotentin (France) has been studied using different techniques. Between the lower part and the top, the core is a yellowish loess, a black mud and a grey shelly formation. The basal formation, which displays a typical loess granulometry, is the only loess found anywhere in situ on the subtidal seabed worldwide. Elsewhere, this fragile sediment has always been washed out by the successive Quaternary transgressions. The overlying mud formation, which displays characteristics of a landslide, sealed the loess deposit. Because this core has been sampled on the rim of a submarine valley located in front of a major onshore geological disruption, clearly associated with an active seismic zone, it is possible that an earthquake triggered a slope failure causing a gravity collapse of materials that protected the loess deposit from subsequent erosion, although we cannot completely rule out the effects of a large flood.Supplementary material: Tables showing the results of the pollen analysis and distribution of mollusc shells and other fossils in borehole O-VC15, and a figure showing the distribution of mollusc species in core O-VC15 and a possible reconstruction of the palaeoenvironment during sediment deposition are available at https://doi.org/10.6084/m9.figshare.c.4991753

Anatomy of two Permian greenschist- to blueschist-facies tectonic mélanges in the Solonker Suture Zone (Inner Mongolia, northeastern China): evidence for divergent double subduction and soft collision

2020 ◽  
Vol 177 (5) ◽  
pp. 981-996 ◽  
Author(s):  
Huichuan Liu ◽  
Jichun Wang ◽  
Chun-Kit Lai ◽  
Yinglei Li ◽  
Jingfan Wang ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Northeastern China ◽  
Controversial Issues ◽  
Early Permian ◽  
Content Type ◽  
The Matrix ◽  
Ophiolitic Rocks ◽  
Supplementary Material ◽  
Tectonic Mélange ◽  
East West

The timing and processes of the Paleo-Asian Ocean (PAO) closure are still controversial issues due to ambiguous interpretation of the regional geological records. We describe the structural and kinematic features of two Permian greenschist-/blueschist-facies tectonic mélanges at Zhurihe and Erdaojing in Inner Mongolia (northeastern China), separated by the Solonker Suture. The tectonic mélanges have block-in-matrix fabrics. The Zhurihe tectonic mélange in the south is dominated by NE-trending folds, and east–west- to NE-trending reverse/strike-slip faults, indicating a NW–SE compressive direction. Blocks in the Zhurihe mélange include ophiolitic fragments (c. 292 Ma) and ocean island sequences (c. 274 Ma), which show E-MORB and OIB geochemical affinities, respectively. The matrix includes blueschist, greenschist, quartz schist and clastics, of which the blueschist yielded a zircon U–Pb age of c. 255 Ma, and is geochemically E-MORB-like. The Erdaojing mélange north of the Solonker Suture is dominated by east–west-trending folds, and NE- and NW-trending reverse faults, indicating a north–south compressive orientation. The Erdaojing mélange is composed of early Permian (c. 281 Ma) ophiolite blocks, and middle Permian (c. 266 Ma) actinolite schist and clastics as the matrix. The Erdaojing ophiolitic rocks are geochemically N-MORB tholeiitic. Our results define two parallel Permian MORB-type ophiolitic belts separated by the central Solonker Suture. These observations are evidence for the Permian divergent double subduction and Early Triassic soft-collision model for the PAO along the Solonker Suture.Supplementary material: Detailed analytical methods and results are available at: https://doi.org/10.6084/m9.figshare.c.5008910

Pituitary adenoma producing growth hormone and adrenocorticotropin: a histological, immunocytochemical, electron microscopic, and in situ hybridization study

1998 ◽  
Vol 88 (6) ◽  
pp. 1111-1115 ◽  
Author(s):  
Kalman Kovacs ◽  
Eva Horvath ◽  
Lucia Stefaneanu ◽  
Juan Bilbao ◽  
William Singer ◽  
...  
Keyword(s):  
Growth Hormone ◽  
In Situ Hybridization ◽  
Pituitary Adenoma ◽  
Immunoelectron Microscopy ◽  
Secretory Granules ◽  
Cell Types ◽  
Content Type ◽  
Separate Cell ◽  
Fine Print

✓ The authors report on the morphological features of a pituitary adenoma that produced growth hormone (GH) and adrenocorticotropic hormone (ACTH). This hormone combination produced by a single adenoma is extremely rare; a review of the available literature showed that only one previous case has been published. The tumor, which was removed from a 62-year-old man with acromegaly, was studied by histological and immunocytochemical analyses, transmission electron microscopy, immunoelectron microscopy, and in situ hybridization. When the authors used light microscopy, the tumor appeared to be a bimorphous mixed pituitary adenoma composed of two separate cell types: one cell population synthesized GH and the other ACTH. The cytogenesis of pituitary adenomas that produce more than one hormone is obscure. It may be that two separate cells—one somatotroph and one corticotroph—transformed into neoplastic cells, or that the adenoma arose in a common stem cell that differentiated into two separate cell types. In this case immunoelectron microscopy conclusively demonstrated ACTH in the secretory granules of several somatotrophs. This was associated with a change in the morphological characteristics of secretory granules. Thus it is possible that the tumor was originally a somatotropic adenoma that began to produce ACTH as a result of mutations that occurred during tumor progression.

scholarly journals Competition between Metals for Binding to Methanobactin Enables Expression of Soluble Methane Monooxygenase in the Presence of Copper

2014 ◽  
Vol 81 (3) ◽  
pp. 1024-1031 ◽  
Author(s):  
Bhagyalakshmi Kalidass ◽  
Muhammad Farhan Ul-Haque ◽  
Bipin S. Baral ◽  
Alan A. DiSpirito ◽  
Jeremy D. Semrau
Keyword(s):  
Methane Monooxygenase ◽  
High Specificity ◽  
Copper Uptake ◽  
Content Type ◽  
Soluble Methane Monooxygenase ◽  
Sds Page ◽  
Genes Encoding ◽  
Key Factor ◽  
Membrane Bound

ABSTRACTIt is well known that copper is a key factor regulating expression of the two forms of methane monooxygenase found in proteobacterial methanotrophs. Of these forms, the cytoplasmic, or soluble, methane monooxygenase (sMMO) is expressed only at low copper concentrations. The membrane-bound, or particulate, methane monooxygenase (pMMO) is constitutively expressed with respect to copper, and such expression increases with increasing copper. Recent findings have shown that copper uptake is mediated by a modified polypeptide, or chalkophore, termed methanobactin. Although methanobactin has high specificity for copper, it can bind other metals, e.g., gold. Here we show that inMethylosinus trichosporiumOB3b, sMMO is expressed and active in the presence of copper if gold is also simultaneously present. Such expression appears to be due to gold binding to methanobactin produced byM. trichosporiumOB3b, thereby limiting copper uptake. Such expression and activity, however, was significantly reduced if methanobactin preloaded with copper was also added. Further, quantitative reverse transcriptase PCR (RT-qPCR) of transcripts of genes encoding polypeptides of both forms of MMO and SDS-PAGE results indicate that both sMMO and pMMO can be expressed when copper and gold are present, as gold effectively competes with copper for binding to methanobactin. Such findings suggest that under certain geochemical conditions, both forms of MMO may be expressed and activein situ. Finally, these findings also suggest strategies whereby field sites can be manipulated to enhance sMMO expression, i.e., through the addition of a metal that can compete with copper for binding to methanobactin.

scholarly journals Specificity of Associations between Bacteria and the Coral Pocillopora meandrina during Early Development

2012 ◽  
Vol 78 (20) ◽  
pp. 7467-7475 ◽  
Author(s):  
Amy Apprill ◽  
Heather Q. Marlow ◽  
Mark Q. Martindale ◽  
Michael S. Rappé
Keyword(s):  
Small Subunit ◽  
Rrna Genes ◽  
Polymorphism Analysis ◽  
Roseobacter Clade ◽  
Small Subunit Rrna ◽  
Content Type ◽  
Sterile Seawater ◽  
Show Evidence ◽  
Coral Health

ABSTRACTRelationships between corals and specific bacterial associates are thought to play an important role in coral health. In this study, the specificity of bacteria associating with the coralPocillopora meandrinawas investigated by exposing coral embryos to various strains of cultured marine bacteria, sterile seawater, or raw seawater and examining the identity, density, and location of incorporated cells. The isolates utilized in this experiment included members of the Roseobacter and SAR11 clades of theAlphaproteobacteria, aPseudoalteromonasspecies of theGammaproteobacteria, and aSynechococcusspecies of theCyanobacteriaphylum. Based on terminal restriction fragment length polymorphism analysis of small-subunit rRNA genes, similarities in bacterial communities associated with 170-h-old planulae were observed regardless of treatment, suggesting that bacteria may have been externally associated from the outset of the experiment. Microscopic examination ofP. meandrinaplanulae by fluorescencein situhybridization with bacterial and Roseobacter clade-specific oligonucleotide probes revealed differences in the densities and locations of planulae-associated cells. Planulae exposed to either raw seawater or strains ofPseudoalteromonasand Roseobacter harbored the highest densities of internally associated cells, of which 20 to 100% belonged to the Roseobacter clade. Planulae exposed to sterile seawater or strains of the SAR11 clade andSynechococcusdid not show evidence of prominent bacterial associations. Additional analysis of the raw-seawater-exposed planulae via electron microscopy confirmed the presence of internally associated prokaryotic cells, as well as virus-like particles. These results suggest that the availability of specific microorganisms may be an important factor in the establishment of coral-bacterial relationships.

Methodology for in situ stress intensity factor determination on cracked structures by digital image correlation

2010 ◽  
Vol 1 (4) ◽  
pp. 344-357 ◽  
Author(s):  
V. Richter‐Trummer ◽  
P.M.G.P. Moreira ◽  
S.D. Pastrama ◽  
M.A.P. Vaz ◽  
P.M.S.T. de Castro
Keyword(s):  
Stress Intensity Factor ◽  
Stress Intensity ◽  
Digital Image Correlation ◽  
Intensity Factor ◽  
Digital Image ◽  
Strain Field ◽  
Image Correlation ◽  
Content Type ◽  
Cracked Structures

PurposeThe purpose of this paper is to develop a methodology for in situ stress intensity factor (SIF) determination that can be used for the analysis of cracked structures. The technique is based on digital image correlation (DIC) combined with an overdetermined algorithm.Design/methodology/approachThe linear overdeterministic algorithm for calculating the SIF based on stress values around the crack tip is applied to a strain field obtained by DIC.FindingsAs long as the image quality is sufficiently high, a good accuracy can be obtained for the measured SIF. The crack tip can be automatically detected based on the same strain field. The use of the strain field instead of the displacement field, eliminates problems related to the rigid body motion of the analysed structure.Practical implicationsIn future works, based on the applied techniques, the SIF of complex cracked plane stress structures can be accurately determined in real engineering applications.Originality/valueThe paper demonstrates application of known techniques, refined for other applications, also the use of stress field for SIF overdeterministic calculations.

scholarly journals Pyrobaculum yellowstonensis Strain WP30 Respires on Elemental Sulfur and/or Arsenate in Circumneutral Sulfidic Geothermal Sediments of Yellowstone National Park

2015 ◽  
Vol 81 (17) ◽  
pp. 5907-5916 ◽  
Author(s):  
Z. J. Jay ◽  
J. P. Beam ◽  
A. Dohnalkova ◽  
R. Lohmayer ◽  
B. Bodle ◽  
...  
Keyword(s):  
Yellowstone National Park ◽  
National Park ◽  
Elemental Sulfur ◽  
De Novo ◽  
Hot Spring ◽  
Content Type ◽  
Physiological Attributes ◽  
And Function ◽  
Metagenome Sequence

ABSTRACTThermoproteales(phylumCrenarchaeota) populations are abundant in high-temperature (>70°C) environments of Yellowstone National Park (YNP) and are important in mediating the biogeochemical cycles of sulfur, arsenic, and carbon. The objectives of this study were to determine the specific physiological attributes of the isolatePyrobaculum yellowstonensisstrain WP30, which was obtained from an elemental sulfur sediment (Joseph's Coat Hot Spring [JCHS], 80°C, pH 6.1, 135 μM As) and relate this organism to geochemical processes occurringin situ. Strain WP30 is a chemoorganoheterotroph and requires elemental sulfur and/or arsenate as an electron acceptor. Growth in the presence of elemental sulfur and arsenate resulted in the formation of thioarsenates and polysulfides. The complete genome of this organism was sequenced (1.99 Mb, 58% G+C content), revealing numerous metabolic pathways for the degradation of carbohydrates, amino acids, and lipids. Multiple dimethyl sulfoxide-molybdopterin (DMSO-MPT) oxidoreductase genes, which are implicated in the reduction of sulfur and arsenic, were identified. Pathways for thede novosynthesis of nearly all required cofactors and metabolites were identified. The comparative genomics ofP. yellowstonensisand the assembled metagenome sequence from JCHS showed that this organism is highly related (∼95% average nucleotide sequence identity) toin situpopulations. The physiological attributes and metabolic capabilities ofP. yellowstonensisprovide an important foundation for developing an understanding of the distribution and function of these populations in YNP.

scholarly journals Enzymatic Degradation of Phenazines Can Generate Energy and Protect Sensitive Organisms from Toxicity

mBio ◽  
2015 ◽  
Vol 6 (6) ◽  
Author(s):  
Kyle C. Costa ◽  
Megan Bergkessel ◽  
Scott Saunders ◽  
Jonas Korlach ◽  
Dianne K. Newman
Keyword(s):  
Microbial Communities ◽  
Pathogenic Fungi ◽  
Cell Types ◽  
Redox Activity ◽  
Mycobacterium Fortuitum ◽  
Active Metabolites ◽  
Content Type ◽  
Phenazine Production ◽  
Physiological Benefits

ABSTRACTDiverse bacteria, including severalPseudomonasspecies, produce a class of redox-active metabolites called phenazines that impact different cell types in nature and disease. Phenazines can affect microbial communities in both positive and negative ways, where their presence is correlated with decreased species richness and diversity. However, little is known about how the concentration of phenazines is modulatedin situand what this may mean for the fitness of members of the community. Through culturing of phenazine-degrading mycobacteria, genome sequencing, comparative genomics, and molecular analysis, we identified several conserved genes that are important for the degradation of threePseudomonas-derived phenazines: phenazine-1-carboxylic acid (PCA), phenazine-1-carboxamide (PCN), and pyocyanin (PYO). PCA can be used as the sole carbon source for growth by these organisms. Deletion of several genes inMycobacterium fortuitumabolishes the degradation phenotype, and expression of two genes in a heterologous host confers the ability to degrade PCN and PYO. In cocultures with phenazine producers, phenazine degraders alter the abundance of different phenazine types. Not only does degradation support mycobacterial catabolism, but also it provides protection to bacteria that would otherwise be inhibited by the toxicity of PYO. Collectively, these results serve as a reminder that microbial metabolites can be actively modified and degraded and that these turnover processes must be considered when the fate and impact of such compounds in any environment are being assessed.IMPORTANCEPhenazine production byPseudomonasspp. can shape microbial communities in a variety of environments ranging from the cystic fibrosis lung to the rhizosphere of dryland crops. For example, in the rhizosphere, phenazines can protect plants from infection by pathogenic fungi. The redox activity of phenazines underpins their antibiotic activity, as well as providing pseudomonads with important physiological benefits. Our discovery that soil mycobacteria can catabolize phenazines and thereby protect other organisms against phenazine toxicity suggests that phenazine degradation may influence turnoverin situ. The identification of genes involved in the degradation of phenazines opens the door to monitoring turnover in diverse environments, an essential process to consider when one is attempting to understand or control communities influenced by phenazines.

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