Predictors of adolescents’ response to Young Person’s Face IT: A web-based intervention to improve psychosocial adjustment to having an appearance-affecting condition (Preprint)

BACKGROUND Adolescents with a condition affecting their appearance that results in a visible difference can be at risk of psychosocial distress and impaired adjustment. Evidence for the effectiveness of existing interventions in improving psychosocial outcomes is limited and relevant treatment can be difficult to access. Young Person’s Face IT (YPF), a novel self-guided web-based intervention, has demonstrated potential in reducing social anxiety in adolescents with visible differences. However, more knowledge is needed regarding factors that contribute to variations in intervention effects in order to identify which adolescents may benefit most from YPF. OBJECTIVE This study aimed to identify predictors related to overall intervention effects following adolescents’ use of YPF. METHODS N=71 adolescents (61% girls; mean age 13.98, range 11–18 years) with a wide range of visible differences completed primary (body esteem and social anxiety symptoms) and secondary (perceived stigmatization, life disengagement, and self-rated health) outcome measures, at baseline and post-intervention. Predictor variables were demographic (age and gender), psychosocial (frequency of teasing experiences related to aspects of body and appearance, and depressive and/or anxiety symptoms), and intervention-related (time spent on YPF) factors. RESULTS Backward multiple regression revealed that higher intervention effects were predicted by gender, baseline frequency of teasing experiences, levels of depressive and/or anxiety symptoms, and time spent on YPF. However, the results were limited by low proportion of explained post-intervention variance, ranging from 1.6 to 24.1%. CONCLUSIONS This study suggests that adolescent boys, adolescents who experience higher levels of psychosocial distress related to their visible difference, and adolescents that spend sufficient time on YPF, may obtain better overall intervention effects. CLINICALTRIAL NCT03165331

Maladaptive Self-Focused Attention and Default Mode Network Connectivity: A Transdiagnostic Investigation Across Social Anxiety and Body Dysmorphic Disorders

Maladaptive self-focused attention (SFA) is a bias toward internal thoughts, feelings, and physical states. Despite its role as a core maintaining factor of symptoms in cognitive theories of social anxiety and body dysmorphic disorders, studies have not examined its neural basis. In this study, we hypothesized that maladaptive SFA would be associated with hyperconnectivity in the default mode network (DMN) in self-focused patients with these disorders. Thirty patients with primary social anxiety disorder or primary body dysmorphic disorder, and 28 healthy individuals were eligible and scanned. Eligibility was determined by scoring greater than 1SD or below 1SD of the Public Self-Consciousness Scale normative mean, respectively, for each group. Seed-to-voxel functional connectivity was computed using a DMN posterior cingulate cortex (PCC) seed. There was no evidence of increased DMN functional connectivity in patients compared to controls. Patients (regardless of diagnosis) showed reduced functional connectivity of the PCC with several brain regions, including the bilateral superior parietal lobule (SPL), compared to controls, which was inversely correlated with maladaptive SFA but not associated with social anxiety, body dysmorphic, or depression severity, or rumination. Abnormal PCC-SPL connectivity may represent a transdiagnostic neural marker of SFA that reflects difficulty shifting between internal versus external attention.

Reduced serum albumin as a risk factor for poor prognosis in critically ill patients receiving renal replacement therapy

Background Albumin is the primary body protein, which can predict the poor prognosis of several critical diseases. However, there are a few scientific studies on the relationship between albumin and the prognosis of dialysis patients. This study aims to explore the impact of hypoalbuminemia on the prognosis of critically ill patients with acute kidney injury (AKI) receiving continuous renal replacement therapy (CRRT). Methods This was a secondary study. Clinical, biochemical, and 28-day and 90-day mortality rates for critical patients with AKI who received CRRT between 2009 and 2016 were searched from the database to determine the effect of hypoalbuminemia on poor outcomes by univariate, multivariate, smooth curve fitting, and subgroup analysis. Results A total of 837 participants were enrolled in this study. Multivariate Cox proportional hazard regression analysis showed that hypoalbuminemia was associated with both 28-day and 90-day mortality risks after full adjustment for confounding variables, with an adjusted hazard ratio (95% confidence interval) of 0.63 (0.50–0.80) and 0.63 (0.51–0.78), respectively for each 1 g/dL increase of albumin. Stratified analysis showed that hypoalbuminemia was not associated with poor prognosis in oliguria. Conclusion Hypoalbuminemia is associated with poor prognosis in critically ill AKI patients with CRRT; therefore, measuring albumin may be helpful for predicting the prognosis. However, in those with oliguria, this conclusion is not valid.

The pericardium of Oikopleura dioica (Tunicata, Appendicularia) contains two distinct cell types and is rotated by 90 degrees to the left

The planktonic Oikopleura dioica belongs to Tunicata, the probable sister taxon to Craniota, and might show plesiomorphic characters, conserved from the common lineage of Tunicata and Craniota. In O. dioica a pericardium in a position similar to other chordates but also to the heart and pericardium of craniates is found. Surprisingly, little is known about the ultrastructure of the pericardium in O. dioica. Here, we show based on electron microscopy that the pericardium is completely lined by a single layer of 16 epithelial cells: 6 epithelial myocardial cells on the left side of the pericardium and 10 peritoneal cells constituting the right side. One of the peritoneal cells, situated at the ventral border between peritoneal cells and myocardial cells has an extension that anchors the pericardium to the basal lamina beneath the latero-ventral epidermis. The primary body cavity of O. dioica appears quite uniformly clear in electron microscopic aspect but several sheets, resembling the basal lamina of the pericardium cross the larger spaces of the body cavity and connect to the pericardial basal lamina. This is the first detailed description of two distinct cell types in the epithelial lining of the pericardium of O. dioica. In comparison with other chordates, we conclude that two cell types can be reconstructed for the last common ancestor of Chordata at least. The position of the pericardium at the intersection of trunk and tail in combination with the basal-lamina like sheets spanning the hemocoel is probably of importance for the function of the circulation of the hemocoelic fluid. Similar to the tail, the axis of the pericardium is shifted through 90 degrees to the left as compared to the main body axis of the trunk and we infer that this shift is an apomorphic character of Appendicularia.

Studying evolution of the primary body axis in vivo and in vitro

The metazoan body plan is established during early embryogenesis via collective cell rearrangements and evolutionarily conserved gene networks, as part of a process commonly referred to as gastrulation. While substantial progress has been achieved in terms of characterizing the embryonic development of several model organisms, underlying principles of many early patterning processes nevertheless remain enigmatic. Despite the diversity of (pre-)gastrulating embryo and adult body shapes across the animal kingdom, the body axes, which are arguably the most fundamental features, generally remain identical between phyla. Recently there has been a renewed appreciation of ex vivo and in vitro embryo-like systems to model early embryonic patterning events. Here, we briefly review key examples and propose that similarities in morphogenesis and associated gene expression dynamics may reveal an evolutionarily conserved developmental mode as well as provide further insights into the role of external or extraembryonic cues in shaping the early embryo. In summary, we argue that embryo-like systems can be employed to inform previously uncharted aspects of animal body plan evolution as well as associated patterning rules.

Beyond body maps: information content of specific body parts is distributed across the somatosensory Homunculus

The somatosensory homunculus in primary somatosensory cortex (S1) is topographically organised, with relatively high selectivity to each body part in its primary area. This dominant feature may eclipse other organising principles in S1. Recent multivariate methodologies allow us to identify representational features beyond selectivity, e.g., information content, providing new opportunities to characterise the homunculus. Using Representational Similarity Analysis, we asked whether body part information content can be identified in S1 beyond the primary area of a given body part. Representational dissimilarities in fMRI activity patterns were compared between different body parts (face, hand and feet) and subparts (e.g., fingers), and between different actions performed with the same body part. Throughout the S1 homunculus, we identified significant dissimilarities between non-primary body parts (e.g., between the hand and the lips in the foot area). We also observed significant dissimilarities between body subparts in distant non-primary areas (e.g., different face parts in the foot area). Finally, we could significantly dissociate between two movements performed by one body part (e.g., the hand) well beyond its primary area (e.g., in the foot and face areas), even when focusing the analysis along the most topographically organised sub-region of S1, Brodmann area 3b. Altogether, our results demonstrate that body part and action-related information content is more distributed across S1 homunculus than previously considered. While this finding does not revoke the general topographic organising principle of S1, it reveals yet unexplored underlying information contents that could be harnessed for rehabilitation, as well as novel brain-machine interfaces.

Primary tissues dominated ground-level trunk diameter in Sigillaria: evidence from the Wuda Tuff, Inner Mongolia

Arborescent lycopsid stumps are typically fossilized as casts, so that proximal anatomy is rarely available for study. This presents a roadblock for empirically reconstructing their early ontogeny, which requires knowledge of proximal tissue proportions. Here, we describe nine upright, in situ stump casts of Sigillaria from the earliest Permian Wuda Tuff, Inner Mongolia, China that preserve traces of internal anatomy. From these specimens, we interpret thin vasculature and periderm and a broad primary cortex within ∼50 cm of ground level in the living plant. These specimens support recent arguments that periderm production was limited in arborescent lycopsids and that the primary body reached its mature diameter close to ground level rather than distally further up the trunk.Supplementary material: additional figures of specimens and data regarding where measurements were made is available at https://doi.org/10.6084/m9.figshare.c.5558620

DEVELOPMENTAL AND EVOLUTIONARY COMPARATIVE ANALYSIS OF A HOXD REGULATORY LANDSCAPE IN MAMMALS AND BIRDS

Modifications in gene regulation during development are considered to be a driving force in the evolution of organisms. Part of these changes involve rapidly evolving cis-regulatory elements (CREs), which interact with their target genes through higher-order 3D chromatin structures. How such 3D architectures and variations in CREs contribute to transcriptional evolvability nevertheless remains elusive. During vertebrate evolution, Hox genes were redeployed in different organs in a class-specific manner, while maintaining the same basic function in organizing the primary body axis. Since a large part of the relevant enhancers are located in a conserved regulatory landscape, this gene cluster represents an interesting paradigm to study the emergence of regulatory innovations. In this work, we analyzed Hoxd gene regulation in both murine vibrissae and chicken feather primordia, two mammalian- and avian-specific skin appendages which express different subsets of Hoxd genes, and compared their regulatory modalities with the regulations at work during the elongation of the posterior trunk, a mechanism highly conserved in amniotes. We show that in the former two structures, distinct subsets of Hoxd genes are contacted by different lineage-specific enhancers, likely as a result of using an ancestral chromatin topology as an evolutionary playground, whereas the regulations implemented in the mouse and chicken embryonic trunk rely more on conserved CREs. Nevertheless, a high proportion of these non-coding sequences active in the trunk appear to have functionally diverged between the two species, suggesting that transcriptional robustness is maintained despite a considerable divergence in CRE sequences, an observation supported by a genome-wide comparative approach.

The Map and the Territory

“I didn’t even know that was a question I could ask.” That remark from a student in an introductory philosophy course points to the primary body of knowledge philosophy produces: a detailed record of what we do not know. When we come to view a philosophical question as well-formed and worthwhile, it is a way of providing as specific a description as we can of something we do not know. The creation or discovery of such questions is like noting a landmark in a territory we’re exploring. When we identify reasonable, if conflicting, answers to this question, we are noting routes to and away from that landmark. And since proposed answers to philosophical questions often contain implied answers to other philosophical questions, those routes connect different landmarks. The result is a kind of map: a map of the unknown. Yet when it comes to the unknown, and all the more so to its cartography, might it not make sense to take our orientation from Borges: What’s in question here, with respect to philosophical questions, is an incipient, unlocalizable threshold—a terrain neither subjective, nor entirely objective, one neither of representation, nor finally of simple immediacy—there where the map perceptibly fails to diverge from the territory. Amid Inclemencies of weather and fringed, as per Borges, with ruin and singular figures—with Animals and Beggars—what’s enclosed is an attempt to chart the contours of this curious immanence.

The Wnt-specific astacin proteinase HAS-7 restricts head organizer formation in Hydra

Background The Hydra head organizer acts as a signaling center that initiates and maintains the primary body axis in steady state polyps and during budding or regeneration. Wnt/beta-Catenin signaling functions as a primary cue controlling this process, but how Wnt ligand activity is locally restricted at the protein level is poorly understood. Here we report a proteomic analysis of Hydra head tissue leading to the identification of an astacin family proteinase as a Wnt processing factor. Results Hydra astacin-7 (HAS-7) is expressed from gland cells as an apical-distal gradient in the body column, peaking close beneath the tentacle zone. HAS-7 siRNA knockdown abrogates HyWnt3 proteolysis in the head tissue and induces a robust double axis phenotype, which is rescued by simultaneous HyWnt3 knockdown. Accordingly, double axes are also observed in conditions of increased Wnt activity as in transgenic actin::HyWnt3 and HyDkk1/2/4 siRNA treated animals. HyWnt3-induced double axes in Xenopus embryos could be rescued by coinjection of HAS-7 mRNA. Mathematical modelling combined with experimental promotor analysis indicate an indirect regulation of HAS-7 by beta-Catenin, expanding the classical Turing-type activator-inhibitor model. Conclusions We show the astacin family protease HAS-7 maintains a single head organizer through proteolysis of HyWnt3. Our data suggest a negative regulatory function of Wnt processing astacin proteinases in the global patterning of the oral-aboral axis in Hydra.