Membrane-Protein Interactions in Cell Signaling and Membrane Trafficking

2005 ◽  
Vol 34 (1) ◽  
pp. 119-151 ◽  
Author(s):  
Wonhwa Cho ◽  
Robert V. Stahelin
Keyword(s):  
Membrane Protein ◽  
Cell Signaling ◽  
Protein Interactions ◽  
Membrane Trafficking

scholarly journals Mapping membrane protein interactions in cell signaling systems.

2003 ◽  
Author(s):  
Yooli Kim Light ◽  
Masood Z. Hadi ◽  
Pamela Lane ◽  
Richard B. Jacobsen ◽  
Joohee Hong ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Cell Signaling ◽  
Protein Interactions ◽  
Signaling Systems

scholarly journals Current Methods for Detecting Cell Membrane Transient Interactions

2020 ◽  
Vol 8 ◽  
Author(s):  
Yousef Bagheri ◽  
Ahsan Ausaf Ali ◽  
Mingxu You
Keyword(s):  
Cell Membrane ◽  
Membrane Protein ◽  
Cell Signaling ◽  
Membrane Trafficking ◽  
Experimental Methods ◽  
New Techniques ◽  
Lipid Interactions ◽  
Cellular Events ◽  
Transient Interactions ◽  
Cell Membrane Protein

Short-lived cell membrane complexes play a key role in regulating cell signaling and communication. Many of these complexes are formed based on low-affinity and transient interactions among various lipids and proteins. New techniques have emerged to study these previously overlooked membrane transient interactions. Exciting functions of these transient interactions have been discovered in cellular events such as immune signaling, host–pathogen interactions, and diseases such as cancer. In this review, we have summarized current experimental methods that allow us to detect and analyze short-lived cell membrane protein–protein, lipid–protein, and lipid–lipid interactions. These methods can provide useful information about the strengths, kinetics, and/or spatial patterns of membrane transient interactions. However, each method also has its own limitations. We hope this review can be used as a guideline to help the audience to choose proper approaches for studying membrane transient interactions in different membrane trafficking and cell signaling events.

scholarly journals Impact of Environmental Stressors on Gene Expression in the Embryo of the Italian Wall Lizard

2021 ◽  
Vol 11 (11) ◽  
pp. 4723
Author(s):  
Rosaria Scudiero ◽  
Chiara Maria Motta ◽  
Palma Simoniello
Keyword(s):  
Gene Expression ◽  
Protein Interactions ◽  
Membrane Trafficking ◽  
Translational Regulation ◽  
Cellular Protection ◽  
Terrestrial Vertebrate ◽  
Expression Of Genes ◽  
Stress Changes ◽  
Probable Consequence ◽  
The Impact

The cleidoic eggs of oviparous reptiles are protected from the external environment by membranes and a parchment shell permeable to water and dissolved molecules. As a consequence, not only physical but also chemical insults can reach the developing embryos, interfering with gene expression. This review provides information on the impact of the exposure to cadmium contamination or thermal stress on gene expression during the development of Italian wall lizards of the genus Podarcis. The results obtained by transcriptomic analysis, although not exhaustive, allowed to identify some stress-reactive genes and, consequently, the molecular pathways in which these genes are involved. Cadmium-responsive genes encode proteins involved in cellular protection, metabolism and proliferation, membrane trafficking, protein interactions, neuronal transmission and plasticity, immune response, and transcription regulatory factors. Cold stress changes the expression of genes involved in transcriptional/translational regulation and chromatin remodeling and inhibits the transcription of a histone methyltransferase with the probable consequence of modifying the epigenetic control of DNA. These findings provide transcriptome-level evidence of how terrestrial vertebrate embryos cope with stress, giving a key to use in population survival and environmental change studies. A better understanding of the genes contributing to stress tolerance in vertebrates would facilitate methodologies and applications aimed at improving resistance to unfavourable environments.

scholarly journals A microfluidic strategy for the detection of membrane protein interactions

Lab on a Chip ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 3230-3238
Author(s):  
Yuewen Zhang ◽  
Therese W. Herling ◽  
Stefan Kreida ◽  
Quentin A. E. Peter ◽  
Tadas Kartanas ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Interactions ◽  
Native Solution ◽  
Exchange Of Information ◽  
Extracellular Environment

Membrane proteins are gatekeepers for exchange of information and matter between the intracellular and extracellular environment. This paper opens up a route to probe membrane protein interactions under native solution conditions using microfluidics.

scholarly journals A Simple Detection Method for Low-Affinity Membrane Protein Interactions by Baculoviral Display

PLoS ONE ◽  
2008 ◽  
Vol 3 (12) ◽  
pp. e4024 ◽  
Author(s):  
Toshiko Sakihama ◽  
Takato Sato ◽  
Hiroko Iwanari ◽  
Toshio Kitamura ◽  
Shimon Sakaguchi ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Interactions ◽  
Detection Method ◽  
Affinity Membrane ◽  
Simple Detection

scholarly journals Sex Hormones Regulate Rat Hepatic Monocarboxylate Transporter Expression and Membrane Trafficking

2017 ◽  
Vol 20 (1) ◽  
pp. 435 ◽  
Author(s):  
Jieyun Cao ◽  
Michael Ng ◽  
Melanie A Felmlee
Keyword(s):  
Sex Differences ◽  
Membrane Protein ◽  
Protein Expression ◽  
Estrous Cycle ◽  
Sex Hormones ◽  
Membrane Trafficking ◽  
Drug Disposition ◽  
Membrane Expression ◽  
Membrane Protein Expression ◽  
Estrous Cycle Stage

Purpose: Monocarboxylate transporters (MCTs) are involved in the transport of monocarboxylates such as ketone bodies, lactate, and pharmaceutical agents. CD147 functions as an ancillary protein for MCT1 and MCT4 for plasma membrane trafficking. Sex differences in MCT1 and MCT4 have been observed in muscle and reproductive tissues; however, there is a paucity of information on MCT sex differences in tissues involved in drug disposition. The objective of the present study was to quantify hepatic MCT1, MCT4 and CD147 mRNA, total cellular and membrane protein expression in males, over the estrous cycle in females and in ovariectomized (OVX) females. Method: Liver samples were collected from females at the four estrous cycle stages (proestrus, estrus, metestrus, diestrus), OVX females and male Sprague-Dawley rats (N = 3 – 5). Estrus cycle stage of females was determined by vaginal lavage. mRNA and protein (total and membrane) expression of MCT1, MCT4 and CD147 was evaluated by qPCR and western blot analysis. Results: MCT1 mRNA and membrane protein expression varied with estrous cycle stage, with OVX females having higher expression than males, indicating that female sex hormones may play a role in MCT1 regulation. MCT4 membrane expression varied with estrous cycle stage with expression significantly lower than males. MCT4 membrane expression in OVX females was also lower than males, suggesting that androgens play a role in membrane expression of MCT4. Males had higher membrane CD147 expression, whereas there was no difference in whole cell protein and mRNA levels suggesting that androgens are involved in regulating CD147 membrane localization. Conclusions: This study demonstrates hepatic expression and membrane localization of MCT1, MCT4 and CD147 are regulated by sex hormones. Sex differences in hepatic MCT expression may lead to altered drug disposition, so it is critical to elucidate the underlying mechanisms in the sex hormone-dependent regulation of MCT expression. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals A Region Within the Third Extracellular Loop of Rat AQP6 Precludes Its Trafficking to Plasma Membrane in a Heterologous Cell Line.

2021 ◽  
Author(s):  
David Soler ◽  
Thomas Kowatz ◽  
Andrew Sloan ◽  
Thomas McCormick ◽  
Kevin Cooper ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plasma Membrane ◽  
Membrane Protein ◽  
Cell Line ◽  
Membrane Trafficking ◽  
Extracellular Loop ◽  
Reporter System ◽  
The Third ◽  
Heterologous Cell ◽  
Retention Signal

Abstract The inability to over-express AQP6 in the plasma membrane of heterologous cells has hampered efforts to further characterize the function of this aquaglyceroporin membrane protein at atomic detail. Using the AGR reporter system we have identified a region within loop C of AQP6 that is responsible for severely hampering its plasma membrane localization. Serine substitution corroborated that amino acids present within AQP6194-213 of AQP6 loop C contribute to intracellular retention. This intracellular retention signal may preclude proper plasma membrane trafficking and severely curtail expression of AQP6 in heterologous cells.

Patching plasma membrane disruptions with cytoplasmic membrane

2000 ◽  
Vol 113 (11) ◽  
pp. 1891-1902 ◽  
Author(s):  
P.L. McNeil ◽  
S.S. Vogel ◽  
K. Miyake ◽  
M. Terasaki
Keyword(s):  
Plasma Membrane ◽  
Membrane Protein ◽  
Sea Urchin ◽  
Membrane Trafficking ◽  
Cytoplasmic Membrane ◽  
Yolk Granule ◽  
High Threshold ◽  
Surface Markers ◽  
Plasma Membrane Protein ◽  
Cell Cytoplasm

Vesicle-vesicle fusion initiated in cell cytoplasm by high Ca(2+) can rapidly erect large membrane boundaries. These might be used as a ‘patch’ for resealing plasma membrane disruptions. Three central predictions of this ‘patch’ hypothesis are here established in sea urchin eggs. First, we show that surface markers for plasma membrane protein and lipid are initially absent over disruption sites after resealing is complete. Second, we demonstrate that resealing capacity is strongly dependent upon local availability of fusion competent cytoplasmic organelles, specifically the reserve or yolk granule. Lastly, we demonstrate that the reserve granule is capable of rapid (t(1/2) <1 second), Ca(2+)-regulated (high threshold) fusion capable of erecting large (>1000 μm(2)), continuous membrane boundaries. Production of patch vesicles for resealing may proceed by an ‘emergency’ fusion mechanism distinct from that utilized for the much slower, highly regulated, cytosol-requiring organelle-organelle fusion events typical of constitutive membrane trafficking pathways.

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