Multiple routes of chemosensitivity to free fatty acids in humans

2007 ◽  
Vol 292 (5) ◽  
pp. G1206-G1212 ◽  
Author(s):  
Angela Chalé-Rush ◽  
John R. Burgess ◽  
Richard D. Mattes
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Sensory Systems ◽  
Forced Choice ◽  
Lower Sensitivity ◽  
Detection Thresholds ◽  
Olfactory Stimuli ◽  
Multiple Routes ◽  
Presentation Procedure

Selected free fatty acids (FFAs) are documented effective somatosensory and olfactory stimuli whereas gustatory effects are less well established. This study examined orthonasal olfactory, retronasal olfactory, nasal irritancy, oral irritancy, gustatory, and multimodal threshold sensitivity to linoleic, oleic, and stearic acids. Sensitivity to oxidized linoleic acid was also determined. Detection thresholds were obtained using a three-alternative, forced-choice, ascending concentration presentation procedure. Participants included 22 healthy, physically fit adults sensitive to 6- n-propylthiouracil. Measurable thresholds were obtained for all FFAs tested and in 96% of the trials. Ceiling effects were observed in the remaining trials. Greater sensitivity was observed for multimodal stimulation and lower sensitivity for retronasal stimulation. There were no statistically significant correlations for linoleic acid thresholds between different modalities, suggesting that each route of stimulation contributes independently to fat perception. In summary, 18-carbon FFAs of varying saturation are detected by multiple sensory systems in humans.

scholarly journals Potential Interference by Free Fatty Acids in Determination of Tricyclazole in Brown Rice by Gas Chromatography with Flame Thermionic Detection

1996 ◽  
Vol 79 (6) ◽  
pp. 1471-1476
Author(s):  
Yukari Tsumura ◽  
Yumiko Nakamura ◽  
Yasuhide Tonogai ◽  
Tadashi Shibata
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Brown Rice ◽  
Peak Height ◽  
Silica Cartridge ◽  
Limit Of Quantitation ◽  
First Time

Abstract A convenient method is described for the determination of tricyclazole in brown rice, and the interference of free fatty acids with flame thermionic detection (FTD) is reported for the first time. Brown rice is extracted with acetone, the extract is filtered, and the filtrate is evaporated. To the residue is added 10% (w/v) NaCI solution, and the mixture is extracted with ethyl acetate. The extract is charged on a Sep-Pak Plus silica cartridge. Free fatty acids are removed from the rice by washing with diethyl ether, and tricyclazole is eluted with acetone-n-hexane (1 + 1). Tricyclazole is determined on a DB-1 capillary column by gas chromatography with FTD (GC–FTD). Linoleic acid and oleic acid, which have essentially the same retention time as tricyclazole, cannot be detected by FTD. Thus, without the Sep- Pak Plus silica cleanup, the peak height of tricyclazole in the chromatogram decreased, the extent depending on the concentration of linoleic acid, n-Hexane–acetonitrile partitioning was not used for cleanup because it could not remove 50% of the free fatty acids. Recoveries (mean ± standard deviation, n = 5) of tricyclazole from rice fortified at 2 and 0.1 ppm were 90.5 ±9.4% and 81.3±10.6%, respectively. The limit of quantitation was 0.05 ppm.

scholarly journals Observations on the pattern on biohydrogenation of esterified and unesterified linoleic acid in the rumen

1974 ◽  
Vol 31 (1) ◽  
pp. 99-108 ◽  
Author(s):  
R. C. Noble ◽  
J. H. Moore ◽  
C. G. Harfoot
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Free Acid ◽  
Octadecenoic Acid ◽  
Conjugated Diene ◽  
Plasma Triglycerides ◽  
Fatty Acid Compositions

1. Studies have been made of the effects of different concentrations of either free or esterified linoleic acid on the biohydrogenation of linoleic acid by rumen micro-organisms in vitro. A comparison has been made with the changes which occurred in the fatty acid compositions of rumen free fatty acids and plasma triglycerides of sheep given intraruminal infusions of linoleic acid or maize oil.2. In the in vitro experiments, with increasing concentrations of 18:2 added as the free fatty acid, a decreasing proportion of this 18:2 was hydrogenated to 18:0 andtrans-11-octadecenoic acid accumulated. The accumulation of large amounts oftrans-11-octadecenoic acid was accompanied in all instances by the accumulation of a conjugated diene identified ascis-9,trans-11-octadecadienoic acid. There appeared to be a product–precursor relationship between the conjugated diene and thetrans-11 monoene.3. When linoleic acid was presented in vitro as the triglyceride, the extent to which hydrogenation occurred was, in all instances, greater than when equivalent amounts of 18:2 were presented as the free acid. Only small amounts of thecis-9,trans-11 diene were detected, and there was no apparent product–precursor relationship between this conjugated diene and the C18monoenoic acids. The C18monoenoic acids that accumulated consisted of bothcisandtransisomers; thecisisomers consisted largely ofcis-9- andcis-11-octadecenoic acids, which together comprised about 30% of the C18monoenoic acids present.4. The infusion of free linoleic acid into the rumen of sheep resulted in an increase in the proportion of total 18:1 and a decrease in the proportions of 16:0 and 18:0 in the total rumen free fatty acids. This increase which occurred in the concentration of 18:1 consisted predominantly of thetrans-11 isomer. A concomitant increase in the concentration of the C18trans-11 acid was observed to occur in the fatty acids of the plasma triglycerides. Infusion of maize oil into the rumen of sheep resulted in little change in the fatty acid compositions of either the free fatty acids in the rumen or the triglycerides of the plasma.5. The findings in vitro and in vivo are discussed with reference to each other and with reference to the possibility that biohydrogenation of 18:2 derived from the triglyceride proceeds by a different pathway from that of 18:2 presented as the free acid.

Free fatty acids in human cerebrospinal fluid following subarachnoid hemorrhage and their potential role in vasospasm: a preliminary observation

2002 ◽  
Vol 97 (2) ◽  
pp. 272-279 ◽  
Author(s):  
Julie G. Pilitsis ◽  
William M. Coplin ◽  
Michael H. O'Regan ◽  
Jody M. Wellwood ◽  
Fernando G. Diaz ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cerebrospinal Fluid ◽  
Linoleic Acid ◽  
Subarachnoid Hemorrhage ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Potential Role ◽  
The Other ◽  
Content Type ◽  
Fine Print

Object. The mechanisms leading to vasospasm following subarachnoid hemorrhage (SAH) remain unclear. Accumulation in cerebrospinal fluid (CSF) of free fatty acids (FFAs) may play a role in the development of vasospasm; however, in no previous study have concentrations of FFAs in CSF been examined after SAH. Methods. We collected samples of CSF from 20 patients with SAH (18 cases of aneurysmal SAH and two cases of spontaneous cryptogenic SAH) and used a high-performance liquid chromatography assay to determine the FFA concentrations in these samples. We then compared these findings with FFA concentrations in the CSF of control patients. All FFA concentrations measured 24 hours after SAH were significantly greater than control concentrations (p < 0.01 for palmitic acid and < 0.001 for all other FFAs). All measured FFAs remained elevated for the first 48 hours after SAH (p < 0.05 for linoleic acid, p < 0.01 for palmitic acid, and p < 0.001 for the other FFAs). After 7 days, a second elevation in all FFAs was observed (p < 0.05 for linoleic acid, p < 0.01 for palmitic acid, and p < 0.001 for the other FFAs). Samples of CSF collected within 48 hours after SAH from patients in whom angiography and clinical examination confirmed the development of vasospasm after SAH were found to have significantly higher concentrations of arachidonic, linoleic, and palmitic acids than samples collected from patients in whom vasospasm did not develop (p < 0.05). Conclusions. Following SAH, all FFAs are initially elevated. A secondary elevation occurs between 8 and 10 days after SAH. This study provides preliminary evidence of FFA elevation following SAH and of a potential role for FFAs in SAH-induced vasospasm. A prospective study is warranted to determine if CSF concentrations of FFAs are predictive of vasospasm.

scholarly journals Variations in fatty acid composition and oxidative stability of hazelnut (Corylus avellana L.) varieties stored by traditional method

2019 ◽  
Vol 70 (1) ◽  
pp. 288 ◽  
Author(s):  
H. Karaosmanoğlu ◽  
N. Ş. Üstün
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Storage Period ◽  
Peroxide Number ◽  
External Interference

In this study, the changes in fatty acid composition, peroxide number, free fatty acids, oleic acid/ linoleic acid (O/L) and iodine value (IV) were investigated during the traditional storage of hazelnuts. The samples were selected from Giresun Quality Tombul, Kara and Sivri hazelnut varieties with economical prescription. Samples were stored according to the conventional methods in external interference-free warehouses until the next harvest time. At the end of storage, the amount of oleic acid in all varieties increased while the amount of linoleic acid decreased. Even though an increase in the free fatty acids and peroxide number in all types of hazelnuts during storage was determined, the values were considerably lower than the rancidity limits at the end of the storage period. As a result of the study it was observed that the hazelnut shell is an important preservative during storage and that hazelnuts can be preserved until the next harvest period under simple storage conditions.

Distribution of androstenedione and its effects on total free fatty acids in pregnant rats

2007 ◽  
Vol 23 (2) ◽  
pp. 65-74 ◽  
Author(s):  
C.S. Kim ◽  
I.A. Ross ◽  
R.L. Sprando ◽  
W.D. Johnson ◽  
S.C. Sahu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Corn Oil ◽  
Fetal Brain ◽  
Maternal Blood ◽  
Maternal Plasma ◽  
Female Rats ◽  
Pregnant Rats ◽  
Target Organs

Androstenedione, an anabolic steroid used to enhance athletic performance, was administered in corn oil by gastric intubation once daily in the morning to nonpregnant female rats at a dose of 5 or 60mg/kg/day, beginning two weeks before mating and continuing through gestation day (GD) 19. On GD 20, the distribution of androstenedione and other steroid metabolites was investigated in the maternal plasma and target organs, including brain and liver. The concentration of estradiol in plasma approached a statistically significant increase after treatment as compared with the controls, whereas the levels of androstenedione, testosterone and progesterone were not significantly different from the controls. In the liver, the concentrations of androstenedione and estradiol only were increased in a dose-related manner. None of these steroids was detectable in the brain. Androstenedione treatment also produced changes in the level of selected free fatty acids (FFAs) in the maternal blood, brain, liver and fetal brain. The concentrations of palmitic acid (16:0) and stearic acid (18:0) in the plasma were not significantly different between the controls and treated rats. However, oleic acid (18:1), linoleic acid (18:2) and docosahexaenoic acid (DHA, 22:6) were 17.94 ± 2.06 μg/ml, 24.23 ± 2.42 μg/ml and 4.08 ± 0.53 μg/ml, respectively, in the controls, and none of these fatty acids was detectable in the treated plasma. On the other hand, palmitic, stearic, oleic, linoleic and DHA were present in both control and treated livers. Among the FFAs in liver, linoleic and DHA were increased 87% and 169%, respectively, over controls. Palmitic, stearic and oleic acids were not significantly affected by the 60 mg/kg treatment. These were present in both control maternal and fetal brains, whereas linoleic acid was found only in fetal brain control. DHA was present only in the control maternal brain (0.02 ± 0.02 μg/mg protein) and fetal brain (0.24 ± 0.15 μg/mg protein). The results indicated that androstenedione exhibits significantly different effects on the FFA composition among target organs during pregnancy.

Metabolism of Palmitic and Linoleic Acids in Man: Differences in Turnover and Conversion to Glycerides

1971 ◽  
Vol 40 (4) ◽  
pp. 345-350 ◽  
Author(s):  
P. J. Nestel ◽  
P. Barter
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Palmitic Acid ◽  
Free Fatty Acids ◽  
Plasma Triglyceride ◽  
Turnover Rates ◽  
Polyunsaturated Fat ◽  
Total Turnover ◽  
Fat Diets

1. The metabolism of palmitic acid (a saturated fatty acid) and linoleic acid (a polyunsaturated fatty acid) was compared in seven subjects during constant infusions of the radioactive tracers. 2. The studies were repeated in some subjects after the turnover of the free fatty acids and the size of the fatty acid and glyceride pools had been altered with sucrose or polyunsaturated fat diets. 3. The fractional turnover of linoleic acid was nearly always greater than that of palmitic acid, though its total turnover rate was less. 4. A lesser proportion of the turnover of linoleate than of palmitate was incorporated into plasma triglyceride over a range of turnover rates of free fatty acids and glyceride fatty acid pools. This may be a factor in the lowering of plasma triglyceride concentrations with diets rich in polyunsaturated fatty acids.

Effects of free fatty acids on the organization of cytoskeletal elements in lymphocytes

1981 ◽  
Vol 1 (10) ◽  
pp. 939-948
Author(s):  
R L Hoover ◽  
K Fujiwara ◽  
R D Klausner ◽  
D K Bhalla ◽  
R Tucker ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Surface Membrane ◽  
Colchicine Treatment ◽  
Surface Receptors ◽  
Organizing Center ◽  
Entire Cell ◽  
Cytoskeletal Elements ◽  
Mouse Lymphocytes

Treatment of mouse lymphocytes with cis-unsaturated free fatty acids produced alterations in the immunofluorescence patterns of the cytoskeleton and contractile proteins. Saturated free fatty acids and trans-unsaturated free fatty acids had no effect. In untreated cells, the microtubular pattern exhibited radiation from an organizing center, resembling the spokes of an umbrella. The addition of linoleic acid produced a polarized submembranous aggregate. Under control conditions, staining for actin revealed a diffuse pattern over the entire cell, but the addition of linoleic acid caused the formation of a single large patch, or polarized submembranous aggregate. The pattern for alpha-actinin normally revealed intense perinuclear staining on a diffuse background. Linoleic acid caused the loss of this pattern and the formation of a polarized submembranous aggregate. Linoleic acid treatment also caused the pattern for myosin to change from diffuse to uniform submembranous patching around the periphery of the cell. For all of these proteins, calcium (8 mM), but not magnesium, partially reversed the effects of linoleic acid. Sodium azide had little effect on the normal distribution of actin, tubulin, and alpha-actinin; however, myosin staining revealed prominent patch formation. Colchicine treatment caused diffuse staining, some polarized submembranous aggregate formation of tubulin, and some patching of myosin, but not as extensively as did treatment with linoleic acid. Actin and alpha-actinin were unaffected. These results, in view of the previously shown facts that pretreatment of cells with linoleic acid followed by anti-immunoglobulin inhibits capping of surface immunoglobulin (Klausner, et al., Proc. Natl. Acad. Sci. U.S.A. 77:437-441, 1980) and that free fatty acids partition into the surface membrane (Klausner et al., J. Biol. Chem. 255:1286-1295, 1980), suggest that the perturbation of the plasma membrane with unsaturated free fatty acids alters the interaction of surface receptors with the cytoskeleton, which in turn affects cytoplasmic distribution of the proteins.

The role of Lathyrus sativus flower surface wax in short-range attraction and stimulant for nymph laying by an adult viviparous aphid

2019 ◽  
Vol 110 (2) ◽  
pp. 231-241 ◽  
Author(s):  
Paroma Mitra ◽  
Syed Husne Mobarak ◽  
Rahul Debnath ◽  
Anandamay Barik
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Short Range ◽  
Lathyrus Sativus ◽  
Tridecanoic Acid ◽  
Surface Wax ◽  
Important Pest ◽  
Hexane Extracts ◽  
Synthetic Blend

AbstractAphis craccivora Koch (Hemiptera: Aphididae) is an important pest of Lathyrus sativus L. plants, and causes retarded plant growth and loss of seed production. The insect sucks cell sap from flowers and lays nymphs on flowers. Hence, an attempt has been made to observe whether flower surface wax compounds (alkanes and free fatty acids) from two cultivars (BIO L 212 Ratan and Nirmal B-1) of L. sativus could act as short-range attractant and stimulant for nymph laying by adult viviparous females. The n-hexane extracts of flower surface waxes were analyzed by TLC, GC-MS and GC-FID analyses. Twenty one and 22 n-alkanes between n-C12 and n-C36 were detected in BIO L 212 Ratan and Nirmal B-1, respectively; whereas 12 free fatty acids between C12:0 and C22:0 were identified in both cultivars. Pentadecane and tridecanoic acid were predominant n-alkane and free fatty acid, respectively. One flower equivalent surface wax of both cultivars served as short-range attractant and stimulant for nymph laying through Y-tube choice experiments and I-tube viviparity assays, respectively, by adult viviparous females. A synthetic blend of nonacosane, tridecanoic acid and linoleic acid, and a synthetic blend of tetradecane, pentadecane, nonacosane, tridecanoic acid and linoleic acid resembling in amounts as present in one flower equivalent surface wax of BIO L 212 Ratan and Nirmal B-1, respectively, served as short-range attractant and stimulant for nymph laying by adult viviparous females. This study suggests that both these blends could be used in lures in the development of baited traps in pest management programmes.

The effect of fatty acids on the developmental direction of Strongyloides ratti first-stage larvae

1989 ◽  
Vol 63 (2) ◽  
pp. 102-106 ◽  
Author(s):  
Takeya Minematsu ◽  
Tatsuyuki Mimori ◽  
Mitsuhiro Tanaka ◽  
Isao Tada
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Free Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Physiological Changes ◽  
Infective Larvae ◽  
Third Stage ◽  
Developmental Course

ABSTRACTThe effect of fatty acids was studied on the developmental direction of Strongyloides ratti first-stage larvae (L1). The proportion of third-stage infective larvae increased markedly when L1 were cultured in faeces with added fatty acids such as palmitic (C16), stearic (C18), oleic (C18:1) and linoleic (C18:2) acids. Unsaturated fatty acids were more effective than saturated ones. Moreover, the proportion of infective larvae increased with quantity of linoleic acid but the triacylglycerols of any fatty acid had no effect. These results suggest that these free fatty acids cause physiological changes that determine the developmental course of L1 of S. ratti in nature.

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