Pharmacology and Phytochemistry of Coriander

Coriander, named as Coriandrum sativum Linn, belongs to the family Umbelliferae and is one of the most popular and well-known spices/condiments and herbal medicines. The essential oils and fatty oils are the two major active chemical constituents present in the plant. The other minor ingredients found to be present are monoterpenes hydrocarbons i-e limonene, γ-terpinene, α-pinene, p-cymene, borneol, citronellol, camphor, geraniol, and geraniol acetate and abd heterocyclic components such as pyrazine, pyridine, thiazole, furan and tetrahudrofuran derivatives, isocoumarins, coriandrin, dihydrocoriandrin, coriandrons A-E, flavonoids. The volatile oil from the leaf contains aromatic acids such as 2-decenoic acid, E-11-tetradecenoic acid, undecyl alcohol, tridecanoic acid, capric acid, undecanoic acid, and more. The current pharmacological research reveals the application of coriander has antibacterial and antifungal activity.

Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep

HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fat-tailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.

HEXANE SOLUBLE BIOACTIVE COMPONENTS OF Chenopodium murale STEM

Chenopodium murale L. is a common winter weed mostly growing along the road-sides. In the present study, n-hexane soluble fraction of methanolic stem extract of this weed was subjected to GC-MS analysis that showed the presence of 28 compounds in this fraction. Major compounds included oleic acid (16.55%), palmitic acid (11.22%), β-sitosterol (9.63%), hexadecanoic acid (7.71%) and methyl oleate (5.90%). Other prominent compounds were piperine (4.75%), nonacosane (4.69%), monoplalmitin (4.21%), γ-sitosterol (3.91%), methyl linoleate (3.88%), neocurdione (3.86%) and ethanonone (3.25%). The compounds such as stigmasterol (2.92%), 1,4-benzenedicarboxylic acid (2.78%), tetracosanoic acid (1.19%), stearic acid (1.36%), tridecanoic acid (1.35%), tridecanal (1.30%), phytol (1.29%), docosanoic acid, methyl ester (1.23%), octadecanoic acid (1.20%), 4-pyrimidinecarboxylic acid (1.05%), 2-hydroxy-2-phenylbutanamide (0.91%), hexacosanoic acid (0.76%), cholestrol (0.61%), methyl octacosanoate (0.55%) and tetracosanoic acid (0.30%) were present in low concentrations. A thorough literature survey showed that most of the identified compounds possessed antifungal and/or antibacterial properties while very few of them also possessed antioxidant potential. This study concludes that n-hexane soluble fraction of methanolic stem extract of C. murale is a big storehouse of antimicrobial compounds.

The Association of Free Fatty Acids and Eicosanoids with the Severity of Depressive Symptoms in Stroke Patients

The study was designed to demonstrate the relationship of free fatty acids (FFAs) and eicosanoids levels with the severity of depressive symptoms in stroke. The ischemic stroke patients (n = 74) were included in the prospective study. The risk of depression was evaluated by the Beck Depression Inventory-II (BDI-II) 7 days and 6 months after the stroke onset. FFAs and inflammatory metabolites were determined by gas chromatography and liquid chromatography. In the acute phase of stroke, BDI-II and FFAs inversely correlated with C13:0 tridecanoic acid, C15:1 cis-10-pentadecanoid acid, C17:1 cis-10- heptadecanoid acid, C18:0 stearic acid, C20:3n6 eicosatrienoic acid, C22:1cis13 docosenoic acid and C22:6n3 docosahexaenoic acid (DHA). DHA level was significantly lower in patients with low vs. high BDI-II score. In the follow-up examination, BDI-II score directly correlated with C16:0 palmitic acid. The changes in BDI-II score during 6-month observation inversely correlated with lipoxin A4 and protectin D1, and directly correlated with 5-oxo-ETE. Importantly, the severity of depressive symptoms was associated with n3 PUFA level. Diet-derived FFAs were observed to potentially affect the inflammatory pathways in pathogenesis of depression in stroke and reduced DHA levels can attenuate depressive symptoms in stroke patients.

Potensi Ekstrak Daun Dewandaru (Eugenia uniflora L.) Sebagai Pengendali Bakteri Bacillus cereus ATCC 11778 Penyebab Diare

Eugenia uniflora L. merupakan tanaman obat yang dapat dimanfaatkan sebagai obat diare. Eksplorasi senyawa antibakteri dari bahan alam dapat mengatasi berbagai penyakit infeksi yang tidak menimbulkan efek resistensi yang berat seperti penggunaan antibiotika. Penelitian bertujuan mengetahui potensi ekstrak daun dewandaru sebagai pengendali bakteri Bacillus cereus ATCC 11778 penyebab diare dan mengetahui senyawa aktif yang terkandung dalam ekstrak daun dewandaru. Metode yang digunakan dalam penelitian adalah Rancangan Acak Lengkap (RAL) dengan 7 perlakuan konsentrasi ekstrak daun dewandaru yaitu 1%, 2%, 3%, 4%, 5% (b/v), kontrol positif (amoxicillin), dan kontrol negatif (etanol). Penentuan daya hambat ekstrak daun dewandaru terhadap B. cereus ATCC 11778 ditandai dengan terbentuknya daerah bening disekitar kertas cakram menggunakan metode Kirby Baurer. Penelitian menunjukkan bahwa pemberian ekstrak etanol daun dewandaru pada konsentrasi tertinggi (5%) mampu menghasilkan diameter zona hambat sebesar 7,15 mm terhadap B. cereus ATCC 11778. Hasil uji fitokimia menunjukkan ekstrak daun dewandaru mengandung senyawa tannin, saponin, terpenoid, dan flavonoid. Analisis GCMS terhadap fraksi aktif ekstrak daun dewandaru (Fraksi II) menghasilkan sepuluh senyawa yang bersifat antibakteri. Ekstrak etanol daun dewandaru mampu menghambat pertumbuhan B. cereus ATCC 11778 secara in vitro. Konsentrasi ekstrak daun dewandaru 1% merupakan konsentrasi minimal sebagai antibakteri B. cereus ATCC 11778. Terdapat 10 senyawa aktif sebagai antibakteri B. cereus ATCC 11778 yang terkandung dalam ekstrak daun dewandaru, diantaranya Dodecanoic acid, Tridecanoic acid, Hexadecanoic acid, Octadecanoid acid, 8-Nitro-11-dodecanolide, Oxiraneoctanoic acid, Oxiraneoctanoic acid, 9-Octadecenoic acid, 9-Octadecenoic acid, dan 1.2-Benzenedicarboxylic acid. Kata kunci: Antibakteri, Zona hambat, Senyawa Aktif.

Pharmacology and Phytochemistry of Coriander

Coriander, named as Coriandrum sativum Linn, belongs to the family Umbelliferae and is one of the most popular and well-known spices/condiments and herbal medicines. The essential oils and fatty oils are the two major active chemical constituents present in the plant. The other minor ingredients found to be present are monoterpenes hydrocarbons i-e limonene, γ-terpinene, α-pinene, p-cymene, borneol, citronellol, camphor, geraniol, and geraniol acetate and abd heterocyclic components such as pyrazine, pyridine, thiazole, furan and tetrahudrofuran derivatives, isocoumarins, coriandrin, dihydrocoriandrin, coriandrons A-E, flavonoids. The volatile oil from the leaf contains aromatic acids such as 2-decenoic acid, E-11-tetradecenoic acid, undecyl alcohol, tridecanoic acid, capric acid, undecanoic acid, and more. The current pharmacological research reveals the application of coriander has antibacterial and antifungal activity.

The role of Lathyrus sativus flower surface wax in short-range attraction and stimulant for nymph laying by an adult viviparous aphid

Aphis craccivora Koch (Hemiptera: Aphididae) is an important pest of Lathyrus sativus L. plants, and causes retarded plant growth and loss of seed production. The insect sucks cell sap from flowers and lays nymphs on flowers. Hence, an attempt has been made to observe whether flower surface wax compounds (alkanes and free fatty acids) from two cultivars (BIO L 212 Ratan and Nirmal B-1) of L. sativus could act as short-range attractant and stimulant for nymph laying by adult viviparous females. The n-hexane extracts of flower surface waxes were analyzed by TLC, GC-MS and GC-FID analyses. Twenty one and 22 n-alkanes between n-C12 and n-C36 were detected in BIO L 212 Ratan and Nirmal B-1, respectively; whereas 12 free fatty acids between C12:0 and C22:0 were identified in both cultivars. Pentadecane and tridecanoic acid were predominant n-alkane and free fatty acid, respectively. One flower equivalent surface wax of both cultivars served as short-range attractant and stimulant for nymph laying through Y-tube choice experiments and I-tube viviparity assays, respectively, by adult viviparous females. A synthetic blend of nonacosane, tridecanoic acid and linoleic acid, and a synthetic blend of tetradecane, pentadecane, nonacosane, tridecanoic acid and linoleic acid resembling in amounts as present in one flower equivalent surface wax of BIO L 212 Ratan and Nirmal B-1, respectively, served as short-range attractant and stimulant for nymph laying by adult viviparous females. This study suggests that both these blends could be used in lures in the development of baited traps in pest management programmes.