Increased aortic PGI2 and plasma lyso-PAF in the unclipped one-kidney hypertensive rat

1986 ◽  
Vol 251 (6) ◽  
pp. H1361-H1364 ◽  
Author(s):  
H. M. McGowan ◽  
R. Vandongen ◽  
J. P. Codde ◽  
K. D. Croft
Keyword(s):  
Blood Pressure ◽  
Hydrolysis Product ◽  
Platelet Activating Factor ◽  
Hypertensive Rats ◽  
Hypertensive Rat ◽  
Group A ◽  
The One ◽  
Group B ◽  
Group D

Previous studies have implicated vasodilatory prostaglandins (PGs) in the reversal of hypertension following unclipping in the one-kidney, one-clip (1K,1C) hypertensive rat. The capacity of the aorta to synthesize prostacyclin (PGI2) was compared in clipped (group A, n = 9), unclipped (group B, n = 8 and group D, n = 9), and sham-unclipped (group C, n = 9) 1K,1C hypertensive rats. The involvement of platelet-activating factor (PAF), a potent renal antihypertensive phospholipid, in the reversal of renal clip hypertension was also examined. Hypertensive rats [systolic blood pressure (BP) greater than 180 mmHg] were fed a synthetic diet for 4 wk, after which group A was killed immediately, group C was sham-unclipped, and groups B and D unclipped and killed 24 h later. Blood was drawn for the measurement of plasma lyso-PAF (the precursor of PAF) and the aorta removed for determination of 6-ketoprostaglandin F1 alpha (6-keto-PGF1 alpha, the stable hydrolysis product of PGI2). BP fell substantially in the unclipped rats (groups B and D) but did not change in the sham-unclipped rats (group C). Mean aortic 6-keto-PGF1 alpha was increased in the unclipped groups [group B, 15.4 +/- 2.4 (SE) ng/mg; group D, 10.8 +/- 2 ng/mg] compared with group A (7.7 +/- 1 ng/mg) and group C (7.1 +/- 1 ng/mg) (H = 13.74, P less than 0.01). Plasma lyso-PAF was also significantly increased in the unclipped (group D, 261 +/- 26 ng/ml) vs. the sham-unclipped group (group C, 211 +/- 23 ng/ml, P less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Hematological Features in Sheep with IgG and IgM Antibodies against Borrelia burgdorferi sensu lato

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 164
Author(s):  
Labrini V. Athanasiou ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Panagiotis D. Katsoulos
Keyword(s):  
Borrelia Burgdorferi ◽  
Rural Areas ◽  
Blood Analysis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Igm Antibodies ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Immunofluorescence Antibody

Exposure of sheep to Borreliaburgdorferi sensulato (s.I.) complex, the causative agent of Lyme borreliosis (LB), has been reported in tick-abundant areas worldwide, while no data have been reported in Greece. The aim of the study was to identify the hematological alterations in sheep with seropositivity against Borrelia burgdorferi (s.I.). Blood samples were obtained from 318 tick infested sheep for blood analysis and serological determination of IgG and IgM antibodies against B. burgdorferi by indirect immunofluorescence antibody (IFA) assay after exclusion of endo-ectoparasites and other tick-borne infections. A total number of 162 sheep met the inclusion criteria, allocated in four groups based on the presence or absence of IgG and/or IgM; sheep found negative for IgM and IgG (Group A), positive for IgM (Group B), positive for both IgM and IgG (Group C) and positive for IgG (Group D). Anemia, thrombocytopenia and normal or decreased leukocyte count, mainly due to lymphopenia were the main hematological features observed in seropositive sheep. The presence of these features raises the suspicion of Borrelia infection in tick infested sheep. The seropositivity of 23.58% in sheep raises concerns of Borrelia circulation, especially in rural areas and potential risk of transmission to humans.

Abstract 15439: Lesion Complexity and Long-term Outcomes According to Mean Observed Blood Pressure After Percutaneous Coronary Intervention

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Jong Il Park ◽  
Byung Jun Kim ◽  
Hun Tae Kim ◽  
Jung Hee Lee ◽  
Ung Kim ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Percutaneous Coronary Intervention ◽  
Independent Predictor ◽  
Coronary Intervention ◽  
Long Term Outcomes ◽  
Percutaneous Coronary ◽  
Group A ◽  
Group B ◽  
Group D

Background: The guideline recommended strict blood pressure (BP) control for the patients treated with percutaneous coronary intervention (PCI). We aimed to evaluate the relationship between mean observed BP and long-term outcomes for patients with or without lesion complexity. Methods: A total of 1,470 patients who underwent PCI were investigated. We categorized the study population into four groups based on mean observed BP and lesion complexity (left main & three-vessel disease, chronic total occlusion, total stent length ≥60mm, or bifurcation two stenting): Group A (non-complex & systolic BP ≤120mmHg, n=310), Group B (non-complex & systolic BP >120mmHg, n=674), Group C (complex & systolic BP ≤120mmHg, n=131), and Group D (complex & systolic BP >120mmHg, n=355). We evaluated major adverse cardiovascular and cerebrovascular events (MACCEs), defined as cardiac death, myocardial infarction (MI), repeat target vessel revascularization, or stroke. Results: Well-controlled BP group (Group A&C) showed significantly lower systolic BP than uncontrolled BP group (Group B&D) (114.3±6.1mmHg vs. 130.7±7.6mmHg, p<0.001). MACCEs occurred less frequently in Group A (18.9%) than Group B (23.6%), Group C (22.7%), and Group D (33.6%) (p=0.001) at 8 years. After multivariate analysis, with Group A as a reference, the adjusted hazard ratio (HR) for MACCEs was 1.382 (95% confidence interval [CI] 0.978-1.954, p=0.067) for Group B, 1.559 (95% CI 0.957-2.540, p=0.075) for Group C, and 1.872 (95% CI 1.296-2.705, p=0.001) for Group D. In Cox regression model, although lesion complexity was not associated with MACCE, systolic BP≤120mmHg was an independent predictor for reduced rate of MACCE (HR 0.667, 95% CI 0.485-0.918, p=0.013). Conclusions: Mean observed systolic BP ≤120mmHg after PCI was independent predictor for reduced MACCEs regardless of lesion complexity. Key Words: Blood Pressure; Percutaneous Coronary Intervention; Lesion Complexity; Treatment Outcome

scholarly journals Effects of Dried Aloe Vera Gel and Diclofenac on Sodium and Potassium Homeostasis: An Experimental Study on Hypertensive Rats

2021 ◽  
Vol 10 (3) ◽  
pp. 133-139
Author(s):  
Nadeem Yaqoob ◽  
Abdul Qudoos Arain ◽  
Mufakhara Fatimah ◽  
Samina Kausar ◽  
Sadia Chiragh
Keyword(s):  
Experimental Study ◽  
Serum Potassium ◽  
Aloe Vera ◽  
Control Group ◽  
Hypertensive Rats ◽  
Aloe Vera Gel ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Sodium And Potassium

Background: Anti-inflammatory role of Aloe vera gel is well established. Diclofenac is extensively used for acute and chronic inflammation. The present study was conducted to compare dried Aloe vera gel and diclofenac effects on sodium and potassium balance in hypertensive rats. Material and Methods: This experimental study was conducted at Sargodha Medical College from May to November 2016. Twenty-four healthy male Sprague Dawley rats 7-8 weeks of age were included in study. Any unhealthy-looking rat was excluded from the study. Rats were equally and randomly divided into four groups Normal control (group A), Model control (group B), Aloe vera (group C) & Diclofenac (group D). Hypertension was induced by a 20 % sucrose diet in all groups except group A in 8 weeks’ time. Group B, C & D received distilled water and Aloe vera dried gel 400 mg/kg & diclofenac powder 12 mg/kg body weight respectively orally between 8 to 10 weeks. Serum and urine analysis was performed for hematocrit, sodium, and potassium concentrations at zero, eight and ten weeks. Twenty-four-hour urinary sodium excretion was calculated. Data was analyzed using Graph Pad Prism version 6. Result: After 2-week administration of aloe vera and diclofenac powder, serum potassium significantly decreased in Group C (p <0.001) while increased in Group B and D (p <0.001) as compared to group A. Urinary sodium concentration and excretion increased significantly in Group C (p <0.01) as compared to Group A whereas result of Group D was insignificant. No significant change in serum sodium and hematocrit of any group was observed. Conclusion: Aloe vera causes less sodium retention than diclofenac but decreases serum potassium contrary to the effect of diclofenac in hypertensive rats.

scholarly journals Impact of anti-inflammatory agent like Bryophyllum pinnatum aqueous extract and diclofenac on blood pressure and creatinine clearance.

2020 ◽  
Vol 27 (10) ◽  
pp. 2110-2116
Author(s):  
Farwa Naqvi ◽  
Iram Imran ◽  
Bilal Habib ◽  
Summyia Sadia ◽  
Zaib ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Creatinine Clearance ◽  
Aqueous Extract ◽  
Control Group ◽  
Inflammatory Agent ◽  
Group A ◽  
Anti Inflammatory ◽  
Group B ◽  
Group D ◽  
Bryophyllum Pinnatum

Objectives: To study the impact of anti-inflammatory agent like Bryophyllum pinnatum aqueous extract and diclofenac on blood pressure and creatinine clearance. Study Design: Experimental study. Setting: Sargodha Medical College, Sargodha, Department of Pharmacy, University of Sargodha. Period: 1st January 2019 to 30th June 2019. Material & Methods: 24 Sprague Dawley rats were obtained and were then divided into four groups. Negative control group (A) contained animals received normal diet while Group B, C and D received diet containing sucrose (20% w/w) to induce hypertension. After that group B (positive control group) received distilled water 0.5 ml was by oral route, group C (Bryophyllum pinnatum group) received Bryophyllum pinnatum aqueous extract 300 mg/ml intraperitoneally and group D received diclofenac 12 mg/kg intraperitoneally as a single morning dose for two weeks. Blood pressure of animals was recorded at baseline and then weekly throughout the study using tail cuff using non-invasive blood pressure controller (ML125R). Animals were anesthetised with chloroform and two ml blood was drawn through cardiac puncture at 0, 4and 6 weeks. Blood was tested for haematocrit. Serum sodium and potassium levels were estimated by flame photometer. Urinary creatinine levels were estimated by kinetic Jaffé method. The data collected was processed by using Statistical Package for Social Sciences (SPSS 20). Results: Mean and standard deviation of systolic blood pressure (BP) of group A rats did not change with time, while that of group B, C and D increased till 4th week. Creatinine clearance of group A, B and C did not change much with time but that of group D decreased towards end of study period. Conclusion: Bryophyllum pinnatum leaf aqueous extract is an effective anti-hypertensive agent with minimal renal effects.

scholarly journals KARAKTERISASI MORFOLOGI DAUN DAN BUNGA BEBERAPA VARIETAS Hoya coronaria DARI KAWASAN HUTAN KERANGAS AIR ANYIR, BANGKA

2018 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Putri Deswanti ◽  
Yulian Fakhrurrozi ◽  
Sri Rahayu
Keyword(s):  
Morphological Characteristics ◽  
Flower Color ◽  
Group A ◽  
Yellow Corolla ◽  
Heath Forest ◽  
Group B ◽  
Munsell Color Chart ◽  
Group D ◽  
Descriptive Method

Hoya coronaria was found in Heath Forest Air Anyir, Bangka in various flower color. Characterization based on morphological characteristics need to clear up the differences between varieties. This research aims to observe morphological variation of six varieties of H. coronaria from Heath Forest Air Anyir, Bangka based on morphology vegetative and flower morphology. The descriptive method was used to describe the morphological character. Determination of color based on Munsell Color Chart for Plant Tissues. The six samples of different varieties was used which has different flower color as follow respectively: yellow corolla with pink corona (var1); yellow corolla with white corona (var2); yellow corolla with pink honey line and pink corona (var3); pink corolla with pink honey line and pink corona (var4); pink corolla with pink honey line and white corona (var5); and white corolla with white corona (var6). The six varieties have different characteristic of vegetative and flower part both on qualitative and quantitative measurement. The cluster analyses resulted two sister groups. The first group (group A) consisted of var3, var4, var5, and var6, and the second group (group B) consisted of var1 and var2. Groups A was consisted of two smaller groups, namely group C and group D. Group C consisted of var3, var4 and var5, while group D consisted of var6

Increased levels of platelet-activating factor (1-O-alkyl-2-acetylglycerophosphocholine) in blood after reversal of renal clip hypertension in the rat

1988 ◽  
Vol 74 (4) ◽  
pp. 393-396 ◽  
Author(s):  
H. M. McGowan ◽  
R. Vandongen ◽  
L. D. Kelly ◽  
K. J. Hill
Keyword(s):  
Blood Pressure ◽  
Whole Blood ◽  
Platelet Activating Factor ◽  
Hypertensive Rats ◽  
Stable Level ◽  
Hypertensive Rat ◽  
Potent Vasodilator ◽  
Cold Acetone

1. This study analyses whole blood in acutely unclipped one-kidney, one-clip (1K,1C) hypertensive rats for the presence of platelet-activating factor (PAF), a potent vasodilator and a putative mediator of the rapid blood pressure (BP) fall seen after unclipping. 2. Hypertensive 1K,1C rats were anaesthetized and a carotid and jugular cannula were inserted for BP measurement and anaesthetic infusion respectively. After a stable level of anaesthesia was attained, the constrictive clip was removed and BP was recorded for 30 min. 3. Blood was drawn from the aorta directly into ice-cold acetone. The extract was analysed for PAF by a bioassay using 5-hydroxy-[14C]tryptamine-labelled platelets. 4. Rats which showed a BP fall had elevated levels of PAF [55 ± 6 (sem) pg/ml] when compared with those with no fall in BP [26 ± 7 (sem) pg/ml] (P < 0.01). 5. This supports the hypothesis that activation of PAF biosynthesis may be a mechanism contributing to the fall in BP seen after unclipping the 1K,1C hypertensive rat.

scholarly journals Effects of calcium carbonate, potassium iodide and zinc sulphate in lead induced toxicities in rat model

1970 ◽  
Vol 4 (2) ◽  
pp. 123-127
Author(s):  
MM Haque ◽  
MA Awal ◽  
M Mostofa ◽  
MMH Sikder ◽  
MA Hossain
Keyword(s):  
Body Weight ◽  
Calcium Carbonate ◽  
Potassium Iodide ◽  
Zinc Sulphate ◽  
The Body ◽  
Postmortem Changes ◽  
Group A ◽  
Group B ◽  
Group D

A study was carried out to find out the effects of calcium carbonate, potassium iodide and zinc sulphate in lead induced toxicities in rat with the observation of toxic signs, postmortem changes and determination of lead quantity in different organs of the body. Twenty-five long Evans rats weighing between 202-305g were randomly divided into five groups keeping group A as untreated control. Rest four groups (B, C, D & E) were treated with lead acetate @ 20mg/kg body weight in addition, rats of groups C, D & E were given Calcium carbonate (50mg/kg body weight), potassium iodide (20mg/kg bwt) and zinc sulphate (10mg/kg bwt.) respectively. Treatments were continued for 42 consecutive days. Rats of group B showed reduction in fecal consumption, anxiety, indigestion, fatigue, muscle tremor, paralysis and ruffled hair coat. However, rats of group C were apparently normal but rats of group D and E showed mild toxic signs of similar nature. On postmortem examination, severe congestion and blackish discoloration with enlargement of the liver, kidney, spleen and inflammation of the gastric mucosa were observed in rats of group B. However, these changes were less severe in other groups. The significant reduction of lead in blood, liver, kidney and brain was observed in rats of group C, D and E in comparison to group B. In addition, significant reduction of lead content was observed in femur of group C only. The present findings revealed that during lead exposure administration of calcium carbonate may be effective in modifying and preventing lead deposition in tissues followed by zinc sulphate and potassium iodide in rat. Key words: Toxicity, lead, calcium carbonate, potassium iodide, zinc sulphate, rat DOI = 10.3329/bjvm.v4i2.1295 Bangl. J. Vet. Med. (2006). 4 (2): 123-127

scholarly journals Assessment of the Cardioankle Vascular Index in Pregnant Women Complicated with Hypertensive Disorders

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Atsushi Yoshida ◽  
Takashi Sugiyama ◽  
Norimasa Sagawa
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Pulse Wave ◽  
Chronic Hypertension ◽  
Significant Difference ◽  
Group A ◽  
The Subject ◽  
The Difference ◽  
Group B ◽  
Group D

It was previously reported that the brachial-ankle pulse wave velocity (baPWV) is elevated in preeclamptic women. However, baPWV is strongly affected by blood pressure. Recently, a new index of vascular stiffness, the cardioankle vascular index (CAVI), was developed. CAVI is thought to be an index independent of blood pressure. We assessed CAVI in normotensive and hypertensive pregnant women. We studied a total of 109 Japanese women consisting of 23 nonpregnant healthy women (group A), 45 normotensive pregnant women (group B), 28 pregnant women complicated with established preeclampsia (group C), and 13 pregnant women with chronic hypertension (group D). The subject remained supine while the blood pressure, baPWV, and CAVI were recorded. No significant difference in baPWV was present between groups C and D, but the difference in CAVI was significantly high in group D. We believe that we can distinguish the vessel structural change between chronic hypertension and preeclampsia through simultaneous baPWV and CAVI measurements.

scholarly journals Comparative efficacy of imported fowl pox virus vaccine with locally produced one in backyard chicks

1970 ◽  
Vol 6 (1) ◽  
pp. 23-26 ◽  
Author(s):  
MR Islam ◽  
MSR Khan ◽  
MA Islam ◽  
MEH Kayesh ◽  
MR Karim ◽  
...  
Keyword(s):  
Virus Vaccine ◽  
Control Group ◽  
Post Vaccination ◽  
History Of ◽  
Group A ◽  
Group B ◽  
Protection And Control ◽  
And Control ◽  
Group D

A total of 80 experimentally reared backyard chicks with the history of non-vaccinated parents to FPV vaccine were used for the determination of persistence of maternally derived antibody in chicks and to compare the efficacy of Poxine® and DLS-FPV vaccine in protecting the chicks. Chicks were divided into five groups namely group A, B, C, D and E where each group contained 15 chicks except group A contained 20 chicks which was also used to determine the persistence of maternally derived antibody (MDA). Birds of group B and C were vaccinated with DLS-FPV at day 22 and 18 respectively through wing web puncture (WWP), whereas group D and E were vaccinated with Poxine® at day 36 and 18 respectively. Sera were randomly colleted from 10 chicks of each group at 7, 14 and 21 days of post vaccination for the determination of antibody titre using PHA test. Ten chickens from each group were challenged three weeks post vaccination with 106 EID50/0.1ml. "Take reaction" was recorded to assess the better immune response in different groups after vaccination. Birds of group B, C, D and E showed 100, 86.67, 93 and 86.67% "take reaction", respectively. The highest Mean PHA titre was found 217.60 ± 19.55 in the chicks of group B. After challenge, the birds of group B and D showed 100% protection, whereas birds of group C and E showed 93.33% protection and control group showed no protection. From the results of PHA test it may be concluded that both Poxine® and DLS-FPV vaccines are equally suitable and the chicks of nonvaccinated origin might be vaccinated with DLS-FPV at day 18 or 22 and 36 in case of Poxine® fowl pox virus vaccine. Key words: Efficacy, fowl pox virus vaccine, backyard chicks, age DOI = 10.3329/bjvm.v6i1.1334 Bangl. J. Vet. Med. (2008). 6 (1): 23-26

scholarly journals Pengaruh Ekstrak Kunyit (Curcuma domestica) dan Meniran (Phyllanthus niruri) Terhadap Profil Darah Ayam

2021 ◽  
Vol 11 (1) ◽  
pp. 6
Author(s):  
Sri Hartati ◽  
Irkham Widiyono ◽  
Slamet Raharjo ◽  
Hary Purnamaningsih ◽  
Alfarisa Nururrozi ◽  
...  
Keyword(s):  
Plasma Protein ◽  
Total Protein ◽  
Control Group ◽  
Blood Profile ◽  
Total Plasma Protein ◽  
Group A ◽  
The One ◽  
Group B ◽  
Group D ◽  
One Way Anova

Abstract  Herbal preparations have started being used to support poultry health. The purpose of this study was to obtain information about the effect of meniran and turmeric extracts as well as the combination of both on the blood profile (hemoglobin, hematocrit, erythrocytes, leukocytes, and plasma protein) of chickens. This study used 80 layer chickens (DOC), which were randomly divided into 4 groups (A, B, C, and D, each group contain 20 chickens). Group A was the control, group B was treated with turmeric extract (100 mg/kg BW), group C was treated with meniran extract (100 mg/kg BW), and group D was treat with a combination of meniran and turmeric extracts (each dose 100 mg/kg BW). Treatment was given for 30 days. Blood samples were collected on the end period for examination. Statistical analysis of the data were done through the one-way ANOVA method. The results showed that the blood profiles (hematocrit, hemoglobin, erythrocytes, leukocytes, and total plasma protein) with turmeric and meniran extract treatments were still within normal range and not significantly different from the control group (P> 0.05). Based on this research, it can be concluded that the treatment of extract turmeric and meniran or a mixture of both at a dose of 100 mg/kg body weight for 30 days does not affect the blood profile. Keywords: meniran, turmeric, blood profile, chicken   Abstrak Penggunaan sediaan herbal mulai banyak digunakan untuk mendukung kesehatan unggas. Tujuan penelitian ini untuk mendapatkan informasi tentang pengaruh ekstrak kunyit dan meniran serta kombinasi kedua ekstrak terhadap gambaran darah (hematokrit, eritrosit, hemoglobin, leukosit, dan total protein plasma) ayam. Pada penelitian ini digunakan 80 ekor day old chicken (DOC) layer, yang secara acak dibagi menjadi 4 kelompok (A, B, C dan D, masing-masing kelompok 20 ekor). Kelompok A sebagai kontrol, kelompok B sebagai perlakuan yang diberi ekstrak kunyit (dosis 100 mg/kg berat badan), kelompok C diberi ekstrak  meniran (dosis 100 mg/kg BB), dan kelompok D diberi campuran ekstrak meniran dan kunyit (masing-masing dosis 100 mg/kg BB). Pemberian ekstrak kunyit dan meniran dilakukan selama 30 hari. Sampel dikoleksi pada akhir periode penelitian untuk dilakukan pemeriksaan darah. Analisis data secara statistik menggunakan ANOVA one way. Hasil penelitian menunjukkan bahwa pemberian ekstrak kunyit dan meniran tidak berpengaruh signifikan terhadap nilai hematokrit, eritrosit, hemoglobin, leukosit, dan total protein plasma dibanding kontrol (P>0,05). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian ekstrak kunyit dan meniran maupun campuran keduanya dengan dosis 100mg/kg berat badan selama 30 hari tidak berpengaruh terhadap gambaran darah ayam.  Kata kunci: ayam; kunyit; meniran; profil darah

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