Effects of antiserum against alpha-rat atrial natriuretic polypeptide in spontaneously hypertensive rats

1989 ◽  
Vol 257 (4) ◽  
pp. H1104-H1109 ◽  
Author(s):  
A. Sasaki ◽  
O. Kida ◽  
T. Kita ◽  
J. Kato ◽  
S. Nakamura ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Cardiac Output ◽  
Spontaneously Hypertensive Rats ◽  
Physiological Role ◽  
Mean Blood Pressure ◽  
Rabbit Serum ◽  
Normal Rabbit Serum ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Significant Difference

To clarify the physiological role of endogenous atrial natriuretic polypeptide (ANP), we investigated the effects of specific rabbit antiserum against alpha-rat ANP (alpha-rANP) on hemodynamics, diuresis, and natriuresis in spontaneously hypertensive rats (SHR) and Wistar-Kyoto rats (WKY). Intravenous administration of anti-alpha-rANP antiserum caused an obvious rise of mean blood pressure and cardiac output in both SHR and WKY compared with rats administered with normal rabbit serum. Although there was no significant difference in increments of mean blood pressure between SHR and WKY, the increment of cardiac output in SHR was significantly higher than that in WKY. On the other hand, significant reductions in urine output and urinary sodium and potassium excretion lasted for approximately 20 min after administration of the antiserum in both SHR and WKY compared with rats administered with normal rabbit serum. There was no significant difference in these initial maximal decrements between SHR and WKY. These results indicate that endogenous ANP has an important physiological role in the regulation of hemodynamics and water-electrolyte balance in both SHR and WKY. The greater increment of cardiac output in SHR in response to the antiserum suggests that endogenous ANP in SHR may have a stronger cardiosuppressive action that it does in WKY.

Abstract 69: Reduction of Diastolic Dysfunction by Treatment of a Combination of Agonists of Adenosine Receptor in Spontaneously Hypertensive Rats Submitted to Myocardial Infarction

2015 ◽  
Vol 117 (suppl_1) ◽  
Author(s):  
Gisele Zapata-Sudo ◽  
Tais N Frazão ◽  
Jaqueline S da Silva ◽  
Eliezer J Barreiro ◽  
Carlos A Fraga ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Volume Fraction ◽  
Left Ventricular ◽  
Mean Blood Pressure ◽  
Heart Weight ◽  
Hemodynamic Parameters ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive

Introduction: This work investigated the cardioprotective actions of the combination of a positive inotropic agent (LASSBio-294 ) and a potent vasodilator (LASSBio-897) in spontaneously hypertensive rats (SHR) submitted to myocardial infarction (MI). Methods: Twenty four SHR (180-200 g) were randomly divided in sham-operated (SO) and infarcted groups (MI) and each group subdivided in two: treatment with vehicle (DMSO) or with LASSBio-294 + LASSBio-897 (5mg/kg each, p.o.) during 8 weeks. After treatment period, the animals were submitted to echocardiography to determine the anterior wall thickness (AWT), ejection fraction (EF), fractional shortening (FS) and the ratio of early and late transmitral filling velocity (E/A). In addition, the following hemodynamic parameters were evaluated: mean blood pressure (MBP), left ventricular end diastolic pressure (LVEDP), left ventricular end-systolic pressure (LVESP) and LV contractility and relaxation (dp/dt max ). Hypertrophy was measured using the relation between heart weight to body weight (HW/BW). The volume fraction of collagen (%) was determined by measuring the area of H&E stained tissue within a given field. Results: MI induced in SHR promoted a decrease in AWT; EF; FS and E/A from 2.0 ± 0.4 to 1.6 ± 0.9 mm; from 53.1 ± 7.5 to 25.3 ± 6.4 %; from 40.0 ± 0.9 to 25.3 ± 11.0 %; and from 1.4 ± 0.1 to 0.9 ± 0.1, respectively. Treatment with the combination of drugs, increased AWT to 2.5 ± 0.6 mm; EF to 73.2 ± 1.0 %; FS to 43.5 ± 6.6%; and E/A to 1.3 ± 0.1. Increase of LVEDP from 4.6 ± 0.3 to 30.0 ± 3.6 mmHg and duplicated oxygen consumption were observed in MI-SHR. The negative dP/dt was reduced from 6152 ± 1015 to 3957 ± 1225 mm Hg/s. After treatment, all hemodynamic parameters were restored to values similar to SO group. Mean blood pressure which was increased after MI from 168. 2 ± 18.6 to 197.7 ± 10.7 returned to 137.0 ± 19.3 mm Hg after treatment. Increased deposition of colagen from 15.1 ± 3.9 to 24.0 ± 0.9 % induced by MI was prevented with treatment with the combination of drugs (12.9 ± 3.8 %). Conclusion: Oral administration of the combination of LASSBio-294 and LASSBio-897 could be considered promising in preventing cardiac dysfunction in SHR submitted to MI.

Effect of Chronic Clonidine Treatment and its Abrupt Cessation on Mean Blood Pressure of Rats with a Normal or An Elevated Blood Pressure

1979 ◽  
Vol 57 (2) ◽  
pp. 195-201 ◽  
Author(s):  
J. Atkinson ◽  
Nicole Boillat ◽  
Roselyne Pera-Bally ◽  
Lise Peters-Haefeli ◽  
E. J. Kirchertz
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Early Stage ◽  
Mean Blood Pressure ◽  
Average Dose ◽  
Elevated Blood ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Induced Hypertension ◽  
Drinking Fluid

1. Clonidine (6 mg of base/1 of water) was given as drinking fluid to normotensive rats or rats with established or early hypertension. 2. Spontaneous hypertensive rats (6 months old: average dose of clonidine, 0·6 mg 24 h−1 kg−1) showed a sustained fall in blood pressure over 3 weeks. 3. The same clonidine solution given for 6 weeks to two-kidney Goldblatt rats with early-stage hypertension (average dose of clonidine: 1 mg 24 h−1 kg−1) or spontaneously hypertensive rats (clonidine dose: 1 mg) induced a fall in mean blood pressure, but no change in normotensive rats. 4. Replacement of clonidine by water induced hypertension and lability which led to death in hypertensive but not in normotensive rats.

scholarly journals Bioequivalence study of four different trademarks of enalapril maleate in spontaneously hypertensive rats

2008 ◽  
Vol 23 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Nilo César do Vale Baracho ◽  
Guilherme D'Andréa Saba Arruda ◽  
Lidinei José Alves ◽  
Márcio Felipe Salomon Carneiro ◽  
Matheus Teodoro Grilo Siqueira ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Systemic Disease ◽  
Control Group ◽  
High Morbidity ◽  
Hypertensive Rats ◽  
Reference Drug ◽  
Spontaneously Hypertensive ◽  
Enalapril Maleate ◽  
Significant Difference

INTRODUCTION: High blood pressure is a systemic disease which has major clinical and psycho-social repercussions, involves a high morbidity-mortality rate and generates high costs for the health system. Its treatment involves the use of antihypertensive drugs, which are commercialized as trademark, generic or similar drugs. PURPOSE: To verify the antihypertensive effect produced by a similar dose of different trademarks of enalapril maleate in spontaneously hypertensive rats (SHR). METHODS: Fifteen mg/kg of enalapril maleate were administered by gavage in 50 SHR rats and their blood pressure was verified through tail plethysmography every three days in a period of 16 days. RESULTS: The group treated with reference drug has shown a significant reduction on blood pressure levels when compared to the control group. Thus, treatments with enalapril maleate of generic, similar-A and similar-B brands have also shown significant reduction on animals' blood pressure. CONCLUSION: The use of generic drug and similars (A and B) drugs in the same doses and for the same period of time has not shown significant difference regarding the reference drug, which suggests that the brands tested are bioequivalent.

scholarly journals Antihypertensive and Renal Protective Effects of Oryeongsan in Spontaneously Hypertensive Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Kiwan Kang ◽  
Minjeong Jeong ◽  
Hongjun Kim ◽  
Beomjin Lim ◽  
Sangjun Kim ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Treated Group ◽  
Renal Tissue ◽  
Quality Data ◽  
Protective Effects ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Significant Difference ◽  
History Of Use

Oryeongsan (ORS), a traditional medicine used to regulate body fluids, has a long history of use as a diuretic in Korea, China, and Japan. ORS is commonly thought to lower blood pressure, but high-quality data on its effects are sparse. The purpose of this study was to determine the antihypertensive and renal protective effects of ORS in rats with hypertension. Spontaneously hypertensive rats (SHR) were divided into two groups with similar mean baseline systolic blood pressure (SBP) and diastolic blood pressure (DBP). Then, 10 mL/kg of vehicle (distilled water) or 200 mg/kg of ORS extract were administered orally once a day for 3 weeks. SBP and DBP were measured at weeks 1, 2, and 3. At the end of the experiment, blood was collected, and kidneys were removed for histology. By the 2nd and 3rd week after initiation of treatment, the ORS-treated group had significantly lower SBP than control-treated rats (191.3 ± 6.5 vs. 206.3 ± 9.8 mmHg, p  = 0.022 at the 2nd week; 195.8 ± 7.8 vs. 217.0 ± 8.1 mmHg, p  = 0.003 at the 3rd week, respectively). The ORS-treated group trended toward having a lower DBP than control, but there was no significant difference. Blood urea nitrogen (BUN) and serum creatinine (Cr) were not different between the ORS-treated and control groups (BUN: 23.7 ± 1.1 vs. 22.7 ± 2.8 mg/dL, p  = 0.508; Cr: 19.0 ± 2.2 vs. 21.6 ± 2.1 μM, p  = 0.083, respectively). The percentage of renal tissue affected by tubulointerstitial fibrosis was significantly lower in the ORS-treated group (1.68 ± 0.60) compared to controls (3.17 ± 0.96, p  = 0.019). These findings suggest that treatment with ORS reduces SBP and ameliorates renal damage in SHR.

Successful vitrification of stroke-prone spontaneously hypertensive and normal Wistar rat 2-cell embryos

1996 ◽  
Vol 30 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Masahiro Sato ◽  
Kazuo Yokokawa ◽  
Kazuhiro Kasai ◽  
Norihiro Tada
Keyword(s):  
Blood Pressure ◽  
Spontaneously Hypertensive Rats ◽  
Room Temperature ◽  
Wistar Rat ◽  
Hypertensive Rats ◽  
Phenotypic Characteristics ◽  
Spontaneously Hypertensive ◽  
Significant Difference ◽  
One Step ◽  
Wistar Kyoto

Stroke-prone spontaneously hypertensive rats (SHRSPs) have been widely used as models of hypertension and cerebral apoplexy. They were obtained by selective sib-breeding of Wistar Kyoto rats with higher blood pressure than rats of the original Wistar Kyoto strain. For vitrification of SHRSP 2-cell embryos, DPS solution containing 2.75 M dimethylsulfoxide, 2.75 M propylene glycol and 1.0 M sucrose was prepared and diluted in a modified phosphatebuffered saline, PBl, containing 0.3% bovine serum albumin. Embryos were exposed to the resulting solution in one step at room temperature, kept in the solution for 15 s, vitrified in liquid nitrogen, and warmed rapidly. The post-warming survival rate as morphologically assessed was 70% (148/210), which was comparable ( P>0.05) to the rate of 88% (78/89) for the solution control. After vitrification, the embryos were transferred into recipient animals, and 62% (48/78) were normally delivered, comparable ( P>0.05) to the percentage for the solution control (68%, 57/84). These was no significant difference between pups from vitrified embryos and those from unvitrified control embryos in either the growth curve or degree of blood pressure increase. These findings demonstrate the effectiveness of the simple vitrification method we used for cryopreservation of SHRSP and Wistar rat 2-cell embryos, and also demonstrate that vitrification-mediated cryopreservation does not affect the phenotypic characteristics of SHRSPs.

scholarly journals Efficacy and Safety ofGwakhyangjeonggi-SanRetention Enema in Normal Rats and Spontaneously Hypertensive Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-10
Author(s):  
Eunyoung Song ◽  
Euiju Lee ◽  
Yongmin Bu ◽  
Junhee Lee ◽  
Seungwon Shin ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Body Weight ◽  
Treatment Group ◽  
Spontaneously Hypertensive Rats ◽  
Antihypertensive Effect ◽  
Time Effect ◽  
Hypertensive Rats ◽  
Retention Enema ◽  
Spontaneously Hypertensive ◽  
Significant Difference

The purpose of this study is to establish a protocol of retention-enema experiments and evaluate the antihypertensive effect and the safety ofGwakhyangjeonggi-sanretention enema. Normal and spontaneously hypertensive rats (SHRs) were divided into treatment and control groups, respectively. We applied theGwakhyangjeonggi-sanextract by decoction and 0.9% NaCl in each group, estimated the blood pressure and body weight, and performed HPLC analysis. ALT, AST, BUN, and creatinine were examined. The systolic blood pressure within each group in normal rats differed significantly in time effect, and so did the diastolic blood pressure in the treatment group of normal rats. The systolic, diastolic, and mean blood pressure showed significant differences in group effect in the treatment group of the SHRs. The time effect of the body weight in both groups of normal rats differed significantly, so did group × time and time effects in both groups of SHRs. AST, ALT, BUN, and creatinine showed no significant difference between groups. We concluded that theGwakhyangjeonggi-sanretention enema has a hypotensive effect in normal rats within the regular range of blood pressure, but an antihypertensive effect in SHRs. Also, the intervention is safe and does not affect the liver and kidney functions in normal rats.

scholarly journals Antihypertensive Effect Of Magnesium Sulfate (Cormagnesin®) And Its Combination With Furosemide On Conscious Spontaneously Hypertensive Rats

2015 ◽  
Vol 8 (2) ◽  
pp. 112-117
Author(s):  
Katerina D. Simeonova ◽  
Krassimir D. Dimitrov ◽  
Nikolay D. Danchev
Keyword(s):  
Blood Pressure ◽  
Magnesium Sulfate ◽  
Spontaneously Hypertensive Rats ◽  
Antihypertensive Effect ◽  
Hypotensive Effect ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Arterial Blood ◽  
Significant Difference ◽  
Dose Dependent

SummaryThe present study demonstrates the antihypertensive effect of magnesium sulfate (Cormagnesin®) and its combination with Furosemide on conscious spontaneously hypertensive rats (SHR) after intravenous infusion. Experiments were carried out on six groups of conscious male SHR (n=6). Under short anesthesia the rats were chronically instrumented for intravenous (i.v.) drug administration. The arterial blood pressure (AP) was measured by indirect tale method. Cormagnesin®was applied by i.v. infusion in doses of 5, 20 and 40 mg/kg; and furosemide (10 mg/kg) was applied intraperitoneally. Experimental results showed significant decrease of AP after i.v. infusion of 20 mg/kg Cormagnesin®as well as after application of the Cormagnesin®and furosemide combination. The hypotensive effects of 40 mg/kg Cormagnesin®and of furosemide were not significant. There was no significant difference between the antihypertensive effects of Cormagnesin®and its combination with furosemide but the combination showed much better hypotensive effect than Furosemide (p<0.05). Our study demonstrated the antihypertensive effect of magnesium sulfate on conscious SHR after i.v. application. Our results suggest that the antihypertensive effect of magnesium sulfate in the doses applied is not dose-dependent. Magnesium sulfate potentiates the antihypertensive effect of furosemide in SHR.

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