Mitochondrial lipid peroxidation and superoxide dismutase in rat hypertensive target organs

1995 ◽  
Vol 268 (4) ◽  
pp. H1418-H1421 ◽  
Author(s):  
T. Ohtsuki ◽  
M. Matsumoto ◽  
K. Suzuki ◽  
N. Taniguchi ◽  
T. Kamada
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Reactive Substance ◽  
Target Organs ◽  
Significant Difference ◽  
The Brain

Mitochondrial respiratory chains leak a large amount of superoxide anion radicals, which chain react with membrane phospholipid to develop lipid peroxidation. Manganese superoxide dismutase (MnSOD) is then inducible and catalyzes superoxide detoxification within mitochondria. We examined mitochondrial thiobarbituric acid-reactive substance, an end product of lipid peroxidation, and MnSOD concentration in hypertensive target organs of spontaneously hypertensive and deoxycorticosterone acetate salts-induced hypertensive rats. Normotensive rats showed significant increases in thiobarbituric acid-reactive substance and MnSOD in the brain as they matured. Mature spontaneously hypertensive and induced hypertensive rats showed a marked elevation of lipid peroxidation but no increase in superoxide dismutase in the brain. The heart and kidney presented no significant difference of lipid peroxidation and superoxide dismutase among strains, ages, and treatments. Abnormal mitochondrial metabolism of oxygen radicals was observed selectively in the brain during hypertension and may contribute to mitochondrial injury and lead to neuronal degeneration or susceptibility to brain ischemia in mature hypertensive rats.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

scholarly journals Antioxidant Action of Solid Preparation of Xingnaojing in SHRSP

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Yang Liu ◽  
Naomi Yasui ◽  
Aya Kishimoto ◽  
Jian-ning Sun ◽  
Katsumi Ikeda
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Antioxidant Effect ◽  
Antioxidant Action ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Total Antioxidant

We investigated the antioxidant action of a solid preparation of Xingnaojing (XNJ) and ascorbic acid (AA) in stroke-prone spontaneously hypertensive rats (SHRSP). The total antioxidant states in the plasma, systolic blood pressure, and heart rate were measured every 2 weeks, and lipid peroxidation, expressed as thiobarbituric acid-reactive substances in plasma, was measured in the 6th week. The results showed that AA and XNJ significantly increased the total antioxidant status in plasma and reduced malondialdehyde in the plasma. These data suggest that during 6-week administration, XNJ has antioxidant action on SHRSP, which may relate to its generalized inhibition of lipid peroxidation and promotion of the total antioxidant state. These results demonstrated that orally treated XNJ has an antioxidant effect on SHRSP plasma.

Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia

1997 ◽  
Vol 272 (2) ◽  
pp. L294-L300 ◽  
Author(s):  
A. B. Al-Mehdi ◽  
H. Shuman ◽  
A. B. Fisher
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Reactive Oxygen ◽  
Thiobarbituric Acid ◽  
Conjugated Dienes ◽  
Thiobarbituric Acid Reactive Substance ◽  
Oxygen Species ◽  
Reactive Substance ◽  
Rat Lungs

Surface fluorometry with 40 microM hydroethidine (HE) as a probe was used to detect oxidant generation in isolated, ventilated rat lungs during lung ischemia. Ethidium fluorescence due to HE oxidation was continuously monitored with 470 nm excitation and 610 nm emission. Fluorescence increased with ischemia in O2-ventilated lungs [0.98 +/- 0.08 arbitrary fluorescence units (AFU)/min vs. 0.58 +/- 0.07 with control perfusion]. HE oxidation during ischemia was prevented by N2 ventilation but was unaltered by preperfusion with superoxide dismutase. Ethidium fluorescence in homogenate prepared from lungs subjected to 1 h of nonhypoxic ischemia was increased (16.8 +/- 1.5 vs. 9.8 +/- 0.4 AFU/mg protein in control) but was unchanged in lungs that had been N2 ventilated. Microfluorographs of HE perfused and fixed lung sections demonstrated marked generalized increases in ethidium fluorescence with ischemia compared with control perfusion. Ischemia resulted in significant increases in tissue thiobarbituric acid reactive substance (176 +/- 13 vs. 44 +/- 3 pmol/mg protein for control) and in lung conjugated dienes (0.90 +/- 0.07 vs. 0.48 +/- 0.06 U/mg protein for control), indicating peroxidation of lung lipids. These results indicate that lung ischemia leads to intracellular oxidant generation that can be continuously monitored by surface fluorometry.

scholarly journals IN VIVO ANTIOXIDANT ACTIVITY OF LIMNOPHILA HETEROPHYLLA AND MICHELIA CHAMPACA

2017 ◽  
Vol 9 (12) ◽  
pp. 241 ◽  
Author(s):  
Raja S, ◽  
Ravindranadh K.
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Reactive Substance ◽  
Reducing Ability ◽  
Liver And Kidney ◽  
Michelia Champaca ◽  
Different Doses

Objective: The present study was aimed at investigating the in-vivo antioxidant activity of the methanol extracts of Limnophila heterophylla and Michelia champaca leaves.Methods: Methanol extract of both plants were administered to rats separately at three different doses of 125, 250 and 500 mg/kg for 21 d to evaluate oxidative stress parameters such as ferric reducing ability of plasma (FRAP), thiobarbituric acid reactive substance (TBARS) and reduced glutathione (GSH) and to evaluate antioxidant enzyme levels of catalase (CAT) and superoxide dismutase (SOD).Results: The methanol extracts of both the plants significantly (p<0.05) elevated the ferric reducing ability of plasma (FRAP) on days 7, 14 and 21 of treatment. Significant (p<0.05) decrease of thiobarbituric acid reactive substance (TBARS) levels along with an increase in the superoxide dismutase (SOD) enzyme level in the liver and kidney at three different doses both the plants was observed. Treatment at a dose of 500 mg/kg b. w of both plants caused a significant increase only in the level of CAT in the liver and kidney. However, there was no significant effect of a thiobarbituric acid reactive substance (TBARS), superoxide dismutase (SOD) and catalase (CAT) in the heart and reduced glutathione (GSH) level in liver, heart and kidney at three different doses both the plants.Conclusion: These outcomes recommend that the leaves of Limnophila heterophylla and Michelia champaca have a potent antioxidant activity which may be responsible for some of its reported pharmacological actions. 

scholarly journals Inhibitory Response ofRaphanus sativuson Lipid Peroxidation in Albino Rats

2008 ◽  
Vol 5 (1) ◽  
pp. 55-59 ◽  
Author(s):  
P. Chaturvedi
Keyword(s):  
Lipid Peroxidation ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Cumene Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Experimental Control ◽  
Reactive Substance

In the present study, inhibitory effect of the methanol extract ofRaphanus sativusroot on lipid peroxidation has been carried out in normal rats. Graded doses of methanol extract of root of the plant (40, 80 and 120 mg kg−1body weight) were administered orally for 15 days to experimental treated rats. Distilled water was administered to experimental control rats. At the end of experiment, rats were killed by decapitation after ether anesthesia. Blood and liver were collected to measure thiobarbituric acid reactive substance, reduced glutathione and activity of catalase. Results indicated that the extract ofR. sativusroot reduced the levels of thiobarbituric acid reactive substance significantly in all experimental treated groups (P < 0.05) as compared to the experimental control group. It also increased the levels of reduced glutathione and increased the activity of catalase.In vitroexperiments with the liver of experimental control and experimental treated rats were also carried out against cumene hydroperoxide induced lipid peroxidation. The extract inhibitedin vitrocumene hydroperoxide induced lipid peroxidation.R. sativusinhibits lipid peroxidationin vivoandin vitro. It provides protection by strengthening the antioxidants like glutathione and catalase. Inclusion of this plant in every day diet would be beneficial.

scholarly journals Evaluation of theIn VitroandIn VivoAntioxidant Potentials ofAframomum meleguetaMethanolic Seed Extract

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Samuel Okwudili Onoja ◽  
Yusuf Ndukaku Omeh ◽  
Maxwell Ikechukwu Ezeja ◽  
Martins Ndubuisi Chukwu
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Seed Extract ◽  
Assay Method ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Reactive Substance ◽  
Photometric Assay ◽  
Antioxidant Effects

Aframomum meleguetaSchum (Zingiberaceae) is a perennial herb widely cultivated for its valuable seeds in the tropical region of Africa. The present study evaluated the antioxidant effects of methanolic seed extract ofA. melegueta. The antioxidant effects were evaluated usingin vitro, 2, 2-diphenylpicrylhydrazine photometric assay andin vivoserum catalase, superoxide dismutase and thiobarbituric acid reactive substance assay method. The extract (25–400 μg/mL concentration) produced concentration dependent increase in antioxidant activity in 2, 2-diphenylpicrylhydrazine photometric assay. The extract (400 mg/kg) showed a significant (P<0.05) increase in serum catalase and superoxide dismutase activity when compared with the control group. The extract (400 mg/kg) showed a significant (P<0.05) decrease in the serum level of thiobarbituric acid reactive substance when compared with the control group. These findings suggest that the seed ofA. meleguetahas potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

Analysis of Oxidative Stress Indicators in Polish Patients With Prostate Cancer

2021 ◽  
Author(s):  
Joanna Maria Drozdz-Afelt ◽  
Beata Barbara Koim-Puchowska ◽  
Piotr Kaminski
Keyword(s):  
Prostate Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Detoxification Enzymes ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Significant Difference

Abstract Aims: The aim of the study was to analyze the activity of antioxidant enzymes (glutathione S-transferase, catalase, superoxide dismutase) in order to determine the role of detoxification mechanisms in prostate cancer. The concentration of malondialdehyde, which is an indicator of lipid peroxidation in cancer patients, was also tested.Methods: The activities of superoxide dismutase (SOD), catalase CAT and glutathione S-transferase (GST ) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method.Results: Superoxide dismutase was the only enzyme among antioxidant and detoxification enzymes for which a statistically significant difference in activity was found between the studied groups [1.4 U * ml-1 in patients vs. 1.6 U * ml-1 in control]. No statistically significant differences were found for two other biomarkers of antioxidant activity (GST, CAT). There were also no statistically significant differences in the concentration of MDA between the group of men with prostate cancer and the control group.Conclusion: The lower SOD activity in men with prostate cancer may be due to a deficiency in their antioxidant defense system.

scholarly journals Neuroprotective and Antiamnesic Effects ofMitragyna inermisWilld (Rubiaceae) on Scopolamine-Induced Memory Impairment in Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
David Bougolla Pahaye ◽  
Elisabeth Ngo Bum ◽  
Germain Sotoing Taïwé ◽  
Gwladys Temkou Ngoupaye ◽  
Neteydji Sidiki ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Object Recognition ◽  
Elevated Plus Maze ◽  
Recognition Task ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Activity Levels ◽  
Reactive Substance ◽  
Object Recognition Task ◽  
Plus Maze

Aim. To assess memory improvement and neuroprotective and antioxidant effects ofMitragyna inermis(M. inermis) leaf decoction on the central nervous system.Methodology. Leaf decoction ofM. inermiswas tested on learning and memory in normal and scopolamine-induced cognitive impairment in mice using memory behavioral tests such as the Morris water maze, object recognition task, and elevated plus maze. Oxidative stress enzymes—catalase, superoxide dismutase, and the thiobarbituric acid reactive substance, a product of lipid peroxidation—were quantified. In each test, mice 18 to 25 g were divided into groups of 5.Results. The extract reversed the effects of scopolamine in mice. The extract significantly increased discrimination index in the object recognition task test and inflexion ratio in the elevated plus maze test. The times spent in target quadrant in MWM increased while the transfer latency decreased in mice treated byM. inermisat the dose of 196.5 mg/kg. The activity levels of superoxide dismutase and catalase were significantly increased, whereas the thiobarbituric acid reactive substance was significantly decreased after 8 consecutive days of treatment withM. inermisat the dose of 393 mg/kg.Conclusion. These results suggest thatM. inermisleaf extract possess potential antiamnesic effects.

scholarly journals Thiobarbituric acid reactive substances as marker of oxidative stress in pregnancies with pre-eclampsia

2011 ◽  
Vol 64 (7-8) ◽  
pp. 377-380 ◽  
Author(s):  
Aleksandra Novakov-Mikic ◽  
Snezana Brkic ◽  
Daniela Maric ◽  
Bojan Sekulic ◽  
Aleksandar Cetkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Screening Method ◽  
Systolic Arterial Pressure ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reactive Substance ◽  
To Come ◽  
Tbars Assay

Pre-eclampsia is characterized by increased lipid peroxidation and diminished antioxidant capacity. The aim of the study was to establish concentration of thiobarbituric acid reactive substances as a marker of lipid peroxidation in normal pregnancies and in pregnancies complicated with pre-eclampsia, and to estimate the possibility of using thiobarbituric acid reactive substances as a screening method for development of pre-eclampsia. The study was conducted at the Department of Obstetrics and Gynaecology, Clinical Centre of Vojvodina. The study included 57 singleton pregnancies, gestation >24 weeks, of which 29 were healthy pregnancies and 28 were with pre-eclampsia, defined as systolic arterial pressure of >90 mmHg, diastolic of >145 mmHg, and 24h proteinuria of >300mg. Thiobarbituric acid reactive substances concentrations evaluated by malondialdehyde equivalent standards (OxiSelect? TBARS Assay Kit (malondialdehyde Quantitation), Cell Biolabs? OxiSelect?) showed that oxidative stress was more evident in the group with pre-eclampsia, though not statistically significant (p= 0.107). There was no correlation of thiobarbituric acid reactive substance levels with gestation in either group. The differences between the level of thiobarbituric acid reactive substance concentrations in pre-eclampsia and healthy pregnancies indicate the possibility of using thiobarbituric acid reactive substances as a screening tool for the development of pre-eclampsia. Further studies with larger numbers of patients are needed in order to come to final conclusions.

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