Tactoid formation in deer hemoglobin
Sickle cell preparations were made on eight Virginia white-tail deer ( Odocoileus virginianus) killed during the 1958–59 hunting season. All preparations showed sickling. Paper electrophoresis of carboxyhemoglobin from these animals revealed identical mobilities and a single hemoglobin component present at pH 8.6. Alkali denaturation of the deer carboxyhemoglobin solution showed that it was markedly resistant to alkali denaturation and that the kinetics of the denaturation were different from that of human cord hemoglobin. Deer carboxyhemoglobin migrated 0.6 x 10–5 cm2/volt/sec.–1 faster than human carboxyhemoglobin A in moving boundary electrophoresis in veronal buffer at pH 8.2. Tactoids were demonstrated in free hemoglobin solution with phase microscopy after concentration of the solution. These particles are very similar morphologically to these prepared from hemoglobin solution containing hemoglobin S.