Normalization of force generated by canine airway smooth muscles

1991 ◽  
Vol 260 (6) ◽  
pp. L522-L529 ◽  
Author(s):  
H. Jiang ◽  
A. J. Halayko ◽  
K. Rao ◽  
P. Cunningham ◽  
N. L. Stephens
Keyword(s):  
Smooth Muscle ◽  
Cross Section ◽  
Cross Sections ◽  
Smooth Muscles ◽  
Muscle Cells ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Muscle Stress ◽  
Total Tissue

A variety of normalizations have been employed to compare maximal isometric force (Po) produced by smooth muscles at different locations and stages of maturation. Because these procedures have not always been based on rigorous principles, confusion has resulted. To obtain a less ambiguous index of force production, we measured in vitro Po from mongrel canine tracheal (TSM) and bronchial (BSM) smooth muscle with an electromagnetic lever and normalized it to force per unit cross-sectional area of whole tissue (tissue stress), to force per unit cross-sectional area of muscle in the cross section of total tissue (muscle stress), and to force per fractional unit of myosin in the tissue cross section (myosin stress). Proportion of myosin in cross-sectional area of tissue was deduced from data obtained by sodium dodecyl sulfate gel electrophoresis of crude muscle extracts. For TSM, tissue stress was 1.499 X 10(5) N/m2 +/- 0.1 (SE), whereas it was only 0.351 X 10(5) N/m2 +/- 0.05 (SE) for BSM, representing a 4.27-fold difference (P less than 0.01). There was a 1.60-fold difference (P less than 0.05) in muscle stress, which was correlated to the morphometric finding that 79 +/- 1.4% (SE) of the tracheal strip cross section was muscle, whereas only 30 +/- 1.0% (SE) of bronchial tissue was occupied by muscle. Average myosin content was the same in smooth muscle cells of TSM and BSM, indicating that total amount of myosin in tissue cross sections was essentially a function of proportional area of muscle cells in total tissue cross sections.(ABSTRACT TRUNCATED AT 250 WORDS)

Normalization of contractile parameters in canine airway smooth muscle: morphological and biochemical

1992 ◽  
Vol 70 (4) ◽  
pp. 635-644 ◽  
Author(s):  
N. L. Stephens ◽  
A. Halayko ◽  
H. Jiang
Keyword(s):  
Smooth Muscle ◽  
Muscle Cell ◽  
Striated Muscle ◽  
Isometric Force ◽  
Smooth Muscles ◽  
Cross Sectional Area ◽  
Unit Weight ◽  
Sectional Area ◽  
Cross Sectional ◽  
Cross Bridges

Asthma research has recently highlighted the importance of correctly normalizing force development for purposes of comparing stiffness properties of smooth muscles between different airways, between airways at different stages of maturity, and between airways from different animal species. This problem does not exist in striated muscle where the entire tissue consists almost entirely of muscle and where cross bridges cycle at the same rate throughout a contraction when load correlation is made. In the bronchus, cross-sectional area of true muscle may constitute only 20–30% of the total tissue cross section, and load-independent cycling rate varies fourfold during the course of a contraction because of the occurrence of normally cycling and latch bridges. These features are responsible for the difficulty in force normalization in smooth muscle. Our studies indicate that normalization with respect to true muscle cell cross-sectional area (derived by quantitative morphometry of appropriate tissue transverse sections) is the most valid. This is only so, however, when it has been proved that the actomyosin content per unit weight of the different muscle tissues is the same.Key words: isometric force, force normalization, muscle cell stress, actomyosin stress.

Comparison of the Cross Sectional Area, the Loss in Volume and the Mechanical Properties of LAE442 and MgCa0.8 as Resorbable Magnesium Alloy Implants after 12 Months Implantation Duration

2010 ◽  
Vol 638-642 ◽  
pp. 675-680 ◽  
Author(s):  
Martina Thomann ◽  
Nina von der Höh ◽  
Dirk Bormann ◽  
Dina Rittershaus ◽  
C. Krause ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cross Sections ◽  
Degradation Process ◽  
Cross Sectional Area ◽  
Bending Test ◽  
Sectional Area ◽  
Cross Sectional ◽  
Initial Strength ◽  
Three Point Bending ◽  
The Cross

Current research focuses on magnesium based alloys in the course of searching a resorbable osteosynthetic material which provides sufficient mechanical properties besides a good biocompatibility. Previous studies reported on a favorable biocompatibility of the alloys LAE442 and MgCa0.8. The present study compared the degradation process of cylindrical LAE442 and MgCa0.8 implants after 12 months implantation duration. Therefore, 10 extruded implants (2.5 x 25 mm, cross sectional area 4.9 mm²) of both alloys were implanted into the medullary cavity of both tibiae of rabbits for 12 months. After euthanization, the right bone-implant-compound was scanned in a µ-computed tomograph (µCT80, ScancoMedical) and nine uniformly distributed cross-sections of each implant were used to determine the residual implants´ cross sectional area (Software AxioVisionRelease 4.5, Zeiss). Left implants were taken out of the bone carefully. After weighing, a three-point bending test was carried out. LAE442 implants degraded obviously slower and more homogeneously than MgCa0.8. The mean residual cross sectional area of LAE442 implants was 4.7 ± 0.07 mm². MgCa0.8 showed an area of only 2.18 ± 1.03 mm². In contrast, the loss in volume of LAE442 pins was more obvious. They lost 64 % of their initial weight. The volume of MgCa0.8 reduced clearly to 54.4 % which corresponds to the cross sectional area results. Three point bending tests revealed that LAE442 showed a loss in strength of 71.2 % while MgCa0.8 lost 85.6 % of its initial strength. All results indicated that LAE442 implants degraded slowly, probably due to the formation of a very obvious degradation layer. Degradation of MgCa0.8 implants was far advanced.

scholarly journals The accuracy of land form presentation in forest road Cross sections

2002 ◽  
pp. 91-99
Author(s):  
Bogdan Stefanovic ◽  
Velimir Aleksic
Keyword(s):  
Cross Sections ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Land Form ◽  
Volume Calculation ◽  
Forest Road ◽  
Earth Material ◽  
Legal Provisions ◽  
Natural Land

Accurate presentation of natural land forms directly affects the accuracy of earth volume calculation, the component of technical documentation of the forest road master project. Consistent to legal provisions, forest road master project is characterised by high accuracy of the planned scope of works compared to the realised scope of works, which should amount to ?3%. The volume of earth material is calculated based on the road cross-sectional area and the distance between the cross sections. The accuracy of the area of cross sections depends on the accuracy of land form presentation Accurate presentation of natural land forms depends on a great number of factors, the most significant of which are the methods of surveying and acquisition of data necessary for land form presentation, preciseness of equipment and instruments used in surveying and acquisition of data, and the method of land form presentation. The accuracy of earth material volume calculation depends on the method of computation, method of presentation of natural and artificial cross sections, and the phase of design. The presentation of natural land form in the width occupied by roadway cross section depends on the validity of the obtained station point elevation (Ht) and the elevations of detailed points (Hi). A relevant factor of the accuracy of cross sectional area, compared to its other elements, is the accuracy of the depth of cut or the height of fill (h) which should agree with the required accuracy, i.e. the adopted tolerance of the earthwork bill of quantities deviation up to 3%.

Relevance of classification by size to topographical differences in bronchial smooth muscle response

1993 ◽  
Vol 75 (5) ◽  
pp. 2013-2021 ◽  
Author(s):  
P. Chitano ◽  
S. B. Sigurdsson ◽  
A. J. Halayko ◽  
N. L. Stephens
Keyword(s):  
Smooth Muscle ◽  
Electrical Field Stimulation ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Muscle Response ◽  
Bronchial Responsiveness ◽  
Sixth Generation ◽  
Significant Difference ◽  
And Control

To investigate heterogeneity of airway smooth muscle response, we studied strips of large and small branches from third- to sixth-generation bronchi obtained from ragweed antigen-sensitized and control dogs. The response to electrical field stimulation and carbamylcholine chloride was greater in strips from larger branches of the same generation when expressed as "tissue stress" (force per unit cross-sectional area of the whole tissue), whereas no difference emerged with use of the more appropriate "smooth muscle stress" (force per unit cross-sectional area of the muscle tissue). The response to histamine was significantly higher in small branches than in large ones, and histamine sensitivity [mean effective concentration (EC50)] was 7.79 x 10(-6) [geometric standard error of the mean (GSEM) 1.20] and 1.49 x 10(-5) M (GSEM 1.14), respectively (P < 0.01). Strips from control and sensitized animals at each site and strips from different generations did not show any significant difference. When we clustered our preparations according to dimensions, the response to histamine was significantly higher in small bronchi than in large ones and histamine EC50 was 8.95 x 10(-6) (GSEM 1.17) and 1.57 x 10(-5) M (GSEM 1.18), respectively (P < 0.05). We conclude that evaluation of muscle response in different tissues requires appropriate normalization. Furthermore, classification into generations is inadequate to study bronchial responsiveness, inasmuch as major differences originate from airway size.

Genetic Effects on Skeletal Mechanosensitivity in Mice

2002 ◽  
Author(s):  
Charles H. Turner ◽  
Alexander G. Robling
Keyword(s):  
Mechanical Loading ◽  
Cross Sections ◽  
Inbred Mouse ◽  
Cross Sectional Area ◽  
Mouse Strains ◽  
Sectional Area ◽  
Mineral Density ◽  
Bone Apposition ◽  
Cross Sectional ◽  
Total Cross Sectional Area

The accumulation of bone mass during growth can be enhanced by environmental factors such as mechanical loading (exercise) or calcium intake, but 60–70% of the variance in adult bone mineral density (BMD) is explained by heredity. Consequently, understanding the signaling pathways targeted by the genes governing bone accumulation holds perhaps the greatest potential in reducing fracture incidence later in life. Rodent models are particularly useful for studying the genetics of skeletal traits. Of the available inbred mouse strains, three in particular have been studied extensively in skeletal genetics: C57BL/6, DBA/2, and C3H/He. The C57BL/6 strain is characterized by low BMD and large total cross-sectional area (CSA) in the midshaft femur; the C3H/He strain exhibits very high femoral BMD and a smaller femoral CSA than the C57BL/6 mice; and DBA/2 mice have moderately high femoral BMD and a very small midshaft femur CSA. Mechanical loading of the skeleton during growth can substantially enhance periosteal bone apposition, and ultimately produce a diaphyseal cross section with enlarged area. Therefore we hypothesized that the mouse strain with greater femoral cross-sectional area (C57BL/6) might have a genetic predisposition for greater mechanosensitivity than mice with smaller cross sections (C3H/He and DBA/2).

Absence of a growth hormone effect on rat soleus atrophy during a 4-day spaceflight

1993 ◽  
Vol 74 (2) ◽  
pp. 527-531 ◽  
Author(s):  
B. Jiang ◽  
R. R. Roy ◽  
C. Navarro ◽  
V. R. Edgerton
Keyword(s):  
Growth Hormone ◽  
Succinate Dehydrogenase ◽  
Cross Sections ◽  
Fiber Type ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Fast Myosin ◽  
Hormone Effect ◽  
Fast Myosin Heavy Chain

The objectives of the present study were to determine the size and enzyme properties of soleus fibers of rats subjected to a 4-day spaceflight (National Aeronautics and Space Administration, STS-41) and the effects of exogenous growth hormone (GH) on the atrophic response of the muscle. Four groups of rats were studied: 1) control (Con), 2) Con plus GH treated (Con + GH), 3) flight (Fl), and 4) F1 plus GH treated (Fl + GH). Cross-sectional area and the activities of succinate dehydrogenase and myofibrillar adenosinetriphosphatase (ATPase) were determined in fibers identified in frozen serial cross sections. Fibers were categorized immunohistochemically as slow, fast, or slow-fast on the basis of their reaction with slow and fast myosin heavy-chain (MHC) monoclonal antibodies. Fibers also were categorized as light or dark on the basis of their staining for ATPase at pH 8.6. After the 4-day flight, mean body weight was significantly decreased compared with control. The absolute and relative (muscle wt/body wt) soleus weights were significantly smaller in the Fl and Fl + GH rats compared with their respective ground-based controls. In both flight groups, the cross-sectional area of the light ATPase fibers was significantly smaller (approximately 30%) than control. Three of 11 flight rats had a higher proportion of fibers expressing both slow and fast MHCs than expected on the basis of the fiber type distribution in the 11 control rats. Mean fiber succinate dehydrogenase and ATPase activities were similar among the four groups.(ABSTRACT TRUNCATED AT 250 WORDS)

Conservation of bronchiolar wall area during constriction and dilation of human airways

1997 ◽  
Vol 82 (3) ◽  
pp. 954-958 ◽  
Author(s):  
R. W. Mitchell ◽  
E. Rühlmann ◽  
H. Magnussen ◽  
N. M. Muñoz ◽  
A. R. Leff ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Contraction ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Wall Area ◽  
Total Cross Sectional Area ◽  
Human Airways ◽  
Contraction And Relaxation

Mitchell, R. W., E. Rühlmann, H. Magnussen, N. M. Muñoz, A. R. Leff, and K. F. Rabe. Conservation of bronchiolar wall area during constriction and dilation of human airways. J. Appl. Physiol. 82(3): 954–958, 1997.—We assessed the effect of smooth muscle contraction and relaxation on airway lumen subtended by the internal perimeter ( A i) and total cross-sectional area ( A o) of human bronchial explants in the absence of the potential lung tethering forces of alveolar tissue to test the hypothesis that bronchoconstriction results in a comparable change of A iand A o. Luminal area (i.e., A i) and A owere measured by using computerized videomicrometry, and bronchial wall area was calculated accordingly. Images on videotape were captured; areas were outlined, and data were expressed as internal pixel number by using imaging software. Bronchial rings were dissected in 1.0- to 1.5-mm sections from macroscopically unaffected areas of lungs from patients undergoing resection for carcinoma, placed in microplate wells containing buffered saline, and allowed to equilibrate for 1 h. Baseline, A o[5.21 ± 0.354 (SE) mm2], and A i(0.604 ± 0.057 mm2) were measured before contraction of the airway smooth muscle (ASM) with carbachol. Mean A inarrowed by 0.257 ± 0.052 mm2in response to 10 μM carbachol ( P = 0.001 vs. baseline). Similarly, A onarrowed by 0.272 ± 0.110 mm2in response to carbachol ( P = 0.038 vs. baseline; P = 0.849 vs. change in A i). Similar parallel changes in cross-sectional area for A iand A owere observed for relaxation of ASM from inherent tone of other bronchial rings in response to 10 μM isoproterenol. We demonstrate a unique characteristic of human ASM; i.e., both luminal and total cross-sectional area of human airways change similarly on contraction and relaxation in vitro, resulting in a conservation of bronchiolar wall area with bronchoconstriction and dilation.

Modelling the Acoustical and Airflow Performance of Simple Lined Ventilation Apertures

2005 ◽  
Vol 12 (4) ◽  
pp. 277-292 ◽  
Author(s):  
D J Oldham ◽  
Jian Kang ◽  
M W Brocklesby
Keyword(s):  
Aspect Ratio ◽  
Cross Section ◽  
High Aspect Ratio ◽  
Natural Ventilation ◽  
Ventilation System ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Cross Sectional ◽  
Ventilation Performance ◽  
Acoustic Treatment

The pressure differences that can be used to drive a natural ventilation system are very small and thus large apertures are required to allow sufficient air to enter and leave a building to ensure good air quality or thermal comfort. Large apertures are potential acoustic weak points on a façade and may require some form of acoustic treatment such as absorbent linings, in which case the ventilator is similar to a short section of lined duct. In ducts, the performance of absorbent linings increases with the length of lining and the ratio of the length of lined perimeter to the cross sectional area of the duct. Thus, for a duct of a given cross sectional area, a lining is more effective for a duct with a high aspect ratio than for a duct with a square cross section. However, the high aspect ratio cross section will result in greater flow resistance and impede the airflow performance. In this paper numerical methods are employed to investigate the effect of different configurations of a lined aperture on the acoustical and ventilation performance of the aperture in order to establish the optimum configurations.

scholarly journals The assessment sectional area of stem wood and stocks in experimental trees reclamation Western Donbass plot № 1

2016 ◽  
Vol 45 ◽  
pp. 76-81
Author(s):  
V. M. Zverkovsky ◽  
O. S. Zubkova
Keyword(s):  
Cross Sections ◽  
Linear Growth ◽  
Cross Sectional Area ◽  
Forest Plantations ◽  
Sectional Area ◽  
Cross Sectional ◽  
Stem Wood ◽  
Diameter At Breast Height ◽  
Breast Height

The linear growth of forest plantations of Western Donbass’ recultivating plot №1 were studied. The established cross-sectional the area of trunks and timber reserves experimental trees.Characterized by parameters which are the volume of wood: its height, diameter at breast height and shape of the forming barrel. In diameter at breast height cross sectional area is determined and then the barrel volume is calculated. Cross sections of tree trunks are shaped like a circle or an ellipse. Knowing the volume of logs we calculated reserves of wood for trees experimental plots.The largest reserves of timber and cross-sectional area characterized planting U. pumila – 15,367 m3 and 1,9583 m2, A. platanoides – 13,328 m3 and 2,67 m2, Q. robur – 10,120 m3 and 1,452 m2, J. virginiana – 8,748 m3 and 2,106 m2. The least plantation stocks of wood characterized E. angustifolia – 1,3699 m3 and 0,3693 m2, R. pseudoacacia – 2,9478 m3 and 0,8350 m2, P. rallasiana – 3,1626 m3 and 0,3279 m2.

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