Compensation for an increase in body fat caused by donor transplants into mice

2004 ◽  
Vol 286 (6) ◽  
pp. R1149-R1155 ◽  
Author(s):  
Cherie Rooks ◽  
TaNeisha Bennet ◽  
Timothy J. Bartness ◽  
Ruth B. S. Harris
Keyword(s):  
Body Fat ◽  
Statistical Significance ◽  
Subcutaneous Fat ◽  
Cell Number ◽  
Adipocyte Size ◽  
Fat Depots ◽  
Siberian Hamsters ◽  
Epididymal Fat ◽  
Fat Stores ◽  
Total Body

Rodents tend to compensate for experimental obesity in which both adipocyte size and number are increased. In contrast, it was recently reported that Siberian hamsters do not compensate for dorsal subcutaneous transplants of fat, which increase body fat without changing the size of adipocytes. In the first experiment described here we tested whether mice changed the size of their endogenous fat stores 2 or 5 wk after donor fat was added as subcutaneous transplants. Each epididymal fat pad from donor mice was cut in half and placed ventrally in recipient mice, increasing body fat by ∼10%. After 2 wk, there was no effect of the transplants on the size of endogenous fat depots or the size of adipocytes in epididymal fat depots. There was a substantial decrease in mass and adipocyte size in transplanted fat. Five weeks after surgery the endogenous epididymal and retroperitoneal fat depots of recipient mice were significantly decreased, serum leptin was reduced, and the size of adipocytes in endogenous epididymal fat was significantly reduced, although cell number had not changed. The size of transplanted cells was the same as at 2 wk. In a second experiment, epididymal fat was placed as either dorsal or ventral subcutaneous fat transplants. Five weeks after surgery the endogenous fat depots were decreased in all recipient mice but none of the differences reached statistical significance. These results suggest that mice have mechanisms to maintain total body fat mass that respond to an increase in the number of fat cells present.

The partition of body fat in British Friesian and Jersey steers

1982 ◽  
Vol 35 (2) ◽  
pp. 253-262 ◽  
Author(s):  
B. W. Butler-Hogg ◽  
J. D. Wood
Keyword(s):  
Body Fat ◽  
Milk Fat ◽  
Subcutaneous Fat ◽  
Age Groups ◽  
Live Weight ◽  
The Body ◽  
Fat Depots ◽  
Breed Difference ◽  
Intermuscular Fat ◽  
Total Body

ABSTRACTNinety-two British Friesians and 62 Jersey castrated male cattle were slaughtered serially in five age groups at 13, 89, 170, 339 and 507 days, and dissected fully into lean, bone, intermuscular fat, subcutaneous fat, perirenal-retroperitoneal fat (kidney knob and channel fat), omental fat and mesenteric fat. The aim was to investigate the partition of body fat in these dairy breeds and the role of the partition of fat in determining carcass value.Relative to live weight, Friesians had more lean, subcutaneous fat and carcass fat (subcutaneous and intermuscular) at most ages, and Jerseys had more kidney knob and channel fat, and intra-abdominal fat. Friesians had a higher killing-out proportion and lean:bone ratio, and thicker subcutaneous fat.The order of increasing relative growth of fat depots with total body fat as the independent variable was, for Friesians: intermuscular < mesenteric < kidney knob and channel fat < subcutaneous < omental. In Jerseys the order was: intermuscular < mesenteric < subcutaneous < kidney knob and channel fat < omental. There were only small breed differences in the distribution of subcutaneous fat between eight regions. t I is suggested that, between breeds, there is a physiological link between the capacity for milk-fat production and the partition of fat within the body, with relatively high milk-fat producers depositing proportionately more fat intra-abdominally.Since the timing of slaughter is often determined by level of external finish in beef production, the breed difference in the partition of fat, which caused Jerseys to have a higher proportion of kidney knob and channel fat, and intermuscular fat, at the same proportion of subcutaneous fat, would reduce carcass value in Jerseys compared with Friesians.

Body fat regulation after partial lipectomy in Siberian hamsters is photoperiod dependent and fat pad specific

1994 ◽  
Vol 266 (3) ◽  
pp. R870-R878 ◽  
Author(s):  
M. M. Mauer ◽  
T. J. Bartness
Keyword(s):  
Body Fat ◽  
Fat Pad ◽  
Compensatory Response ◽  
Initial Exposure ◽  
Fat Loss ◽  
Fat Depots ◽  
Siberian Hamsters ◽  
Total Body Fat ◽  
Total Body ◽  
Fat Pads

Siberian hamsters exhibit seasonal fluctuations in body weight (fat). Initial exposure to a short photoperiod results in body fat loss that reverses after approximately 22 wk of short-day exposure. The purpose of this study was to determine whether Siberian hamsters are able to recover body fat after surgical reduction of total lipid stores and if so, whether this ability is photoperiod dependent and fat pad specific. Either the largest pair of internal fat pads, the epididymal white adipose tissue (EWAT) or one pair of two large external depots, the inguinal (IWAT) fat pads, were removed from male hamsters housed for 22 wk in a long (LD) or short (SD) photoperiod. Retroperitoneal fat pad (RWAT) mass was increased in LD EWAT- and IWAT-lipectomized hamsters. IWAT mass also was increased in the LD EWAT-lipectomized hamsters. Neither SD-lipectomized group compensated for body fat loss in any of the measured fat pads. Increased food intake was not necessary for total body fat recovery, but undereating partially may be responsible for the lack of recovery in SDs. The results of these experiments demonstrate a photoperiod-dependent ability of male Siberian hamsters to regulate total body fat after partial lipectomy. In addition, recovery involves a fat pad-specific compensatory response to partial lipectomy, rather than a general increase in lipid deposition in all fat depots.

scholarly journals Variations in the Size of the Major Omentum Are Primarily Determined by Fat Cell Number

2013 ◽  
Vol 98 (5) ◽  
pp. E897-E901 ◽  
Author(s):  
Peter Arner ◽  
Daniel Peter Andersson ◽  
Anders Thörne ◽  
Mikael Wirén ◽  
Johan Hoffstedt ◽  
...  
Keyword(s):  
Computerized Tomography ◽  
Body Fat ◽  
Cell Number ◽  
Dual Energy ◽  
Adipocyte Size ◽  
Fat Cell ◽  
X Ray ◽  
The Metabolic Syndrome ◽  
Total Body Fat ◽  
Total Body

Objective: Accumulation of visceral adipose tissue (VAT) is strongly linked to insulin resistance. Variations in the size of any adipose depot are determined by alterations in adipocyte volume and/or number. The individual contribution of each of the latter factors was determined in the major omentum, a fully resectable VAT depot. Subjects: Total removal of the major omentum (omentectomy) was performed in conjunction with bariatric surgery in 55 obese patients. Tissue weight as well as mean adipocyte size and number in the omentum were determined. In subgroups, total VAT was estimated by computerized tomography (n = 17) or dual-energy x-ray absorptiometry (n = 34). Results: The weight of the major omentum (on average 0.6 kg) correlated significantly with total VAT mass estimated by computerized tomography or dual-energy x-ray absorptiometry (r = 0.48-0.7; P &lt; .01). Omental weight in relation to total body fat correlated with several features of the metabolic syndrome and inversely with serum-leptin (P &lt; .001). Mean adipocyte size and total adipocyte number correlated strongly with omental weight (r = 0.6-0.8; P &lt; .0001), irrespective of body mass index and total body fat mass, and accounted almost in total for interindividual variations in omental size. However, stepwise regression analysis demonstrated that adipocyte number was significantly (P &lt; .0001) more important (62%) than adipocyte size (35%). Conclusion: The size of the major omentum is representative for VAT mass and correlates with a pernicious metabolic profile. Variations in omental weight are primarily determined by adipocyte number and to a lesser degree by adipocyte size, suggesting that increased VAT mass in obesity is predominantly dependent on adipocyte proliferation.

Changes in body composition relative to weight and maturity of Australian Dorset Horn rams and wethers. 3. Fat partitioning

1985 ◽  
Vol 40 (1) ◽  
pp. 129-134 ◽  
Author(s):  
R. M. Butterfield ◽  
J. M. Thompson ◽  
K. J. Reddacliff
Keyword(s):  
Body Fat ◽  
Subcutaneous Fat ◽  
Live Weight ◽  
Fat Depots ◽  
Mesenteric Fat ◽  
Total Body Fat ◽  
Intermuscular Fat ◽  
The Mean ◽  
Total Fat ◽  
Total Body

ABSTRACTThe effect of castration on fat partitioning in mature animals and on the maturing patterns for fat depots relative to total body fat was examined using slaughter and dissection data from 20 Dorset Horn rams and 20 Dorset Horn wethers. Animals were slaughtered at 6 kg intervals from 18 kg live weight to maturity. Five rams and eight wethers were classified as mature.In the mature animals the partitioning of fat differed for the rams and wethers in that the rams had a lower proportion of subcutaneous fat, and higher proportion of intermuscular and mesenteric fat than the wethers. However, the proportions of total carcass dissectible fat (i.e. subcutaneous plus intermuscular fat) and of the total non-carcass depots (i.e. kidney plus channel fat, omental, scrotal and thoracic fat) did not significantly differ between rams and wethers.The maturity coefficients of individual fat depots of rams and wethers were not significantly different and six of the nine depots were average maturing relative to total body fat. The intermuscular and thoracic fat depots were early maturing (maturity coeffient q= 1·19, 2·26 respectively), and the omental depot was late maturing (q= 0·52).Comparison of the partitioning of fat in rams and wethers reflected the differences in the mature animals when made at either the mean weight or at the mean stage of maturity, as the maturing patterns of most depots were not greatly different from that of total fat.

scholarly journals The key role of a glucagon-like peptide-1 receptor agonist in body fat redistribution

2019 ◽  
Vol 240 (2) ◽  
pp. 271-286 ◽  
Author(s):  
Li Zhao ◽  
Chunfang Zhu ◽  
Meng Lu ◽  
Chi Chen ◽  
Xiaomin Nie ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Body Fat ◽  
Visceral Fat ◽  
Subcutaneous Fat ◽  
Control Group ◽  
Acid Oxidation ◽  
High Dose ◽  
Fat Depots ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Glucagon-like peptide-1 receptor agonists (GLP-1RAs) are an ideal therapy for type 2 diabetes and, as of recently, for obesity. In contrast to visceral fat, subcutaneous fat appears to be protective against metabolic diseases. Here, we aimed to explore whether liraglutide, a GLP-1RA, could redistribute body fat via regulating lipid metabolism in different fat depots. After being fed a high-fat diet for 8 weeks, 50 male Wistar and Goto-Kakizaki rats were randomly divided into a normal control group, a diabetic control group, low- and high-dose liraglutide-treated groups and a diet-control group. Different doses of liraglutide (400 μg/kg/day or 1200 μg/kg/day) or an equal volume of normal saline were administered to the rats subcutaneously once a day for 12 weeks. Body composition and body fat deposition were measured by dual-energy X-ray absorptiometry and MRI. Isotope tracers were infused to explore lipid metabolism in different fat depots. Quantitative real-time PCR and Western blot analyses were conducted to evaluate the expression of adipose-related genes. The results showed that liraglutide decreased visceral fat and relatively increased subcutaneous fat. Lipogenesis was reduced in visceral white adipose tissue (WAT) but was elevated in subcutaneous WAT. Lipolysis was also attenuated, and fatty acid oxidation was enhanced. The mRNA expression levels of adipose-related genes in different tissues displayed similar trends after liraglutide treatment. In addition, the expression of browning-related genes was upregulated in subcutaneous WAT. Taken together, the results suggested that liraglutide potentially redistributes body fat and promotes browning remodeling in subcutaneous WAT to improve metabolic disorders.

Compensation for partial lipectomy in mice with genetic alterations of leptin and its receptor subtypes

2002 ◽  
Vol 283 (5) ◽  
pp. R1094-R1103 ◽  
Author(s):  
Ruth B. S. Harris ◽  
Dorothy B. Hausman ◽  
Timothy J. Bartness
Keyword(s):  
Body Fat ◽  
Short Form ◽  
Genetic Alterations ◽  
Cell Number ◽  
Feedback Signal ◽  
Receptor Subtypes ◽  
Wild Type ◽  
Leptin Receptors ◽  
Total Body Fat ◽  
Total Body

One hypothesis for the regulation of total body fat suggests that leptin is a lipostatic feedback signal that acts at brain sites involved in regulation of energy balance. The importance of leptin in recovery from partial surgical lipectomy was tested by performing bilateral epididymal lipectomy or sham surgery on wild-type and leptin-deficient ob/ob mice. Eight weeks later, nonexcised pads of lipectomized mice were increased but total carcass fat was lower than in sham-operated ob/ob mice. In experiment 2, ob/ob mice, wild-type mice, and two db/db mutants, C57BL/6J dbLepr/dbLepr (BL/6J) mice possessing short-form and circulating leptin receptors and C57BL/6J db3J/db3J (BL/3J) mice expressing only circulating receptors, were lipectomized or sham operated. Sixteen weeks later, body mass and carcass lipid were not different between sham and lipectomized ob/ob mice, wild-type mice, or BL/6J db/db mice, whereas there was incomplete (decreased carcass fat) but suggestive recovery (increased retroperitoneal fat mass and cell number) in lipectomized BL/3J db/db mice. These data indicate that leptin is not required for the regulation of total body fat.

Regional differences in fat pad responses to short days in Siberian hamsters

1989 ◽  
Vol 257 (6) ◽  
pp. R1533-R1540 ◽  
Author(s):  
T. J. Bartness ◽  
J. M. Hamilton ◽  
G. N. Wade ◽  
B. D. Goldman
Keyword(s):  
Body Weight ◽  
Body Fat ◽  
Subcutaneous Fat ◽  
Short Photoperiod ◽  
Fat Pad ◽  
Initial Exposure ◽  
Short Day ◽  
Siberian Hamsters ◽  
Long Day ◽  
Induced Changes

Siberian hamsters exhibit decreased body weight and fat after initial exposure to short photoperiods and increased body weight and fat after extended short photoperiod exposure. The purpose of the present experiments was to determine if uniform changes in white adipose tissue (WAT) pad weights and lipid metabolism correspond to these short photoperiod-induced changes in body fat. Carcass lipid content and testes and fat pad weights [retroperitoneal WAT (RWAT), epididymal WAT (EWAT), and inguinal and dorsal subcutaneous WAT, respectively] were decreased in male hamsters relative to their long day counterparts after 6 and 12 wk of short-day exposure. Moreover, EWAT and RWAT weight, EWAT specific lipoprotein lipase activity, and specific and total lipogenesis were disproportionately decreased relative to the subcutaneous fat pads. The changes in fat pad weight and metabolism were generally reversed coincident with the return to a long-day-like reproductive status after prolonged short-day exposure (24 and 30 wk). In a less detailed experiment, female Siberian hamsters had decreased body, fat pad, and uterine weights after 6 wk of short-day exposure; however, no fat pad-specific changes in weight were observed. The results of these experiments demonstrate that short-day-exposed male Siberian hamsters may be a useful model for examining mechanisms underlying fat pad-specific responses. In addition, gender appears to influence the pattern of short-day-induced lipid depletion in this species.

White adipose tissue sensory nerve denervation mimics lipectomy-induced compensatory increases in adiposity

2005 ◽  
Vol 289 (2) ◽  
pp. R514-R520 ◽  
Author(s):  
Haifei Shi ◽  
Timothy J. Bartness
Keyword(s):  
Adipose Tissue ◽  
Body Fat ◽  
White Adipose Tissue ◽  
Sensory Nerve ◽  
Sensory Innervation ◽  
Siberian Hamsters ◽  
Total Body Fat ◽  
Total Body ◽  
Lipid Stores ◽  
The Brain

The sensory innervation of white adipose tissue (WAT) is indicated by the labeling of sensory bipolar neurons in the dorsal root ganglion after retrograde dye placement into WAT. In addition, immunoreactivity (ir) for sensory-associated neuropeptides such as calcitonin gene-related peptide (CGRP) and substance P in WAT pads also supports the notion of WAT sensory innervation. The function of this sensory innervation is unknown but could involve conveying the degree of adiposity to the brain. In tests of total body fat regulation, partial surgical lipectomy triggers compensatory increases in the mass of nonexcised WAT, ultimately resulting in restoration of total body fat levels in Siberian hamsters and other animals. The signal that triggers this compensation is unknown but could involve disruption of WAT sensory innervation that accompanies lipectomy. Therefore, a local and selective sensory denervation was accomplished by microinjecting the sensory nerve neurotoxin capsaicin bilaterally into epididymal WAT (EWAT) of Siberian hamsters, whereas controls received vehicle injections. Additional hamsters had bilateral EWAT lipectomy (EWATx) or sham lipectomy. As seen previously, EWATx resulted in significantly increased retroperitoneal WAT (RWAT) and inguinal WAT (IWAT) masses. Capsaicin treatment significantly decreased CGRP- but not tyrosine hydroxylase-ir, attesting to the diminished and selective sensory innervation. Capsaicin-treated hamsters also had increased RWAT and, to a lesser degree, IWAT mass largely mimicking the WAT mass increases seen after lipectomy. Collectively, these data suggest the possibility that information related to peripheral lipid stores may be conveyed to the brain via the sensory innervation of WAT.

scholarly journals Genetic and Pharmacological Inhibition of PAPP-A Protects Against Visceral Obesity in Mice

Endocrinology ◽  
2020 ◽  
Vol 161 (10) ◽  
Author(s):  
Akhila Ramakrishna ◽  
Laurie K Bale ◽  
Sally A West ◽  
Cheryl A Conover
Keyword(s):  
Gene Expression ◽  
Adipose Tissue ◽  
Liver Lipid ◽  
Protein A ◽  
Visceral Obesity ◽  
Cell Number ◽  
Metabolic Effects ◽  
Adipocyte Size ◽  
Fat Depots ◽  
Mesenteric Fat

Abstract Pathogenicity of visceral adipose tissue (VAT) has been linked to the metabolic stress of enlarging mature adipocytes and a limited ability to recruit new adipocytes. One of the major distinguishing features of VAT preadipocytes is the high expression of the zinc metalloprotease, pregnancy-associated plasma protein-A (PAPP-A), when compared to subcutaneous adipose tissue (SAT). In this study we used 2 different approaches to investigate the effect of PAPP-A inhibition on different fat depots in mice on a high-fat diet (HFD) for 15 weeks. Conditional knockdown of PAPP-A gene expression in female adult mice resulted in significant decreases of 30% to 40% in adipocyte size in VAT (mesenteric and pericardial depots) compared to control mice. There was no effect on SAT (inguinal) or intra-abdominal perigonadal fat. Liver lipid was also significantly decreased without any effect on heart and skeletal muscle lipid. We found similar effects when using a pharmacological approach. Weekly injections of a specific immunoneutralizing monoclonal antibody (mAb-PA 1/41) or isotype control were given to male and female wild-type mice on HFD for 15 weeks. Adipocyte size was significantly decreased (30%-50%) only in VAT with mAb-PA 1/41 treatment. In this model, cell number was significantly increased in mesenteric fat in mice treated with mAb-PA 1/41, suggesting hyperplasia along with reduced hypertrophy in this VAT depot. Gene expression data indicated a significant decrease in F4/80 (macrophage marker) and interleukin-6 (proinflammatory cytokine) and a significant increase in adiponectin (anti-inflammatory adipokine with beneficial metabolic effects) in mesenteric fat compared to inguinal fat in mice treated with mAb-PA 1/41. Furthermore, there was significantly decreased liver lipid content with mAb-PA 1/41 treatment. Thus, using 2 different models systems we provide proof of principle that PAPP-A inhibition is a potential therapeutic target to prevent visceral obesity and its metabolic sequelae, such as fatty liver.

ON BODY FAT AND BODY WATER IN RATS

1955 ◽  
Vol 33 (1) ◽  
pp. 970-979 ◽  
Author(s):  
Louis-Marie Babineau ◽  
Edouard Pagé
Keyword(s):  
Adipose Tissue ◽  
Body Fat ◽  
Dry Matter ◽  
The Body ◽  
Energy Reserves ◽  
Experimental Conditions ◽  
Fat Depots ◽  
Excess Water ◽  
Fat Stores ◽  
Free Body

Under our experimental conditions, water represented 72% of the fat-free body mass. This constant was found to be completely independent of the magnitude of the fat depots. Consideration of the composition of various samples of adipose tissue suggests that the water to fat-free dry matter ratio is the same as in the body as a whole or that any "excess" water contributed by adipose tissue is so small in absolute amounts as to leave the global ratio essentially undisturbed. Rats exposed to cold had to draw on their fat stores during the first month of exposure but later replenished their energy reserves. The water to fat-free dry matter ratio was not affected.

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