Enzyme-linked immunosorbent assay of colostral IgG transported into lymph and plasma in neonatal pigs

1992 ◽  
Vol 263 (4) ◽  
pp. R976-R980
Author(s):  
H. Kiriyama
Keyword(s):  
Blood Flow ◽  
Small Intestine ◽  
Immunosorbent Assay ◽  
Lymph Flow ◽  
Enzyme Linked Immunosorbent Assay ◽  
Maximal Level ◽  
Neonatal Pigs ◽  
Duodenal Lumen ◽  
Ig G ◽  
Plateau Level

Amounts of colostral proteins in lymph and plasma were estimated by an enzyme-linked immunosorbent assay (ELISA) after infusion of bovine colostrum into the duodenal lumen in nonsuckling neonatal pigs. The rate of immunoglobulin (Ig) G transport in lymph of the thoracic duct reached the maximal level (4.7 +/- 1.3 mg.15 min-1.kg body wt-1) within 3 h after the duodenal infusion. The rate of small protein (SP) transport more slowly increased than that of IgG. On the contrary, casein remained in the much lower level in lymph. IgG concentration in plasma increased gradually and reached a plateau level (2.5 +/- 0.9 g/l) 5 h after the infusion, but the levels of SP and casein were slowly and slightly increased in plasma. The IgG-to-casein and SP-to-casein ratios in lymph and plasma were 161:15:1 and 128:12:1 4 h after the infusion, respectively, and much higher than the value in the colostrum (IgG/SP/casein = 15:4:1). These results indicate 1) that IgG transported via lymph flow after absorption through the small intestine is faster than that via blood flow, 2) that the concentration of absorbed IgG is higher than that of absorbed SP and much higher than the concentration of absorbed casein both in the lymph and plasma, and 3) that total amount of colostral protein transported via blood flow is larger than that transported via lymph flow.

scholarly journals Polyacrylamide Gel Electrophoresis of Proteins Method versus Enzyme-Linked Immunosorbent Assay and Immunochromatography with Monoclonal Antibodies in Pediatric Infection with Helicobacter Pylori

2020 ◽  
Vol 71 (4) ◽  
pp. 446-455
Author(s):  
Raluca Suzana Iftimi ◽  
Ana Maria Alexandra Stanescu ◽  
Carmen Anton ◽  
Smaranda Diaconescu ◽  
Nicoleta Gimiga ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Monoclonal Antibodies ◽  
Western Blot ◽  
Immunosorbent Assay ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Enzyme Linked Immunosorbent Assay ◽  
Western Blot Method ◽  
Ig G

The current methods for the diagnosis of Helicobacter pylori infection in children include invasive (direct) methods, that enable the detection of the bacteria or the bacterial urease in gastric biopsies, or noninvasive (indirect) methods, which consist of researching specific antibodies, antigens and urease in other different samples (serum, saliva, urine, stool, exhaled air). The urease functions in H. pylori infection is to neutralize gastric acid by producing NH3 through the hydrolysis of urea. Monochloramine, a NH3-derived compounds has cytotoxic effects on host cells. The Western Blot method refers to the extraction of a a sodium dodecyl sulphate from a Helicobacter pylori strain followed by a separation of the solubilised protein using discontinous polyacrylamide gel electrophoresis according to molecular mass and transfer of the separated proteins to nitrocellulose. The ELISA (enzyme-linked immunosorbent assay) technique refers to the detection of Anti-Helicobacter pylori antibodies type Ig G. We also used an immunochromatographic system with the lateral leakage test strip based on the principle of the sandwich technique with monoclonal antibodies. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold �labelled antibodies and complementary oligonucleotide-labelled immobilized at different places on a nitrocellulose membrane. Our study aims to assess the performances of the Western Blot method for determining anti-Helicobacter pylori Ig A and Ig G antibodies in correlation with clinical data and other available diagnosis methods. For validation purposes, the results were compared to those obtained using the enzyme-linked immunosorbent assay technique, gastric biopsy and immunochromatography.

Ileal microvascular permeability: a comparison of PS and sigma d using lymphatic and isotopic protein clearances

1992 ◽  
Vol 262 (3) ◽  
pp. G572-G580 ◽  
Author(s):  
M. I. Townsley ◽  
S. M. Smith ◽  
R. K. Reed ◽  
V. H. Pitts ◽  
A. E. Taylor
Keyword(s):  
Blood Flow ◽  
Venous Pressure ◽  
Lymph Flow ◽  
Control Group ◽  
Reflection Coefficients ◽  
Additional Group ◽  
Permeability Surface ◽  
Albumin Clearance ◽  
D Values ◽  
Ig G

Lymphatic clearances of total protein, albumin, immunoglobulin (Ig) G, and IgM obtained in autoperfused cat ileum as venous pressure (Pv) was elevated were analyzed using traditional methods as well as the newer maximal diffusion (max,diff) method of Reed et al. [Am. J. Physiol. 261 (Heart Circ. Physiol. 30): H728-H740, 1991; Am. J. Physiol. 257 (Heart Circ. Physiol. 26): H1037-H1041, 1989] to determine capillary osmotic reflection coefficients (sigma d) and unique permeability surface area products (PS). In a control group (n = 6), sigma d max,diff and PSmax,diff for albumin, IgG, and IgM could not be determined due to their transiently high clearance patterns. These patterns were not altered by treatment with vasodilators (n = 6) to prevent redistribution of blood flow from the mucosal-submucosal to muscularis layer of the ileum, which accompanies elevation of ileal Pv. Furthermore, sigma d values for IgG in both groups were lower than those for both albumin and IgM, a finding inconsistent with predictions based on pore theory, together suggesting that lymphatic protein clearances were influenced by washout of interstitial proteins, a conclusion supported by the decrease in tissue content of albumin and IgG after elevation of Pv. Clearances of radiolabeled albumin (n = 10) and IgG (n = 6), measured after an initial 2-h steady-state period at a constant Pv, were markedly reduced at low lymph flow compared with those obtained in the shorter term experiments. In an additional group (n = 5), albumin clearance peaked at 10 times baseline within 15 min after a step increase in Pv to 30 mmHg but fell to only 3 times baseline within 1 h.(ABSTRACT TRUNCATED AT 250 WORDS)

Serum Feline Pancreatic Lipase Immunoreactivity Concentration and Seroprevalences of Antibodies Against Toxoplasma Gondii and Bartonella Species in Client-Owned Cats

2009 ◽  
Vol 11 (8) ◽  
pp. 663-667 ◽  
Author(s):  
Danielle B. Bayliss ◽  
Jörg M. Steiner ◽  
Jan S. Sucholdolski ◽  
Steven V. Radecki ◽  
Melissa M. Brewer ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Pancreatic Lipase ◽  
Clinical Findings ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Bartonella Species ◽  
Serum Samples ◽  
Serological Tests ◽  
Ig G

Feline pancreatitis is a commonly suspected illness and it has been proposed that some cases of feline pancreatitis may be caused by infection with Toxoplasma gondii or Bartonella species. Feline pancreatic lipase immunoreactivity (fPLI) is a test performed on serum that is commonly combined with other clinical findings as an indirect aid in the diagnosis of pancreatitis. The purpose of this study was to determine if there are associations between fPLI concentration and the presence of serum antibodies against T gondii or Bartonella species. Serum samples from 458 cats, for which serum fPLI concentrations had already been determined, were assayed by enzyme-linked immunosorbent assay for the presence of T gondii immunoglobulin (Ig) G (IgG) and IgM antibodies, and Bartonella species IgG antibodies. The association between fPLI concentration and T gondii or Bartonella species antibodies was determined. No statistically significant association was found between fPLI concentration and T gondii or Bartonella species antibodies, suggesting that serological tests for the organisms are not useful in cases with increased fPLI concentration.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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