Continuous measurement of protein osmotic pressure in blood and lymph of sheep

1988 ◽  
Vol 64 (2) ◽  
pp. 869-873 ◽  
Author(s):  
S. Yamada ◽  
M. Grady ◽  
N. C. Staub
Keyword(s):  
Osmotic Pressure ◽  
Total Protein ◽  
Protein Concentration ◽  
Continuous Measurement ◽  
Excellent Correlation ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Continuous Measurements ◽  
The Relationship

We have continuously measured protein osmotic pressure of blood and lymph in sheep to compare two kinds of needle osmometers (rigid and flexible) with a membrane osmometer (Wescor). We also compared the averaged values of the continuous measurement with osmotic pressure calculated from total protein and albumin fraction, using the Yamada equation. The rigid-needle and membrane osmometers showed excellent correlation (y = 1.00x + 0.06; r greater than 0.99). The flexible-needle osmometer tended to overestimate osmotic pressure (avg 16%). We used the rigid-needle osmometer for continuous measurements of protein osmotic pressure of blood and lymph in anesthetized or unanesthetized sheep to observe changes in protein osmotic pressure of blood and lymph through the three different interventions. The relationship between the theoretical values (x) and the continuous measurements (y) of osmotic pressure was good (y = 0.99x + 0.16, r = 0.97), but after various interventions, the continuously measured protein osmotic pressure tended to exceed the calculated measurements. The continuous measurement should be monitored with spot samples measured in a stationary osmometer or by calculation of osmotic pressure from total protein concentration and albumin fraction.

Relationship between colloid osmotic pressure and plasma protein concentration in the dog

1977 ◽  
Vol 233 (2) ◽  
pp. H295-H298 ◽  
Author(s):  
P. D. Navar ◽  
L. G. Navar
Keyword(s):  
Osmotic Pressure ◽  
Total Protein ◽  
Protein Concentration ◽  
Colloid Osmotic Pressure ◽  
Plasma Samples ◽  
Total Protein Concentration ◽  
Plasma Protein Concentration ◽  
Dog Plasma ◽  
The Relationship ◽  
Pressure Analysis

This study was done to establish the correct relationship between protein concentration and plasma colloid osmotic pressure in the dog and to determine the possible influence of the relative albumin and globulin content (A:G ratio). Plasma samples from dogs, rats, and humans were evaluated for total protein concentration, globulin concentration, and colloid osmotic pressure. Samples were concentrated and diluted by ultrafiltration to provide a range of total protein concentrations from 1 to 12 g/dl. Rat and human plasma samples had A:G ratios of 1.4 and 2.1, respectively, and the relationship between protein concentration and colloid osmotic pressure was in agreement with the Landis-Pappenheimer equation. In contrast, dog plasma samples consistently exhibited lower colloid osmotic pressures for any given protein concentration. Two forms of empirical equations were derived to relate these parameters in the dog. Dog plasma samples had higher concentrations of globulin and the A:G ratio averaged 0.59 +/- 0.35 SD. There was a significant relationship between the A:G ratio and the plasma colloid osmotic pressure. Analysis of the possible effect of this altered relationship on glomerular filtration dynamics predicted that efferent plasma colloid osmotic pressure was not specifically affected and was dependent only on the filtration fraction and the plasma colloid osmotic pressure.

Albumin fraction and measurement of total protein concentration

1993 ◽  
Vol 264 (5) ◽  
pp. H1723-H1726 ◽  
Author(s):  
B. T. Peterson ◽  
R. W. Tate
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Gamma Globulin ◽  
Colorimetric Assay ◽  
Full Range ◽  
Computational Method ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Standard Curve ◽  
Protein Assays

The standard curve of a typical colorimetric assay for total protein is often nonlinear and dependent on the albumin fraction of the protein standard. We developed a simple mathematical transformation to make the standard curve linear and a computational method to correct for differences in albumin concentrations among the samples. This method uses data from total protein assays on two sets of standards (albumin and gamma globulin) and provides accurate measures of total protein over the full range of albumin fractions. Comparison of this two-standard method with the a method that uses only albumin as a standard shows that this method prevents physiologically significant overestimations in total protein concentration and calculated protein osmotic pressure differences in the lungs.

scholarly journals THE EFFECT OF FASTING ON THE SERUM PROTEIN CONCENTRATION OF THE RAT

1935 ◽  
Vol 62 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Harold C. Torbert
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Protein Fraction ◽  
Protein Concentration ◽  
Individual Resistance ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Large Numbers ◽  
Initial Drop ◽  
Serum Protein Concentration

1. Experiments were carried out to test the hypothesis that there exists a special circulating fraction of plasma protein available for use by the tissues. 2. The changes in serum protein concentration after varying periods of fasting were followed in large numbers of individual rats. 3. Previous reports from this laboratory of a small initial drop in the total protein concentration of the serum, with subsequent maintenance of the serum protein at the new level are confirmed. 4. Evidence is offered that this initial fall involves chiefly or solely the albumin fraction. 5. The mechanism responsible for the observed initial drop and subsequent maintenance of the protein is not exactly known, but two definite factors are age and individual resistance. 6. It is concluded that no satisfactory evidence is available to support the hypothesis of a directly utilizable protein fraction in the blood.

scholarly journals Mediation and Moderation Analysis of the Relationship between Total Protein Concentration and the Risk of Depressive Disorders in Older Adults with Function Dependence in Home Care

2018 ◽  
Author(s):  
Grzegorz Józef Nowicki ◽  
Barbara Ślusarska ◽  
Agnieszka Bartoszek ◽  
Katarzyna Kocka ◽  
Alina Deluga ◽  
...  
Keyword(s):  
Older Adults ◽  
Physical Function ◽  
Total Protein ◽  
Protein Concentration ◽  
Public Health Problem ◽  
Mediating Effect ◽  
Total Protein Concentration ◽  
Term Care ◽  
Function Impairment ◽  
The Relationship

Due to its devastating consequences, late life depression is an important public health problem. The aim of the study was the analysis of variables which may potentially influence risk of depression (GDS-SF). Furthermore, the aim was to study possible mediating effect of given variables on the relationship between the total protein concentration and risk of depression in older-adults with chronic diseases, and physical function impairment. The research sample included a total of 132 older adults with chronic conditions and physical function impairments, remaining under a long-term care in residential environment. Negative linear correlation was observed between patients’ physical functionality, total protein concentration, concentration of HDL cholesterol, arm circumference, and the risk of depression. Considerably stronger relationship was observed between total protein concentration, and GDS-SF, in elderly suffering from sensory dysfunction (b = −6.42, 95% CI = −11.27; −1.58). The effect of the mediation between depression risk is correlated to total protein concentration in blood serum, and the mediators are probably low function impairment and low levels of 25 (OH)D vitamin. Cohort control research is suggested to confirm the hypothesis.

scholarly journals Moderation and Mediation Analysis of the Relationship between Total Protein Concentration and the Risk of Depressive Disorders in Older Adults with Function Dependence in Home Care

Nutrients ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 1374
Author(s):  
Grzegorz Nowicki ◽  
Barbara Ślusarska ◽  
Agnieszka Bartoszek ◽  
Katarzyna Kocka ◽  
Alina Deluga ◽  
...  
Keyword(s):  
Older Adults ◽  
Physical Function ◽  
Total Protein ◽  
Protein Concentration ◽  
Public Health Problem ◽  
Total Serum ◽  
Sensory Organs ◽  
Total Protein Concentration ◽  
Term Care ◽  
The Relationship

Due to its devastating consequences, late-life depression is an important public health problem. The aim of the study was an analysis of variables which may potentially influence the risk of depression (GDS-SF). Furthermore, the aim was to study possible mediating effects of given variables on the relationship between the total protein concentration and the risk of depression in older adults with chronic diseases, and physical function impairment. The research sample included 132 older adults with chronic conditions and physical function impairments, residing in a long-term care in residential environment. In the studied group of sensory organs, diseases proved to be a significant moderator of the relationship between GDS-SF and total serum protein concentration. A stronger relationship was observed in subjects suffering from diseases of sensory organs (b = −6.42, 95% CI= −11.27; −1.58). The Barthel index and 25(OH)D vitamin were the most significant mediators of the examined relationship. Cohort research is suggested to confirm the hypothesis.

Determination of Total Protein Concentration in Solution Using Gold Electrode Modified with Silver Nanoparticles

2014 ◽  
Vol 27 (1) ◽  
pp. 253-257 ◽  
Author(s):  
Patrick Marcel Seumo Tchekwagep ◽  
Charles Péguy Nanseu-Njiki ◽  
Emmanuel Ngameni ◽  
Ravi Danielsson ◽  
Thomas Arnebrant ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Total Protein ◽  
Gold Electrode ◽  
Protein Concentration ◽  
Total Protein Concentration

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

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