scholarly journals Anticancer Potential of Aqueous Ethanol Seed Extract ofZiziphus mauritianaagainst Cancer Cell Lines and Ehrlich Ascites Carcinoma

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Tulika Mishra ◽  
Madhu Khullar ◽  
Aruna Bhatia
Keyword(s):  
Cell Lines ◽  
Ehrlich Ascites Carcinoma ◽  
Seed Extract ◽  
Ziziphus Mauritiana ◽  
Dependent Manner ◽  
Swiss Albino Mice ◽  
Ehrlich Ascites ◽  
Albino Mice

Ziziphus mauritiana(Lamk.) is a fruit tree that has folkloric implications against many ailments and diseases. In the present study, anticancer potential of seed extract ofZiziphus mauritiana in vitroagainst different cell lines (HL-60, Molt-4, HeLa, and normal cell line HGF) by MTT assay as well asin vivoagainst Ehrich ascites carcinoma bearing Swiss albino mice was investigated. The extract was found to markedly inhibit the proliferation of HL-60 cells. Annexin and PI binding of treated HL-60 cells indicated apoptosis induction by extract in a dose-dependent manner. The cell cycle analysis revealed a prominent increase in sub Go population at concentration of 20 μg/ml and above. Agarose gel electrophoresis confirmed DNA fragmentation in HL-60 cells after 3 h incubation with extract. The extract also exhibited potent anticancer potentialin vivo. Treatment of Ehrlich ascites carcinoma bearing Swiss albino mice with varied doses (100–800 mg/kg b.wt.) of plant extract significantly reduced tumor volume and viable tumor cell count and improved haemoglobin content, RBC count, mean survival time, tumor inhibition, and percentage life span. The enhanced antioxidant status in extract-treated animals was evident from decline in levels of lipid peroxidation and increased levels of glutathione, catalase, and superoxide dismutase.

Boric acid enhances in vivo Ehrlich ascites carcinoma cell proliferation in Swiss albino mice

Toxicology ◽  
2001 ◽  
Vol 165 (1) ◽  
pp. 1-11 ◽  
Author(s):  
S Qureshi ◽  
O.A Al-Shabanah ◽  
M.M Al-Harbi ◽  
A.M Al-Bekairi ◽  
M Raza
Keyword(s):  
Cell Proliferation ◽  
Boric Acid ◽  
Carcinoma Cell ◽  
Ehrlich Ascites Carcinoma ◽  
Ehrlich Ascites Carcinoma Cell ◽  
Swiss Albino Mice ◽  
Ehrlich Ascites ◽  
Albino Mice

Extract of Tagetes Erecta Could Be Used as a Potential Drug Candidate Against Cancer: A Study on the Anticancer Efficacy of Medicinal Plants Involving in Vitro and in Vivo Approach

2021 ◽  
Author(s):  
Dharmeswar Barhoi ◽  
Puja Upadhaya ◽  
Sweety Nath Barbhuiya ◽  
Anirudha Giri ◽  
Sarbani Giri
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Flow Cytometric Analysis ◽  
Cancer Cell Lines ◽  
Tagetes Erecta ◽  
Swiss Albino Mice ◽  
Albino Mice ◽  
Eac Cells

Abstract Globally, the burden of cancer is increasing consistently. Modern cancer therapies include lots of toxicity in the non-targeted organs reducing the life expectancy of the patients. Therefore, the development of safer alternative medicines with less toxicity and high efficacy is of immense importance. The present study was designed to evaluate the anticancer activity of a medicinal plant, “Tagetes erecta” (TE), in established cancer cell lines in vitro and in animal models in vivo. GC-MS analysis was performed that revealed hexadecanoic acid, Linolenic acid, Quinic acid, 2,3- dihydrobenzofuran (Coumaran), and β-stigmasterol as major bioactive compounds in TE leaves. Aqueous extract of Tagetes erecta (AETE) treatment potentially reduced the tumor weight (TW) and tumor volume (TV) and increased the life span in EAC-induced tumor-bearing Swiss albino mice. Side effect analysis confirmed the lack of toxicity of AETE to non-targeted organs in normal Swiss albino mice. Studies in cancer cell lines indicated dose and time-dependent cytotoxicity in Human laryngeal carcinoma (HEp-2) and Ehrlich ascites carcinoma (EAC) cells. Flow cytometric analysis established significant induction of apoptosis in EAC cells without arresting the cell cycle. In addition, AETE treatment led to a significant increase in cells with depolarised mitochondrial membrane potential. The present study indicated that AETE potentially inhibits tumor progression without disturbing normal body physiology. Thus, we conclude that AETE can be used as a potential therapeutic agent against cancer.

scholarly journals Anticancer activity of milk fat rich in conjugated linoleic acid against Ehrlich ascites carcinoma cells in female Swiss albino mice

2021 ◽  
Vol 14 (3) ◽  
pp. 696-708
Author(s):  
Abdelrahman M. Abd El-Gawad ◽  
Diea G. Abo El-Hassan ◽  
Ahmed M. Aboul-Enein ◽  
Sherein S. Abdelgayed ◽  
Salwa A. Aly ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Life Span ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Ehrlich Ascites Carcinoma ◽  
Swiss Albino Mice ◽  
Balanced Diet ◽  
Ehrlich Ascites ◽  
Albino Mice

Background and Aim: The major conjugated linoleic acid (CLA) isomers have anticancer effect, especially breast cancer cells, inhibits cell growth and induces cell death. Also, CLA has several health benefits in vivo, including antiatherogenesis, antiobesity, and modulation of immune function. The present study aimed to assess the safety and anticancer effects of milk fat CLA against in vivo Ehrlich ascites carcinoma (EAC) in female Swiss albino mice. This was based on acute toxicity study, detection of the tumor growth, life span of EAC bearing hosts, and simultaneous alterations in the hematological, biochemical, and histopathological profiles. Materials and Methods: One hundred and fifty adult female mice were equally divided into five groups. Groups (1-2) were normal controls, and Groups (3-5) were tumor transplanted mice (TTM) inoculated intraperitoneally with EAC cells (2×106/0.2 mL). Group (3) was (TTM positive control). Group (4) TTM fed orally on balanced diet supplemented with milk fat CLA (40 mg CLA/kg body weight). Group (5) TTM fed orally on balanced diet supplemented with the same level of CLA 28 days before tumor cells inoculation. Blood samples and specimens from liver and kidney were collected from each group. The effect of milk fat CLA on the growth of tumor, life span of TTM, and simultaneous alterations in the hematological, biochemical, and histopathological profiles were examined. Results: For CLA treated TTM, significant decrease in tumor weight, ascetic volume, viable Ehrlich cells accompanied with increase in life span were observed. Hematological and biochemical profiles reverted to more or less normal levels and histopathology showed minimal effects. Conclusion: The present study proved the safety and anticancer efficiency of milk fat CLA and provides a scientific basis for its medicinal use as anticancer attributable to the additive or synergistic effects of its isomers.

scholarly journals Synthesis and Evaluation of the Antitumor Activity of (E)-3-((2-(5-(4-chlorophenyl) thiazol-2-yl) hydrazono) methyl) benzene-1,2-diol “In vitro and In vivo Study”

2019 ◽  
pp. 1-16
Author(s):  
Faten Z. Mohamed ◽  
Mohamed S. Elghreeb ◽  
Moustafa S. Abdelhamid ◽  
Hazem A. Elbaz
Keyword(s):  
Antitumor Activity ◽  
Life Span ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cytotoxic Effect ◽  
Ehrlich Ascites Carcinoma ◽  
Ehrlich Ascites ◽  
Liver And Kidney

Background: Thiazole nucleus–containing compounds have an antitumor efficiency against various types of cancer. Purpose: The present study was designed to determine the cytotoxic effect of newly synthesized thiazole derivative (TD1) on human cancer cell lines, in addition to evaluate its antitumor activity against Ehrlich ascites carcinoma (EAC) in mice. Materials and Methods: TD1 was synthesized and investigated for its cytotoxic effect on HCT116 (colon cancer), HepG2 (liver cancer), PC3 (prostate cancer) and MCF7 (breast cancer). The effect of TD1 on cell viability, tumor volume, and percent of increase in life span (% ILS) in Ehrlich–bearing mice was studied. Hematological parameters, liver and kidney function tests were evaluated. The activity of superoxide dismutase (SOD) and catalase (CAT), as well as malondialdehyde (MDA) and reduced glutathione levels were determined in liver and kidney tissues. The expression of P53 in EAC was analyzed by flow cytometry. Results: TD1 demonstrated an inhibitory effect on both cancer cell lines in vitro and Ehrlich ascites cells in vivo. TD1 increased in life span of Ehrlich–bearing mice compared to control. Cell cycle and flow cytometric analysis revealed that TD1 directed Ehrlich cells toward apoptosis by increasing of P53 expression. Conclusion: It was concluded that TD1 have a potent antitumor activity against Ehrlich ascites carcinoma in mice beside a cytotoxic effect on MCF-7, PC3, HepG2 and HCT-116.

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