scholarly journals Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Hai-Yan Gui ◽  
Rui-Ni Chen ◽  
Yan Peng ◽  
Jin-Hua Hu ◽  
Zhao Mao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cytochrome P450 ◽  
Curcuma Longa ◽  
Mrna Level ◽  
Oxygen Species ◽  
Cytochrome P450 2E1 ◽  
Primary Mouse ◽  
Cyp2e1 Expression ◽  
Underlying Mechanisms ◽  
Expression And Activity

Curcumin is extracted from the rhizomes of the ginger family plantCurcuma longaL., which has a good protection for liver, kidney, and immune system. However, there is little information about its contribution in protection of astrocytes recently. The present study was undertaken to elucidate the protective effect of curcumin, an herbal antioxidant, on 1-methyl-4-phenylpyridinium ion- (MPP+-) and lipopolysaccharide- (LPS-) induced cytotoxicities, as well as the underlying mechanisms by using primary mouse mesencephalic astrocytes. The results showed that curcumin protected the mesencephalic astrocytes from MPP+- and LPS-induced toxicities along with reducing reactive oxygen species (P<0.05) and maleic dialdehyde (P<0.05) sufficiently. Moreover, curcumin significantly inhibited the cytochrome P450 2E1 (CYP2E1) expression (P<0.01at mRNA level,P<0.05at protein level) and its activity (P<0.05) sufficiently induced by MPP+and LPS in the mouse mesencephalic astrocytes. And curcumin as well as diallyl sulphide, a CYP2E1 positive inhibitor, ameliorated MPP+- and LPS-induced mouse mesencephalic astrocytes damage. Accordingly, curcumin protects against MPP+- and LPS-induced cytotoxicities in the mouse mesencephalic astrocyte via inhibiting the CYP2E1 expression and activity.

2,4-Dimethylaniline generates phosphorylated histone H2AX in human urothelial and hepatic cells through reactive oxygen species produced by cytochrome P450 2E1

2018 ◽  
Vol 92 (10) ◽  
pp. 3093-3101 ◽  
Author(s):  
Yonggang Qi ◽  
Tatsushi Toyooka ◽  
Hiroki Kashiwagi ◽  
Yukie Yanagiba ◽  
Shigeki Koda ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cytochrome P450 ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Cytochrome P450 2E1 ◽  
Histone H2ax ◽  
Hepatic Cells

scholarly journals Effects of Progesterone on Benzene Toxicity in Rats

2008 ◽  
Vol 59 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Yeshvandra Verma ◽  
Suresh Rana
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Cytochrome P450 ◽  
Reduced Glutathione ◽  
Female Rats ◽  
Oxygen Species ◽  
Cytochrome P450 2E1 ◽  
Antioxidative Effect ◽  
Cyp2e1 Activity

Effects of Progesterone on Benzene Toxicity in RatsBenzene is a frequently used industrial solvent. Its toxic manifestations could be modified by sex hormones, but mechanisms of their action are poorly understood. We have examined the influence of progesterone on lipid peroxidation (malondialdehyde), reduced glutathione (GSH), and cytochrome P450 2E1 (CYP2E1) in the liver and kidneys of female rats. Progesterone applied to benzene-treated rats inhibited the formation of reactive oxygen species (ROS), but in ovariectomised benzene-treated rats it significantly increased GSH in the liver. No improvement in CYP2E1 activity was observed in progesterone treated rats. Our results evidence that progesterone changes benzene toxicity (generation of ROS, oxidative stress). However, the probable antioxidative effect of progesterone needs to be confirmed by further studies.

scholarly journals Increased Cytotoxicity of Carbon Tetrachloride in a Human Hepatoma Cell Line Overexpressing Cytochrome P450 2E1

2002 ◽  
Vol 30 (4) ◽  
pp. 400-405 ◽  
Author(s):  
S Takahashi ◽  
T Takahashi ◽  
S Mizobuchi ◽  
M Matsumi ◽  
K Morita ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cytochrome P450 ◽  
Carbon Tetrachloride ◽  
Cell Line ◽  
Hepatoma Cell ◽  
Mrna Level ◽  
Hepatoma Cell Line ◽  
Cytochrome P450 2E1 ◽  
Hsp70 Mrna ◽  
Potential Marker

Cytotoxic free radicals generated during the metabolism of carbon tetrachloride by cytochrome P450 2E1 (CYP2E1) are thought to cause hepatotoxicity. Here, the cytotoxic effects of carbon tetrachloride in a liver cell line expressing CYP2E1 (HLE/2E1) are compared with those in the mother cell line (HLE). The effects of carbon tetrachloride on the gene expression of HSP70, a potential marker of oxidative stress, were also examined. The viability of HLE/2E1 cells after exposure to carbon tetrachloride was significantly decreased compared with that of HLE cells. Northern blot analysis revealed that the HSP70 mRNA level was significantly increased after carbon tetrachloride treatment in both cell lines, while the magnitude of its increase was much greater in HLE/2E1 cells than in HLE cells. These results suggest that the oxidative stress induced by CYP2E1 plays an important role in the increase in cytotoxicity of carbon tetrachloride in CYP2E1-overexpressing cells.

Curcuma longa extract reduces the risk of oxidative stress during intense physical exertion

2020 ◽  
pp. 43-49
Author(s):  
A. A. Khisamova ◽  
O. A. Gizinger
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Literature Review ◽  
Curcuma Longa ◽  
Antioxidant Properties ◽  
Physical Exertion ◽  
The Body ◽  
Irreversible Processes ◽  
Oxygen Species ◽  
Wide Range

Increased physical exertion is a catalyst for oxidative stress and the production of reactive oxygen species, which entails irreversible processes in the body, leading to chronic diseases and disability. This article contains a literature review of studies that prove the effect of the antioxidant properties of Curcuma longa on cells under oxidative stress. To search for data, a wide range of literature and databases was explored: Pubmed, Google.Scholar, and Embase.

scholarly journals Danshenol A Alleviates Hypertension-Induced Cardiac Remodeling by Ameliorating Mitochondrial Dysfunction and Suppressing Reactive Oxygen Species Production

2019 ◽  
Vol 2019 ◽  
pp. 1-18
Author(s):  
Kai Chen ◽  
Yiqing Guan ◽  
Yunci Ma ◽  
Dongling Quan ◽  
Jingru Zhang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Mitochondrial Dysfunction ◽  
Cardiac Remodeling ◽  
Cardiac Fibrosis ◽  
Spontaneously Hypertensive Rat ◽  
Cardiac Injury ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Spontaneously Hypertensive ◽  
Underlying Mechanisms

Current therapeutic approaches have a limited effect on cardiac remodeling, which is characteristic of cardiac fibrosis and myocardial hypertrophy. In this study, we examined whether Danshenol A (DA), an active ingredient extracted from the traditional Chinese medicine Radix Salviae, can attenuate cardiac remodeling and clarified the underlying mechanisms. Using the spontaneously hypertensive rat (SHR) as a cardiac remodeling model, DA ameliorated blood pressure, cardiac injury, and myocardial collagen volume and improved cardiac function. Bioinformatics analysis revealed that DA might attenuate cardiac remodeling through modulating mitochondrial dysfunction and reactive oxygen species. DA repaired the structure/function of the mitochondria, alleviated oxidative stress in the myocardium, and restored apoptosis of cardiomyocytes induced by angiotensin II. Besides, DA inhibited mitochondrial redox signaling pathways in both the myocardium and cardiomyocytes. Thus, our study suggested that DA attenuates cardiac remodeling induced by hypertension through modulating mitochondrial dysfunction and reactive oxygen species.

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