scholarly journals Benzophenone Suppression of Quercetin Antioxidant Activity towards Lipids under UV-B Irradiation Regime: Detection by HPLC Chromatography

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Jelena S. Stanojević ◽  
Dejan Z. Marković ◽  
Jelena B. Zvezdanović
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Spectrophotometric Method ◽  
Methanol Solution ◽  
The Other ◽  
Lipid Mixture ◽  
Irradiation Regime

Quercetin, a well-known flavonoid antioxidant, has been employed to control benzophenone-sensitized peroxidation of the lipid mixture in methanol solution, induced by continuous UV-B irradiation. Surprisingly, the detected quercetin antioxidant activity was almost negligible. The presented data suggests that the reason is not in its own UV-B-induced degradation but rather in its interrelationship with benzophenone during UV-B stress. On the other side of this relationship, benzophenone anticipated sensitizing role towards lipids; that is, the initiation of lipid peroxidation has been affected as well. These results, obtained by HPLC chromatography, partly confirm but partly relativize to some extent recent results obtained with the same system by spectrophotometric method.

scholarly journals UV-induced change in quercetin antioxidant activity toward benzophenone initiated lipid peroxidation

2012 ◽  
Vol 77 (11) ◽  
pp. 1571-1588 ◽  
Author(s):  
Jelena Zvezdanovic ◽  
Dejan Markovic ◽  
Dragan Cvetkovic ◽  
Jelena Stanojevic
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Uv Irradiation ◽  
Energy Input ◽  
Incident Light ◽  
Methanol Solution ◽  
Suppression Effect ◽  
Peroxidation Processes ◽  
Uv Photons ◽  
Uv C

The aim of this work is to estimate the degradation and change in antioxidant activity of quercetin in the presence of two different mixtures of phospholipids in methanol solution, under continuous UV-irradiation from three different sub-ranges (UV-A, UV-B and UV-C), in the presence and in the absence of selected UV-absorbing photosensitizer, benzophenone. Quercetin is employed to control lipid peroxidation process generated by UV-irradiation, by absorbing part of the UV-incident light, or/and by scavenging the involved, created free radicals. The results show that quercetin undergoes to irreversible destruction, highly dependent on UV-photons energy input, more expressed in the presence than in the absence of benzophenone. In the same time quercetin expresses suppression effect on lipid peroxidation processes in UV-irradiated phospholipid mixtures in both cases - absence or presence of benzophenone (more or less effective, respectively). In UV-C-irradiated mixtures, benzophenone photosensitizing function is significantly reduced due to its strong absorption in the same UV-C spectral range, therefore affecting less antioxidant activity of the remained quercetin.

Antioxidant Activity of Resveratrol Compared with Common Food Additives

2001 ◽  
Vol 64 (3) ◽  
pp. 379-384 ◽  
Author(s):  
M. ANTONIA MURCIA ◽  
MAGDALENA MARTÍNEZ-TOMÉ
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Sodium Tripolyphosphate ◽  
Food Additives ◽  
The Other ◽  
Butylated Hydroxytoluene ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Scavenger Activity ◽  
Inhibit Lipid Peroxidation

Resveratrol is a phenolic compound of the stilbene family present in wines and various parts of the grape, including the skin. In this study, the antioxidant and prooxidant activities of resveratrol were compared with other antioxidants (butylated hydroxytoluene [BHT], butylated hydroxyacetone [BHA], phenol, propyl gallate [PG], sodium tripolyphosphate [TPP], α-tocopherol, and vanillin) widely used in foods. The ability of these compounds to inhibit lipid peroxidation was as follows: BHA > resveratrol > PG > tripolyphosphate > vanillin > phenol > BHT > α-tocopherol, the first three inhibiting the peroxidation in a concentration-dependent manner. The order of OH scavenger activity of the tested compounds was BHA > TPP > BHT. Resveratrol and vanillin produced between 10 to 7% and 16 to 10% inhibition of the deoxyribose attack, respectively, but they do not scavenge OH▪. Neither the resveratrol analyzed nor PG or the rest of compounds reacted with H2O2 and must be considered inefficient in catalyzing any subsequent oxidation. The ability to scavenge HOCl was, in decreasing order, PG > resveratrol > α-tocopherol > phenol. The other compounds did not scavenge HOCl.

Comparative Phytochemical Research of Solidago Genus: S. graminifolia. Note I. Flavonoids

2018 ◽  
Vol 1 (1) ◽  
pp. 18-26
Author(s):  
Luciana Dobjanschi ◽  
Ramona Păltinean ◽  
Laurian Vlase ◽  
Mihai Babotă ◽  
Luminita Fritea ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Biological Properties ◽  
The Other ◽  
Indigenous Species ◽  
Solidago Canadensis ◽  
Phytochemical Composition ◽  
Chromatographic Profile ◽  
Solidago Virgaurea

Abstract Solidago graminifolia L. Salisb. is one of the latest species appeared in Romania. Due to the interest for the Solidago species as medicinal plants, we researched its phytochemical composition in comparison with the other three species present in Romania: Solidago virgaurea L., Solidago canadensis L., Solidago gigantea Aiton. Starting from the chemotaxonomic value of flavonoids, and their valuable biological properties, we wanted to analyze these substances from S. graminifolia compared to other Solidago species in Romanian flora. The studied species have a high content of flavonoids (3.44-5.21%). The flavonoid substances identified in the indigenous species of Solidago have a high chemotaxonomic value, each species having a charateristic chromatographic profile, therefore their analysis is useful in the case of adulterations. The qualitative analysis of flavonoids was performed by TLC and HPLC-MS, mean while the quantitative determination was achieved by spectrophotometric method and individual fractions separated by HPLC-MS.

scholarly journals Antioxidant activity of rice plants sprayed with herbicides

2016 ◽  
Vol 46 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Marcos André Nohatto ◽  
Dirceu Agostinetto ◽  
Ana Claudia Langaro ◽  
Claudia de Oliveira ◽  
Queli Ruchel
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Acetolactate Synthase ◽  
Defense Behavior ◽  
Sod Activity ◽  
Rice Plants ◽  
Synthase Inhibitor ◽  
Acetyl Coenzyme A Carboxylase ◽  
Lower Capacity

ABSTRACT Understanding the physiological defense behavior of plants subjected to herbicide application may help to identify products with higher or lower capacity to cause oxidative stress in crops. This study aimed at evaluating the effect of herbicides in the antioxidant activity of rice plants. The experimental design was completely randomized, with six replications. Treatments consisted of the herbicides bentazon (photosystem II inhibitor; 960 g ha-1), penoxsulam (acetolactate synthase inhibitor; 60 g ha-1), cyhalofop-butyl (acetyl coenzyme-A carboxylase inhibitor; 315 g ha-1) and a control. After the herbicides application, samples of rice shoots were collected at 12, 24, 48 and 96 hours after application (HAA). The components evaluated were hydrogen peroxide (H2O2), lipid peroxidation and activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Bentazon (up to 24 HAA) and penoxsulam (48 and 96 HAA) reduced the CAT activity. Moreover, these herbicides increased the levels of H2O2, lipid peroxidation and SOD activity, indicating a condition of oxidative stress in rice plants. The cyhalofop-butyl herbicide did not alter the antioxidant activity, showing that it causes less stress to the crop.

scholarly journals Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem

2021 ◽  
pp. 1-9
Author(s):  
Gerard Bessan Dossou-Agoin ◽  
Habib Ganfon ◽  
Fidèle Assogba ◽  
Adam Gbankoto ◽  
Joachim Gbenou ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Aqueous Extracts ◽  
Fruit Extract ◽  
Stem Extract ◽  
Dpph Scavenging

Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract. Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.

scholarly journals New Mixed Ligand Cobalt(II), Nickel(II) and Copper(II) Complexes of 2,2'-Bipyridine-3,3'-Dicarboxylic acid (bpdc) with 2-Mercapto-5-Phenyl-1,3,4-Oxadiazole (phozSH) and Their Antioxidant activity

2020 ◽  
Vol 36 (05) ◽  
pp. 834-842
Author(s):  
Rezan Ali Saleh ◽  
Hikmat Ali Mohammad ◽  
Salim Najm Aldin Saber
Keyword(s):  
Antioxidant Activity ◽  
Metal Ions ◽  
Dicarboxylic Acid ◽  
Potassium Hydroxide ◽  
Electronic Spectroscopy ◽  
Conductivity Measurement ◽  
Mixed Ligand ◽  
Methanol Solution ◽  
Dpph Method ◽  
Colored Complexes

The mixing of one mole of 2,2'-bipyridine-3,3'-dicarboxylic acid (bpdc) with two mole of potassium hydroxide (KOH) in methanol were refluxed for (half hour), followed by addition of one mole methanol solution of MCl2.nH2O (where M=Co, Ni or Cu). The mixture was refluxed for (2 hours) to give colored complexes of the metal ions of [M(bpdc)(H2O)4]. The [M(bpdc)(H2O)4] were reacted with one mole of 2-Mercapto-5-phenyl-1,3,4-oxadiazole (phozSH) producing the colored mixed ligand complexes with general formula [M(bpdc)(phozSH)(H2O)3] in which the metal ions coordinated to the ligand through O-atoms of carboxyl group in (bpdc) and N-atom of (phozSH) ligand. The ligands and complexes are well identified by using Furrier transform infrared spectroscopy, 1H-NMR, 13C-NMR, Electronic spectroscopy, CHNS analysis, Melting point, conductivity measurement. The Antioxidant activity were screened for all the complexes by the use of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method.

scholarly journals Toxic Effects of Methotrexate on Rat Kidney Recovered by Crocin as a Consequence of Antioxidant Activity and Lipid Peroxidation Prevention

2020 ◽  
Vol 24 (1) ◽  
pp. 39-46
Author(s):  
Cyrus Jalili ◽  
Ali Ghanbari ◽  
Shiva Roshankhah ◽  
Mohammad Reza Salahshoor ◽  
◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Rat Kidney ◽  
Toxic Effects

scholarly journals The effect of bee venom and hyperthermia on the lipoperoxidation and antioxidant activity of the blood of tumor-bearing animals

2021 ◽  
pp. 25-33
Author(s):  
M.A. Shabalin ◽  
◽  
A.V. Deryugina ◽  
V.V. Nazarova ◽  
E.A. Gracheva ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Malignant Tumors ◽  
Combined Treatment ◽  
Bee Venom ◽  
Control Group ◽  
Sod Activity ◽  
Tumor Bearing ◽  
Prolonged Effect ◽  
Control Tumor

Introduction. Nowadays, it has been shown that one of the possible ways to increase the effectiveness of the treatment of malignant tumors is the use of combined treatment methods. Aim. To study the intensity of lipid peroxidation (LPO) and the activity of superoxide dismutase (SOD) in hyperthermia against the background of bee venom in the blood of tumor-bearing rats. Materials and methods. The experimental animals were divided into 5 groups: 1st — intact; 2nd — control (tumor-bearing animals (PS-1 tumor strain) with intraperitoneal (IP) administration of saline solution); 3rd, 4th and 5th groups — tumor-bearing animals with IP administration of 0.5 ml of bee venom against the background of hyperthermia 42, 43 and 44°C respectively. The content of diene and triene conjugates, Schiff bases (SB) and SOD activity in the blood of animals was determined. Results. From the 1st day after the end of the experiment, a statistically significant decrease in triene conjugates was recorded in all experimental groups compared to the control group. SB decreased on the 1st–7th day after the end of the experiment with the action of hyperthermia 42°C and bee venom, on the 7th–28th day — with the action of hyperthermia 43°C and bee venom, and on the 28th day — with the action of bee venom and hyperthermia 44°C, which was accompanied by an increase in SOD activity from the 7th day in all experimental groups compared to the control group. Conclusion. Hyperthermia in combination with the action of bee venom causes a decrease in lipid peroxidation products and an increase in antioxidant activity in the blood of tumor-bearing rats. The most effective action, in our opinion, is the use of bee venom against the background of hyperthermia of 43°C, at which a prolonged effect is recorded both with respect to a decrease in the concentration of SB and an increase in the activity of SOD.

scholarly journals Study of antioxidant activity and anti-cholesterol content on red yeast rice with substrates variation (rice, corn and dried cassava)

2011 ◽  
Vol 9 (2) ◽  
pp. 38-44
Author(s):  
HADI WIYOTO ◽  
M. A.M. ANDRIANI ◽  
NUR HER RIYADI PARNANTO
Keyword(s):  
Antioxidant Activity ◽  
Amylose Content ◽  
Cholesterol Content ◽  
The Other ◽  
Red Yeast Rice ◽  
Red Yeast ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Level Of Significance ◽  
High Amylose

Wiyoto H, Andriani MAM, Parnanto NHR. 2011. Study of antioxidant activity and anti-cholesterol content on red yeast rice with substrates variation (rice, corn and dried cassava). Biofarmasi 9: 38-44. Red yeast rice is one of fermented rice product by Monascus purpureus. Traditionally, the substrate used to produce red yeast rice is rice. Usually, the rice with high amylose content is proper to produce red yeast rice than low amylose. The other substrates that be used to produce red yeast rice are corn and dried cassava. The purposes of this research were to determine the effect of substrates variation (rice, corn and dried cassava) on antioxidant activity and anti-cholesterol content in red yeast rice, and to determine the substrate(s) that produce the highest antioxidant activity and anticholesterol content. The design of this research was a Completely Randomized Design with one factor, i.e. the kind of substrates: rice, corn and dried cassava, with three replications. Then, the data were analyzed with ANOVA at a level of significance α=0.05, and continued with DMRT at the same level. This results showed that the effect of substrates kind to antioxidant activity and anti-cholesterol content on red yeast rice. The rice substrate had higher antioxidant activity and anti-cholesterol content than corn and dried cassava substrates. The antioxidant activity and the anti-cholesterol content on red yeast rice from rice substrate were 45.6100% and 0.026600%, respectively. The antioxidant activity and the anti-cholesterol content on red yeast rice from corn substrate were 44.0500% and 0.022833%, respectively, while the antioxidant activity and the anti-cholesterol content on red yeast rice from dried cassava substrate were 42.8333% and 0.013200%, respectively.

scholarly journals Antioxidant Activity of Water Soaked in Saligrama Stone – A Preliminary Report

2021 ◽  
Vol 21 (1) ◽  
pp. 33
Author(s):  
M. R. Suchitra ◽  
S. Parthasarathy
Keyword(s):  
Antioxidant Activity ◽  
Preliminary Report ◽  
Good Health ◽  
Antioxidant Property ◽  
The Other ◽  
Plain Water ◽  
Dpph Assay ◽  
Beneficial Effects ◽  
Rare Elements

<em>Saligrama</em> otherwise called <em>Shaligram</em> or <em>Salagrama</em> is a fossilized shell stone usually recovered from the banks of the Gandaki river in Nepal. It is supposed to be a sacred symbol of Hindus. Soaking the stone in water for twenty to thirty minutes and partaking the water was considered to give good health. Hence, we mobilized two stones which were authorized as original by experts. The first one was worshipped for more than 5 years and the other one for two years. The water soaked was around 25 ml which was analysed for antioxidant activity by DPPH assay with plain water without soakage as control. The antioxidant property was significantly found in both the stones with more activity in the stone worshipped for more than 5 years. We did not standardize the volume and the duration of soakage to exact values while it was approximated to around 20 – 30 minutes. We suggest more refined studies with estimation of rare elements needs to be performed to establish the beneficial effects of this sacred <em>saligrama</em> stone.

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