scholarly journals The Effects of Acute Hydrogen Sulfide Poisoning on Cytochrome P450 Isoforms Activity in Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Xianqin Wang ◽  
Mengchun Chen ◽  
Xinxin Chen ◽  
Jianshe Ma ◽  
Congcong Wen ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Hydrogen Sulfide ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Upper Respiratory Tract ◽  
Significant Difference ◽  
Cytochrome P450 Isoforms ◽  
Upper Respiratory ◽  
And Control

Hydrogen sulfide (H2S) is the second leading cause of toxin related death (after carbon monoxide) in the workplace. H2S is absorbed by the upper respiratory tract mucosa, and it causes histotoxic hypoxemia and respiratory depression. Cocktail method was used to evaluate the influences of acute H2S poisoning on the activities of cytochrome P450 isoforms CYP2B6, CYP2D6, CYP3A4, CYP1A2, CYP2C19, and CYP2C9, which were reflected by the changes of pharmacokinetic parameters of six specific probe drugs, bupropion, metoprolol, midazolam, phenacetin, omeprazole, and tolbutamide, respectively. The experimental rats were randomly divided into two groups, control group and acute H2S poisoning group (inhaling 300 ppm for 2 h). The mixture of six probes was given to rats by oral administration and the blood samples were obtained at a series of time points through the caudal vein. The concentrations of probe drugs in rat plasma were measured by LC-MS. The results for acute H2S poisoning and control groups were as follows: there was a statistically significant difference in the AUC andCmaxfor bupropion, metoprolol, phenacetin, and tolbutamide, while there was no statistical pharmacokinetic difference for midazolam and omeprazole. Acute H2S poisoning could inhibit the activity of CYP2B6, CYP2D6, CYP1A2, and CYP2C9 in rats.

Comparison of the upper respiratory tract width at the level of the adenoid in patients with normal and abnormal breathing route

2018 ◽  
Vol 14 (2) ◽  
pp. 13-26
Author(s):  
Anna Duda ◽  
Wojciech Stós
Keyword(s):  
Respiratory Tract ◽  
Control Group ◽  
Study Group ◽  
Upper Respiratory Tract ◽  
Control Groups ◽  
Limit Values ◽  
Significant Difference ◽  
Abnormal Breathing ◽  
Upper Respiratory ◽  
And Control

This study assesses the upper respiratory tract width at the level of the adenoid in patients with a normal (nasal) and abnormal (oral or mixed) breathing route. <b>Aim</b>. To compare the upper respiratory tract width in the study group (abnormal breathing route) and control group (normal breathing route) and to determine the cut-off point and mean limit values in order to conduct a differential diagnosis between a habitual and constitutional breathing route. <b>Material and methods</b>. The study included 221 patients treated at the Aquadent-Ortoestetyka Clinic in Kielce. Based on the medical history taken, clinical examination and subjective evaluation of the upper respiratory tract width according to Holmberg 112 patients were enrolled into the study group and 109 patients into the control group. The upper respiratory tract width was measured with two different linear methods: a modified method by Holmberg. and Linder-Aronson, and the Linder-Aronson and Henricson method (AD1-PNS, AD2-PNS).<b> Results</b>. In the study group the mean value of the Holmberg measurement is 4.25 mm, and in the control group – 14.1 mm. Mean values of AD1- PNS and AD2-PNS measurements in the study group are 8.1 mm and 9.5 mm, respectively, and they can be regarded as mean limit values. The 6 mm Holmberg measurement is the cut-off value between the study and control groups, and it should be monitored. In relation to the Holmberg measurement a difference between the study and control groups is 9.85 mm. Regarding AD1-PNS and AD2-PNS measurements a difference between the study and control groups is 11.4 mm and 10.0 mm, respectively.<b> Conclusions</b>. The upper respiratory tract width at the level of the adenoid shows a statistically significant difference between the study and control groups. It may indicate significant narrowing of the upper respiratory tract at the level of the adenoid in the study group or a different type of morphology of the facial skeleton. Limit values obtained in the study allow differentiation of a habitual and constitutional breathing route. <b>(Duda A, Stós W. Comparison of the upper respiratory tract width at the level of the adenoid in patients with normal and abnormal breathing route. Orthod Forum 2018; 14: 106-18)</b>.

scholarly journals Effects of the upper respiratory tract cross-section on the vertical and sagittal dimension and the facial axis according to Ricketts

2018 ◽  
Vol 14 (3) ◽  
pp. 178-191
Author(s):  
Anna Duda ◽  
Wojciech Stós ◽  
Magdalena Wiosna
Keyword(s):  
Respiratory Tract ◽  
Subjective Evaluation ◽  
Threshold Value ◽  
Control Group ◽  
Upper Respiratory Tract ◽  
Facial Skeleton ◽  
Basic Parameters ◽  
Upper Respiratory ◽  
And Control ◽  
Anterior Angle

This study assesses the effects of the upper respiratory tract width at the level of the adenoid in patients with a normal and abnormal breathing route on basic parameters of the morphology of the facial skeleton. <b>Aim.</b> Comparison of skeletal parameters determining vertical (SNL/ML, NL/ML, NL/ML), sagittal (SNA, SNB) dimensions and type of facial rotation (the angle of the facial axis according to Ricketts) in the study and control groups and determination of the threshold value (a cut-off point) of width according to Holmberg, at which there is a deviation from the average values of basic parameters of morphology of the facial skeleton. <b>Material and methods.</b> The study included 221 patients treated at an orthodontic clinic in Kielce. Based on the medical history taken, clinical examination and subjective evaluation of the upper respiratory tract width according to Holmberg 112 patients were enrolled into the study group and 109 patients into the control group. The width of the upper respiratory tract was measured using the modified Holmberg method and parameters assessing the vertical (SNL/ML, NL/ML) and sagittal (SNA, SNB) dimensions, as well as the anterior angle of the Ricketts’ axis were measured using the analysis of lateral teleroentgenograms of the head. <b>Results.</b> All compared skeletal parameters indicated significant statistical differences between the study and control group. These differences were: Ricketts’ angle 6.5°, SNL/ML 8.5°, NL/ML 9.7°, SNA 2.4°, SNB 1.4°. <b>Conclusions.</b> The width of the upper respiratory tract at the level of the adenoid significantly affects values of skeletal parameters evaluating the vertical (SNL/ML, NL/ML) and sagittal (SNA, SNB) dimensions and the type of rotation of the facial axis. There is a correlation between a gradual decrease in the airway dimension according to Holmberg and an increase in the vertical dimension (SNL/ML, NL/ML) and anterior angle of the Ricketts’ facial axis (posterior rotation of the mandible) and a decrease in the sagittal dimension (SNA, SNB).

scholarly journals Dynamic control of local immunity parameters in patients with rhinosinusitis

2018 ◽  
Vol 22 (1) ◽  
pp. 51-54
Author(s):  
Al Hariri Mahmoud Jomaa ◽  
S.I. Semenenko ◽  
A.I. Semenenko ◽  
O.М. Yakubovska
Keyword(s):  
Dynamic Control ◽  
Control Group ◽  
Upper Respiratory Tract ◽  
Local Immunity ◽  
Significant Difference ◽  
Upper Respiratory ◽  
Effective Diagnosis ◽  
Lower Limits ◽  
Baseline Therapy ◽  
Secretory Immunoglobulin

Despite the large number of studies devoted to the problem of diagnosis and treatment of rhinosinusitis remains a discursive issue of effective diagnosis, optimal volume of medical support in such patients. Objective — to determine the degree of violation of the indices of local immunity of the mucous membranes of the upper respiratory tract in patients with rhinosinusitis. 60 patients were examined. All patients were divided into two groups: the first included 30 patients (control group), with rhinosinusitis on baseline therapy, and the second group consisted of 30 patients diagnosed with rhinosinusitis, to which the immunomodulator (inоsine pranobex) was added to the baseline therapy. The drug was administered at a dose of 500 mg. three times a day, within 7 days. In determining the level of sIgA we tested in nasal secretion in patients with rhinosinusitis, it was found that the levels of this secretory immunoglobulin were determined within the lower limits (from 0,72 to 0,98 mg/l) than the standard norms for persons in this age category (1,3–13,3 mg/l) and had no statistically significant difference. In a repeated study of the level sIgA in the subjects treated with baseline therapy and patients who received the immunomodulator (inоsine pranobex) in the basal therapy of rhinosinusitis, the following results were obtained: in the group of patients treated with baseline therapy, an increase in sIgA concentration was observed in 2,1 times, and in the group of patients with the addition of an immunomodulator — 2,6 times, which means a significant improvement in indices of local immunity when adding an immunomodulator to basic therapy. So, in patients with various forms of rhinosinusitis are a low baseline sIgA, indicating insufficient protection of the mucous membrane of the nasopharynx from infectious agents.

Pharmacokinetics of Moxifloxacin in A 5/6th Nephrectomized Rat Model

1970 ◽  
Vol 2 (4) ◽  
pp. 748-755
Author(s):  
Hariprasath Kothandam ◽  
Venkatesh Palaniyappan ◽  
Sudheer Babu Idpuganti ◽  
Umamaheswari Muthusamy
Keyword(s):  
Renal Failure ◽  
Rat Model ◽  
Peak Plasma ◽  
Peak Plasma Concentration ◽  
Area Under The Curve ◽  
Laboratory Animals ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Significant Difference ◽  
And Control

Moxifloxacin (MFLX) is a new 8-methoxyfluoroquinolone derivative with a broad spectrum of antibacterial activity. MFLX at doses of 200 and 400 mg was selected to conduct the pharmacokinetic study and the drug was given orally to control and nephrectomized rats. A 5/6th nephrectomized rat model was used in this study. The drug levels in the plasma were determined using a spectrofluorimetric assay. The pharmacokinetic parameters viz. peak plasma concentration (Cmax) and area under the curve (AUC0-8) of the nephrectomized and control rats were compared. The Cmax for both 200 and 400 mg dose of MFLX in nephrectomized rats showed significant difference(P<0.001) from the control group, which reveals the changes in the Cmax of MFLX in renal failure. The AUC0-8 for both 200 and 400 mg dose of MFLX in nephrectomized rats differ significantly (P<0.001) from sham operated control group, which implies the variation in MFLX availability in altered renal function. The AUC0-8 for 400 mg dose of MFLX in nephrectomized rats differ significantly from 200 mg dose of MFLX in nephrectomized group, which reveals that in higher dose, MFLX shows an abrupt increase in the drug availability in renal failure. It is conclude that preclinical drug monitoring of moxifloxacin in laboratory animals can be performed by using 5/6th nephrectomized rat models for determining the dose of MFLX for kidney failured patients. Various pharmacokinetic parameters determined differed in nephrectomised rats when compared to the control.

Altered pharmacokinetics and pharmacodynamics of repaglinide by ritonavir in rats with healthy, diabetic and impaired hepatic function

2016 ◽  
Vol 31 (2) ◽  
Author(s):  
Thirumaleswara Goud ◽  
Srinivas Maddi ◽  
Devanna Nayakanti ◽  
Rajendra Prasad Thatipamula
Keyword(s):  
Cytochrome P450 ◽  
Plasma Concentration ◽  
Organic Anion ◽  
Hepatic Function ◽  
The Body ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Impaired Hepatic Function ◽  
Significant Difference

AbstractRitonavir is an antiretroviral drug to treat HIV AIDS and inhibits cytochrome P450 3A4. To treat diabetes mellitus in HIV, repaglinide is coadministered with ritonavir in the clinic. Multiple cytochrome P450 (CYP) isoforms are involved in the metabolism of repaglinide like CYP2C8 and CYP 3A4. In order to predict and understand drug-drug interactions of these two drugs, the pharmacokinetics and pharmacodynamics (PK/PD) of repaglinide and ritonavir were studied in normal, diabetic and hepatic impaired rats. The purpose of the study was to assess the influence of ritonavir on the PK/PD of repaglinide in rats with normal, diabetic and impaired hepatic function.Human oral therapeutic doses of ritonavir and repaglinide were extrapolated to rats based on the body surface area. Ritonavir (20 mg/kg, p.o.), alone and along with repaglinide (0.5 mg/kg, p.o.), was given to normal, diabetic and hepatic impaired rats, and the PK/PD were studied.The pharmacokinetic parameters like peak plasma concentration (Cmax), area under the plasma concentration time profile (AUC) and elimination half life of repaglinide were significantly (p<0.0001) increased when compared to repaglinide control rats. The repaglinide clearance (CL) was significantly (p<0.0001) decreased in the presence of ritonavir treatment. In the presence of ritonavir, repaglinide hypoglycemic activity was increased significantly (p<0.0005) when compared with repaglinide control group.The significant difference in the PK/PD changes have been due to the increased plasma exposure and decreased total body clearance of repaglinide, which may be due to the inhibition of the CYP P450 metabolic system and organic anion-transporting polypeptide transporter by ritonavir.

scholarly journals Drug Interaction Effect of Ketoprofen and Dexamethasone With Thiopental: A Pharmacokinetics and Anesthetics Study in Male Dogs

2021 ◽  
Author(s):  
Mahdieh Raeeszadeh ◽  
Mohammad Pouya Ghaffari ◽  
Abolfazl Akbari
Keyword(s):  
Hplc Method ◽  
Distribution Volume ◽  
Group Differences ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Healthy Male ◽  
Control Groups ◽  
Significant Difference ◽  
Time Parameters ◽  
And Control

Abstract There is a growing concern about drug interactions on the pharmacokinetic parameters of anesthetics. The aim of this study was to evaluate the pharmacokinetic effects of thiopental anesthesia under the administration of ketoprofen and dexamethasone in the male dogs. 21 adult healthy male stray dogs weighing 21-23 kg were randomly divided into three groups. The control group received normal saline (0.2 ml/kg) 5 minutes before intravenous administration of thiopental (17 mg/kg), the T1 group received ketoprofen (2.2 mg/kg) 5 minutes before thiopental, and the T2 group received dexamethasone (0.2 mg/kg) 5 minutes before thiopental. After anesthesia, clinical parameters of anesthesia, heart rate, respiration rate and electrocardiography were measured. Serum sample was also used to assay thiopental concentration using HPLC method, and then thiopental pharmacokinetic parameters were calculated. Changes in rate of heart and respiration were significant intra-group differences 5 and 10 minutes after anesthesia.In addition, in the ketoprofen group, premature atrial contractions (PACs) were seen after induction of anesthesia with thiopental. Recovery time parameters showed a significant difference between T1 and control groups (P<0.05). Elimination and half-life of thiopental in the T1 group compared to the control and T2 groups showed a significant decrease and increase, respectively. In addition, the distribution volume of thiopental in T1 showed a significant increase compared to other groups. However, thiopental clearance in T1 and T2 groups did not show a significant decrease compared to control (P> 0.05). It can be concluded that dexamethasone can be used as an anti-inflammatory drug combination with thiopental in comparison with ketoprofen.

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim
Keyword(s):  
Allergic Rhinitis ◽  
Side Effects ◽  
Coconut Oil ◽  
Test Group ◽  
Nasal Symptom ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

THE EFFECT OF THE ATTITUDE TOWARD BREASTFEEDING CONCEPT BASED COMIC ON THE ADOLESCENCE'S ATTITUDE TOWARD EXCLUSIVE BREASTFEEDING

2017 ◽  
Vol 1 (2) ◽  
pp. 138
Author(s):  
Mekar Dwi Anggraeni ◽  
Lutfatul Latifah ◽  
Aprilia Kartikasari ◽  
Ima Rismawati
Keyword(s):  
Exclusive Breastfeeding ◽  
Nuclear Family ◽  
T Test ◽  
Breastfeeding Duration ◽  
Control Group ◽  
Marriage Age ◽  
First Marriage ◽  
Significant Difference ◽  
Post Test ◽  
And Control

Background and purpose: The earlier development of the attitude toward exclusive breastfeeding produces the longer exclusive breastfeeding duration. Considering the first marriage age among Indonesian, the attitude toward exclusive breastfeeding should be developed at the adolescence age. The purpose of this study was to examine the effect of the attitude toward breastfeeding concept based comic on the adolescent's attitude toward exclusive breastfeeding. Method: This was a quasy experimental posttest only with control group study. The respondents were provided an comic. The respondent's attitude toward exclusive breastfeeding was measured using The Breastfeeding Attitude Questionnaire. Data were analyzed using independent and dependent t test. Results: The majority of respondents were aged 17 years old in both intervention (70%) and control grup (63%), first child in both intervention (23,3%) and control grup (26,7%), and had a nuclear family in both intervention (80%) and control grup (90%). The independent t test showed that there was a significant difference between post-test scores among the intervention and control groups (t = 5,602, p < 0,01). Conclusion and recommendation: Nurses may use the Attitude Toward Breastfeeding based comic to increase the Adolescence's attitude toward breastfeeding.Keywords: Comic, Attitudes Toward Exclusive Breastfeeding, Adolescence

Impact of intrawound vancomycin powder on prevention of surgical site infection after posterior spinal surgery

2021 ◽  
pp. 1-9
Author(s):  
Hiroki Ushirozako ◽  
Tomohiko Hasegawa ◽  
Yu Yamato ◽  
Go Yoshida ◽  
Tatsuya Yasuda ◽  
...  
Keyword(s):  
Propensity Score ◽  
Spinal Surgery ◽  
Control Group ◽  
Control Groups ◽  
Propensity Score Model ◽  
Significant Difference ◽  
Surgical Data ◽  
Vancomycin Powder ◽  
Vancomycin Group ◽  
And Control

OBJECTIVESurgical site infection (SSI) after posterior spinal surgery is one of the severe complications that may occur despite administration of prophylactic antibiotics and the use of intraoperative aseptic precautions. The use of intrawound vancomycin powder for SSI prevention is still controversial, with a lack of high-quality and large-scale studies. The purpose of this retrospective study using a propensity score–matched analysis was to clarify whether intrawound vancomycin powder prevents SSI occurrence after spinal surgery.METHODSThe authors analyzed 1261 adult patients who underwent posterior spinal surgery between 2010 and 2018 (mean age 62.3 years; 506 men, 755 women; follow-up period at least 1 year). Baseline and surgical data were assessed. After a preliminary analysis, a propensity score model was established with adjustments for age, sex, type of disease, and previously reported risk factors for SSI. The SSI rates were compared between patients with intrawound vancomycin powder treatment (vancomycin group) and those without (control group).RESULTSIn a preliminary analysis of 1261 unmatched patients (623 patients in the vancomycin group and 638 patients in the control group), there were significant differences between the groups in age (p = 0.041), body mass index (p = 0.013), American Society of Anesthesiologists classification (p < 0.001), malnutrition (p = 0.001), revision status (p < 0.001), use of steroids (p = 0.019), use of anticoagulation (p = 0.033), length of surgery (p = 0.003), estimated blood loss (p < 0.001), and use of instrumentation (p < 0.001). There was no significant difference in SSI rates between the vancomycin and control groups (21 SSIs [3.4%] vs 33 SSIs [5.2%]; OR 0.640, 95% CI 0.368–1.111; p = 0.114). Using a one-to-one propensity score–matched analysis, 444 pairs of patients from the vancomycin and control groups were selected. There was no significant difference in the baseline and surgical data, except for height (p = 0.046), between both groups. The C-statistic for the propensity score model was 0.702. In the score-matched analysis, 12 (2.7%) and 24 (5.4%) patients in the vancomycin and control groups, respectively, developed SSIs (OR 0.486, 95% CI 0.243–0.972; p = 0.041). There were no systemic complications related to the use of vancomycin.CONCLUSIONSThe current study showed that intrawound vancomycin powder was useful in reducing the risk of SSI after posterior spinal surgery by half, without adverse events. Intrawound vancomycin powder use is a safe and effective procedure for SSI prevention.

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