scholarly journals Evaluation of Gellan Gum Film Containing Virgin Coconut Oil for Transparent Dressing Materials

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Nur Arifah Ismail ◽  
Siti Fatimah Mohamad ◽  
Maizatul Akma Ibrahim ◽  
Khairul Anuar Mat Amin
Keyword(s):  
Bacterial Resistance ◽  
Composite Films ◽  
Coconut Oil ◽  
Gellan Gum ◽  
Material Surface ◽  
Human Skin Fibroblast ◽  
Virgin Coconut Oil ◽  
Swelling Properties ◽  
Dressing Materials

We examined the potential of virgin coconut oil (VCO) incorporated in gellan gum (GG) films as a dressing material. Pure GG film is extremely brittle and inclusion of 0.3% (w/w) VCO in the GG film (GG-VCO3) improved the toughness (T≈0.67±0.33 J g−1) of the composite films. Swelling properties and water vapor transmission rates of GG-VCO composite films decreased, whereas thermal behavior values increased upon the addition of higher concentrations of VCO. Cell studies exhibit that the VCO is noncytotoxic to human skin fibroblast cells (CRL2522) with limited cell growth observed on GG-VCO3 films at 1,650 cells/well after incubation for 72 h which could be due to hydrophobic influence of the material surface. The qualitative and in vitro quantitative antibacterial results revealed that VCO does not possess strong bacterial resistance against all four tested bacteria, that is, two Gram-positive (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram-negative bacteria (Pseudomonas aeruginosa and Proteus mirabilis).

scholarly journals The Role of Coconut Oil to Increase Expression of MMP-9, PDGF-BB, and TGF-β1 in NIH-3T3 Cell Line

2019 ◽  
Vol 7 (22) ◽  
pp. 3733-3736
Author(s):  
Dian Ika Perbina Meliala ◽  
Jansen Silalahi ◽  
Yuandani Yuandani ◽  
Linda Margata ◽  
Denny Satria
Keyword(s):  
Healing Process ◽  
Coconut Oil ◽  
Wound Healing Process ◽  
3T3 Cells ◽  
Virgin Coconut Oil ◽  
Nih3t3 Cells ◽  
Nih 3T3 ◽  
Tgf Β1 ◽  
Nih 3T3 Cells

AIM: The objective of the study was to evaluate protein expression in NIH 3T3 cells that are treated with virgin coconut oil (VCO) and hydrolysed of virgin coconut oil (HVCO) in vitro. METHODS: Coconut oil used in this study was virgin coconut oil (VCO) and VCO hydrolysed by Rhizomucor miehei (HVCO). NIH 3T3 cells (5x105 cells/well) were seeded in nine wells and incubated for overnight, then divided into three groups. Each group consisted of three wells. Group one without treatment, group two added VCO, and group three added HVCO and then incubated for overnight. One well in each group was added MMP-9, PDGF-BB, and TGF-β1 and incubated one hour. Finally, expressions of MMP-9, PDGF-BB, and TGF-β1 were detected using immunocytochemistry method. RESULTS: The results of the study showed that VCO and HVCO increased protein expressions of MMP-9, PDGF-BB, and TGF-β1. Percentage of MMP-9 expressions treated by VCO increased from 2.89 ± 0.07 to 28.16 ± 0.34, PDGF-BB from 28.11 ± 0.13 to 48.53 ± 0.49, and TGF-β1 from 4.19 ± 0.08 to 18.41 ± 0.54. Percentage of MMP-9 expressions treated by HVCO increased from 2.89 ± 0.07 to 55.40 ± 0.94, PDGF-BB from 28.11 ± 0.13 to 61.65 ± 0.42, and TGF-β1 from 4.19 ± 0.08 to 36.35 ± 0.67. CONCLUSION: VCO and HVCO increase the expression of MMP-9, PDGF-BB, dan TGF-β1 in NIH3T3 cells and therefore, coconut oil active in the wound healing process. HVCO is more than active than VCO.

Virgin coconut oil complements with its polyphenol components mitigate sodium fluoride toxicity in vitro and in vivo

2021 ◽  
pp. 1-7
Author(s):  
Soorya Parathodi Illam ◽  
Sruthi Panniyan Kandiyil ◽  
Arunaksharan Narayanankutty ◽  
Soumya Valappan Veetil ◽  
Thekkekara Devassy Babu ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Coconut Oil ◽  
Fluoride Toxicity ◽  
Virgin Coconut Oil ◽  
Toxicity In Vitro

scholarly journals In vitro anti-inflammatory and skin protective properties of Virgin coconut oil

2019 ◽  
Vol 9 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Sandeep R. Varma ◽  
Thiyagarajan O. Sivaprakasam ◽  
Ilavarasu Arumugam ◽  
N. Dilip ◽  
M. Raghuraman ◽  
...  
Keyword(s):  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
Protective Properties ◽  
Anti Inflammatory

Should virgin coconut oil be used in the adjunctive treatment of COVID-19?

2020 ◽  
Vol 54 ◽  
Author(s):  
Carol Stephanie C. Tan-Lim ◽  
Corinna Victoria Martinez
Keyword(s):  
Lauric Acid ◽  
Animal Studies ◽  
Coconut Oil ◽  
The Philippines ◽  
Adjunctive Treatment ◽  
Virgin Coconut Oil ◽  
Ssrna Virus ◽  
Immunodeficiency Virus ◽  
Pharmacologically Active

KEY FINDINGS There is currently no evidence to support the use of virgin coconut oil in the adjunctive treatment of COVID-19. • Virgin coconut oil is naturally extracted from fresh coconut kernel and is rich in medium chain triglycerides, with lauric acid as the predominant fatty acid.• Virgin coconut oil is currently explored as an adjunctive treatment for patients with COVID-19 due to its antiviral and immunomodulatory properties.• In vitro studies show that lauric acid or its derivative exert inhibitory activities against viruses with similar structure to coronavirus (enveloped ssRNA virus) such as Junin virus, vesicular stomatitis virus, human immunodeficiency virus type 1 (HIV-1), and Semliki Forest virus.• Animal studies demonstrate antiviral activity of monolaurin, the pharmacologically active metabolite of lauric acid, on avian influenza virus and Simean immunodeficiency virus, which are both enveloped ssRNA viruses.• Clinical trials among patients with HIV report that virgin coconut oil can increase CD4+ T lymphocyte counts and reduce viral load.• In vitro and animal studies demonstrate anti-inflammatory properties of virgin coconut oil.• At present, there are no studies that investigate the effectiveness of virgin coconut oil in the adjunctive treatment of COVID-19 infection.• There is currently one ongoing clinical trial in the Philippines evaluating the use of virgin coconut oil in the adjunctive treatment of COVID-19.• Nausea, vomiting, mild diarrhea, and abdominal pain have been reported, but no serious adverse events have been identified with the use of virgin coconut oil.• To date, there are no guidelines that mention virgin coconut oil as an option for the adjunctive treatment of COVID-19.

Injectable Surfactant Included-Virgin Coconut Oil with and without Ibuprofen

2012 ◽  
Vol 581-582 ◽  
pp. 108-111 ◽  
Author(s):  
Sumuntana Anuchatkidjaroen ◽  
Thawatchai Phaechamud
Keyword(s):  
Drug Release ◽  
Physical Properties ◽  
Cloud Point ◽  
Pour Point ◽  
Polarized Light ◽  
Coconut Oil ◽  
Temperature Sensitive ◽  
Virgin Coconut Oil ◽  
Wax Deposition

In the tropical countries, virgin coconut oil (VCO) has been abundantly utilized as traditional medicine and cosmetic, but its major problem is temperature sensitive. This oil changes into some wax-like at cool environment. The purpose of this study is to decrease wax deposition of this oil by investigate the effect of surfactants on the physical properties and drug release characteristic. Ibuprofen (IB), which can soluble in VCO, was used as a model drug. Viscosity, pour point, cloud point and polarized light microscope examinations were conducted to characterize the change of VCO physical properties. In vitro drug release experiment was performed using dialysis method at 50 rpm and 37°C in phosphate buffer pH 7.4. The addition of surfactants in VCO increased the efficiency for measuring the viscosity at lower temperature. Result from viscosity measurement indicated that Solutol® HS15 (ST) was the most suitable for choosing as representative of the surfactants. Both pour point and cloud point could not reduce by ST because the crystals size of VCO with and without ST was not different. There was no difference of viscosity of each formula during the release experiments (37°C), therefore the release rate of drug from VCO containing or without ST was not different. These indicated that the surfactants and ibuprofen affected the physical properties but did not affect the release of this investigated VCO.

scholarly journals In vitro Evaluation of Antifungal Activity of Virgin Coconut oil and White Palm Kernel Oil on Candida Species-Experimental Study

2019 ◽  
pp. 1-9
Author(s):  
Hortense Gonsu Kamga ◽  
Sarah Riwom Essama ◽  
Yves Le Grand Napa Tchuedji ◽  
Maurice Boda ◽  
Stève Henri Voundi Olugu ◽  
...  
Keyword(s):  
Experimental Study ◽  
Candida Albicans ◽  
Candida Parapsilosis ◽  
Palm Kernel ◽  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
Palm Kernel Oil ◽  
Kernel Oil ◽  
Maximum Inhibition

Aims: The aim of this study is to evaluate in vitro the antifungal activity of virgin coconut oil and the white palm kernel oil on the growth of six species of the genus Candida. Study Design: This study is an experimental study. Place and Duration of the Study: Department of Microbiology, Laboratory of Microbiology of the University of Yaoundé I. Laboratory of Bacteriology, Yaoundé University teaching Hospital between April to September 2017. Methodology: We included six species of Candida. The method of diffusion discs in agar medium was used for Sensitivity tests and macro-dilution in liquid medium was used for dilution. Results: Majority of the species tested were resistant to conventional antifungals used. Candida hoemulonii was the most sensitive species to virgin coconut oil with percentages of inhibitions higher than 50 % when the concentration of oil was only 3.125 mg/mL and a maximum inhibition percentage of 90.10 % when the concentration of oil was 100 mg/mL. Candida albicans, was the least susceptible species to virgin coconut oil with a maximum inhibition percentage of 59.85 % when the concentration of oil was 100 mg/mL.  Candida lipolytica was the most sensitive specie to white palm kernel oil with a maximum inhibition percentage of 90,26 % when the concentration of oil was 100 mg/mL and Candida parapsilosis was the least susceptible species to white palm kernel oil with a maximum inhibition percentage of 52,69 % at the same concentration. In addition, the white palm kernel oil which was more active (P˂0.05) than virgin coconut oil was introduced in the Sabouraud broth and the MIC and MFC obtained with Fluconazole was lower on Candida albicans and Candida parapsilosis compared to activity of Fluconazole without palm kernel oil in the medium. Nystatin showed also lower MIC and MFC values on Candida parapsilosis when palm kernel oil was present in the culture medium. Conclusion: These results prove that these oils can be used to develop antifungals drugs.

scholarly journals KINETIKA PERMEASI KLOTRIMAZOL DARI MATRIKS BASIS KRIM YANG MENGANDUNG VIRGIN COCONUT OIL (VCO)

2015 ◽  
Vol 2 (1) ◽  
pp. 14 ◽  
Author(s):  
Henny Lucida ◽  
Patihul Husni ◽  
Vinny Hosiana
Keyword(s):  
Rate Constant ◽  
In Vitro Release ◽  
Coconut Oil ◽  
Release Profile ◽  
Diffusion Cell ◽  
Virgin Coconut Oil ◽  
The Matrix ◽  
Base Matrix ◽  
Vitro Release

 ABSTRACT A kinetic study on the release of clotrimazole from a VCO containing creambase has been undertaken. The in-vitro release of the drug was studied by using a modification diffusion cell apparatus. Four formulations of clotrimazole cream were prepared, each contained either VCO or paraffin liquidum in the cream base. The amount of clotrimazole release were determined by UV spectrophotometer. Results showed that release of clotrimazole from all formulations followed Higuchi kinetics, the release rate constant from F1 (containing VCO) was significantly different than that from F1’ (containing paraffin liquidum) (p < 0.05). The rate constant of clotrimazole from F2 (containing VCO); F2’ (containing paraffin liquidum ) and F1 were not significantly different. Virgin coconut oil (VCO) was a potential cream base matrix regarding the release profile of clotrimazole from the matrix. Keywords : clotrimazol, VCO, permeation kinetic

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