Virgin coconut oil complements with its polyphenol components mitigate sodium fluoride toxicity in vitro and in vivo

2021 ◽  
pp. 1-7
Author(s):  
Soorya Parathodi Illam ◽  
Sruthi Panniyan Kandiyil ◽  
Arunaksharan Narayanankutty ◽  
Soumya Valappan Veetil ◽  
Thekkekara Devassy Babu ◽  
...  
Keyword(s):  
Sodium Fluoride ◽  
Coconut Oil ◽  
Fluoride Toxicity ◽  
Virgin Coconut Oil ◽  
Toxicity In Vitro

Derivatives of Deoxypodophyllotoxin Induce Apoptosis Through Bcl-2/Bax Proteins Expression

2020 ◽  
Vol 20 ◽  
Author(s):  
Ya-Nan Li ◽  
Ni Ning ◽  
Lei Song ◽  
Yun Geng ◽  
Jun-Ting Fan ◽  
...  
Keyword(s):  
Annexin V ◽  
Mtt Method ◽  
Anthriscus Sylvestris ◽  
Anti Tumor Activity ◽  
Derivatives Of ◽  
Toxicity In Vitro ◽  
Ht 29 ◽  
Mcf 7

Background: Deoxypodophyllotoxin, isolated from theTraditional Chinese Medicine Anthriscus sylvestris, is well-known because of its significant antitumor activity with strong toxicity in vitro and in vivo. Objective: In this article, we synthesized a series of deoxypodophyllotoxin derivatives, and evaluated their antitumor effectiveness.Methods:The anti tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC. Results: The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29 andMG-63 using Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined by the ELISA kit. Conclusion: The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing Bcl-2/Bax.

The Toxic Effects of Two Dental Materials on Human Buccal Epithelium In Vitro and Monkey Buccal Epithelium In Vivo

1987 ◽  
Vol 14 (3) ◽  
pp. 166-167
Author(s):  
D. Arenholt-Bindslev ◽  
P. Hørsted-Bindslev ◽  
H.P. Philipsen
Keyword(s):  
Dental Materials ◽  
Microscopical Examination ◽  
Buccal Epithelium ◽  
Cytotoxic Effects ◽  
Buccal Epithelial Cells ◽  
Toxicity In Vitro ◽  
Toxic Reactions ◽  
Light Microscopical Examination

The aim of the present study was to compare the toxicity in vitro with the toxicity in vivo of two commercial chemicals marketed for use in the oral cavity (GLUMA BondR and 3M Etching LiquidR). Confluent cultures of human buccal epithelial cells were exposed to graded concentrations of GLUMA Bond or 3M Etching Liquid for 5 minutes. The cytotoxic effects induced by this treatment were observed (cytomorphology, proliferation rate). In vivo, monkey buccal epithelium was exposed to GLUMA Bond or 3M Etching Liquid for 5 minutes. Biopsies were taken after 24 hours, and the buccal epithelium processed for light microscopical examination. In both models, the toxic reactions to GLUMA Bond were far more extensive than those caused by 3M Etching Liquid.

Antimicrobial and antibiofilm activities of meropenem loaded-mesoporous silica nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

2021 ◽  
pp. 088532822110038
Author(s):  
Mohammad Yousef Memar ◽  
Mina Yekani ◽  
Hadi Ghanbari ◽  
Edris Nabizadeh ◽  
Sepideh Zununi Vahed ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Carbapenem Resistant ◽  
Toxicity In Vitro ◽  
Different Levels

The aims of the present study were the determination of antimicrobial and antibiofilm effects of meropenem-loaded mesoporous silica nanoparticles (MSNs) on carbapenem resistant Pseudomonas aeruginosa ( P. aeruginosa) and cytotoxicity properties in vitro. The meropenem-loaded MSNs had shown antibacterial and biofilm inhibitory activities on all isolates at different levels lower than MICs and BICs of meropenem. The viability of HC-04 cells treated with serial concentrations as MICs and BICs of meropenem-loaded MSNs was 92–100%. According to the obtained results, meropenem-loaded MSNs display the significant antibacterial and antibiofilm effects against carbapenem resistant and biofilm forming P. aeruginosa and low cell toxicity in vitro. Then, the prepared system can be an appropriate option for the delivery of carbapenem for further evaluation in vivo assays.

scholarly journals Fabrication of novel vesicles of triptolide for antirheumatoid activity with reduced toxicity in vitro and in vivo

2016 ◽  
pp. 2663 ◽  
Author(s):  
Qianqian Liang ◽  
Li Zhang ◽  
Tengteng Wang ◽  
Qiang Li ◽  
Jing Huang ◽  
...  
Keyword(s):  
Reduced Toxicity ◽  
Toxicity In Vitro

The Assessment of Bioreductive Drug Toxicity in vitro and in Experimental Tumours in vivo

1990 ◽  
pp. 203-212 ◽  
Author(s):  
I. J. Stratford ◽  
G. E. Adams ◽  
J. C. M. Bremner ◽  
S. Cole ◽  
Li Ding ◽  
...  
Keyword(s):  
Drug Toxicity ◽  
Toxicity In Vitro

scholarly journals Spike Protein Targeting "Nano-Glue" that Captures and Promotes SARS-CoV-2 Elimination

2021 ◽  
Author(s):  
Guofang Zhang ◽  
Yalin Cong ◽  
Guoli Cao ◽  
Liang Li ◽  
Peng Yu ◽  
...  
Keyword(s):  
Protein Targeting ◽  
Binding Capacity ◽  
Spike Protein ◽  
Therapeutic Drug ◽  
Receptor Binding Domain ◽  
Airway Epithelial ◽  
Β Sheet ◽  
Toxicity In Vitro

The global emergency caused by the SARS-CoV-2 pandemics can only be solved with adequate preventive and therapeutic strategies, both currently missing. The electropositive Receptor Binding Domain (RBD) of SARS-CoV-2 spike protein with abundant β-sheet structure serves as target for COVID-19 therapeutic drug design. Here, we discovered that ultrathin 2D CuInP2S6 (CIPS) nanosheets as a new agent against SARS-CoV-2 infection, which also able to promote viral host elimination. CIPS exhibits extremely high and selective binding capacity with the RBD of SARS-CoV-2 spike protein, with consequent inhibition of virus entry and infection in ACE2-bearing cells and human airway epithelial organoids. CIPS displays nano-viscous properties in selectively binding with spike protein (KD < 1 pM) with negligible toxicity in vitro and in vivo. Further, the CIPS-bound SARS-CoV-2 was quickly phagocytosed and eliminated by macrophages, suggesting CIPS could be successfully used to capture and facilitate the virus host elimination with possibility of triggering anti-viral immunization. Thus, we propose CIPS as a promising nanodrug for future safe and effective anti-SARS-CoV-2 therapy, as well as for use as disinfection agent and surface coating material to constrain the SARS-CoV-2 spreading.

scholarly journals The Role of Coconut Oil to Increase Expression of MMP-9, PDGF-BB, and TGF-β1 in NIH-3T3 Cell Line

2019 ◽  
Vol 7 (22) ◽  
pp. 3733-3736
Author(s):  
Dian Ika Perbina Meliala ◽  
Jansen Silalahi ◽  
Yuandani Yuandani ◽  
Linda Margata ◽  
Denny Satria
Keyword(s):  
Healing Process ◽  
Coconut Oil ◽  
Wound Healing Process ◽  
3T3 Cells ◽  
Virgin Coconut Oil ◽  
Nih3t3 Cells ◽  
Nih 3T3 ◽  
Tgf Β1 ◽  
Nih 3T3 Cells

AIM: The objective of the study was to evaluate protein expression in NIH 3T3 cells that are treated with virgin coconut oil (VCO) and hydrolysed of virgin coconut oil (HVCO) in vitro. METHODS: Coconut oil used in this study was virgin coconut oil (VCO) and VCO hydrolysed by Rhizomucor miehei (HVCO). NIH 3T3 cells (5x105 cells/well) were seeded in nine wells and incubated for overnight, then divided into three groups. Each group consisted of three wells. Group one without treatment, group two added VCO, and group three added HVCO and then incubated for overnight. One well in each group was added MMP-9, PDGF-BB, and TGF-β1 and incubated one hour. Finally, expressions of MMP-9, PDGF-BB, and TGF-β1 were detected using immunocytochemistry method. RESULTS: The results of the study showed that VCO and HVCO increased protein expressions of MMP-9, PDGF-BB, and TGF-β1. Percentage of MMP-9 expressions treated by VCO increased from 2.89 ± 0.07 to 28.16 ± 0.34, PDGF-BB from 28.11 ± 0.13 to 48.53 ± 0.49, and TGF-β1 from 4.19 ± 0.08 to 18.41 ± 0.54. Percentage of MMP-9 expressions treated by HVCO increased from 2.89 ± 0.07 to 55.40 ± 0.94, PDGF-BB from 28.11 ± 0.13 to 61.65 ± 0.42, and TGF-β1 from 4.19 ± 0.08 to 36.35 ± 0.67. CONCLUSION: VCO and HVCO increase the expression of MMP-9, PDGF-BB, dan TGF-β1 in NIH3T3 cells and therefore, coconut oil active in the wound healing process. HVCO is more than active than VCO.

scholarly journals BODY SCRUB VIRGIN COCONUT OIL, COFFEE GROUNDS AND CARBON ACTIVE COCONUT SHELL AS A MOISTURIZER AND SKIN BRIGHTENING

2021 ◽  
Vol 17 (1) ◽  
pp. 62-67
Author(s):  
Putri Desi Eka ◽  
Djamil Ratna ◽  
Faizatun Faizatun
Keyword(s):  
Fatty Acids ◽  
Activated Carbon ◽  
Coffea Arabica ◽  
Coconut Oil ◽  
Virgin Coconut Oil ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Coffee Grounds ◽  
Black Color

Relevance. Virgin Coconut Oil (VCO) contains a lot of medium chain fatty acids, combined with coffee grounds (Coffea Arabica Linn.) and activated carbon (Activated carbon coconut Nucifera L) into a preparation that can moisturize and brighten the skin. Objective. The purpose of this study was to make cosmetic cleansing preparations containing three natural ingredients. Methods. This study evaluates the organoleptic body scrub preparations, homogeneity, dispensability, and pH. The ingredients were tested using in-vivo and clinical irritation along with the effectiveness of the preparation (moisture and brightness). Results. Organoleptic testing and homogeneity were confirmed to have dark black color, the distinctive smell of coffee, homogeneity, spreadability in the range of 4 cm, and the pH was at a safe pH for the skin so that irritation results also did not show edema and erythema both in-vivo divided into 3 groups (n = 3) and clinically (n = 30). In addition, this body scrub provides moisture and brightness to the skin for 2 months of use, p <0.05. Conclusions. VCO body scrub preparations, coffee grounds, and activated carbon are preparations that have the potential to be cosmetic cleansers.

scholarly journals Protective Effects of Thiol Compounds on Chromate-Induced Toxicity In Vitro and In Vivo

1994 ◽  
Vol 102 ◽  
pp. 247 ◽  
Author(s):  
Nobuyuki Susa ◽  
Shunji Ueno ◽  
Yoshinori Furukawa
Keyword(s):  
Protective Effects ◽  
Thiol Compounds ◽  
Toxicity In Vitro
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