scholarly journals Componential Profile and Amylase Inhibiting Activity of Phenolic Compounds fromCalendula officinalisL. Leaves

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Daniil N. Olennikov ◽  
Nina I. Kashchenko
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acid ◽  
Soluble Fraction ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Inhibiting Activity ◽  
Inhibitory Effects ◽  
Calendula Officinalis ◽  
Caffeoylquinic Acid ◽  
Main Components

An ethanolic extract and its ethyl acetate-soluble fraction from leaves ofCalendula officinalisL. (Asteraceae) were found to show an inhibitory effect on amylase. From the crude extract fractions, one new phenolic acid glucoside, 6′-O-vanilloyl-β-D-glucopyranose, was isolated, together with twenty-four known compounds including five phenolic acid glucosides, five phenylpropanoids, five coumarins, and nine flavonoids. Their structures were elucidated based on chemical and spectral data. The main components, isoquercitrin, isorhamnetin-3-O-β-D-glucopyranoside, 3,5-di-O-caffeoylquinic acid, and quercetin-3-O-(6′′-acetyl)-β-D-glucopyranoside, exhibited potent inhibitory effects on amylase.

scholarly journals Methylkarranjic acid and Pongamol from Derris indica Seeds and their Antibacterial Activity

1970 ◽  
Vol 6 (1) ◽  
pp. 9-13 ◽  
Author(s):  
M Abdullahil Baki ◽  
Golam Sadik ◽  
KAM Shahadat Hossain Mondal ◽  
M Ashilk Mosaddik ◽  
M Mukhlesur Rahman
Keyword(s):  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Soluble Fraction ◽  
Ethanolic Extract ◽  
2D Nmr ◽  
Shigella Dysenteriae ◽  
E Coli ◽  
Nmr Data ◽  
Test Organisms ◽  
Derris Indica

Two phenolic compounds, methylkarranjic (1) acid and pongamol (2), were isolated from the petroleum ether soluble fraction of an ethanolic extract of Derris indica seeds. The structures of these compounds were confirmed by LC-MS and a series of 1D and 2D NMR data. The solvent solvent partionates of the ethanolic extract and compounds (1 and 2) exhibited moderate antibacterial activity against several test organisms. The minimum inhibitory concentrations (MICs) of 1 and 2 were found to be in the range of 32-128 μg/ml against Bacillus megatorium, Streptococcus β-haemolyticus, Shigella dysenteriae and E. coli. Key words: Derris indica; Leguminosae; Methylkarranjic acid; Pongamol; Antibacterial activity Dhaka Univ. J. Pharm. Sci. 6(1): 9-13, 2007 (June) The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals A Bio-Guided Fractionation to Assess the Inhibitory Activity ofCalendula officinalisL. on the NF-κB Driven Transcription in Human Gastric Epithelial Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Elisa Colombo ◽  
Enrico Sangiovanni ◽  
Michele D’Ambrosio ◽  
Enrica Bosisio ◽  
Alexandru Ciocarlan ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Calendula Officinalis ◽  
Active Compounds ◽  
Significant Inhibitory Effect ◽  
Dependent Inhibition ◽  
Anti Inflammatory ◽  
Main Components ◽  
Triterpene Esters

Calendula officinalisL. has been largely known for its topical anti-inflammatory properties; however, there are no experimental evidences about its antiphlogistic effect at the gastric level. To investigate whether marigold might exert an activity against gastric inflammation, a CH2Cl2extract obtained fromC. officinalisflowers was evaluatedin vitroon the NF-κB pathway. The lipophilic extract demonstrated a significant inhibitory effect on the NF-κB driven transcription. The identification of active compounds was conducted by a bio-guided fractionation of the extract that afforded 16 fractions. Fraction J exhibited a concentration-dependent inhibitory activity on the NF-κB driven transcription and significantly contributed to the antiphlogistic effect showed by CH2Cl2extract. The main components of fraction J were loliolide and the fucoside acetates ofβ-eudesmol and viridiflorol. HPLC analysis of fractions D and E led to the identification and isolation of triterpene esters that showed a concentration-dependent inhibition of the NF-κB driven transcription, with faradiol-3-myristate and the corresponding aglycone being the most active compounds. The present study provides some experimental evidences thatCalendula officinalisL. may exert an anti-inflammatory activity on the gastric district by the inhibition of the NF-κB system, identifying the compounds responsible, at least in part, for the observed effect.

scholarly journals Efficacy of Aloe secundiflora Crude Extracts on Ascaridia galli in Vitro

2012 ◽  
Vol 2 (2) ◽  
pp. 49 ◽  
Author(s):  
Fredrick Kaingu ◽  
Alfred Kibor ◽  
Rebecca Waihenya ◽  
Robert Shivairo ◽  
Lewis Mungai
Keyword(s):  
Phenolic Compounds ◽  
Crude Extract ◽  
Larval Stage ◽  
Chemical Compounds ◽  
Ascaridia Galli ◽  
Inhibitory Effects ◽  
The Family ◽  
Medicinal Properties ◽  
Main Components

<p><em>Aloe secundiflora </em>Synonym: <em>Aloe floramaculata, Aloe marsabitensis, Aloe engleri</em> belongs to the family <em>Asphodelaceae.</em> <em>Aloe</em> leaf gel and <em>Aloe</em> exudates are the main components. The gel is derived from parenchytomous cells while exudates are derived from the inner epidermal layers. The gel consists of mainly polysaccharides while the exudates consists of a mixture of phenolic compounds mainly athrones, chromones and phenyl pyrones. Leaf components of <em>Aloe</em> have been credited for antibacterial, antifungal and antiviral and anthelmintic medicinal properties. The effectiveness of <em>Aloe secundiflora </em>extracts on the most prevalent nematode of chicken <em>Ascaridia galli</em> was conducted <em>in vitro</em>. The results of this study indicate that Hexane, Ethylacetate, Acetone, Methanol and chloroform extracts were found active in hindering the development of <em>Ascaridia galli</em> eggs to larval stage three (L<sub>3</sub>), and this was dependent on the concentration of the crude extract. The lowest concentration of the various extracts (5 mg/ml) had an inhibition percent (IP), 75.52%, 79.60%, 87.21%, 86.13% and 43.6% respectively. The highest concentration of the extracts was (50 mg/ml), at this level the inhibition percent was found higher than in the lowest extracts concentrations i.e., 91.84%, 97.55%, 100%, 99.46% and 91.29% respectively. <em>Aloe secundiflora</em> extracts therefore have inhibitory effects on the <em>Ascaridia galli</em> larval development <em>in vitro</em>. Phytochemical tests on the extracts revealed the presence of various chemical compounds.</p>

scholarly journals Baijiu Vinasse Extract Scavenges Glyoxal and Inhibits the Formation of Nε-Carboxymethyllysine in Dairy Food

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1526 ◽  
Author(s):  
Yuzhen Wang ◽  
Huilin Liu ◽  
Dianwei Zhang ◽  
Jingmin Liu ◽  
Jing Wang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acid ◽  
Inhibitory Effect ◽  
Acetone Extract ◽  
Inhibitory Mechanism ◽  
Inhibitory Effects ◽  
Dairy Food ◽  
Dairy Foods

The inhibitory effects of baijiu vinasse extract and its phenolic acid compounds on the Nε-carboxymethyllysine (CML) formation from dairy food were investigated. The inhibitory effect of the baijiu vinasse extract against CML formation was 43.2% in the casein and D-glucose model, which used 6 mL of the 70% acetone extract at 60 °C for 40 min. The HPLC-MS/MS profiles of the vinasse extract indicated that vanillic, chlorogenic, p-coumaric, sinapic, caffeic, ferulic, and syringic acids were seven major phenolic acid compounds. Furthermore, the inhibitory mechanism of the phenolic acid compounds in the model of dairy food was discussed by the trapping and scavenging of glyoxal. The results of this study exhibit that seven major antioxidant phenolic acid compounds may play important roles in the antioxidant activity and CML inhibition of the vinasse extract in a model of dairy foods.

In Vitro Effects of Ethanol on Rabbit Platelet Aggregation, Secretion of Granule Contents, and Cyclic AMP Levels in the Presence of Prostacyclin

1989 ◽  
Vol 61 (02) ◽  
pp. 254-258 ◽  
Author(s):  
Margaret L Rand ◽  
Peter L Gross ◽  
Donna M Jakowec ◽  
Marian A Packham ◽  
J Fraser Mustard
Keyword(s):  
Cyclic Amp ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Inhibitory Effects ◽  
Low Concentrations ◽  
Rabbit Platelets ◽  
High Concentrations ◽  
In Vitro Effects ◽  
Aggregation Of Platelets

SummaryEthanol, at physiologically tolerable concentrations, inhibits platelet responses to low concentrations of collagen or thrombin, but does not inhibit responses of washed rabbit platelets stimulated with high concentrations of ADP, collagen, or thrombin. However, when platelet responses to high concentrations of collagen or thrombin had been partially inhibited by prostacyclin (PGI2), ethanol had additional inhibitory effects on aggregation and secretion. These effects were also observed with aspirin- treated platelets stimulated with thrombin. Ethanol had no further inhibitory effect on aggregation of platelets stimulated with ADP, or the combination of ADP and epinephrine. Thus, the inhibitory effects of ethanol on platelet responses in the presence of PGI2 were very similar to its inhibitory effects in the absence of PGI2, when platelets were stimulated with lower concentrations of collagen or thrombin. Ethanol did not appear to exert its inhibitory effects by increasing cyclic AMP above basal levels and the additional inhibitory effects of ethanol in the presence of PGI2 did not appear to be brought about by further increases in platelet cyclic AMP levels.

Inhibition of ADP-Induced Responses in Human Platelets by Agents Elevating the Cyclic AMP Level: Comparison of Aggregation and Shape Change

1984 ◽  
Vol 52 (03) ◽  
pp. 333-335 ◽  
Author(s):  
Vider M Steen ◽  
Holm Holmsen
Keyword(s):  
Inhibitory Action ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Shape Change ◽  
Inhibitory Effect ◽  
Human Platelets ◽  
Inhibitory Effects ◽  
Early Step ◽  
Stimulus Response ◽  
Sequential Relationship

SummaryThe inhibitory effect of cAMP-elevating agents on shape change and aggregation in human platelets was studied to improve the understanding of the sequential relationship between these two responses.Human platelet-rich plasma was preincubated for 2 min at 37° C with prostaglandin E1 or adenosine, agents known to elevate the intracellular level of cAMP. Their inhibitory effects on ADP-induced shape change and aggregation were determined both separately and simultaneously. The dose-inhibition patterns for shape change and aggregation were similar for both PGE1 and adenosine. There was no distinct difference between the inhibitory action of these two inhibitors.These observations suggest that elevation of the intracellular concentration of cAMP interferes with an early step in the stimulus-response coupling that is common for aggregation and shape change.

The Inhibitory Effect of Heparin and Related Glycosaminoglycans on Neutrophil Chemotaxis

1984 ◽  
Vol 52 (02) ◽  
pp. 134-137 ◽  
Author(s):  
Yaacov Matzner ◽  
Gerard Marx ◽  
Ruth Drexler ◽  
Amiram Eldor
Keyword(s):  
Specific Binding ◽  
Anticoagulant Activity ◽  
Neutrophil Migration ◽  
Inhibitory Effect ◽  
Chemotactic Factor ◽  
Human Neutrophils ◽  
Boyden Chamber ◽  
Neutrophil Chemotaxis ◽  
Inhibitory Effects ◽  
Chemotactic Factors

SummaryClinical observations have shown that heparin has antiinflammatory activities. The effect of heparin on neutrophil chemotaxis was evaluated in vitro in the Boyden Chamber. This method enabled differentiation between the direct effects of heparin on neutrophil migration and locomotion, and its effects on chemotactic factors. Heparin inhibited both the random migration and directed locomotion of human neutrophils toward zymosan-activated serum (ZAS) and F-met-leu-phe (FMLP). Inhibition was found to be dependent on the concentrations of the heparin and of the chemotactic factors. No specific binding of heparin to the neutrophils could be demonstrated, and heparin’s inhibitory effects were eliminated by simple washing of the cells. When added directly to the chamber containing chemotactic factor, heparin inhibited the chemotactic activity of ZAS but not that of FMLP, suggesting a direct inhibitory effect against C5a, the principal chemotactic factor in ZAS.Experiments performed with low-molecular-weight heparin, N-desulfated heparin, dextran sulfate, chondroitin sulfate and dextran indicated that the inhibitory effects of heparin on neutrophil chemotaxis are not related to its anticoagulant activity, but probably depend on the degree of sulfation of the heparin molecule.

A comparative study of the antibacterial activity of Quercus robur (Ethanolic extract) and Zinc oxide nanoparticles on Salmonella (In vitro)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Hams H. H. Alfattli ◽  
Ghufran Zuhair Jiber ◽  
Ghaidaa Gatea Abbass
Keyword(s):  
Zinc Oxide ◽  
Antibacterial Activity ◽  
Zinc Oxide Nanoparticles ◽  
Quercus Robur ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Oxide Nanoparticles ◽  
Inhibition Zones

This study which designed to evaluate the inhibitory effect of Ethanolic extract of (Quercusrobur) and Zinc oxide nanoparticles on the growth of one genus of enterobacteriacae (Salmonella). In vitro. For this purpose graduate concentrates for plant extract (50, 100, 200, 400 )mg/ml which prepared and compared with Zinc oxide nanoparticles of different concentration (2, 1, 0.5, 0.25) μg/ml,and examined. The result showed that the studied medicinal plant has antibacterial activity against this bacteria which used. The result showed that the plant has good activity in decrease the growth of this bacteria. The results of the study also showed that the nano-ZnO has very effective antibacterial action against the studied bacteria which was Salmonella,nanoparticles concentrations lead to increasing in the inhibition zones of tested bacterial growth. We also study the effect of three antibiotics Lomefloxacin (LOM), Ciprofloxacin (SIP) and Rifampin (RA) and the result showed,in a comparison within the tested bacteria,Salmonella had a significant inhibition increase in Lomefloxacin ; the ciprofloxacin showed effect on tested bacteria. However,Rifampin does not show any effect on tested bacteria.

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