scholarly journals D-Glucosamine Conjugation Accelerates the Labeling Efficiency of Quantum Dots in Osteoblastic Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Kazunari Igawa ◽  
Ming-Fang Xie ◽  
Hideki Ohba ◽  
Shizuka Yamada ◽  
Yoshihiko Hayashi
Keyword(s):  
Quantum Dots ◽  
Emission Spectra ◽  
Osteoblastic Cells ◽  
Core Size ◽  
Fluorescent Staining ◽  
Broad Absorption ◽  
Dramatic Decrease ◽  
High Fluorescence Intensity ◽  
High Fluorescence ◽  
Narrow Emission

Quantum dots (QDs) are useful imaging tools in the medical and biological fields due to their optical properties, such as a high fluorescence intensity, remarkable resistance to photobleaching, broad absorption spectra, and narrow emission spectra. This is the first study to investigate the uptake of carboxylated QDs conjugated with D-glucosamine (core size: approximately 3 nm, final modified size: 20–30 nm) into cultured osteoblastic cells. The QDs attached to the cell surface and were transported into the cytoplasm within approximately three hours of culture, whose process was clearly demonstrated using specific fluorescent staining of the cell membrane. Although the intranuclear distribution was not observed, a dramatic decrease in the transfer of quantum dots into the cytoplasm was recognized after approximately seven days of culture. Other interesting phenomena include the escape of the quantum dots from lysosomes in the cytoplasm, as confirmed by the merging of both QD fluorescence and specific fluorescent staining of lysosomes in the cytoplasm. These findings suggest that D-glucosamine conjugation enhances proton absorption in acid organelles and promotes the lysosomal escape of QDs.

scholarly journals Quantum Dots—Assisted 2D Fluorescence for Pattern Based Sensing of Amino Acids, Oligopeptides and Neurotransmitters

Sensors ◽  
2019 ◽  
Vol 19 (17) ◽  
pp. 3655 ◽  
Author(s):  
Marcin Zabadaj ◽  
Patrycja Ciosek-Skibińska
Keyword(s):  
Amino Acids ◽  
Quantum Dots ◽  
Emission Spectra ◽  
Specific Interactions ◽  
High Quantum Yield ◽  
Huge Amount ◽  
Broad Absorption ◽  
Amount Of Information ◽  
Decay Times ◽  
Proof Of Principle

Quantum dots (QDs) are very attractive nanomaterials for analytical chemistry, due to high photostability, large surface area featuring numerous ways of bioconjugation with biomolecules, usually high quantum yield and long decay times. Their broad absorption spectra and narrow, sharp emission spectra of size-tunable fluorescence make them ideal tools for pattern-based sensing. However, almost always they are applied for specific sensing with zero-dimensional (0D) signal reporting (only peak heights or peak shifts are considered), without taking advantage of greater amount of information hidden in 1D signal (emission spectra), or huge amount of information hidden in 2D fluorescence maps (Excitation-Emission Matrixes, EEMs). Therefore, in this work we propose opposite strategy—non-specific interactions of QDs, which are usually avoided and regarded as their disadvantage, were exploited here for 2D fluorescence fingerprinting. Analyte-specific multivariate fluorescence response of QDs is decoded with the use of Partial Least Squares—Discriminant Analysis. Even though only one type of QDs is studied, the proposed pattern-based method enables to obtain satisfactory accuracy for all studied compounds—various neurotransmitters, amino-acids and oligopeptides. This is a proof of principle of the possibility of the identification of various bioanalytes by such fluorescence fingerprinting with the use of QDs.

scholarly journals CdSe Quantum Dots in Human Models Derived from ALS Patients: Characterization, Nuclear Penetration Studies and Multiplexing

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 671
Author(s):  
Carlota Tosat-Bitrián ◽  
Alicia Avis-Bodas ◽  
Gracia Porras ◽  
Daniel Borrego-Hernández ◽  
Alberto García-Redondo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Molecular Mechanisms ◽  
Emission Spectra ◽  
Molecular Profiling ◽  
Proper Function ◽  
Cdse Quantum Dots ◽  
Cdse Qds ◽  
Als Patients ◽  
Lateral Sclerosis ◽  
Narrow Emission

CdSe quantum dots (QDs) are valuable tools for deciphering molecular mechanisms in cells. Their conjugation with antibodies offers a unique staining source with optimal characteristics, including increased photostability and narrow emission spectra, allowing for improved multiplexing capabilities using a single excitation source. In combination with pathology models derived from patients, they have great potential to contribute to quantitative molecular profiling and promote personalized medicine. However, the commercial availability of diverse CdSe QDs is still limited and characterization techniques must be performed to these materials or the conjugates developed in the lab to assure a proper function and reproducibility. Furthermore, while there is significant data of QDs experiments in cell lines, the literature with primary human cells is scarce, and QD behavior in these systems may be different. Rigorous characterization data of commercially available QDs and their conjugates with biomolecules of interest is needed in order to establish their potential for target labelling and expand their use among research labs. Here we compare the characterization and labelling performance of different QD conjugates in SH-SY5Y cell line, fibroblasts and immortalized lymphocytes derived from amyotrophic lateral sclerosis patients.

Imaging Takes a Quantum Leap

Physiology ◽  
2004 ◽  
Vol 19 (6) ◽  
pp. 322-325 ◽  
Author(s):  
Diane S. Lidke ◽  
Donna J. Arndt-Jovin
Keyword(s):  
Quantum Dots ◽  
Surface Chemistry ◽  
Fluorescence Imaging ◽  
Emission Spectra ◽  
Quantum Leap ◽  
In Vivo Fluorescence Imaging ◽  
Biomolecular Conjugation ◽  
Narrow Emission

Semiconducting nanocrystals, or quantum dots (QDs), have emerged as a new tool in physiological imaging, combining high brilliance, photostability, broad excitation but very narrow emission spectra, and surface chemistry compatible with biomolecular conjugation. In this review, we demonstrate the power of QDs in diverse applications, including long-term in vivo fluorescence imaging.

Rational design of fluorescent barcodes for suspension array through a simple simulation strategy

The Analyst ◽  
2021 ◽  
Author(s):  
Bo Zhang ◽  
Wan-Sheng Tang ◽  
Shou-Nian Ding
Keyword(s):  
Quantum Dots ◽  
Size Distribution ◽  
Fluorescence Intensity ◽  
Rational Design ◽  
Suspension Array ◽  
Simple Simulation ◽  
Simulation Strategy ◽  
High Fluorescence Intensity ◽  
High Fluorescence ◽  
Excellent Stability

The quantum dots (QDs)-encoded microbeads as optical barcode with high fluorescence intensity and fluorescence uniformity, excellent stability and dispersity are greatly important for suspension array (SA). However, the size distribution...

scholarly journals The bright future: Imaging dynamic cellular events with quantum dots

2010 ◽  
Vol 32 (3) ◽  
pp. 12-17 ◽  
Author(s):  
Andrew M. Smith ◽  
Mary M. Wen ◽  
Shuming Nie
Keyword(s):  
Quantum Dots ◽  
Light Emission ◽  
Emission Spectra ◽  
Semiconductor Quantum Dots ◽  
Broad Absorption ◽  
Light Emitting ◽  
Simultaneous Excitation ◽  
New Class ◽  
Cellular Events ◽  
Optical And Electronic Properties

Semiconductor quantum dots (QDs) are tiny light-emitting particles that have emerged as a new class of fluorescent labels for biology and medicine. Compared with traditional fluorescent probes, QDs have unique optical and electronic properties such as size-tuneable light emission, narrow and symmetric emission spectra, and broad absorption spectra that enable the simultaneous excitation of multiple fluorescence colours.

scholarly journals АССМОТРЕНИЕ ВЛИЯНИЯ САМОАГРЕГИРОВАНИЯ В НАНОРАЗМЕРНЫХ КЛАСТЕРАХ CDS

2021 ◽  
Vol 78 (3) ◽  
pp. 51-60
Author(s):  
A.M. Assilbekova ◽  
◽  
A.A. Aldongarov ◽  
I.S. Irgibaeva ◽  
◽  
...  
Keyword(s):  
Quantum Dots ◽  
Dipole Moment ◽  
Cadmium Sulfide ◽  
Density Functional ◽  
Electronic Absorption Spectra ◽  
Semiconductor Nanocrystals ◽  
Emission Spectra ◽  
Cds Nanoparticles ◽  
Trap States ◽  
Narrow Emission

Quantum dots, such as cadmium sulfide (CdS), are semiconductor nanocrystals that possess unique optical properties, including wide­range excitation, size­tunable narrow emission spectra and high pho­tostability. The size and composition of quantum dots can be varied to obtain the desired emission prop­erties and make them suitable for various optical and biomedical applications. In this article, the effect of self­aggregation on the electronic absorption spectra and on the dipole moment in CdS nanoparticles is considered using computer modeling methods based on the density functional tight­binding (DFTB). The object of the study is four CdS structures and two models of an aggregated dimer for each cluster. The construction of dimers of cadmium sulfide clusters showed that a higher level of passivation can be achieved in comparison with the initial monomers. In this case, the construction of dimers should occur along the direction of the dipole moment of the monomer in order to minimize it. Therefore, it can be assumed that the dipole moment plays a key role in the formation of trap states in nanosized clusters of cadmium sulfide, and the problem of passivation is reduced to minimizing the dipole moment.

scholarly journals Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications

2019 ◽  
Vol 12 (05) ◽  
pp. 1940003 ◽  
Author(s):  
Mengjie Zhao ◽  
Shuai Ye ◽  
Xiao Peng ◽  
Jun Song ◽  
Junle Qu
Keyword(s):  
Quantum Dots ◽  
Fluorescence Lifetime ◽  
Super Resolution ◽  
Stimulated Emission ◽  
Lateral Resolution ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
High Fluorescence Intensity ◽  
High Fluorescence

Inorganic quantum dots (QDs) have excellent optical properties, such as high fluorescence intensity, excellent photostability and tunable emission wavelength, etc., facilitating them to be used as labels and probes for bioimaging. In this study, CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope (FLIM) and stimulated emission depletion (STED) nanoscopy imaging. The emission peak of CdSe@ZnS QDs centered at 526[Formula: see text]nm with a narrow width of 19[Formula: see text]nm and the photoluminescence quantum yield (PLQY) was 64%. The QDs presented excellent anti-photobleaching property which can be irradiated for 400[Formula: see text]min by STED laser with 39.8[Formula: see text]mW. The lateral resolution of 42.0[Formula: see text]nm is demonstrated for single QDs under STED laser (27.5[Formula: see text]mW) irradiation. Furthermore, the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5[Formula: see text]nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD probes. Meanwhile, Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM, and their fluorescence lifetime was around 3.1[Formula: see text]ns, consistent with the in vitro value, suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.

scholarly journals Quick extracellular biosynthesis of low-cadmium ZnxCd1−xS quantum dots with full-visible-region tuneable high fluorescence and its application potential assessment in cell imaging

RSC Advances ◽  
2021 ◽  
Vol 11 (35) ◽  
pp. 21813-21823
Author(s):  
Shiyue Qi ◽  
Ji Chen ◽  
Xianwei Bai ◽  
Yahui Miao ◽  
Shuhui Yang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Saving ◽  
Metal Nanoparticles ◽  
Cell Imaging ◽  
Visible Region ◽  
Extracellular Biosynthesis ◽  
Application Potential ◽  
Good Biocompatibility ◽  
High Fluorescence ◽  
Environmentally Sound

The biosynthesis of metal nanoparticles/QDs has been universally recognized as environmentally sound and energy-saving, generating less pollution and having good biocompatibility, which is most needed in biological and medical fields.

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