scholarly journals 3D Culture of MIN-6 Cells on Decellularized Pancreatic Scaffold: In Vitro and In Vivo Study

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Di Wu ◽  
Jian Wan ◽  
Yan Huang ◽  
Yibing Guo ◽  
Tianxin Xu ◽  
...  
Keyword(s):  
Cultured Cells ◽  
3D Culture ◽  
Normal Function ◽  
Diabetic Patients ◽  
3D Scaffold ◽  
Cell Engraftment ◽  
Type 1 Diabetic Patients

Type 1 diabetes is an autoimmune disease which is due to the lack ofβcells. The ideal therapy to cure the disease is pancreas transplantation, but its application is confined to a limited number of people due to the shortage of organ and the need for life-long immunosuppression. Regenerative medicine methods such as a tissue engineered pancreas seem to provide a useful method. In order to construct a microenvironment similar to the native pancreas that is suitable for not only cell growth but also cellular function exertion, a decellularized mouse pancreas was used as a natural 3D scaffold in this experiment. MIN-6βcells were planted in the bioscaffold. The cell engraftment was verified by HE staining and SEM. Immunostaining procedures were performed to confirm the normal function of the engrafted cells. qRT-PCR demonstrated that insulin gene expression of the recellularized pancreas was upregulated compared with conventional plate-cultured cells. In vivo experiment was also accomplished to further evaluate the function of the recellularized bioscaffold and the result was inspiring. And beyond doubt this will bring new hope for type 1 diabetic patients.

Activation of human aortic endothelial cells by LDL from Type 1 diabetic patients: an in vitro study

2002 ◽  
Vol 165 (1) ◽  
pp. 69-77 ◽  
Author(s):  
Rosa A Rabini ◽  
Arianna Vignini ◽  
Eleonora Salvolini ◽  
Roberto Staffolani ◽  
Daniela Martarelli ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Diabetic Patients ◽  
Aortic Endothelial Cells ◽  
Type 1 Diabetic Patients ◽  
Human Aortic Endothelial Cells ◽  
Aortic Endothelial

Low-density lipoproteins from type 1 diabetic patients induce in vitro endothelial dysfunction

1999 ◽  
Vol 144 ◽  
pp. 57
Author(s):  
R.A. Rabini ◽  
R. Staffolani ◽  
E. Salvolini ◽  
D. Martarelli ◽  
A. Vignini
Keyword(s):  
Endothelial Dysfunction ◽  
Low Density Lipoproteins ◽  
Diabetic Patients ◽  
Low Density ◽  
Type 1 Diabetic Patients

scholarly journals Low frequency of GITR+ T cells in ex vivo and in vitro expanded Treg cells from type 1 diabetic patients

2013 ◽  
Vol 25 (10) ◽  
pp. 563-574 ◽  
Author(s):  
Cristina Xufré ◽  
Manuela Costa ◽  
Carme Roura-Mir ◽  
Eva Codina-Busqueta ◽  
Lorena Usero ◽  
...  
Keyword(s):  
T Cells ◽  
Ex Vivo ◽  
Low Frequency ◽  
Treg Cells ◽  
Diabetic Patients ◽  
Type 1 Diabetic Patients

The regenerative capacity of the notochordal cell: tissue constructs generated in vitro under hypoxic conditions

2009 ◽  
Vol 10 (6) ◽  
pp. 513-521 ◽  
Author(s):  
W. Mark Erwin ◽  
Facundo Las Heras ◽  
Diana Islam ◽  
Michael G. Fehlings ◽  
Robert D. Inman
Keyword(s):  
Extracellular Matrix ◽  
Tissue Culture ◽  
Sodium Alginate ◽  
Cultured Cells ◽  
3D Culture ◽  
Culture Conditions ◽  
Notochordal Cells ◽  
Collagen Ii

Object The intervertebral disc (IVD) is a highly avascular structure that is occupied by highly specialized cells (nucleus pulposus [NP] cells) that have adapted to survive within an O2 concentration of 2–5%. The object of this study was to investigate the effects of long-term hypoxic and normoxic tissue cultures of nonchondrodystrophic canine notochordal cells—cells that appear to protect the disc NP from degenerative change. Methods The authors obtained notochordal cells from nonchondrodystrophic canines according to their established methods and placed them into monolayer and 3D culture using sodium alginate globules under either hypoxic (3.5% O2) or normoxic (21% O2) conditions. Histological, immunohistochemical, scanning electron microscopy, and histomorphometric methods were used to evaluate the cells within the globules after 5 months in culture. Results Notochordal cells under in vitro hypoxic tissue culture conditions produced a highly complex, organized, 3D cellular construct that was strikingly similar to that observed in vivo. In contrast, traditional normoxic tissue culture conditions resulted in notochordal cells that failed to produce an organized matrix. Hypoxia resulted in a matrix rich in aggrecan and collagen II, whereas normoxic cultured cells did not produce any observable aggrecan or collagen II after 5 months of culture. Conclusions Hypoxia induces notochordal cells to organize a complex 3D cellular/extracellular matrix without an external scaffold other than suspension within sodium alginate. These cells produce an extracellular matrix and large construct that shares exactly the same characteristics as the in vivo condition—robust aggrecan, and type II collagen production. Normoxic tissue culture conditions, however, lead to a failure of these cells to thrive and a lack of extracellular matrix production and significantly smaller cells. The authors suggest that future studies of NP cells and, in particular, notochordal cells should utilize hypoxic tissue culture conditions to derive meaningful, biologically relevant conclusions concerning possible biological/molecular interventions.

scholarly journals Generation of stem cell-derived β-cells from patients with type 1 diabetes

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Jeffrey R. Millman ◽  
Chunhui Xie ◽  
Alana Van Dervort ◽  
Mads Gürtler ◽  
Felicia W. Pagliuca ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Biology ◽  
Disease Model ◽  
Diabetic Patients ◽  
Β Cells ◽  
Β Cell ◽  
Diabetes Drug

Abstract We recently reported the scalable in vitro production of functional stem cell-derived β-cells (SC-β cells). Here we extend this approach to generate the first SC-β cells from type 1 diabetic patients (T1D). β-cells are destroyed during T1D disease progression, making it difficult to extensively study them in the past. These T1D SC-β cells express β-cell markers, respond to glucose both in vitro and in vivo, prevent alloxan-induced diabetes in mice and respond to anti-diabetic drugs. Furthermore, we use an in vitro disease model to demonstrate the cells respond to different forms of β-cell stress. Using these assays, we find no major differences in T1D SC-β cells compared with SC-β cells derived from non-diabetic patients. These results show that T1D SC-β cells could potentially be used for the treatment of diabetes, drug screening and the study of β-cell biology.

scholarly journals Neither Excessive Nitric Oxide Accumulation nor Acute Hyperglycemia Affects the N-Acetylaspartate Network in Wistar Rat Brain Cells

2020 ◽  
Vol 21 (22) ◽  
pp. 8541
Author(s):  
Marlena Zyśk ◽  
Piotr Pikul ◽  
Robert Kowalski ◽  
Krzysztof Lewandowski ◽  
Monika Sakowicz-Burkiewicz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Wistar Rat ◽  
Diabetic Patients ◽  
Mrna Levels ◽  
Male Adult ◽  
The Impact

The N-acetylaspartate network begins in neurons with N-acetylaspartate production catalyzed by aspartate N-acetyltransferase from acetyl-CoA and aspartate. Clinical studies reported a significant depletion in N-acetylaspartate brain level in type 1 diabetic patients. The main goal of this study was to establish the impact of either hyperglycemia or oxidative stress on the N-acetylaspartate network. For the in vitro part of the study, embryonic rat primary neurons were treated by using a nitric oxide generator for 24 h followed by 6 days of post-treatment culture, while the neural stem cells were cultured in media with 25–75 mM glucose. For the in vivo part, male adult Wistar rats were injected with streptozotocin (65 mg/kg body weight, ip) to induce hyperglycemia (diabetes model) and euthanized 2 or 8 weeks later. Finally, the biochemical profile, NAT8L protein/Nat8l mRNA levels and enzymatic activity were analyzed. Ongoing oxidative stress processes significantly affected energy metabolism and cholinergic neurotransmission. However, the applied factors did not affect the N-acetylaspartate network. This study shows that reduced N-acetylaspartate level in type 1 diabetes is not related to oxidative stress and that does not trigger N-acetylaspartate network fragility. To reveal why N-acetylaspartate is reduced in this pathology, other processes should be considered.

scholarly journals Autologous culture method improves retention of tumors’ native properties

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yao Tang ◽  
Qian Xu ◽  
Meiling Yan ◽  
Yimin Zhang ◽  
Ping Zhu ◽  
...  
Keyword(s):  
Drug Testing ◽  
Drug Sensitivity ◽  
Cultured Cells ◽  
Mesenchymal Cell ◽  
Culture Method ◽  
Autologous Serum ◽  
Clinical Samples ◽  
3D Scaffold

AbstractNo current in vitro tumor model replicates a tumor’s in vivo microenvironment. A culturing technique that better preserves a tumor’s pathophysiological conditions is needed for some important clinical applications, including personalized drug-sensitivity/resistance assays. In this study, we utilized autologous serum or body fluid to build a 3D scaffold and grow a patient’s tumor. We named this technique “3D-ACM” (autologous culture method). Forty-five clinical samples from biopsies, surgically removed tumor tissues and malignant body fluids were cultured with 3D-ACM. Traditional 3D-FBS (fetal bovine serum) cultures were performed side-by-side for comparison. The results were that cells cultured in 3D-ACM rebuilt tissue-like structures, and retained their immuno-phenotypes and cytokine productions. In contrast, the 3D-FBS method promoted mesenchymal cell proliferation. In preliminary chemo drug-sensitivity assays, significantly higher mortality was always associated with FBS-cultured cells. Accordingly, 3D-ACM appears to more reliably preserve a tumor’s biological characteristics, which might improve the accuracy of drug-testing for personalized cancer treatment.

scholarly journals Cellular basis of diabetic nephropathy: III. In vitro GLUT1 mRNA expression and risk of diabetic nephropathy in Type 1 diabetic patients

Diabetologia ◽  
2004 ◽  
Vol 47 (10) ◽  
pp. 1789-1794 ◽  
Author(s):  
C. Huang ◽  
Y. Kim ◽  
M. L. Caramori ◽  
A. J. Fish ◽  
S. S. Rich ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Mrna Expression ◽  
Diabetic Patients ◽  
Cellular Basis ◽  
Type 1 Diabetic Patients ◽  
Glut1 Mrna
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