scholarly journals Effect of Low and High Dose Sugammadex on the Coagulation and Fibrinolytic System in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Nilay Taş ◽  
Özgür Yağan ◽  
Tevfik Noyan ◽  
Sema Nur Ayyıldız ◽  
Y. Burcu Üstün ◽  
...  
Keyword(s):  
Dose Group ◽  
Clinical Results ◽  
Fibrinolytic System ◽  
Control Group ◽  
High Dose ◽  
Reversal Agent ◽  
Group Vi ◽  
Group V ◽  
Coagulation Parameters ◽  
Group I

Background. Sugammadex is a new reversal agent that has entered use recently. It is known that with sugammadex some changes in coagulation parameters occur without documented clinical results. Aim of the Work. The objective of this study was to identify effects of sugammadex on liver functions, coagulation, and fibrinolytic systems. Methods. Thirty-six rats were randomized into six groups: Group I, control; Group II, rocuronium group; Group III, sugammadex administered in 16 mg kg−1 dose; Group IV, sugammadex administered in 96 mg kg−1 dose; Group V, rocuronium and sugammadex administered in 16 mg kg−1 dose; and Group VI, rocuronium and sugammadex administered in 96 mg kg−1 dose. After 120 minutes, blood samples were obtained for prothrombin time, activated partial thromboplastin time, D-dimer, fibrinogen, aspartate aminotransferase, alanine aminotransferase, albumin, platelet, and mean platelet volume analyses. Results. Compared to the control group, in all groups measured parameters did not show any effect from a statistical viewpoint either due to the administered drugs alone or due to interaction effects. Conclusion. The conclusion was reached that administration of sugammadex in rats did not have any significant effect on the fibrinolytic system, coagulation parameters, and liver function.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

scholarly journals Chemoprevention with green propolis green propolis extracted in L-lysine versus carcinogenesis promotion with L-lysine in N-Butyl-N-[4-hydroxybutyl] nitrosamine (BBN) induced rat bladder cancer

2012 ◽  
Vol 27 (2) ◽  
pp. 185-192 ◽  
Author(s):  
Conceição Aparecida Dornelas ◽  
Francisco Vagnaldo Fechine-Jamacaru ◽  
Irineu Lima Albuquerque ◽  
Hemerson Iury Ferreira Magalhães ◽  
Adjair Jairo Silva de Souza ◽  
...  
Keyword(s):  
Group Iv ◽  
The Other ◽  
Control Group ◽  
Group Viii ◽  
Group Vi ◽  
Group V ◽  
Induction Group ◽  
Rat Bladder ◽  
Bladder Carcinogenesis ◽  
Group I

PURPOSE: To determine the effects of green propolis extracted in L-lysine (WSDP) and of L- lysine for 40 weeks on induced rat bladder carcinogenesis. METHODS: The animals (groups I, II, III, IV, V and VI) received BBN during 14 weeks. Group I was treated with propolis 30 days prior received BBN, and then these animals were treated daily with propolis; Groups II and III was treated with subcutaneous and oral propolis (respectively) concurrently with BBN. The animals of Group IV were treated L-lysine; Group V received water subcutaneous; and Group VI received only to BBN. Among the animals not submitted to carcinogenesis induction, Group VII received propolis, Group VIII received L-lysine and Group IX received water. RESULTS: The carcinoma incidence in Group I was lower than that of control (Group VI). The carcinoma multiplicity in Group IV was greater than in Group VI. All animals treated with L-lysine developed carcinomas, and they were also more invasive in Group IV than in controls. On the other hand, Group VIII showed no bladder lesions. CONCLUSION: The WSDP is chemopreventive against rat bladder carcinogenesis, if administered 30 days prior to BBN , and that L-lysine causes promotion of bladder carcinogenesis.

Role Of The Fibrinolytic System In Renal Transplantation

1981 ◽  
Author(s):  
P Glas-Greenwalt ◽  
M H Goldman
Keyword(s):  
Renal Transplantation ◽  
Endothelial Damage ◽  
Fibrinolytic System ◽  
The Other ◽  
Group Vi ◽  
Significant Drop ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Before And After

To determine the importance of the fibrinolytic system in renal transplantation on the one hand, and to establish a correlation between possible endothelial damage due to treatment of the renal graft and fibrinolytic parameters on the other, dogs were divided in six groups. Group I dogs were subjected to anesthesia only. Group II was sham operated. In group III, kidneys were perfused with the supernatant of either autologous or homologous cryo-precipitated plasma, and in group IV with albumin. In group V kidneys were cold stored. This was followed by autotransplantation. In group VI kidneys were perfused with albumin and allografted. Before and after transplantation, total plasma plasminogen (pro) activator activities in systemic and renal circulations were measured on fibrin plates after the addition of dextran sulfate and flufenamate to euglobulin fractions. Vascular activator (VA) was measured by adding Cl-inactivator. There was no marked difference between groups III, IV and VI. In comparing, however, group V with any of the perfused groups, an overall higher fibrinolytic activity was recorded both for intrinsic activators (p<.001) and VA (p<.001). in group I a significant drop in both activities (p<.01 -<.02) could be directly related to the duration of anesthesia with recovery within 24 hours. This pattern, though highly accentuated, revealed itself in all the other groups studied, with VA temporarily reaching zero-levels in the renal circulation in group VI. This data indicates the participation of the fibrinolytic system, in particular of the VA, in determining the fate of renal grafts.

An analgetic activity of sedges (Cyperus rotundus L.) extract on white male mice (Mus musculus L.)

2003 ◽  
Vol 1 (2) ◽  
pp. 50-57 ◽  
Author(s):  
HESTI PUSPITASARI ◽  
SHANTI LISTYAWATI ◽  
TETRI WIDIYANI
Keyword(s):  
White Male ◽  
Cyperus Rotundus ◽  
Control Group ◽  
Root Extract ◽  
Group Vi ◽  
Group V ◽  
Thermal Pain ◽  
Group I ◽  
The Difference ◽  
The Way

The objectives of the research were to find out the effect of giving sedges root extract orally on the number of writhing after chemical pain induction and time reaction after thermal pain induction of mice and also to find out the extract dosage which had an influence on decreasing number of writhing after chemical pain induction and length of reaction time after thermal pain induction of mice. The Complete Random Design (CRD) with 6 treatment groups and each treatment used 5 repetitions were used in this study. The groups were: Group I , control group, treated with sedges root extract of 0 mg/ 20 g BW , 0,5 ml; Group II treated with sedges root extract of 1 mg/ 20 g BW, 0,5 ml; Group III treated with sedges root extract of 3 mg/ 20 g BW, 0,5 ml; Group IV treated with sedges root extract of 5 mg/ 20 g BW, 0,5 ml; Group V treated with sedges root extract of 7 mg/ 20 g BW, 0,5 ml; Group VI treated with asetosal 200 mg/ kg BW , 0,5 ml/ 20 g BW and for the activity test, the sedges root extract was suspended in CMC 1%. Induction of chemical pain was done according to Witkin et al. (1965) in Pudjiastuti et al. (2000), in which 0,1 ml 3% of Acetic Acid/ 20 g BB was injected intraperitoneally 30 minutes after giving oral-material test. The mouse gave a respond in the way of writhing. Thermal pain induction was done by placing the mouse on hot plate with constant temperature of 55oC. The mouse gave a respond in the way of lick its back foot or even jumping. The data collected was analyzed using one direction ANOVA model and it was continued with LSD test in order to find out the difference every treatment group. The result of the analysis showed that the sedges root extract dosage of 7 mg/ 20 g BB decreased the number of writhing after chemical pain induction and length of mouse time reaction after thermal pain induction, so that sedges root extract dosage 7 mg/ 20 g BB had an analgetic function.

scholarly journals Liposome Encapsulated Astaxanthin Altered Biochemical Profile In Diethylnitrosamine (DEN) Induced Hepato Carcinoma On Swiss Albino Mice

2020 ◽  
pp. 344-352
Author(s):  
Suganya Vasudevan ◽  
Anuradha Venkataraman
Keyword(s):  
Hepatocellular Carcinoma ◽  
Control Group ◽  
Group Vi ◽  
In Vivo Evaluation ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Inflammatory State ◽  
Abnormal Cells

Cancer is a disease in which a group of abnormal cells grow uncontrollably by disregarding the normal rules of cell division. Across several cancers, Hepatocellular carcinoma (HCC) is one of the most aggressive cancers in worldwide. It is held responsible for up to 1 million deaths globally per annum. HCC is an inflammation-related cancer, as a chronic inflammatory state is necessary for cancer appearance. In this study, the drug astaxanthin and encapsulated astaxanthin was tested against HCC. Mice were divided into 7 groups; Group I: control, Group II: DEN induced, Group III: DEN + 50 mg/kg astaxanthin, Group IV: DEN + 100 mg/kg astaxanthin, Group V: DEN + 50 mg/kg encapsulated astaxanthin, Group VI: DEN + 100 mg/kg encapsulated astaxanthin, Group VII: DEN + 10 mg/kg sorafenib. Regular diet was given. Body weight, Food intake, water intake was noted. Other biochemical parameters such as ALP, AST, Albumin, proteins and TNF-α was determined. Finally, the liver was removed from each mice of different group by sacrificing them and histopathology was done. In vivo evaluation in mice models showed significant antitumor activities by both encapsulated and non-encapsulated astaxanthin at 100 mg/kg as compared with the control, DEN induced group and positive drug sorafenib. This research suggested that encapsulated astaxanthin can also be used as chemotherapeutic agent for the treatment of Hepatocellular carcinoma (HCC).

scholarly journals Evaluating the Effects of Different Doses of Vitamin B2 and Single Dose of Vitamin B12 Against Myelosuppression Induced by Cyclophosphamide in Experimental Rats

2020 ◽  
Vol 29 (1) ◽  
pp. 134-142
Author(s):  
Waleed K. Ghanim ◽  
Nada N. Al-Shawi
Keyword(s):  
Vitamin B12 ◽  
Treated Group ◽  
Control Group ◽  
Vitamin B2 ◽  
Group Vi ◽  
Adult Rats ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Group Ii

Cyclophosphamide is chemotherapeutic agent that utilized for the treatment of different malignancies; however its’ used associated with numerous adverse effects. Vitamin B2 and vitamin B12 suggested having myeloprotective effect. This work is designed to investigate the myeloprotective effect of both vitamins against cyclophosphamide induced myelosuppression. One hundred adult rats of both sexes were used in this study. The animals were randomly enrolled into ten groups of 10 rats each. Group I: Control group. Group II: Cyclophosphamide-treated. Group III and Group IV Orally-administered vitamin B2 (10, and 40 mg/kg/day), respectively alone for 7 days. Group V: Orally-administered vitamin B12 (0.1 mg/kg/day) alone for 7 days. Group VI and Group VII: Orally-administered vitamin B2 (10, and 40 mg/kg/day), respectively for 7 days and a single IP injection of cyclophosphamide (150 mg/kg) at day 7.Group VIII: Orally-administered vitamin B12 (0.1 mg/kg/day) for 7 days and a single IP injection of cyclophosphamide (150 mg/kg) at day 7. Group IX: Orally-administered a combination of vitamin B2 (10 mg/kg/day) and vitamin B12 (0.1 mg/kg/day) for 7 days and a single IP injection of cyclophosphamide (150 mg/kg) at day 7. Group X: orally-administered a combination of vitamin B2 (40 mg/kg/day) and vitamin B12 (0.1 mg/kg/day) for 7 days and a single IP injection of cyclophosphamide (150 mg/kg) at day 7. On day eight, animals were sacrificed and blood collected for CBCs and femur bone were extracted for bone marrow histological examination. Vitamin B2 and vitamin B12 significantly (P<0.05) increase CBCs; and the combination of vitamins produce -a significant (P<0.05) increase in CBCs compared to corresponding counts in other Groups, and -improve histopathological changes compared to Group II rats. In conclusion both vitamins may have myeloprotective effects against cyclophosphamide-induced myelosuppression.

scholarly journals Preserved Ovarian Function Following Toxicity With Doxorubicin in Rats: Protective Effect of Nasturtium Officinale Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 57-64
Author(s):  
Parastou Rad ◽  
◽  
Fahimeh Safari ◽  
Jamshid Mohammadi ◽  
Hamdollah Delaviz ◽  
...  
Keyword(s):  
Ovarian Function ◽  
Serum Levels ◽  
Saline Group ◽  
Female Rats ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Nasturtium Officinale ◽  
Group I ◽  
The Mean

Background: Chemotherapy agents can cause ovarian dysfunction and eventually lead to infertility. This study investigated the effect of nasturtium officinale extract on the ovarian function following the toxicity induced by doxorubicin in female rats. Methods: Forty eight female Wistar rats (180-210g) were randomly divided in six groups as follows: Group I, normal rats receiving 1ml normal saline; Group II and III receiving 25 and 75 mg/kg of the extract daily by gavage for 21 days. Groups IV, V and VI receiving 10 mg/kg doxorubicin intraperitoneally on the first day. In addition, Group IV and V received 25 and 75 mg/kg of the extract, respectively. The serum levels of estrogen, progesterone, Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) and ovarian Malondialdehyde (MDA) were determined after 21 days of treatment. The mean numbers of various graafian follicles and corpus lutea were recorded after treatment. Results: The mean serum FSH level in Group VI (0.11±0.01) significantly reduced compared to those in Groups II (0.21±0.05) and III (0.23±0.01), (P<0.05). The mean serum LH and estrogen levels in Group VI (0.16±0.08) reduced insignificantly compared to those in the controls (0.21±0.02), and in Groups II (0.23±0.03) and III (0.22±0.09). A significant reduction in the number of primary, secondary and graafian follicles were observed in Group VI compared to the control group (P<0.05). The serum MDA level significantly declined in Group V compared to that in Group VI (P<0.05). Conclusion: The nasturtium officinale extract attenuated the toxic effect of doxorubicin on the rat ovaries and protected the cell division in the follicles and the oocytes maturation.

scholarly journals Comparative study of hypoglycemic activity of Morus alba with oral glibenclamide in streptozotocin induced diabetic rats

2019 ◽  
Vol 8 (3) ◽  
pp. 545
Author(s):  
Mamatha Reddy ◽  
Altaf Hussain Shaik
Keyword(s):  
Insulin Level ◽  
Stem Bark ◽  
Diabetic Rats ◽  
Morus Alba ◽  
Bark Extract ◽  
Control Group ◽  
Albino Rats ◽  
Group Vi ◽  
Group V ◽  
Group I

Background: Diabetes is a chronic metabolic disorder that continues to present a major worldwide health problem, characterized by absolute or relative deficiencies in insulin secretion and/or insulin action associated with chronic hyperglycaemia and disturbances of carbohydrate, lipid, and protein metabolism. It is fast growing disease, gains the status of a potential epidemic in India with prevalence of more than 62 million diabetic individuals currently diagnosed with the diabetes.Methods: The study was conducted at Department of Pharmacology, Kurnool Medical College, Kurnool for a period of 1 year from January 2017 to December 2018. Animals used were albino rats, of Wistar strain, weighing between 150-200gm of either sex. The animals were divided into six groups as: control group (I); pathogenic control group (II) injected intravenously (i.v.) with single dose of STZ (60mg/kg); Morus alba stem bark extract (group-III; 200mg/kg), and group-IV (400mg/kg); group-V animals treated with glibenclamide (5mg/kg, p.o.) following STZ treatment; group-VI, animals treated with bark extract per se (400 mg/kg).Results: The results of this study showed a significant decrease blood glucose level, glycosylated heamoglobin level, and reduction in glutathione and insulin level after STZ administration. These parameters were significantly (p<0.05) reversed by extracts dose dependently.Conclusions: Thus, authors conclude that M. alba stem bark extracts produced significant antidiabetic and antioxidant effect which might be due to the presence of bioactive components such as phenolic and flavonoid content in the extract. The study warrants the need for further evaluated in certain other models of diabetes.

scholarly journals INFLUENCE OF BONE MARROW MSCs ON THE DEVELOPMENT OF POSTTRANSPLANT CHANGES IN KIDNES

2016 ◽  
Vol 18 (1) ◽  
pp. 45-52 ◽  
Author(s):  
N. A. Onishchenko ◽  
S. S. Meshcherin ◽  
I. M. Ilyinsky ◽  
V. I. Sevastianov
Keyword(s):  
Nitrogen Excretion ◽  
Control Group ◽  
High Dose ◽  
Low Doses ◽  
Group V ◽  
Intact Control ◽  
Group I ◽  
Group Ii ◽  
High Doses ◽  
Kidney Autotransplantation

Aim:to study of infl uence of various doses of autologous BM MSCs on the development of chronic transplant nephropathy in a decentralized kidney using kidney autotransplantation model (KAT).Materials and methods.Five groups of experiments were performed on 105 Wistar rats. The model of kidney autotransplantation by means of surgical decentralization (denervation – delymphatization) and infl ammation induction with kidney antigen and Freund’s adjuvant was created in groups I, II and III. Group I served as a decentralization control (control 1). In groups II and III autologous BM MSCs were injected intravenously once 35–40 days after surgery – a high dose in group II: 3.0–5.0×106 cells; a low dose in group III: 0.3–0.5×106 cells; group IV served as intact control; group V served as intact control with the injection of the same dose of BM MSCs as in group II. Kidney excretory functions (diuresis, creatinine, urea, protein in blood and urine, sodium excretion) and morphology were examined during months 3, 5 and 7–10.Results.In all five groups over the study duration nitrogen excretion was not disrupted. High doses of BM MSCs after KAT modeling resulted after month 3 in pronounced proteinuria in all rats (3–3.5 times more than in group I) and gradually decreased diuresis; histologically severe focal cell infiltration and the accumulation of protein masses in lumina of glomeruli and tubules were observed. By month 10 glomerular and tubulointerstitial focal sclerosis was developed. Low doses of BM MSCs after KAT modeling led to gradual decrease of proteinuria after month 3 reaching the initial values by months 5 and 7 of observation; histologically rare foci of cellular infiltration around glomeruli were observed.Conclusion.A single application of low doses of BM MSCs is capable of protective desensitizing infl uence on the tissue of decentralized kidney and can prolong the duration of kidney function without signs of pronounced damage, while under the same conditions high doses of autologous BM MSCs lead to accelerated development of severe chronic transplant nephropathy.

scholarly journals Antioxidant and Hepatoprotective Effects of Irradiated Chicory Root on Liver Necrosis

2020 ◽  
Vol 11 (2) ◽  
Author(s):  
Fatemeh Salary ◽  
Abdollah Ramzani Ghara ◽  
Fereshteh Ezzati Ghadi ◽  
Kourosh Bamdad
Keyword(s):  
Gamma Ray ◽  
Control Group ◽  
Root Extract ◽  
Liver Necrosis ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Chicory Root ◽  
Group I ◽  
Male Wistar Rats

Background: Gamma irradiation has been recognized as a reliable and safe method for improving the bio-components of plants. Objectives: This study aimed to evaluate the effect of irradiated chicory root extract on liver necrosis. Methods: Twenty-four adult male Wistar rats were divided equally into six treatment groups: Group I) Control, Group II) CCl4, Group III) CCl4 + irradiated chicory root extract, Group IV) CCl4 + chicory root extract, Group V) irradiated chicory root extract, and Group VI) chicory root extract. Animals were treated for four weeks. At the end of the study, catalase, SOD, lipid peroxidation, ALT, AST, histopathology, and Fourier Transform Infrared Spectroscopy (FTIR) were evaluated. Results: The results showed that the elevated levels of LPO, ALT, and AST in the CCl4 group decreased due to irradiated chicory and chicory root extract. Also, molecular changes and abnormalities in liver tissue improved due to irradiated chicory and chicory root extract. Moreover, the results showed that irradiated chicory roots were more effective than non-irradiated chicory. Conclusions: In conclusion, gamma-ray can increase the bioactive components of chicory root and is more effective in the scavenging of free radicals that cause liver necrosis in CCl4-treated rats.

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