scholarly journals Particle Size Dependent Photodynamic Anticancer Activity of Hematoporphyrin-Conjugated Fe3O4Particles

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Ki Chang Nam ◽  
Kyong-Hoon Choi ◽  
Kyu-Dong Lee ◽  
Jung Hyun Kim ◽  
Jin-Seung Jung ◽  
...  
Keyword(s):  
Particle Size ◽  
Magnetic Nanoparticles ◽  
Anticancer Activity ◽  
Size Dependent ◽  
Anticancer Effects ◽  
Anticancer Treatment ◽  
Wet Chemical ◽  
Biological Entities ◽  
Technical Solutions ◽  
Better Than

Nanomedicine, which involves the use of magnetic nanoparticles such as Fe3O4, has provided novel technical solutions for cancer diagnosis and treatment. Most studies in nanomedicine have focused on the use of nanoparticles with magnetic resonance imaging and hyperthermia. However, to achieve optimum anticancer effects, it is important to understand the physicochemical properties of magnetic nanoparticles and their interactions with biological entities. In this study, we synthesized Fe3O4particles of various sizes and conjugated them with hematoporphyrin (HP) molecules by using a simple surface-modification method. HP molecules were covalently bound to the surface of Fe3O4particles by a wet chemical process, resulting in Fe3O4@HPs particles that were uniform in size, were nontoxic, and exhibited strong anticancer effects on human prostate cancer (PC-3) and breast cancer (MDA-MB-231) cell lines. The Fe3O4@HPs particles showed remarkable and efficient photodynamic anticancer activity, depending on their particle size. These results indicate that all size of Fe3O4@HPs particles can be useful for photodynamic anticancer therapy, although the smaller size is better than the larger size and further studies will be needed to confirm the potential for clinical anticancer treatment.

scholarly journals Continuous Size-Dependent Sorting of Ferromagnetic Nanoparticles in Laser-Ablated Microchannel

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yiqiang Fan ◽  
Feng Gao ◽  
Huawei Li ◽  
Gang Tang ◽  
Jian Zhuang ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Nanoparticles ◽  
Size Distribution ◽  
Rna Virus ◽  
Flow Direction ◽  
Low Cost ◽  
Size Dependent ◽  
Potential Applications ◽  
Size Sorting ◽  
Biological Entities

This paper reports a low-cost method of continuous size-dependent sorting of magnetic nanoparticles in polymer-based microfluidic devices by magnetic force. A neodymium permanent magnet was used to generate a magnetic field perpendicular to the fluid flow direction. Firstly, FeNi3 magnetic nanoparticles were chemically synthesized with diameter ranges from 80 nm to 200 nm; then, the solution of magnetic nanoparticles and a buffer were passed through the microchannel in laminar flow; the magnetic nanoparticles were deflected from the flow direction under the applied magnetic field. Nanoparticles in the microchannel will move towards the direction of high-gradient magnetic fields, and the degree of deflection depends on their sizes; therefore, magnetic nanoparticles of different sizes can be separated and finally collected from different output ports. The proposed method offers a rapid and continuous approach of preparing magnetic nanoparticles with a narrow size distribution from an arbitrary particle size distribution. The proposed new method has many potential applications in bioanalysis field since magnetic nanoparticles are commonly used as solid support for biological entities such as DNA, RNA, virus, and protein. Other than the size sorting application of magnetic nanoparticles, this approach could also be used for the size sorting and separation of naturally magnetic cells, including blood cells and magnetotactic bacteria.

scholarly journals Development of Coriandrum sativum Oil Nanoemulgel and Evaluation of Its Antimicrobial and Anticancer Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Ahmad M. Eid ◽  
Linda Issa ◽  
Omamah Al-kharouf ◽  
Raghad Jaber ◽  
Fatima Hreash
Keyword(s):  
Particle Size ◽  
Inhibitory Concentration ◽  
Antimicrobial Effect ◽  
Tween 80 ◽  
Carcinoma Cells ◽  
Coriandrum Sativum ◽  
Pharmaceutical Dosage Forms ◽  
Anticancer Effects ◽  
Carbopol 940 ◽  
Better Than

This study is aimed at developing coriander oil into a nanoemulgel and evaluating its antimicrobial and anticancer effects. Coriander (Coriandrum sativum) oil was developed into a nanoemulgel by using a self-nanoemulsifying technique with Tween 80 and Span 80. Hydrogel material (Carbopol 940) was then incorporated into the nanoemulsion and mixed well. After this, we evaluated the particle size, polydispersity index (PDI), rheology, antimicrobial effect, and cytotoxic activity. The nanoemulsion had a PDI of 0.188 and a particle size of 165.72 nm. Interesting results were obtained with the nanoemulgel against different types of bacteria, such as Pseudomonas aeruginosa, Klebsiella pneumoniae, and methicillin-resistant Staphylococcus aureus (MRSA), with a minimum inhibitory concentration (MIC) of 2.3 μg/ml, 3.75 μg/ml, and 6.5 μg/ml, respectively. In addition, the half-maximal inhibitory concentration (IC50) of the nanoemulgel when applying it to human breast cancer cells (MCF-7), hepatocellular carcinoma cells (Hep3B), and human cervical epithelioid carcinoma cells (HeLa) was 28.84 μg/ml, 28.18 μg/ml, and 24.54 μg/ml, respectively, which proves that the nanoemulgel has anticancer effects. The development of C. sativum oil into a nanoemulgel by using a self-nanoemulsifying technique showed a bioactive property better than that in crude oil. Therefore, simple nanotechnology techniques are a promising step in the preparation of pharmaceutical dosage forms.

scholarly journals Synthesis of 1-[(Aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic Acid Derivatives and Their Breast Anticancer Activity

Crystals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 571
Author(s):  
Ahmed Gaber ◽  
Walaa F. Alsanie ◽  
Majid Alhomrani ◽  
Abdulhakeem S. Alamri ◽  
Ibrahim M. El-Deen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Carboxylic Acid ◽  
Anticancer Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
Analytical Techniques ◽  
Anticancer Effect ◽  
Reference Compound ◽  
Anticancer Effects ◽  
Mcf 7

This research aimed to produce new 1-[(aryl)(3-amino-5-oxopyrazolidin-4-ylidene) methyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic acid derivatives and check their anticancer effect against the breast cancer MCF-7 cell line. The 2-oxo-1,2-dihydroquinoline-3-carboxylic acid (4) compound was obtained by hydrolyzing ethyl 2-oxo-1,2-dihydroquinoline-3-carboxylate (2) with thiourea and anhydrous potassium carbonate ethanol, which was then treated with ethyl 3-substituted 2-cyanoacrylates (6) in the presence of triethylamine in diethyl formamide to give 1-[2-(ethoxy)carbonyl-2-cyano-1-arylvinyl]-2-oxo-1,2-dihydroquinoline-3-carboxylic (7a,d). Cyclization of compound 7 with hydrazine hydrate ethanol inferred the association of 1-[(aryl)(3 amino-5-oxopyrazolidin-4-ylidene)methyl-2-oxo-1,2-dihydroquinol-3-carboxylates (8a,d). Spectroscopic and micro-analytical techniques such as IR, NMR, and elemental analysis were used to validate the structure of the synthesized organic compounds. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested by using the MTT assay on the MCF-7 cell line. When compared to the reference compound Dox, the compounds 7b, 7c, 8a, 8b, and 8c demonstrated strong anticancer activity against the MCF-7 cell line. The anticancer effects of the synthesized compounds 7a–d and 8a–d were tested against the MCF-7 cell line, using MTT assay. The compounds 7b, 7c, 8a, 8b, and 8c showed significant anticancer activity compared to the reference compound Dox against the MCF-7 cell line.

scholarly journals In vitro and in vivo Evaluation of Ibuprofen Nanosuspensions for Enhanced Oral Bioavailability

2021 ◽  
Author(s):  
Mohsen Hedaya ◽  
Farzana Bandarkar ◽  
Aly Nada
Keyword(s):  
Particle Size ◽  
Tween 80 ◽  
Aqueous Solubility ◽  
In Vitro Dissolution ◽  
In Vivo Evaluation ◽  
Poorly Soluble ◽  
Extent Of Absorption ◽  
Better Than

Introduction: The objectives were to prepare, characterize and in vivo evaluate different ibuprofen (IBU) nanosuspensions prepared by ultra-homogenization, after oral administration to rabbits. Methods: The nanosuspensions produced by ultra-homogenization were tested and compared with a marketed IBU suspension for particle size, in vitro dissolution and in vivo absorption. Five groups of rabbits received orally 25 mg/kg of IBU nanosuspension, nanoparticles, unhomogenized suspension, marketed product and untreated suspension. A sixth group received 5 mg/kg IBU intravenously. Serial blood samples were obtained after IBU administration. Results: The formulated nanosuspensions showed significant decrease in particle size. Polyvinyl Pyrrolidone K30 (PP) was found to improve IBU aqueous solubility much better than the other tested polymers. Addition of Tween 80 (TW), in equal amount as PP (IBU: PP:TW, 1:2:2 w/w) resulted in much smaller particle size and better dissolution rate. The Cmax achieved were 14.8±1.64, 11.1±1.37, 9.01±0.761, 7.03±1.38 and 3.23±1.03 μg/ml and the tmax were 36±8.2, 39±8.2, 100±17.3, 112±15 and 105±17 min for the nanosuspension, nanoparticle, unhomogenized suspension, marketed IBU suspension and untreated IBU suspension in water, respectively. Bioavailability of the different formulations relative to the marketed suspension were the highest for nanosuspension> unhomogenized suspension> nanoparticles> untreated IBU suspension. Conclusion: IBU/PP/TW nanosuspensions showed enhanced in vitro dissolution as well as faster rate and higher extent of absorption as indicated from the higher Cmax, shorter tmax and larger AUC. The in vivo data supported the in vitro results. Nanosuspensions prepared by ultra-high-pressure-homogenization technique can be used as a good formulation strategy to enhance the rate and extent of absorption of poorly soluble drugs.

Enrichment of a Nigerian chromite ore for metallurgical application by dense medium flotation and magnetic separation

2019 ◽  
Vol 116 (3) ◽  
pp. 324 ◽  
Author(s):  
Kuranga Ibrahim Ayinla ◽  
Alafara Abdullahi Baba ◽  
Bankim Chandra Tripathy ◽  
Malay Kumar Ghosh ◽  
Rajan Kumar Dwari ◽  
...  
Keyword(s):  
Particle Size ◽  
Magnetic Separation ◽  
Organic Liquid ◽  
Size Fraction ◽  
Dense Medium ◽  
Chromium Alloy ◽  
Magnetic Separator ◽  
Electro Deposition ◽  
Chromite Ore ◽  
Wet Chemical

This study, focused on the beneficiation of a Nigerian complex chromite ore sourced from Tunga-Kaduka, Anka Local Government of Zamfara State, Nigeria, assaying 45.85% Cr2O4 and 54.15% mineral impurities, was enriched concurrently through sink floatation and magnetic separation techniques. The chromite ore initially analyzed to contain silicate impurities was found not suitable for metallurgical purposes. Thus, enrichment was examined through gravity separation studies using organic liquid with different specific gravities at 2.8, 3.3, and 4.0. The separation of chromite ore with lowest particle size fraction was done using Mozley mineral separator followed by the magnetic separation of the sink product by magnetic separator. The results obtained revealed about 77% of the total material containing 300 μm particle size, 52% ˂ 212 μm and 17% below 75 μm. Subsequent analysis of the beneficiated ore was carried out by wet chemical analysis and atomic absorption spectrophotometer. The results showed that Cr2O4 content increased to 78.34% from initial 45.83% with maximum Cr:Fe ratio of 3.2:1, representing 84.27% of chromium metal present in the ore. The enrichment of Cr2O4 obtained in this study could be found metallurgically applicable in the electro-deposition and ferro-chromium alloy production practices.

Up-conversion emission and in vitro cytotoxicity characterization of blue emitting, biocompatible SrTiO3 nanoparticles activated with Tm3+ and Yb3+ ions

RSC Advances ◽  
2016 ◽  
Vol 6 (45) ◽  
pp. 39469-39479 ◽  
Author(s):  
R. Pazik ◽  
A. Zięcina ◽  
B. Poźniak ◽  
M. Malecka ◽  
L. Marciniak ◽  
...  
Keyword(s):  
Particle Size ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Ion Release ◽  
Size Dependent ◽  
U2os Cells

Blue emitting, up-converting NP's of SrTiO3:Tm3+/Yb3+ synthesized using the citric route are biocompatible towards J774.E whereas the cytotoxic effect to U2OS cells is not particle size dependent but most probably is related to Sr2+ ion release.

scholarly journals Apoptosis Induced by Tanshinone IIA and Cryptotanshinone Is Mediated by Distinct JAK/STAT3/5 and SHP1/2 Signaling in Chronic Myeloid Leukemia K562 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Ji Hoon Jung ◽  
Tae-Rin Kwon ◽  
Soo-Jin Jeong ◽  
Eun-Ok Kim ◽  
Eun Jung Sohn ◽  
...  
Keyword(s):  
Chronic Myeloid Leukemia ◽  
Myeloid Leukemia ◽  
Biological Effects ◽  
K562 Cells ◽  
Tanshinone Iia ◽  
Anticancer Activities ◽  
Caspase 9 ◽  
Anticancer Effects ◽  
Induced Apoptosis ◽  
Better Than

Though tanshinone IIA and cryptotanshinone possess a variety of biological effects such as anti-inflammatory, antioxidative, antimetabolic, and anticancer effects, the precise molecular targets or pathways responsible for anticancer activities of tanshinone IIA and cryptotanshinone in chronic myeloid leukemia (CML) still remain unclear. In the present study, we investigated the effect of tanshinone IIA and cryptotanshinone on the Janus activated kinase (JAK)/signal transducer and activator of transcription (STAT) signaling during apoptotic process. We found that both tanshinone IIA and cryptotanshinone induced apoptosis by activation of caspase-9/3 and Sub-G1 accumulation in K562 cells. However, they have the distinct JAK/STAT pathway, in which tanshinone IIA inhibits JAK2/STAT5 signaling, whereas cryptotanshinone targets the JAK2/STAT3. In addition, tanshinone IIA enhanced the expression of both SHP-1 and -2, while cryptotanshinone regulated the expression of only SHP-1. Both tanshinone IIA and cryptotanshinone attenuated the expression of bcl-xL, survivin, and cyclin D1. Furthermore, tanshinone IIA augmented synergy with imatinib, a CML chemotherapeutic drug, better than cryptotanshinone in K562 cells. Overall, our findings suggest that the anticancer activity of tanshinone IIA and cryptotanshinone is mediated by the distinct the JAK/STAT3/5 and SHP1/2 signaling, and tanshinone IIA has the potential for combination therapy with imatinib in K562 CML cells.

Particle size- and concentration-dependent separation of magnetic nanoparticles

2017 ◽  
Vol 427 ◽  
pp. 320-324 ◽  
Author(s):  
Kerstin Witte ◽  
Knut Müller ◽  
Cordula Grüttner ◽  
Fritz Westphal ◽  
Christer Johansson
Keyword(s):  
Particle Size ◽  
Magnetic Nanoparticles

Particle size dependent hysteresis loss in La0.7Ce0.3Fe11.6Si1.4C0.2 first‐order systems

2012 ◽  
Vol 100 (7) ◽  
pp. 072403 ◽  
Author(s):  
F. X. Hu ◽  
L. Chen ◽  
J. Wang ◽  
L. F. Bao ◽  
J. R. Sun ◽  
...  
Keyword(s):  
Particle Size ◽  
Hysteresis Loss ◽  
First Order ◽  
Size Dependent

scholarly journals Dehumidified Air-Assisted Spray-Drying of Cloudy Beetroot Juice at Low Temperature

2021 ◽  
Vol 11 (14) ◽  
pp. 6578
Author(s):  
Aleksandra Jedlińska ◽  
Alicja Barańska ◽  
Dorota Witrowa-Rajchert ◽  
Ewa Ostrowska-Ligęza ◽  
Katarzyna Samborska
Keyword(s):  
Particle Size ◽  
Physicochemical Properties ◽  
Spray Drying ◽  
Low Temperatures ◽  
Beetroot Juice ◽  
Particle Size And Morphology ◽  
Size And Morphology ◽  
Tapped Density ◽  
Lower Loss ◽  
Better Than

This paper discusses the physicochemical properties of powders obtained by spray drying of cloudy beetroot juice, using dehumidified air in variants with or without carriers. The inlet air temperature was 130 °C or 90 °C, and the addition of the carriers was at a ratio of juice to carrier solids of 3:2. In the obtained powders, the following physicochemical properties were determined: water content and water activity, apparent density, loose and tapped density, porosity, flowability, particle size and morphology, and the content and retention of betalains. It was possible to dry cloudy beetroot juice without the use of carriers at low temperatures (90 or 130 °C). The 100% beetroot powders were characterized by satisfactory physicochemical properties, often better than those with carriers (including lower hygroscopicity and higher color saturation and yield). A lower loss of betalains was found for the powders with the addition of carriers. The best process yields were obtained for the powder without carriers at 130 °C and 90 °C.

Sign in / Sign up

Export Citation Format

Share Document