Up-conversion emission and in vitro cytotoxicity characterization of blue emitting, biocompatible SrTiO3 nanoparticles activated with Tm3+ and Yb3+ ions

RSC Advances ◽  
2016 ◽  
Vol 6 (45) ◽  
pp. 39469-39479 ◽  
Author(s):  
R. Pazik ◽  
A. Zięcina ◽  
B. Poźniak ◽  
M. Malecka ◽  
L. Marciniak ◽  
...  
Keyword(s):  
Particle Size ◽  
Cytotoxic Effect ◽  
In Vitro Cytotoxicity ◽  
Ion Release ◽  
Size Dependent ◽  
U2os Cells

Blue emitting, up-converting NP's of SrTiO3:Tm3+/Yb3+ synthesized using the citric route are biocompatible towards J774.E whereas the cytotoxic effect to U2OS cells is not particle size dependent but most probably is related to Sr2+ ion release.

scholarly journals In vitro cytotoxicity of Aspilia pluriseta Schweinf. extract fractions

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Sospeter N. Njeru ◽  
Jackson M. Muema
Keyword(s):  
Biological Activities ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Root Extract ◽  
Lack Of Information ◽  
Information Gap ◽  
Diphenyltetrazolium Bromide ◽  
Potential Use

Abstract Objectives We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on its cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemical characterization of A. pluriseta root extracts, here we report on cytotoxicity of tested solvent fractions. We evaluated the potential cytotoxicity of these root extract fractions on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results We show that all solvent extract fractions (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions’ earlier reported bioactivities are within the acceptable cytotoxicity and selective index limits. This finding scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.

Chitosan nanoparticles for tamoxifen delivery and cytotoxicity to MCF-7 and Vero cells

2011 ◽  
Vol 83 (11) ◽  
pp. 2027-2040 ◽  
Author(s):  
Neralakere Ramanna Ravikumara ◽  
Basavaraj Madhusudhan
Keyword(s):  
Particle Size ◽  
Chitosan Nanoparticles ◽  
Vero Cells ◽  
In Vitro Cytotoxicity ◽  
Correlation Spectroscopy ◽  
Scanning Calorimetry ◽  
Ζ Potential ◽  
Human Red Blood Cells ◽  
Tamoxifen Citrate

In this study, tamoxifen citrate-loaded chitosan nanoparticles (tamoxcL-ChtNPs) and tamoxifen citrate-free chitosan nanoparticles (tamoxcF-ChtNPs) were prepared by an ionic gelation (IG) method. The physicochemical properties of the nanoparticles were analyzed for particle size, zeta (ζ) potential, and other characteristics using photon correlation spectroscopy (PCS), zeta phase analysis light scattering (PALS), scanning electron microscopy (SEM), Fourier transform infrared (FTIR), and differential scanning calorimetry (DSC). The variation in particle size was assessed by changing the concentration of chitosan, pentasodium tripolyphosphate (TPP), and the pH of the solution. The optimized tamoxcL-ChtNPs showed mean diameter of 187 nm, polydispersity of 0.125, and ζ-potential of +19.1 mV. The encapsulation efficiency (EE) of tamoxifen citrate (tamoxc) increased at higher concentrations, and release of tamoxc from the chitosan matrix displayed controlled biphasic behavior. Those tamoxcL-ChtNPs tested for chemosensitivity showed dose- and time-dependent antiproliferative activity of tamoxc. Further, tamoxcL-ChtNPs were found to be hemocompatible with human red blood cells (RBCs) and safe by in vitro cytotoxicity tests, suggesting that they offer promise as drug delivery systems in therapy.

scholarly journals Preparation and characterization of domperidone nanoparticles for dissolution improvement

2018 ◽  
Vol 27 (1) ◽  
pp. 39-52
Author(s):  
Mohammed Sabar Al-lami ◽  
Malath H. Oudah ◽  
Firas A. Rahi
Keyword(s):  
Particle Size ◽  
Average Particle Size ◽  
In Vitro Release ◽  
Methyl Cellulose ◽  
Precipitation Method ◽  
Average Particle ◽  
Antisolvent Precipitation ◽  
Stirring Time

This study was carried out to prepare and characterize domperidone nanoparticles to enhance solubility and the release rate. Domperidone is practically insoluble in water and has low and an erratic bioavailability range from 13%-17%. The domperidone nanoparticles were prepared by solvent/antisolvent precipitation method at different polymer:drug ratios of 1:1 and 2:1 using different polymers and grades of poly vinyl pyrolidone, hydroxy propyl methyl cellulose and sodium carboxymethyl cellulose as stabilizers. The effect of polymer type, ratio of polymer:drug, solvent:antisolvent ratio, stirring rate and stirring time on the particle size, were investigated and found to have a significant (p? 0.05) effect on particle size. The best formula was obtained with lowest average particle size of 84.05. This formula was studied for compatibility by FTIR and DSC, surface morphology by FESEM and crystalline state by XRPD. Then domperidone nanoparticles were formulated into a simple capsule dosage form in order to study of the in vitro release of drug from nanoparticles in comparison raw drug and mixture of polymer:drug ratios of 2:1. The release of domperidone from best formula was highly improved with a significant (p? 0.05) increase.

scholarly journals Synthesis, Characterization and in vitro Antimicrobial, Anticancer Activity of Random Copolyester using Orcinol

2021 ◽  
Vol 33 (9) ◽  
pp. 2119-2126
Author(s):  
T. Sivaramakrishnan ◽  
G. Elango
Keyword(s):  
In Vitro Cytotoxicity ◽  
Diffusion Method ◽  
Dsc Studies ◽  
Repeat Units ◽  
Nmr Techniques ◽  
Crystalline Nature ◽  
Uv Visible ◽  
Tga And Dsc

In present study, a novel random copolyester i.e. poly(3-oxy-5-methylphenyl-4-oxynaphthal-1- yl)isophthalate (PONI) was synthesized through solution polycondensation methodology involving orcinol, 1,4-naphthalene diol and isophthaloyl chloride and its solubility in common solvents along with viscosity measurements were performed. The structure of the repeat units in the polyester chain were ascertained based on the spectral characterization of UV-visible, FTIR, 1H & 13C NMR techniques. Thermal analysis of the copolyester were performed by TGA and DSC studies. The activation energy for thermal decomposition of polyester was estimated by well-known kinetic methods. The surface morphology and crystalline nature of the polymer were explored by employing SEM and WAXD methods. The antimicrobial efficacy of copolyester was assessed through well-diffusion method using a Gram-positive and a Gram-negative bacteria. The in vitro cytotoxicity of the polyester prepared was verified against HeLa cell line using MTT assay.

scholarly journals Preparation and Characterization of Etodolac as a Topical Nanosponges Hydrogel

2019 ◽  
Vol 28 (1) ◽  
pp. 64-74
Author(s):  
Maysam M. Abass ◽  
Nawal A. Rajab
Keyword(s):  
Particle Size ◽  
In Vitro Release ◽  
Diffusion Method ◽  
Internal Phase ◽  
Stirring Time ◽  
Vitro Release ◽  
Emulsion Solvent Diffusion Method ◽  
Polymer Ratio

Nanosponges (NS) of etodolac(ETO) was prepared using the emulsion solvent diffusion method ; the effects of drug: polymer ratio, the effect of level concentration of internal phase and stirring time and other variables that effect on the physical characteristics of NS were investigated and characterized, The selected formula was lyophilized then incorporated into hydrogel ; which also evaluated .The results show that the formulation that contain Drug: PVA:EC in ratio 1:3:2 is the best with smallest particle size 40.2±0.098 with polydispersibility0.005 and in vitro release 97.6±0.11%, , ETO NS Carbopol hydrogel produced a significant(p<0.05) improvement of the in vitro release than pure ETO hydrogel.

scholarly journals In Vitro Cytotoxicity of the Synthesized Gallic Acid Derivatives (N-Alkyl Gallamide) Against Breast MCF-7 Cancer Cells

2018 ◽  
Vol 34 (5) ◽  
pp. 2268-2272
Author(s):  
Maurin Marcelia ◽  
Ade Arsianti ◽  
Jilly Octaria Tagore Chan ◽  
Stevano Julio Wijoyo ◽  
Fadilah Fadilah ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gallic Acid ◽  
Cytotoxic Effect ◽  
Linear Regression Analysis ◽  
Cancer Cell Line ◽  
In Vitro Cytotoxicity ◽  
Tert Butyl ◽  
Ic50 Value ◽  
Mcf 7

Gallic acid is a phenolic compound distributed in plants and fruits which has been reported to have cytotoxic effect on MCF-7 breast cancer cell line. In this research, we investigated in vitro cytotoxic effect of six synthesized compounds of gallic acid derivatives (N-alkyl gallamide), namely N-methyl gallamide (2); N-ethyl gallamide (3); N-butyl gallamide (4); N-sec-butyl gallamide (5); N-tert-butyl gallamide (6) and N-hexyl gallamide (7) against breast MCF-7 cells by MTT assay. Linear regression analysis is utilized to analyze data to regenerate IC50 value. The results will be compared with gallic acid as an original compound and doxorubicin as a positive control.Among six synthesized compounds, N-tert-butyl gallamide (6) with IC50 value of 2.1 µg/mL, and N-hexyl gallamide (7) with IC50 value of 3.5µg/mL,showed the stronger cytotoxicity against breast MCF-7 cells compared to gallic acid and doxorubicin. Thus,N-tert-butyl gallamide (6) and N-hexyl gallamide (7) are potential to be further developed as a promising anti-breast cancer agents.

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