Expression of Pentraxin 3 and Thrombospondin 1 in Gingival Crevicular Fluid during Wound Healing after Gingivectomy in Postorthodontic Patients

Background. Wound healing is a tissue repair process after an injury, and two of its main components are inflammation and angiogenesis, in which course a cascade of mediators is involved. The aim of this research was to evaluate the involvement of Pentraxin 3 and Thrombospondin 1 in wound healing after periodontal surgery (gingivectomy) for gingival overgrowth during orthodontic treatment with or without magnification devices, by assessing their levels in GCF.Methods. From 19 patients with gingival overgrowth as a result of fixed orthodontic treatment, the overgrown gingiva was removed by gingivectomy, from one half of the mandibular arch without magnification and from the other under magnification. Pentraxin 3 and Thrombospondin 1 were determined from gingival crevicular fluid by ELISA tests.Results. Statistically significant differences (p<0.05) and correlations between levels of the two biomarkers were analyzed. Statistically significant differences were established between levels of the two biomarkers at different time points, with significant positive correlation at the point of 24 hours.Conclusions. Within the limitations of this study, the results seem to sustain the involvement of Pentraxin 3 and Thrombospondin 1 in the processes of inflammation and angiogenesis in wound healing of patients with postorthodontic gingivectomy. The dynamics of Pentraxin 3 and Thrombospondin 1 levels could suggest a reduced inflammation and a faster angiogenesis using microsurgery.

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Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel–Titanium Coil Spring and Active Tieback

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-2112 ◽

2017 ◽

Vol 18 (8) ◽

pp. 710-713

Author(s):

Sukhpreet Mangat ◽

Rakesh Thukral ◽

Anvesha Ganguly ◽

Harleen Bali ◽

Shekhar Grover

Keyword(s):

Orthodontic Treatment ◽

Gingival Crevicular Fluid ◽

Nickel Titanium ◽

Enzyme Linked Immunosorbent Assay ◽

Pentraxin 3 ◽

Coil Spring ◽

Time Intervals ◽

Canine Retraction ◽

Significant Difference ◽

Crevicular Fluid

ABSTRACT Aim Orthodontic treatment is routinely carried out in patients with the purpose of correcting various forms of dental malocclusions. Retraction of the canines can be achieved either individually or along with incisor. Pentraxin-3 (PTX-3) is regarded as the true independent indicator of disease activity. Hence, we undertook the present study to assess and compare the level of PTX-3 in patients undergoing canine retraction with active tieback and Nickel–Titanium (NiTi) coil spring. Materials and methods The present study included assessment of 25 patients that underwent canine retraction as a part of fixed orthodontic treatment. In the maxillary right and left quadrant, active tieback and NiTi coil spring were used respectively. Gingival crevicular fluid samples were collected 1 hour prior to the commencement of orthodontic canine retraction procedure followed by collection at following time intervals: 1 hour, 1, 7, and 14 days after the start of canine retraction procedure. Enzyme-linked immunosorbent assay kit was used for analysis of the samples as per manufacturer's instructions. All the data were recorded and compiled. All the results were analyzed by Statistical Package for the Social Sciences software. Results Nonsignificant difference was observed in the values of PTX-3 at baseline and 1 hour. While comparing the mean PTX-3 values between different time intervals in both the study groups, significant difference was observed. Conclusion In comparison to the active tieback, NiTi coil exhibited faster space closure rate. Clinical significance In patients undergoing orthodontic treatment, PTX-3 is associated with periodontal remodeling under the effect of orthodontic forces. How to cite this article Thukral R, Mangat S, Ganguly A, Agarkar SS, Bali H, Grover S. Pentraxin-3 Levels in Gingival Crevicular Fluid during Canine Retraction with Nickel–Titanium Coil Spring and Active Tieback. J Contemp Dent Pract 2017; 18(8):710-713.

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STUDY OF GINGIVAL CREVICULAR FLUID AS A PREDICTOR OF THE DURATION OF THE PERIOD OF RETENTION AFTER MAJOR ORTHODONTIC TREATMENT

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2020-22-8-6-10 ◽

2020 ◽

Vol 22 (8) ◽

pp. 6-10

Author(s):

M.Yu. Kuznetsova ◽

A.V. Sevbitov ◽

V.V. Platonova ◽

S.N. Mironov ◽

I.I. Kuznetsov

Keyword(s):

Orthodontic Treatment ◽

Gingival Crevicular Fluid ◽

Crevicular Fluid

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Gingival Crevicular Fluid Characterization During Orthodontic Treatment

Case Medical Research ◽

10.31525/ct1-nct04226469 ◽

2020 ◽

Author(s):

Keyword(s):

Orthodontic Treatment ◽

Gingival Crevicular Fluid ◽

Fluid Characterization ◽

Crevicular Fluid

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ESTIMATION OF PENTRAXIN-3 AND TUMOR NECROSIS ALPHA LEVELS IN GINGIVAL CREVICULAR FLUID OF CHRONIC PERIODONTITIS PATIENTS

Egyptian Dental Journal ◽

10.21608/edj.2017.75005 ◽

2017 ◽

Vol 63 (1) ◽

pp. 555-563

Author(s):

Ebtehal Hamdy ◽

Ibtesam Afifi

Keyword(s):

Chronic Periodontitis ◽

Tumor Necrosis ◽

Gingival Crevicular Fluid ◽

Pentraxin 3 ◽

Crevicular Fluid

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Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

Applied Sciences ◽

10.3390/app11020521 ◽

2021 ◽

Vol 11 (2) ◽

pp. 521

Author(s):

Simina Chelărescu ◽

Petra Șurlin ◽

Mioara Decusară ◽

Mădălina Oprică ◽

Eugen Bud ◽

...

Keyword(s):

Young Adults ◽

Orthodontic Treatment ◽

Tooth Movement ◽

Gingival Crevicular Fluid ◽

Early Stage ◽

Statistical Tests ◽

Orthodontic Tooth Movement ◽

Adolescents And Young Adults ◽

Periodontal Tissues ◽

Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

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