scholarly journals Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Carola Cavallo ◽  
Alice Roffi ◽  
Brunella Grigolo ◽  
Erminia Mariani ◽  
Loredana Pratelli ◽  
...  
Keyword(s):  
Collagen Type ◽  
Platelet Rich Plasma ◽  
Low Cost ◽  
Immediate Release ◽  
Collagen Type I ◽  
Bioactive Molecules ◽  
Release Kinetic ◽  
Type I ◽  
Physical Form ◽  
High Concentrations

Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2+ thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α. PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed. Collagen type I produced an overall lower GF release. Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF-β1that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours. CaCl2induced a progressive release of GFs from 15 minutes and increasing up to 24 hours. The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.

Relation of High Concentrations of Plasma Carboxy-Terminal Telopeptide of Collagen Type I With Outcome in Acute Myocardial Infarction

2009 ◽  
Vol 104 (7) ◽  
pp. 904-909 ◽  
Author(s):  
Olivier Barthélémy ◽  
Farzin Beygui ◽  
Eric Vicaut ◽  
Stephanie Rouanet ◽  
Eric Van Belle ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Collagen Type ◽  
Collagen Type I ◽  
Type I ◽  
High Concentrations ◽  
Carboxy Terminal

scholarly journals Fibrin coating of polymer vascular graft does not reduce its thromboresistance

2020 ◽  
Vol 5 (2) ◽  
pp. 22-29
Author(s):  
E. A. Velikanova ◽  
T. V. Glushkova ◽  
T. N. Akentyeva ◽  
V. G. Matveeva ◽  
M. Yu. Khanova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Platelet Aggregation ◽  
Vascular Graft ◽  
Collagen Type ◽  
Platelet Rich Plasma ◽  
Small Diameter ◽  
Collagen Type I ◽  
Type I ◽  
Nuclear Staining ◽  
Human Coronary Artery

Aim. To evaluate biocompatibility along with adhesion and aggregation of platelets on the surface of uncoated and fibrin-coated poly(3-hydroxybutyrate- co-3-hydroxyvalerate)/poly(ε-caprolactone) (PHBV/PCL) small-diameter vascular grafts.Materials and Methods. 4 mm diameter grafts were fabricated by electrospinning from PHBV/ PCL (1:2) blend dissolved in 1,1,1,3,3,3-hexafluoro- 2-propanol. Inner wall of the grafts was produced using co-electrospinning of the polymer blend and collagen type I (5 mg/mL) from two different syringes. Fibrinogen was obtained from the blood of healthy donors by a cryoprecipitation procedure. Sterile polymer scaffolds were impregnated into a fibrinogen solution and immersed in a thrombin/calcium chloride blend for polymerization. To assess the biocompatibility of the grafts, primary human coronary artery endothelial cells were seeded on the luminal surface and counted under a fluorescence microscope after nuclear staining. Hemocompatibility was tested by incubation of the grafts with human platelet-rich plasma. Platelet aggregation was assessed using a platelet aggregation analyser. Surface morphology, platelet adhesion and activation were evaluated by scanning electron microscopy.Results. Fibrin coating promoted cell adhesion and proliferation and improved the graft biocompatibility as evidenced by a higher number of endothelial cells. Fibrin coating did not increase platelet aggregation, adhesion, and activation and therefore did not reduce the thromboresistance of vascular graft.Conclusion. The fibrin modification of polymer grafts from PHBV/PCL blend and collagen type I improves the surface biocompatibility and does not reduce its thromboresistance.

scholarly journals Redifferentiation of Articular Chondrocytes by Hyperacute Serum and Platelet Rich Plasma in Collagen Type I Hydrogels

2019 ◽  
Vol 20 (2) ◽  
pp. 316 ◽  
Author(s):  
Vivek Jeyakumar ◽  
Eugenia Niculescu-Morzsa ◽  
Christoph Bauer ◽  
Zsombor Lacza ◽  
Stefan Nehrer
Keyword(s):  
Collagen Type ◽  
Articular Chondrocytes ◽  
Platelet Rich Plasma ◽  
Collagen Type I ◽  
Cartilage Defects ◽  
Type I ◽  
New Approach ◽  
Sgag Content ◽  
Col2a1 Expression

Matrix-assisted autologous chondrocyte transplantation (MACT) for focal articular cartilage defects often fails to produce adequate cartilage-specific extracellular matrix in vitro and upon transplantation results in fibrocartilage due to dedifferentiation during cell expansion. This study aimed to redifferentiate the chondrocytes through supplementation of blood-products, such as hyperacute serum (HAS) and platelet-rich plasma (PRP) in vitro. Dedifferentiated monolayer chondrocytes embedded onto collagen type I hydrogels were redifferentiated through supplementation of 10% HAS or 10% PRP for 14 days in vitro under normoxia (20% O2) and hypoxia (4% O2). Cell proliferation was increased by supplementing HAS for 14 days (p < 0.05) or by interchanging from HAS to PRP during Days 7–14 (p < 0.05). Sulfated glycosaminoglycan (sGAG) content was deposited under both HAS, and PRP for 14 days and an interchange during Days 7–14 depleted the sGAG content to a certain extent. PRP enhanced the gene expression of anabolic markers COL2A1 and SOX9 (p < 0.05), whereas HAS enhanced COL1A1 production. An interchange led to reduction of COL1A1 and COL2A1 expression marked by increased MMP13 expression (p < 0.05). Chondrocytes secreted less IL-6 and more PDGF-BB under PRP for 14 days (p < 0.0.5). Hypoxia enhanced TGF-β1 and BMP-2 release in both HAS and PRP. Our study demonstrates a new approach for chondrocyte redifferentiation.

scholarly journals Proliferation, Migration, and ECM Formation Potential of Human Annulus Fibrosus Cells Is Independent of Degeneration Status

Cartilage ◽  
2018 ◽  
Vol 11 (2) ◽  
pp. 192-202 ◽  
Author(s):  
Sylvia Hondke ◽  
Mario Cabraja ◽  
Jan Philipp Krüger ◽  
Stefan Stich ◽  
Tony Hartwig ◽  
...  
Keyword(s):  
Gene Expression ◽  
Annulus Fibrosus ◽  
Collagen Type ◽  
Transforming Growth Factor ◽  
Platelet Rich Plasma ◽  
Collagen Type I ◽  
Type I ◽  
Type Ii ◽  
Cell Source ◽  
Cell Groups

Objective The objective was to evaluate the proliferating, migratory and extracellular matrix (ECM) forming potential of annulus fibrosus cells derived from early (edAFC) or advanced (adAFC) degenerative tissue and their usability as a possible cell source for regenerative approaches for AF closure. Design EdAFC ( n = 5 Pfirrman score of 2-3) and adAFC (n = 5 Pfirrman score of 4-5) were isolated from tissue of patients undergoing spine stabilizing surgery. Cell migration on stimulation with human serum (HS), platelet-rich plasma (PRP), and transforming growth factor β-3 (TGFB3) was assessed by migration assay and proliferation was assessed on stimulation with HS. Induction of ECM synthesis was evaluated by gene expression analysis of AF-related genes in three-dimensional scaffold cultures that have been stimulated with 5% PRP or 10 ng/mL TGFB3 and histologically by collagen type I, type II, alcian blue, and safranin-O staining. Results EdAFC and adAFC were significantly attracted by 10% HS and 5% PRP. Additionally, both cell groups proliferated under stimulation with HS. Stimulation with 10 ng/mL TGFB3 showed significant induction of gene expression of collagen type II and aggrecan, while 5% PRP decreased the expression of collagen type I. Both cell groups showed formation of AF-like ECM after stimulation with TGFB3, whereas stimulation with PRP did not. Conclusions Our study demonstrated that AF cells retain their potential for proliferation, migration, and ECM formation independent of the degeneration status of the tissue. Proliferation, migration, and ECM synthesis of the endogenous AF cells can be supported by different supplements. Hence, endogenous AF cells might be a suitable cell source for a regenerative repair approaches.

scholarly journals Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells (rBMMSCs) in Collagen Type I Containing Platelet-Rich Plasma for Osteoarthritis Treatment in Rat Model

2021 ◽  
Author(s):  
somayeh Ebrahimi-barough ◽  
Md Shahidul Islam ◽  
Mamun Al Mahtab ◽  
Sadegh Shirian ◽  
Hamid Reza Aghayan ◽  
...  
Keyword(s):  
Knee Joint ◽  
Rat Model ◽  
Collagen Type ◽  
Platelet Rich Plasma ◽  
Cartilage Damage ◽  
Collagen Type I ◽  
Joint Disease ◽  
Type I ◽  
Regenerative Effect

Abstract Osteoarthritis (OA) is the most common form of degenerative joint disease, affecting more than 25% of the adult though prevalent in the elderly population. Most of the current therapeutic modalities aim at symptomatic treatment and lingering the disease progression. In recent years, regenerative medicine such as stem cell transplantation and tissue engineering has been suggested as a potential curative intervention for OA. The objective of current study was to assess the safety and efficacy of an injectable tissue-engineered construct composed of BMMSCs, PRP, and Collagen type I in rat model of OA.To produce collagen type I, PRP and BMMSCs, male Wistar rats were ethically euthanized. After expansion and characterization of rat BMMSCs (rBMMSCs), tissue-engineered construct was formed by combination of appropriate amount of collagen type I, PRP and rBMMSCs. In vitro studies were conducted to evaluate the effect of PRP on chondrogenic differentiation capacity of encapsulated cells. Then tissue-engineered construct was injected in knee joint of rat model of OA (24 rat in 4 groups:OA, OA+MSC, ‎OA+Collagen+MSC+PRP, OA+MSC+Collagen).After 6 weeks, the animals were euthanized and knee joint histopathology examinations were performed to evaluate the effect of each treatment on OA.Tissue-engineered construct was successfully manufactured and in vitro assays demonstrated that relevant chondrogenic genes and proteins expression were higher in PRP group than the others. Histopathological findings of the knee joint samples showed favorable regenerative effect of rBMMSCs+PRP+Collagengroup comparing to others.In this study, we introduced an injectable tissue-engineered product composed of rBMMSCs+PRP+Collagenwith potential regenerative effect on cartilage damage caused by OA.

scholarly journals Repair of rat cranial bone defect by using amniotic fluid-derived mesenchymal stem cells in polycaprolactone fibrous scaffolds and platelet-rich plasma

Bioimpacts ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 209-217
Author(s):  
Zeinab Ghaffarinovin ◽  
Omid Soltaninia ◽  
Yousef Mortazavi ◽  
Abdolreza Esmaeilzadeh ◽  
Samad Nadri
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Collagen Type ◽  
Platelet Rich Plasma ◽  
Bone Defects ◽  
Collagen Type I ◽  
Cranial Bone ◽  
Type I ◽  
Simultaneous Application

Introduction: Tissue regenerative medicine strategies, as a promising alternative has become of major interest to the reconstruction of critical size bone defects. This study evaluated the effects of the simultaneous application of polycaprolactone (PCL), amniotic fluid mesenchymal stem cells (AF-MSCs) and platelet-rich plasma (PRP) on the repair of rat cranial bone defects. Methods: The AF-MSCs were isolated at the end of the second week of pregnancy in rats. PRP obtained from rat blood and the random PCL fibrous scaffolds were prepared using the electrospinning method. Circular full thickness (5 mm) bone defects were developed on both sides of the parietal bones (animal number=24) and the scaffolds containing AF-MSCs and PRP were implanted in the right lesions. Thereafter, after eight weeks the histological and immunohistochemistry studies were performed to evaluate the bone formation and collagen type I expression. Results: The spindle-shaped mesenchymal stem cells were isolated and the electron microscope images indicated the preparation of a random PCL scaffold. Immunohistochemical findings showed that collagen type I was expressed by AF-MSCs cultured on the scaffold. The results of hematoxylin and eosin (H & E) staining indicated the formation of blood vessels in the presence of PRP. Additionally, immunofluorescence findings suggested that PRP had a positive effect on collagen type I expression. Conclusion: The simultaneous application of fibrous scaffold + AF-MSCs + PRP has positive effects on bone regeneration. This study showed that PRP can affect the formation of new blood vessels in the scaffold transplanted in the bone defect.

scholarly journals Vascular Tissue Engineering: Effects of Integrating Collagen into a PCL Based Nanofiber Material

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Ulf Bertram ◽  
Dominik Steiner ◽  
Benjamin Poppitz ◽  
Dirk Dippold ◽  
Katrin Köhn ◽  
...  
Keyword(s):  
Collagen Type ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Collagen Type I ◽  
Collagen I ◽  
Type I ◽  
Native Proteins ◽  
Morphological Aspects ◽  
High Concentrations

The engineering of vascular grafts is a growing field in regenerative medicine. Although numerous attempts have been made, the current vascular grafts made of polyurethane (PU), Dacron®, or Teflon® still display unsatisfying results. Electrospinning of biopolymers and native proteins has been in the focus of research to imitate the extracellular matrix (ECM) of vessels to produce a small caliber, off-the-shelf tissue engineered vascular graft (TEVG) as a substitute for poorly performing PU, Dacron, or Teflon prostheses. Blended poly-ε-caprolactone (PCL)/collagen grafts have shown promising results regarding biomechanical and cell supporting features. In order to find a suitable PCL/collagen blend, we fabricated plane electrospun PCL scaffolds using various collagen type I concentrations ranging from 5% to 75%. We analyzed biocompatibility and morphological aspectsin vitro. Our results show beneficial features of collagen I integration regarding cell viability and functionality, but also adverse effects like the loss of a confluent monolayer at high concentrations of collagen. Furthermore, electrospun PCL scaffolds containing 25% collagen I seem to be ideal for engineering vascular grafts.

scholarly journals Proinflammatory and Anabolic Gene Expression Effects of Platelet-Rich Gel Supernatants on Equine Synovial Membrane Explants Challenged with Lipopolysaccharide

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Jorge U. Carmona ◽  
Diana L. Ríos ◽  
Catalina López ◽  
María E. Álvarez ◽  
Jorge E. Pérez
Keyword(s):  
Gene Expression ◽  
Synovial Membrane ◽  
Matrix Protein ◽  
Collagen Type ◽  
Platelet Rich Plasma ◽  
Rna Extraction ◽  
Collagen Type I ◽  
Type I ◽  
Quantitative Expression ◽  
Anti Inflammatory

Platelet-rich plasma (PRP) preparations are used in horses with osteoarthritis (OA). However, some controversies remain regarding the ideal concentration of platelets and leukocytes to produce an adequate anti-inflammatory and anabolic response in the synovial membrane. The aims of this study were to study the influence of leukoconcentrated platelet-rich gel (Lc-PRG) and leukoreduced platelet-rich gel (Lr-PRG) supernatants on the quantitative expression of some proinflammatory and anabolic genes in equine synovial membrane explants (SMEs) challenged with lipopolysaccharide (LPS). SMEs from six horses were cultured over 96 h. Then, SMEs were harvested for RNA extraction and quantitative gene expression analysis by RT-qPCR for nuclear factor kappa B (NFκB), matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4), collagen type I alpha 1 (COL1A1), collagen type II alpha 1 (COL2A1), and cartilage oligomeric matrix protein (COMP). The 25% and 50% Lc-PRG supernatants led to downregulation of NFκB, MMP-13, ADAMTS-4, COL1A1, COL2A1, and COMP in SMEs. Lr-PRG supernatants (particularly at the 50% concentration) induced downregulation of NFκB, MMP-13, ADAMTS-4, and COL1A1 and upregulation of COL2A1 and COMP. Lr-PRG supernatants should be used for the treatment of inflammatory arthropathies in horses because they have anti-inflammatory and anabolic effects in the synovial membrane.

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