Allium stipitatumExtract Exhibits In Vivo Antibacterial Activity against Methicillin-ResistantStaphylococcus aureusand Accelerates Burn Wound Healing in a Full-Thickness Murine Burn Model

The in vivo antibacterial and burn wound healing potency of Persian shallot bulbs (Allium stipitatum) were explored in a mice burn model infected with methicillin-resistantStaphylococcus aureus(MRSA). Hexane (ASHE) and dichloromethane (ASDE) extracts were tested. Female BALB/c mice were inflicted with third-degree thermal injury followed by infection with MRSA. ASHE and ASDE formulated with simple ointment base (SOB) at concentrations of 1%, 2%, and 5% (w/w) were topically applied to burn wounds twice a day for 20 days. Silver sulfadiazine (1%) served as drug positive control. Microbiological analysis was carried out on 1, 2, 3, 4, and 5 days postwounding (dpw) and histopathological analysis at the end of the experiment (20 dpw). Both ointments demonstrated strong antibacterial activity with complete elimination of MRSA at 48–72 h after infection. The rate of wound contraction was higher (95–100%) in mice groups treated with ASHE and ASDE ointments after 15 dpw. Histological analysis revealed significant increase (p<0.05) in epithelialization and collagenation in treated groups. The ASHE and ASDE were found to be relatively noncytotoxic and safe to Vero cell line (383.4 μg mL−1; 390.6 μg mL−1), suggesting the extracts as safe topical antibacterial as well as promising alternatives in managing thermal injuries.

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Chitosan from Lucilia cuprina (Diptera: Calliphoridae) enhances sensitization to insulin treatment in a diabetic burn mice of wound healing

Swedish Journal of BioScience Research ◽

10.51136/sjbsr.2020.1.15 ◽

2020 ◽

Vol 1 (1) ◽

pp. 1-15

Author(s):

Lamia M. El-Samad ◽

◽

Azza A. Attia ◽

Basant A. Bakr ◽

◽

...

Keyword(s):

Wound Healing ◽

Wound Closure ◽

Lucilia Cuprina ◽

Diabetic Mice ◽

Burn Wound ◽

Time Intervals ◽

Burn Wound Healing ◽

High Degree

Chitosan is recognized as a multipurpose biomaterial because of its low allergenicity, non-toxicity, biodegradability and biocompatibility. The present study was designed to estimate the role of chitosan derived from Lucilia cuprina on burn healing in diabetic mice; using histopathological and microbiological studies at different time intervals. Chitosan was prepared from L. cuprina with high molecular weight (MW) and high degree of deacetylation (DD) to evaluate its burn wound healing potential; skin burn closure assessment, histological and microbiological studies in vivo in male diabetic mice. Chitosan topical treatment was superior in wound closure acceleration; mainly in insulin injected group at all the time intervals. Additionally, earlier epidermal remodelling with mature and intense collagen deposition was encountered in all chitosan treated animals as well as non-diabetic burned animals. There was a significant delay in hair growth and poor epidermal remodelling with impairment of wound closure in diabetic groups. Moreover, chitosan treated groups assert the chitosan antibacterial effects with protecting the burn against contamination that hinders healing especially in this diabetic condition. Further researches needed to interpret effects of possible synergistic combination therapy.

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Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

Folia Morphologica ◽

10.5603/fm.a2019.0038 ◽

2019 ◽

Vol 78 (4) ◽

pp. 798-808 ◽

Cited By ~ 1

Author(s):

R. A. Imam ◽

A. A.-E. Rizk

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Burn Wound ◽

Burn Wound Healing

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Development and characterization of silk films for burn wound healing

Research Journal of Textile and Apparel ◽

10.1108/rjta-11-2019-0056 ◽

2020 ◽

Vol 24 (2) ◽

pp. 131-146

Author(s):

Sukumar Nachiappan ◽

Lami Amanuel ◽

Tewodros Agazie ◽

Seyoum Bihonegn

Keyword(s):

Wound Healing ◽

Research Work ◽

Polymeric Materials ◽

Study Data ◽

Burn Wound ◽

Content Type ◽

Burn Wound Healing ◽

Biomedical Device ◽

Silk Films

Purpose Wound healing is a dynamic process that relies on coordinated signaling molecules to succeed. Silk has proven to be a promising biomaterial for the development of a novel product. The purpose of the study is development of silk films, augmented functionality can be provided to silk by means of loading honey and recombinant human epidermal growth factor (rhEGF). Design/methodology/approach In this research work, the authors set out to explore possibilities of silk-based biomedical device development with particular attention to different fabrication strategies that can be leveraged for this purpose. They have produced a novel silk-based drug delivery material, in the form of silk films. Scanning electronic microscope was used to observe the morphology and the highly specific surface area. The structure was studied by Fourier-transform infrared spectroscopy. This methodology is accomplished using in vivo study data using Wister albonia rats. Findings The developed films also provided a significant higher healing rate in vivo, with well-formed epidermis with faster granulation tissue formation when compared to the controls. Biodegradable polymeric materials based on blending aqueous dispersions of natural polymer sodium alginate, Chitosan and rhEGF complex, which allow controlled antiseptic release, are presented. Originality/value These results suggest that silk-based controlled release of Chitosan-rhEGF may serve as a new therapy to accelerate healing of burn wounds.

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Injectable Nanocurcumin-Formulated Chitosan-g-Pluronic Hydrogel Exhibiting a Great Potential for Burn Treatment

Journal of Healthcare Engineering ◽

10.1155/2018/5754890 ◽

2018 ◽

Vol 2018 ◽

pp. 1-14 ◽

Cited By ~ 18

Author(s):

Le Hang Dang ◽

Thi Hiep Nguyen ◽

Ha Le Bao Tran ◽

Vu Nguyen Doan ◽

Ngoc Quyen Tran

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Healing Process ◽

Nanocomposite Hydrogel ◽

Burn Wound ◽

Burn Treatment ◽

Nanocomposite Hydrogels ◽

Burn Wound Healing

Burn wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Curcumin is believed to be a potent antioxidant and anti-inflammatory agent; therefore, it can prevent the prolonged presence of oxygen free radicals which is a significant factor causing inhabitation of optimum healing process. This study describes an extension of study about the biofunctional nanocomposite hydrogel platform that was prepared by using curcumin and an amphiphilic chitosan-g-pluronic copolymer specialized in burn wound healing application. This formular (nCur-CP, nanocomposite hydrogel) was a free-flowing sol at ambient temperature and instantly converted into a nonflowing gel at body temperature. In addition, the storage study determined the great stability level of nCur-CP in long time using UV-Vis and DLS. Morphology and distribution of nCur in its nanocomposite hydrogels were observed by SEM and TEM, respectively. In vitro studies suggested that nCur-CP exhibited well fibroblast proliferation and ability in antimicrobacteria. Furthermore, second- and third-degree burn wound models were employed to evaluate the in vivo wound healing activity of the nCur-CP. In the second-degree wound model, the nanocomposite hydrogel group showed a higher regenerated collagen density and thicker epidermis layer formation. In third degree, the nCur-CP group also exhibited enhancement of wound closure. Besides, in both models, the nanocomposite material-treated groups showed higher collagen content, better granulation, and higher wound maturity. Histopathologic examination also implied that the nanocomposite hydrogel based on nanocurcumin and chitosan could enhance burn wound repair. In conclusion, the biocompatible and injectable nanocomposite scaffold might have great potential to apply for wound healing.

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3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing

Regenerative Biomaterials ◽

10.1093/rb/rbab014 ◽

2021 ◽

Vol 8 (3) ◽

Author(s):

Yu Wu ◽

Tangzhao Liang ◽

Ying Hu ◽

Shihai Jiang ◽

Yuansen Luo ◽

...

Keyword(s):

Wound Healing ◽

Umbilical Vein ◽

3D Bioprinting ◽

Severe Burn ◽

Burn Wound ◽

Hydrogel Scaffold ◽

Hydrogel Scaffolds ◽

Burn Wound Healing ◽

Severe Burns

Abstract Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but are impeded by their low viability and efficiency after grafting in vivo. Nitric oxide (NO) is beneficial in promoting stem cell bioactivity, but whether it can function effectively in vivo is still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs and NO (3D-ADSCs/NO) to evaluate its biological efficacy in promoting severe burn wound healing. The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting. Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs). Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition. Notably, immunohistochemistry of CD31 suggested an increase in neovascularization, supported by the upregulation of vascular endothelial growth factor (VEGF) mRNA in ADSCs in the 3D biosystem. These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway. This scaffold may be considered a promising strategy for healing severe burns.

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Laryngeal Thermal Injury Model

Journal of Burn Care & Research ◽

10.1093/jbcr/iraa009 ◽

2020 ◽

Vol 41 (3) ◽

pp. 626-632

Author(s):

Gregory R Dion ◽

Christian S Pingree ◽

Pedro J Rico ◽

Christine L Christensen

Keyword(s):

Wound Healing ◽

Thermal Injury ◽

Injury Severity ◽

Vocal Folds ◽

Burn Wound ◽

Burn Wound Healing ◽

Microscopic Evaluation ◽

Thermal Injuries ◽

False Vocal ◽

Over Time

Abstract A lack of reliable laryngeal thermal injury models precludes laryngeal burn wound healing studies and investigation of novel therapeutics. We hypothesize that a swine laryngeal burn model can allow for laryngeal burn evaluation over time. Twelve Yorkshire crossbreed swine underwent tracheostomy and endoscopically directed laryngeal burns using heated air (150–160°C). Swine larynges were evaluated and sectioned/stained at 12 hours, 1, 3, 7, 14, and 21 days. A board-certified veterinary pathologist assessed anatomic regions (left and right: epiglottis, true/false vocal folds, and subglottis) using a nine criteria histological injury scoring scale. Six swine were euthanized at scheduled endpoints, three prematurely (airway concerns), and three succumbed to airway complications after 16 to 36 hours. Endoscopic and gross examination from scheduled endpoints revealed massive supraglottic edema and tissue damage, particularly around the arytenoids, extending transglottically. Swine from premature endpoints had comparatively increased edema throughout. Microscopic evaluation documented an inverse relationship between injury severity score and time from injury. Inflammation severity decreased over time, nearly resolving by 14 days. Neutrophils predominated early with histiocytes appearing at 3 days. Granulation tissue appeared at 3 days, and early epiglottic and/or subglottic fibrosis appeared by 7 days and matured by 14 days. Edema, abundant initially, decreased by day 3 and resolved by day 7. This approach is the first to provide longitudinal analysis of laryngeal thermal injuries, reflecting some of the first temporal wound healing characteristic data in laryngeal thermal injuries and providing a platform for future therapeutic studies.

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