scholarly journals Gadolinium Retention and Clearance in the Diabetic Brain after Administrations of Gadodiamide, Gadopentetate Dimeglumine, and Gadoterate Meglumine in a Rat Model

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Shu-ting Wang ◽  
Zheng-xu Hua ◽  
Dong-xiao Fan ◽  
Xin Zhang ◽  
Ke Ren
Keyword(s):  
Inductively Coupled Plasma ◽  
Gadopentetate Dimeglumine ◽  
Cerebellar Nuclei ◽  
Diabetic Rats ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Gadoterate Meglumine ◽  
Significant Difference ◽  
Magnetic Resonance Imaging Mri ◽  
The Brain

Purpose. To evaluate gadolinium (Gd) retention and clearance in the brain of diabetic rats after administrations of gadodiamide, gadopentetate dimeglumine, and gadoterate meglumine. Materials and Methods. Both diabetic rats (n = 52) and normal rats (n = 52) intravenously received 20 injections of 0.6 mmol Gd/kg gadodiamide, gadopentetate dimeglumine, gadoterate meglumine, or saline. Both diabetic rats and normal rats were divided into 2 subgroups of 24 and 28 rats for the 7-day and 42-day evaluations (i.e., they were sacrificed at 7 days (n = 6 per group) and 42 days (n = 7 per group)), respectively, after the last injection. For the 7-day subgroup, 6 rats were euthanized for inductively coupled plasma mass spectrometry (ICP-MS) analysis. For the 42-day subgroup, 6 rats underwent T1-weighted magnetic resonance imaging (MRI) and ICP-MS, and 1 rat was analyzed by transmission electron microscopy (TEM). Results. The T1 enhancements in the deep cerebellar nuclei (DCNs) of diabetic rats were lower than those of normal rats in both linear Gd-based contrast agent (GBCA) groups (p < 0.05). The average Gd concentrations in the brains of diabetic rats were significantly lower than those of healthy rats in both the short-term groups and long-term groups (p < 0.05). The highest Gd retentions were in the olfactory bulb, DCN, and striatum with gadodiamide. Compared with the results obtained 7 days after the last injection, the residual Gd concentrations of the 42-day subgroups in the brains of diabetic rats showed no significant difference in both linear GBCA groups (p>0.05). Conclusions. Compared with normal rats, the diabetic status decreased the residual Gd concentrations in the brain after multiple administrations of gadodiamide, gadopentetate dimeglumine, and gadoterate meglumine. The clearable fraction of Gd in the brain was eliminated faster in diabetic rats than in normal rats.

scholarly journals Arsenic Accumulation of Realgar Altered by Disruption of Gut Microbiota in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Wenfeng Xu ◽  
Shanshan Zhang ◽  
Wenqing Jiang ◽  
Shuo Xu ◽  
Pengfei Jin
Keyword(s):  
Gut Microbiota ◽  
Whole Blood ◽  
Subcutaneous Injection ◽  
Inductively Coupled Plasma ◽  
High Dose ◽  
Inductively Coupled ◽  
Arsenic Accumulation ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

Objective. To investigate the influence of gut microbiota on arsenic accumulation of realgar in mice. Methods. Mice were treated with antibiotics to form a mouse model of gut microbial disruption. Antibiotic-treated and normally raised mice were given 15 mg/kg, 150 mg/kg, and 750 mg/kg realgar by gavage and 0.2 mg/kg and 1 mg/kg arsenic solution by subcutaneous injection for 7 days. The concentration of arsenic in mice whole blood was determined by inductively coupled plasma mass spectrometry (ICP-MS). Arsenic accumulation in antibiotic-treated mice and normally raised mice was compared. Results. After exposure to low dose (15 mg/kg) and middle dose (150 mg/kg) of realgar, significantly, more arsenic was accumulated in the whole blood of antibiotic-treated mice compared to normally raised counterparts, which indicated that the disruption of gut microbiota could lead to higher arsenic load of realgar in mice. The homeostasis of gut microbiota was supposed to be disrupted by high dose (750 mg/kg) of realgar because after exposure to high dose of realgar, there was no significant difference in arsenic accumulation between antibiotic-treated and normally raised mice. Furthermore, arsenic solution was administered by subcutaneous injection to mice to investigate the influence of gut microbial differences on arsenic accumulation in addition to the absorption process, and there was no significant difference in arsenic accumulation between mice with these two different statuses of gut microbiota. Conclusions. Gut microbiota disruption could increase arsenic accumulation of realgar in mice.

scholarly journals Hair Mercury Levels Detection in Fishermen from Sicily (Italy) by ICP-MS Method after Microwave-Assisted Digestion

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Giuseppe Giangrosso ◽  
Gaetano Cammilleri ◽  
Andrea Macaluso ◽  
Antonio Vella ◽  
Nicolantonio D’Orazio ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Total Mercury ◽  
Control Group ◽  
Inductively Coupled ◽  
Hair Mercury ◽  
Icp Ms ◽  
Hair Samples ◽  
Significant Difference ◽  
Close Relationship ◽  
Plasma Mass Spectrometry

A number of ninety-six hair samples from Sicilian fishermen were examined for total mercury detection by an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) method. The mercury levels obtained were compared with mercury levels of 96 hair samples from a control group, in order to assess potential exposure to heavy metals of Sicilian fishermen due to fish consumption and closeness to industrial activities. Furthermore, the mercury levels obtained from hair samples were sorted by sampling area in order to verify the possible risks linked to the different locations. The overall mean concentration in the hair of the population of fishermen was6.45±7.03 μg g−1, with a highest value in a fisherman of Sciacca (16.48 μg g−1). Hair mercury concentration in fishermen group was significantly higher than in control group (p<0.01). There was no significant difference in hair total mercury concentrations between sampling areas (p>0.05). The results of this study indicate a greater risk of exposure to mercury in Sicilian fishermen, in comparison to the control population, due to the high consumption of fish and the close relationship with sources of exposure (ports, dumps, etc.).

scholarly journals Analysis of Sr and Ba profiles measured by ultra-high-resolution mass spectrometry LA-ICP-MS in otoliths of juvenile anadromous sockeye salmon Oncorhynchus nerka in the early marine life-history stage as a proxy for fresh to marine water transition

2021 ◽  
Vol 201 (3) ◽  
pp. 669-685
Author(s):  
Yu. Egorova ◽  
Yu. Kuzmenko ◽  
T. Spesivy ◽  
B. P.V. Hunt ◽  
E. A. Pakhomov
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Sockeye Salmon ◽  
High Resolution Mass Spectrometry ◽  
Oncorhynchus Nerka ◽  
Life History Stage ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Reliable Marker

The strontium (Sr) and barium (Ba) profiles in otoliths of juvenile sockeye salmon Oncorhynchus nerka from British Columbia are measured using a Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) system and analyzed. The highest possible measurement resolution (near-daily) was used to assess variability and repeatability of the breakpoint (marine entry) estimates inferred from Sr:Ca and Ba:Ca ratios. Such resolution for the otolith chemical composition (to an accuracy of 2 μm) was reached using the rotating slit, which width was close to the daily circulus width of the otoliths. So, daily or 2-day changes in the elemental composition were recorded during the period of transition to the marine environment. Sr profiles were generally similar among the fish, starting with low values of Sr:Ca in the fresh water and increasing sharply after the marine entry. The Ba:Ca signal was more complex, showing in most cases a dramatic increase immediately before the breakpoint. Besides, multiple peaks in the Ba profiles were recorded prior to the marine transition with a significant difference of their number between fish from different populations. A breakpoint was detectable in the Ba profiles 3–11 µm prior to its appearance in the Sr profiles. The complexity of Ba profiles may cause erroneous estimates of the marine entry date; thus, the Sr signal is a more reliable marker of marine transition for juvenile sockeye. 

scholarly journals Obtaining information from the brain in a non-invasive way: determination of iron in nasal exudate to differentiate hemorrhagic and ischemic strokes

2020 ◽  
Vol 58 (5) ◽  
pp. 847-853 ◽  
Author(s):  
Carmen García-Cabo ◽  
Pablo Llano-Suárez ◽  
Lorena Benavente-Fernández ◽  
Sergio Calleja-Puerta ◽  
José Manuel Costa-Fernández ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Characteristic Curve ◽  
Iron Concentration ◽  
Sampling Procedure ◽  
Non Invasive ◽  
Icp Ms ◽  
Significant Difference ◽  
Promising Source ◽  
The Brain

AbstractBackgroundDifferentiation between hemorrhagic and ischemic stroke is currently made by brain imaging or analyzing blood and cerebrospinal fluid (CSF) samples. After describing a new drainage route from brain to nasal mucosa, nasal exudate samples can be considered a new and promising source of biomarkers. Saliva can also be evaluated.MethodsWe determined iron in nasal exudate and saliva samples from patients of acute stroke during the first 48 h from onset. A simple, non-invasive sampling procedure was employed to obtain information from the brain. Samples were taken with a pre-weighed swab, solved in a 2% nitric acid solution and iron was measured by inductively coupled plasma-tandem mass spectrometry (ICP-MS/MS).ResultsA significant difference in the dispersion of results of iron concentration for both stroke subtypes was observed in nasal exudate samples. The interquartile range was 0.608 nmol mg−1 of iron for hemorrhagic strokes and only 0.044 nmol mg−1 for ischemic strokes. In saliva samples, however, the values were 0.236 vs. 0.157 nmol mg−1. A cut-off limit of 0.102 nmol of iron per mg of nasal exudate provides a methodology with a 90% of sensitivity and a 90% of specificity. The value of the area under (AUC) the receiver operating characteristic curve (ROC) for nasal exudate samples is 0.960, considered as very good in which regards to its predictive value.ConclusionsNon-invasive samples of nasal secretion have allowed obtaining, for the first time, information from the brain. Determination of iron in nasal exudate by ICP-MS allowed differentiation between ischemic and hemorrhagic strokes.

scholarly journals Evaluation of an Element-Tagged Duplex Immunoassay Coupled with Inductively Coupled Plasma Mass Spectrometry Detection: A Further Study for the Application of the New Assay in Clinical Laboratory

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5370 ◽  
Author(s):  
Wencan Jiang ◽  
Gongwei Sun ◽  
Wenbin Cui ◽  
Shasha Men ◽  
Miao Jing ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Clinical Laboratory ◽  
Clinical Sample ◽  
Measurement Range ◽  
Clinical Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry

Background: Element-tagged immunoassay coupled with inductively coupled plasma mass spectrometry (ICP-MS) detection has the potential to revolutionize immunoassay analysis for multiplex detection. However, a further study referring to the standard evaluation and clinical sample verification is needed to ensure its reliability for simultaneous analysis in clinical laboratories. Methods: Carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) were chosen for the duplex immunoassay. The performance of the assay was evaluated according to guidelines from the Clinical and Laboratory Standards Institute (CLSI). Moreover, reference intervals (RIs) of CEA and AFP were established. At last, 329 clinical samples were analyzed by the proposed method and results were compared with those obtained with electrochemiluminescent immunoassay (ECLIA) method. Results: The measurement range of the assay was 2–940 ng/mL for CEA and 1.5–1000 ng/mL for AFP, with a detection limit of 0.94 ng/mL and 0.34 ng/mL, respectively. The inter-assay and intra-assay imprecision were all less than 6.58% and 10.62%, respectively. The RI of CEA and AFP was 0–3.84 ng/mL and 0–9.94 ng/mL, respectively. Regarding to clinical sample detection, no significant difference was observed between the proposed duplex assay and the ECLIA method. Conclusions: The ICP-MS-based duplex immunoassay was successfully developed and the analytical performance fully proved clinical applicability. Well, this could be different with other analytes.

scholarly journals Health Risks Assessment of Rainwater Pollutants in Jengka, Pahang, Malaysia

2018 ◽  
Vol 7 (3.30) ◽  
pp. 15
Author(s):  
Siti Nadzifah Ghazali ◽  
Fazrul Razman ◽  
Mohd Zahari Abdullah
Keyword(s):  
Heavy Metals ◽  
Inductively Coupled Plasma ◽  
Principal Component ◽  
Anthropogenic Sources ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
Health Risks Assessment ◽  
Risks Assessment

Rainwater samples were collected in Jengka, Pahang Malaysia. Temperature, pH, TDS, EC, Al, Cu, Mn, and Zn were measured. The concentrations of heavy metals (Al, Cu, Mn and Zn) in thirty-eight samples were analysed using inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis results indicate that pH, TDS, and EC showed significant differences between wet and dry seasons. However, no significant difference was observed seasonally for heavy metals (HMs). All parameters, except pH, were below the Malaysian Drinking Water Guidelines (MDWG) value and the health risk assessment for HMs indicates the safe levels. Principal component analysis (PCA) suggests that HMs in rainwater in Jengka, Pahang were originated from natural and anthropogenic sources.  

scholarly journals Rare earth elements in oyster shells: provenance discrimination and potential vital effects

2020 ◽  
Vol 17 (8) ◽  
pp. 2205-2217
Author(s):  
Vincent Mouchi ◽  
Camille Godbillot ◽  
Vianney Forest ◽  
Alexey Ulianov ◽  
Franck Lartaud ◽  
...  
Keyword(s):  
Rare Earth ◽  
Rare Earth Elements ◽  
Inductively Coupled Plasma ◽  
Hydrothermal Activity ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
Vital Effects ◽  
Bivalve Shells

Abstract. Rare earth elements (REEs) and yttrium in seawater originate from atmospheric fallout, continental weathering, and transport from rivers, as well as hydrothermal activity. Previous studies have reported the use of REE and Y measurements in biogenic carbonates as a means to reconstruct these surface processes in ancient times. As coastal seawater REE and Y concentrations partially reflect those of nearby rivers, it may be possible to obtain a regional fingerprint of these concentrations from bivalve shells for seafood traceability and environmental monitoring studies. Here, we present a dataset of 297 measurements of REE and Y abundances by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) from two species (Crassostrea gigas and Ostrea edulis). We measured a total of 49 oyster specimens from six locations in France (Atlantic Ocean and Mediterranean Sea). Our study reports that there is no significant difference in concentrations from shell parts corresponding to winter and summer periods for both species. Moreover, interspecific vital effects are reported from specimens from both species and from the same locality. REE and Y profiles as well as t-distributed stochastic neighbour embedding processing (t-SNE; a discriminant statistical method) indicate that REE and Y measurements from C. gigas shells can be discriminated from one locality to another, but this is not the case for O. edulis, which presents very similar concentrations in all studied localities. Therefore, provenance studies using bivalve shells based on REEs and Y have to first be tested for the species. Other methods have to be investigated to be able to find the provenance of some species, such as O. edulis.

scholarly journals The Distribution of the Heavy Metals in Soil Sample Along Blue Nile During overflow Season

2017 ◽  
Vol 10 (1) ◽  
pp. 19
Author(s):  
Magdi H. Saad
Keyword(s):  
Heavy Metals ◽  
Inductively Coupled Plasma ◽  
Blue Nile ◽  
Heavy Metal Concentrations ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Zn Concentration ◽  
Significant Difference ◽  
Plasma Mass Spectrometry ◽  
Two Sides

The research cared out to calculate the heavy metals deposition for the soil of the Blue Nile along the edges of two sides, starting from the Sudan Ethiopia border end to the Khartoum state in Tuti Island. The samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) technique. The results show that there is a significant difference for most of the concentration of the elements deposition on two sides of the Blue Nile outside of Nile channel for that area which covered by water after the overflow. Heavy metals Cr, Fe, Co, Cu, and Zn concentration deposition are increased in the direction of the water to Khartoum, while Ni and Pb concentrations are decreased in the same direction. K, Ca, Ti, Mn, Br, Rb, Sr, and Zr heavy metal concentrations are varied but in general remains equals.

Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
Bhagavathami Meenaksh ◽  
Chitraa R. Chandran ◽  
Aravindhan Thathchari Ranganathan ◽  
Kavindapadi Venkateswaran Rama ◽  
Valarmathy Srinivasan
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Gingival Crevicular Fluid ◽  
Serum Zinc ◽  
Cross Sectional Study ◽  
Serum Copper ◽  
Cross Sectional ◽  
Inductively Coupled ◽  
Significant Difference ◽  
Copper Zinc

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

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