scholarly journals A New Quinone Based Fluorescent Probe for High Sensitive and Selective Detection of Biothiols and Its Application in Living Cell Imaging

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Yanju Liu ◽  
Manman Li ◽  
Keith Man-Chung Wong ◽  
Yan Tong ◽  
Huaixia Yang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Density Functional ◽  
Vital Role ◽  
Confocal Imaging ◽  
Selective Detection ◽  
Physiological Concentration ◽  
Physiological Processes ◽  
Fluorescence Confocal Imaging ◽  
Living Cell Imaging

In view of the vital role of biothiols in many physiological processes, the development of simple and efficient probe for the detection of biothiols is of great medical significance. In this work, we demonstrate the use of 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ), which respond rapidly to biothiols especially to glutathione, as a new fluorescent probe for the selective detection and bioimaging of biothiols. This new fluorescent probe can distinguish glutathione from cysteine and homocysteine easily under physiological concentration and detect glutathione quickly within three minutes. This probe exhibits high selectivity to biothiols and the detection limit was determined to be 3.08 × 10−9 M for glutathione, 8.55 × 10−8 M for cysteine, and 2.17 × 10−9 M for homocysteine, respectively. The sensing mechanism was further explored by density functional theory (DFT) and nuclear magnetic resonance (NMR) experiment; results showed that the interaction forces between the probe and biothiols were electrostatic interaction. In addition, the probe has been successfully applied to the detection of biothiols in Eca9706 cells by fluorescence confocal imaging technology.

scholarly journals The role of TGF-β2 in cartilage development and diseases

2021 ◽  
Vol 10 (8) ◽  
pp. 474-487
Author(s):  
Mengmeng Duan ◽  
Qingxuan Wang ◽  
Yang Liu ◽  
Jing Xie
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Vital Role ◽  
Cartilage Defects ◽  
Cartilage Development ◽  
Physiological Processes ◽  
Cartilage Diseases ◽  
Autologous Mesenchymal Stem Cells ◽  
Tgf Β1

Transforming growth factor-beta2 (TGF-β2) is recognized as a versatile cytokine that plays a vital role in regulation of joint development, homeostasis, and diseases, but its role as a biological mechanism is understood far less than that of its counterpart, TGF-β1. Cartilage as a load-resisting structure in vertebrates however displays a fragile performance when any tissue disturbance occurs, due to its lack of blood vessels, nerves, and lymphatics. Recent reports have indicated that TGF-β2 is involved in the physiological processes of chondrocytes such as proliferation, differentiation, migration, and apoptosis, and the pathological progress of cartilage such as osteoarthritis (OA) and rheumatoid arthritis (RA). TGF-β2 also shows its potent capacity in the repair of cartilage defects by recruiting autologous mesenchymal stem cells and promoting secretion of other growth factor clusters. In addition, some pioneering studies have already considered it as a potential target in the treatment of OA and RA. This article aims to summarize the current progress of TGF-β2 in cartilage development and diseases, which might provide new cues for remodelling of cartilage defect and intervention of cartilage diseases.

scholarly journals The critical role of Krüppel-like factors in kidney disease

2017 ◽  
Vol 312 (2) ◽  
pp. F259-F265 ◽  
Author(s):  
Sandeep K. Mallipattu ◽  
Chelsea C. Estrada ◽  
John C. He
Keyword(s):  
Current Knowledge ◽  
Critical Role ◽  
Vital Role ◽  
Glomerular Filtration Barrier ◽  
Kidney Fibrosis ◽  
Physiological Processes ◽  
Filtration Barrier ◽  
And Function ◽  
Mammalian Embryonic Development

Krüppel-like factors (KLFs) are a family of zinc-finger transcription factors critical to mammalian embryonic development, regeneration, and human disease. There is emerging evidence that KLFs play a vital role in key physiological processes in the kidney, ranging from maintenance of glomerular filtration barrier to tubulointerstitial inflammation to progression of kidney fibrosis. Seventeen members of the KLF family have been identified, and several have been well characterized in the kidney. Although they may share some overlap in their downstream targets, their structure and function remain distinct. This review highlights our current knowledge of KLFs in the kidney, which includes their pattern of expression and their function in regulating key biological processes. We will also critically examine the currently available literature on KLFs in the kidney and offer some key areas in need of further investigation.

scholarly journals Exosomes: A Novel Therapeutic Paradigm for Treatment of Depression

2020 ◽  
Vol 21 ◽  
Author(s):  
Shvetank Bhatt ◽  
Jovita Kanoujia ◽  
Arghya Kusum Dhar ◽  
Surendar Arumugam ◽  
Amanda K. A. Silva ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Small Rna ◽  
Potential Role ◽  
Vital Role ◽  
Physiological Processes ◽  
Treatment Of Depression ◽  
As Stress ◽  
Novel Therapeutic

Abstract: Extracellular vesicles (EVs) of endocytic origin are known as exosomes. These vesicles are released by cells and are accessible in biofluids, such as saliva, urine, and plasma. These vesicles are made up of small RNA, DNA, proteins and play a vital role in many physiological processes. In central nervous system (CNS), they participate in various physiological processes such as stress of nerve cells, communication between the cells, synaptic plasticity and neurogenesis. The role of exosomes in depression needs to be explored further. It is known that exosomes can cross blood brain barrier (BBB), which is made up of glial cells astrocytes. One of the advantages of these vescicles is that they are able to transfer macromolecules like DNA, protein, mRNAs and miRNAs to recipient cells. This review focuses on the potential role of exosomes in de-pression and their utilization as atreatmentoption or diagnostic tool of depression.

A new fluorescent probe for quick and highly selective detection of hydrogen sulfide and its application in living cells

2018 ◽  
Vol 42 (16) ◽  
pp. 13884-13888 ◽  
Author(s):  
Yanshen Ren ◽  
Lulu Zhang ◽  
Ziyan Zhou ◽  
Suilou Wang ◽  
Yungen Xu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Selective Detection ◽  
Physiological Processes ◽  
Endogenous Regulators

Hydrogen sulfide (H2S) is one of the endogenous regulators of many physiological processes.

scholarly journals Mitophagy in carcinogenesis, drug resistance and anticancer therapeutics

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yanjie Guan ◽  
Yifei Wang ◽  
Bo Li ◽  
Kai Shen ◽  
Quanfu Li ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Energy Production ◽  
Cellular Differentiation ◽  
Vital Role ◽  
Regulatory Mechanisms ◽  
Physiological Processes ◽  
Promising Strategy ◽  
New Frontier ◽  
Anticancer Therapeutics

AbstractThe mitochondrion is an organelle that plays a vital role in energy production, cytoplasmic protein degradation and cell death. Mitophagy is an autophagic procedure that specifically clears damaged mitochondria and maintains its homeostasis. Emerging evidence indicates that mitophagy is involved in many physiological processes, including cellular homeostasis, cellular differentiation and nerve protection. In this review, we describe the regulatory mechanisms of mitophagy in mammals and yeasts and highlight the recent advances relevant to its function in carcinogenesis and drug resistance. Finally, a section has been dedicated to describing the role of mitophagy in anticancer therapeutics, which is a new frontier that offers a precise and promising strategy.

scholarly journals A 1,8-naphthalimide-based fluorescent probe for selective and sensitive detection of peroxynitrite and its applications in living cell imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (54) ◽  
pp. 34287-34292 ◽  
Author(s):  
Xiulan Li ◽  
Jingli Hou ◽  
Chao Peng ◽  
Li Chen ◽  
Wenbo Liu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cell ◽  
Cell Imaging ◽  
Sensitive Detection ◽  
Selective Detection ◽  
Low Cytotoxicity ◽  
Living Cell Imaging

An “off–on” fluorescent probe for sensitive and selective detection of peroxynitrite was synthesized and showed good photostability and low cytotoxicity.

Tomato Protein Phosphatase 2C (SlPP2C3) influences fruit ripening onset and fruit glossiness

2020 ◽  
Author(s):  
Bin Liang ◽  
Yufei Sun ◽  
Juan Wang ◽  
Yu Zheng ◽  
Wenbo Zhang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Vital Role ◽  
Negative Regulator ◽  
Rna Seq ◽  
Tomato Plants ◽  
Enhanced Sensitivity ◽  
Physiological Processes ◽  
Significant Difference ◽  
Epidermis Structure

Abstract Abscisic acid (ABA) plays a vital role in coordinating physiological processes during fresh fruit ripening. Binding of ABA to receptors facilitates the interaction and inhibition of type 2C phosphatase (PP2C) co-receptors. However, the exact mechanism of PP2C during fruit ripening is unclear. In this study, we determined the role of tomato ABA co-receptor type 2C phosphatase SlPP2C3, a negative regulator of ABA signalling and fruit ripening. SlPP2C3 selectively interacted with monomeric ABA receptors and SlSnRK2.8 kinase in both yeast and tobacco epidermal cells. Expression of SlPP2C3 was ABA-inducible, which was negatively correlated with fruit ripening. Tomato plants with suppressed SlPP2C3 expression exhibited enhanced sensitivity to ABA, while plants over-expressing SlPP2C3 were less sensitive to ABA. Importantly, lack of SlPP2C3 expression accelerated fruit ripening onset and affected fruit glossiness by altering the outer epidermis structure. There was a significant difference in expression of cuticle-related genes in pericarp between wild-type and SlPP2C3 suppressed lines based on RNA sequencing (RNA-seq) analysis. Taken together, our findings demonstrate that SlPP2C3 plays an important role in the regulation of fruit ripening and fruit glossiness in tomato.

scholarly journals Tomato Protein Phosphatase 2C (SlPP2C3) negatively regulates fruit ripening onset and fruit gloss

2020 ◽  
Author(s):  
Bin Liang ◽  
Yufei Sun ◽  
Juan Wang ◽  
Yu Zheng ◽  
Wenbo Zhang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Vital Role ◽  
Negative Regulator ◽  
Aba Signaling ◽  
Rna Seq ◽  
Enhanced Sensitivity ◽  
Physiological Processes ◽  
Appearance Quality ◽  
Aba Receptors

AbstractAbscisic acid (ABA) plays a vital role in coordinating physiological processes during fresh fruit ripening. ABA can bind to ABA receptors which interacts and inhibits their co-receptors type 2C phosphatases (PP2Cs). However, the dissected mechanism of PP2C during fruit ripening is unclear. In this study, we identify the role of SlPP2C3, a tomato type 2C phosphatase, as a negative regulator of ABA signaling and fruit ripening. SlPP2C3 selectively interacted with monomeric ABA receptors and SlSnRK2.8 kinase in both yeast and tobacco epidermal cells. Expressions of SlPP2C3 were observed in all tissues, and it negatively correlated with the fruit ripening which was induced by exogenous ABA. Tomato plants with suppressed SlPP2C3 expression exhibited enhanced sensitivity to ABA, while SlPP2C3 over-expressed plants were less sensitive to ABA. Meaningfully, lack of SlPP2C3 expression causes the acceleration of fruit ripening onset via the alternation of ABA signaling activity, and the fruit gloss is affected by the changes of outer epidermis structure. RNA-seq analysis found significant different expression of cuticle-related genes in pericarp between wild-type and SlPP2C3 suppressed lines. Taken together, our finding demonstrate that SlPP2C3 plays an important role in the regulation of fruit ripening and fruit appearance quality in tomato.

An ESIPT-based colorimetric and fluorescent probe with large Stokes shift for the sensitive detection of hypochlorous acid and its bioimaging in cells

2021 ◽  
Vol 45 (10) ◽  
pp. 4724-4728
Author(s):  
Haixian Ren ◽  
Fangjun Huo ◽  
Caixia Yin
Keyword(s):  
Immune System ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Stokes Shift ◽  
Vital Role ◽  
Human Immune System ◽  
Physiological Concentration ◽  
System P ◽  
Human Immune ◽  
Anti Inflammation

Hypochlorous acid (HOCl), with a low physiological concentration, plays a vital role in killing the pathogens and anti-inflammation in the human immune system.

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