scholarly journals Parathormone Levels in a Middle-Eastern Healthy Population Using 2nd and 3rd Generation PTH Assays

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Marie-Hélène Gannagé-Yared ◽  
Marie-Noëlle Kallas-Chémaly ◽  
Ghassan Sleilaty
Keyword(s):  
Vitamin D ◽  
Healthy Subjects ◽  
Reference Range ◽  
Middle Eastern ◽  
Healthy Population ◽  
Strongly Correlated ◽  
Limits Of Agreement ◽  
25 Oh Vitamin D ◽  
D Values ◽  
Normal Calcium

Background. The purpose of the current study is to determine PTH reference values in vitamin-D-replete Lebanese adults using 2nd and 3rd generation PTH assays and to look at the factors that affect PTH variations. Methods. Fasting PTH was measured using 2nd and 3rd generation Diasorin PTH assays in 339 vitamin-D-replete healthy subjects aged 18 to 63 years (230 men and 109 women) who have normal calcium levels and an eGFR ≥60 ml/mn. 25-OH vitamin D (25(OH)D) was measured using the Diasorin assay. Results. For the 2nd PTH generation, median (IQR) levels were 48.9 (34.9–66.0) pg/ml, and its 2.5th–97.5th percentile values were 19.7–110.5 pg/ml for 25(OH)D values between 20 and 30 ng/ml, and 19.7–110.7 pg/ml for 25(OH)D values ≥30 ng/ml. For the 3rd PTH generation, the median (IQR) values were 23.9 (17.7–30.5) pg/ml, and its 2.5th–97.5th percentile values were, respectively, 9.2 and 50.2 pg/ml for 25(OH)D values between 20 and 30 ng/ml, and 8.4 and 45.4 pg/ml for 25(OH)D values ≥30 ng/ml. The median (IQR) serum 25(OH)D levels were 27.5 (23.8–32.7) ng/ml. 2nd and 3rd generation PTH values are strongly correlated (r = 0.96, p<0.0001), but poorly concordant (Lin’s concordance coefficient 0.365, 95% CI: 0.328–0.401) with observations beyond the 95% Bland–Altman limits of agreement. 2nd and 3rd generation PTH levels did not differ according to gender and were significantly correlated with age but not with 25(OH)D and serum calcium levels. Conclusion. Lebanese adult healthy subjects have higher 2nd and 3rd generation PTH levels compared with the reference range provided by the manufacturer. The reference range was not influenced by changing the 25(OH)D cutoff. The clinical significance of the higher PTH levels in our population should be investigated.

scholarly journals OSTEOPENIA IN CHILDREN WITH MALABSORPTION SYNDROM

2016 ◽  
Vol 65 (4) ◽  
pp. 368-372
Author(s):  
Tania Elena Rusu ◽  
◽  
Evelina Moraru ◽  
Laura Bozomitu ◽  
Dana Teodora Anton Paduraru ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Vitamin D ◽  
Celiac Disease ◽  
Bone Density ◽  
Inflammatory Marker ◽  
Z Score ◽  
Malabsorption Syndromes ◽  
25 Oh Vitamin D ◽  
D Values ◽  
And Function

Introduction. Malabsorption syndromes result in the disturbance of bone normal development and function. Objective. The assessment of bone density in children with malabsorption syndromes. The analysis of risk factors for osteopenia. The correlation between osteopenia, nutritional markers and bone metabolism markers. Material and method. 118 children with malabsorbtion syndromes of different etiologies, mainly Celiac disease (41 cases) and Cystic fibrosis (14 cases). Bone density was assessed by Quantitative Ultrasonography (QUS) with a Sunlight Omnisense Ultrasonometer 7000P. QUS was performed at two sites – radius (86 cases) and tibia (78 cases). 25hydroxivitamin D was measured in 10 cases by RIA method. Statistical analysis was made using SPSS for Windows. Results. Osteopenia was present in 32% cases. Decreased bone density at the radius was associated with the celiac syndrome. Osteopenia at the tibia was associated with cystic fibrosis. Osteopenia was more frequent in girls. Osteopenia was related to the duration of the disease. Osteopenia wasn’t related to BMI. Radius Z-score positively correlated to alkaline phosphatase levels and tibia Z-score to serum cholesterol levels. Radius and tibia Z-score negatively correlated with inflammatory marker levels. In children with celiac disease, the value of anti-transglutaminase antibodies was negatively correlated to radius and tibia Z-score values. 25(OH) vitamin D values were deficient in 8 patient and insufficient in 2, but its values didn’t correlate to radius/tibia Z-score. Clinical, biological and radiological signs of rickets were found in 35% of patients with osteopenia. Conclusions. Osteopenia was found in 1/3 of patients with malabsorption syndromes of the studied group. In 30% of patients, clinical, biological and radiologic rickets signs were present. 25(OH) vitamin D values didn’t correlate with the Z-score. A negative correlation between bone parameters and inflammation markers and anti-transglutaminase antibodies values was observed.

Vitamin D Deficiency, Prevalence and Treatment in Neonatal Period

2019 ◽  
Vol 19 (6) ◽  
pp. 866-873 ◽  
Author(s):  
Atiye Fedakâr
Keyword(s):  
Intensive Care Unit ◽  
Vitamin D ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Vitamin D Deficiency ◽  
Neonatal Intensive Care ◽  
Maternal Vitamin ◽  
Positive Correlation ◽  
25 Oh Vitamin D ◽  
D Values

Objective: Maternal vitamin D deficiency is an important risk factor that causes infantile rickets in the neonatal and infantile period. The aim of this study was to review the prevalence, clinical characteristics, and treatment of vitamin D deficiency and the follow-ups with infants and their mothers by the neonatal intensive care unit of Afiyet Hospital in Turkey. Methods: Calcium (Ca), phosphorus (P) and 25 (OH) vitamin D were studied and prospectively recorded in infants and their mothers detected to have hypocalcemia during routine biochemistry tests performed on the third postnatal day of the patients follow up and treated with different diagnoses. Results: A total of 2,460 infants were admitted into the neonatal intensive care unit between August 2014 and January 2018. Of the infants included in the study, 324 (66.1%) were male and 166 (33.8%) were female, and 366 (74.6%) of them had been delivered by cesarean section (C/S), 124 (25.3%) of them had been delivered by Normal Spontaneous Delivery (NSD). Hypocalcemia was detected in 490 (19.9%) of the infants. In a total of 190 (38.7%) infants and 86 mothers (17.5%), the levels of 25 (OH) vitamin D were found to be below the laboratory detection limit of <3 ng/ml. When vitamin D deficiency + insufficiency is assessed by season, 151 of them were found to be in summer (30.99%), 118 in spring (24.18%), 117 in the winter season(23.87%), and 93 in autumn(18.97%), respectively. There was a statistically significant positive correlation of 78.7% between the vitamins D values of the mothers and the infants (p: 0.000, p<0.05). Conclusion: This study conducted that a positive correlation of between the vitamin D values of the mothers and the infants. In order to prevent maternal vitamin D deficiency, the appropriate dose of prophylaxis providing optimal levels of vitamin D and should be given by according to the levels of 25 (OH) D vitamin during pregnancy.

Analytical and clinical evaluation of the new Fujirebio Lumipulse®G non-competitive assay for 25(OH)-vitamin D and three immunoassays for 25(OH)D in healthy subjects, osteoporotic patients, third trimester pregnant women, healthy African subjects, hemodialyzed and intensive care patients

2015 ◽  
Vol 0 (0) ◽  
Author(s):  
Etienne Cavalier ◽  
Pierre Lukas ◽  
Anne-Catherine Bekaert ◽  
Stéphanie Peeters ◽  
Caroline Le Goff ◽  
...  
Keyword(s):  
Vitamin D ◽  
Intensive Care ◽  
Pregnant Women ◽  
Clinical Evaluation ◽  
Healthy Subjects ◽  
Analytical Evaluation ◽  
Third Trimester ◽  
Competitive Assay ◽  
Intensive Care Patients ◽  
25 Oh Vitamin D

AbstractIn this study, we provide a short analytical evaluation of the new Fujirebio LumipulseLumipulseThe LumipulseFujirebio Lumipulse

scholarly journals Association of Colecalciferol, Ferritin, and Anemia among Pregnant Women: Result from Cohort Study on Vitamin D Status and Its Impact during Pregnancy and Childhood in Indonesia

Anemia ◽  
2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Raden Tina Dewi Judistiani ◽  
Lani Gumilang ◽  
Sefita Aryuti Nirmala ◽  
Setyorini Irianti ◽  
Deni Wirhana ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cohort Study ◽  
First Trimester ◽  
Strongly Correlated ◽  
Second Trimester ◽  
Iron Cycling ◽  
Third Trimester ◽  
Prepregnancy Body Mass Index ◽  
25 Oh Vitamin D

Studies had shown that iron-cycling was disturbed by inflammatory process through the role of hepcidin. Pregnancy is characterized by shifts of interleukin. Our objective was to determine if 25(OH) vitamin D (colecalciferol) status was associated with ferritin, anemia, and its changes during pregnancy. Method. A cohort study was done in 4 cities in West Java, Indonesia, beginning in July 2016. Subjects were followed up until third trimester. Examinations included were maternal ferritin, colecalciferol, and haemoglobin level. Result. 191 (95.5%) subjects had low colecalciferol, and 151 (75.5%) among them were at deficient state. Anemia is found in 15 (7.5%) subjects, much lower than previous report. Proportion of anemia increased by trimester among women with colecalciferol deficiency. Ferritin status and prepregnancy body mass index in the first trimester were correlated with anemia (r=0.147, p=0.038 and r=-0.56, p=0.03). Anemia in the second trimester was strongly correlated with anemia in the third trimester (r=0.676, p<0.01). Conclusion. Our study showed that the state of colecalciferol was not associated with either ferritin state or anemia, but proportion of anemia tends to increase by trimester in the colecalciferol deficient subjects.

scholarly journals Association of Systemic Sclerosis and Periodontitis with Vitamin D Levels

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 705
Author(s):  
Gaetano Isola ◽  
Giuseppe Palazzo ◽  
Alessandro Polizzi ◽  
Paolo Murabito ◽  
Clemente Giuffrida ◽  
...  
Keyword(s):  
Vitamin D ◽  
Regression Analysis ◽  
Systemic Sclerosis ◽  
Healthy Subjects ◽  
Multivariate Regression Analysis ◽  
Plaque Score ◽  
Negatively Associated ◽  
Vitamin D Levels ◽  
D Values ◽  
The Impact

The aim of the present study was to analyze the association among systemic sclerosis (SSc), periodontitis (PT); we also evaluated the impact of PT and SSc on vitamin D levels. Moreover, we tested the association with potential confounders. A total of 38 patients with SSc, 40 subjects with PT, 41 subjects with both PT and SSc, and 41 healthy controls were included in the study. The median vitamin D levels in PT subject were 19.1 (17.6–26.8) ng/mL, while SSc + PT group had vitamin d levels of 15.9 (14.7–16.9) ng/mL, significantly lower with respect to SSc patients (21.1 (15.4–22.9) ng/mL) and to healthy subjects (30.5 (28.8–32.3) ng/mL) (p < 0.001). In all subjects, vitamin D was negatively associated with c-reactive protein (CRP) (p < 0.001) and with probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), and plaque score (PI) (p < 0.001 for all parameters) and positively related to the number of teeth (p < 0.001). Moreover, univariate regression analysis demonstrated an association among high low-density lipoproteins (LDL) cholesterol (p = 0.021), CRP (p = 0.014), and PT (p < 0.001) and reduced levels of vitamin D. The multivariate regression analysis showed that PT (p = 0.011) and CRP (p = 0.031) were both predictors of vitamin D levels. Subjects with PT and SSc plus PT had significant lower vitamin D values with respect to SSc and to healthy subjects. In addition, PT seems negatively associated with levels of vitamin D in all analyzed patients.

scholarly journals AB0903 VITAMIN D LEVELS IN PRIMARY CARE AND RHEUMATOLOGY PATIENTS

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1754.2-1755
Author(s):  
H. D. Marta ◽  
A. García Dorta ◽  
C. Luis Zárate ◽  
S. Bustabad ◽  
F. Diaz-Gonzalez
Keyword(s):  
Primary Care ◽  
Vitamin D ◽  
Population Distribution ◽  
Demographic Data ◽  
Primary Care Doctor ◽  
Healthy Population ◽  
Bone Pathology ◽  
Vitamin D Levels ◽  
Vitamin D Supplements ◽  
25 Oh Vitamin D

Background:Although many studies are calling into question the benefits commonly attributed to the vitamin D out of the bone sphere, in the recent years its determination and supplementation has been generalized in the population. Causes of this trend are not clear, but generalist media pressure or even specialized over patients and doctors, along with overrated normality levels could be contributing to this fact. Actual literature123indicates that 25-OH vitamin D levels of 30ng/ml or higher are not necessary, and most of the authors agree that 20ng/ml levels are enough for the general population, and only levels below 12.5ng/ml must be considered deficient and subsidiaries of supplementation.Objectives:Obtain the vitamin D levels distribution from a sample of individuals with no bone pathology, or supplementation prescription in Tenerife’s North Area.Methods:Retrospective descriptive study of the 25-OH vitamin D levels requests from the Tenerife’s North Area, made for any reason by the Primary Care Doctor or the rheumatologist, both in the Primary Care Centers and the Hospital Universitario de Canarias (HUC). 25-OH vitamin D values were gathered from 2662 blood samples from a total of 2635 patients, from September to November of 2018 (2241 from Primary Care and 421 from rheumatology). In order to determine the use of calcium and vitamin D supplements, and the presence of bone pathology, either renal or from a malabortive process, 400 individuals were randomized (250 from primary care and 150 from rheumatology. Demographic data (age and gender), calcium serum, phosphor and 25-OH vitamin D levels were gathered for the individual records. With regards to the treatment, data about vitamin D supplements, calcium with vitamin D, or the sum of both, that the patient may have in electronic prescriptions at that time; as well as osteoporosis treatment (biphosphonates, denosumab or teriparatide) were gathered.Results:Using the age, gender, male/female relation, the levels of vitamin D, calcium and phosphor, as comparison factors; the characteristics of the random population were statistically indistinguishable from the global population. Regarding the random sample characteristics, from the 150 rheumatology patients, 11 were men (7.3%) and 139 women (92.7%). While from the 250 primary care patients, 66 were men (26.4%) and 184 were women (73.6%). The average age of the primary care sample was 55.76±19.72 years and 65.16±13.84 years in the rheumatology sample.In the total random healthy population: without bone pathology, renal or malabortive and without calcium, vitamin D or antiresorptive drug (n=181) treatment, the levels of vitamin D were 31±14ng/ml with a normal distribution and without clear differences between the primary care and rheumatology patients. When the healthy population distribution was studied by vitamin D levels, the 55% presented values below 30ng/ml, 12% below 20ng/ml and 4% showed levels under 12.5ng/ml: levels agreed as deficient (see graph).Conclusion:The 55% of the patients studied in primary care and rheumatology, without renal, digestive or bone disease and without vitamin D supplement, presented vitamin levels below the actual limits of 30ng/ml. These limits, used by most of the laboratories, tend to overestimate the vitamin D deficiency.References:[1]Manson, JE. N Engl J Med 2018, DOI: 10.1056/NEJMoa1809944.[2]Spector, TD. BMJ 2016: 355: i6183[3]Wu, F. Osteoporosis International 2017; 28: 505-515.Disclosure of Interests:None declared

Simultaneous measurement of 25(OH)-vitamin D and 24,25(OH)2-vitamin D to define cut-offs for CYP24A1 mutation and vitamin D deficiency in a population of 1200 young subjects

2020 ◽  
Vol 58 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Etienne Cavalier ◽  
Loreen Huyghebaert ◽  
Olivier Rousselle ◽  
Anne-Catherine Bekaert ◽  
Stéphanie Kovacs ◽  
...  
Keyword(s):  
Vitamin D ◽  
Young Adults ◽  
Vitamin D Deficiency ◽  
Simultaneous Measurement ◽  
Normal Population ◽  
Serum Samples ◽  
Adolescent And Young Adults ◽  
Young Subjects ◽  
25 Oh Vitamin D ◽  
D Values

AbstractBackgroundSimultaneous measurement of 25(OH)D and 24,25(OH)2D is a new tool for predicting vitamin D deficiency and allows evaluating CYP24A1 lack of function. Interpretation of 24,25(OH)2D should be performed according to 25(OH)D levels and a ratio, called the vitamin D metabolite ratio (VMR) has been proposed for such a purpose. Unfortunately, the VMR can be expressed in different ways and cannot be used if 24,25(OH)2D concentrations are undetectable. Here, we propose evaluating the enzyme activity taking into consideration the probability that a normal population presents undetectable 24,25(OH)2D concentrations according to 25(OH)D levels. We thus retrospectively measured 25(OH)D and 24,25(OH)2D in a population of 1200 young subjects to evaluate the 25(OH)D threshold above which the enzyme was induced.MethodsSerum samples from 1200 infants, children, adolescent and young adults were used to simultaneously quantify 25(OH)D and 24,25(OH)2D by LCMS/MS.ResultsMedian (interquartile range [IQR]) levels were 20.6 (14.4–27.2) ng/mL for 25(OH)D. 172 subjects (14.3%) presented 24,25(OH)2D values below the LOQ. When 25(OH)D values were <11 ng/mL, 63.1% of subjects presented undetectable 24,25(OH)2D concentrations. Percentage decreased with increasing 25(OH)D values to become 19.7% for 25(OH)D comprised between 12 and 15 ng/mL, 5.1% for 25(OH)D between 16 and 20 and 0.7% for 25(OH)D >21 ng/mL.ConclusionsWe suggest using a statistical approach to evaluate CYP24A1 function according to 25(OH)D concentrations. Our results also show that vitamin D deficiency, as defined biochemically, could be around 20 ng/mL in infants, children, adolescent and young adults and that vitamin D deficiency could be evaluated on a more individual basis.

Effects of Age and Health on the Euthyroid Reference Ranges for Serum Free Thyroxine and Free Triiodothyronine

1985 ◽  
Vol 24 (02) ◽  
pp. 57-65 ◽  
Author(s):  
J. E. M. Midgley ◽  
K. R. Gruner
Keyword(s):  
Lower Limit ◽  
Healthy Subjects ◽  
Thyroid Dysfunction ◽  
Reference Range ◽  
Free Thyroxine ◽  
Reference Ranges ◽  
Free Triiodothyronine ◽  
Serum Free ◽  
Young Subjects ◽  
Age Range

SummaryAge-related trends in serum free thyroxine (FT4) and free triiodothyronine (FT3) concentrations were measured in 7248 euthyroid subjects (age-range 3 months to 106 years). 5700 were patients referred to hospitals for investigation of suspected thyroid dysfunction, but who were diagnosed euthyroid. 1548 were healthy blood donors (age-range 18-63 years) with no indication of thyroid dysfunction. FT4 concentrations were little affected by the age, the sex or the state of health of the subjects in either group. Serum FT3 concentrations were significantly affected by both age and health factors. The upper limit of the euthyroid reference range for young subjects up to 15 years was about 20% higher (10.4 pmol/1) than for adult subjects older than 25 years (8.8 pmol/1). The change in the upper limits typical of young subjects to that typical of adults occurred steadily over the decade 15–25 years. After this age, little further change occurred, especially in healthy subjects. Additionally, the lower limit of the euthyroid range for FT3 was extended by the inclusion in the reference group of patients referred to hospitals. Compared with the lower limit of the FT3 range for healthy subjects (5 pmol/1), the corresponding limit for referred subjects (young or adult) was 3.5–3.8 pmol/1. Broadening of the FT3 reference range was probably brought about by a significant number of patients in the hospital-referred group with the “1OW-T3 syndrome” of mild non-thyroidal illness. Accordingly, FT3 was inferior to FT4 in the discrimination of hypothyroidism, as FT4 was unaffected by this phenomenon. Effects of age and non-thyroidal illness on serum FT3 concentrations require great care when selecting subjects for a laboratory euthyroid reference range typical of the routine workload. Constraints on the choice of subjects for FT4 reference ranges are less stringent.

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