scholarly journals In Vitro Biocompatibility Evaluation of Nine Dermal Fillers on L929 Cell Line

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Vincenza Cannella ◽  
Roberta Altomare ◽  
Vincenza Leonardi ◽  
Laura Russotto ◽  
Santina Di Bella ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Medical Devices ◽  
L929 Cell ◽  
Cell Number ◽  
Proliferation Rate ◽  
Two Samples ◽  
Human Use ◽  
L929 Cell Line

Objective. Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in patients following the injection. Biocompatibility assays are required for medical devices intended for human use, in order to prevent damages or injuries in the host. In this study, nine HA fillers were tested in order to evaluate their cytotoxicity and their effects on L929 cell line, according to the UNI EN ISO 10993 regulation. Methods. Extracts were prepared from nine HA fillers, and MTS viability assay was performed after 24 h, 48 h, and 72 h of exposure of cells to extracts. Cells cultured with HA filler extracts were monitored for up to 72 h, counted, and stained with haematoxylin/eosin in order to evaluate the cell proliferation rate and morphology. Results. None of the filler tested showed a cytotoxic effect. Two samples showed a higher vitality percentage and higher cell number while two samples showed a lower vitality percentage and lower cell number at 72 h. Conclusion. Data obtained suggest that although examined fillers are not cytotoxic, they show different effects on the in vitro cell proliferation rate. In vitro studies of medical devices could lead to important implications since these could aid to predict effects about their in vivo application. These easy and rapid assays could be useful to test new materials intended for human use avoiding animal tests.

In vitro cytotoxic effects of DEHP-alternative plasticizers and their primary metabolites on a L929 cell line

Chemosphere ◽  
2017 ◽  
Vol 173 ◽  
pp. 452-459 ◽  
Author(s):  
Teuta Eljezi ◽  
Pierre Pinta ◽  
Damien Richard ◽  
Jérémy Pinguet ◽  
Jean-Michel Chezal ◽  
...  
Keyword(s):  
Cell Line ◽  
L929 Cell ◽  
Cytotoxic Effects ◽  
Primary Metabolites ◽  
L929 Cell Line

scholarly journals Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study

2020 ◽  
Vol 22 (3) ◽  
Author(s):  
Maria Szymonowicz ◽  
_ Agnieszka ◽  
Magdalena Pajączkowska ◽  
Joanna Nowicka ◽  
Kamila Wiśniewska ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Cell Line ◽  
Mtt Assay ◽  
In Vitro Study ◽  
L929 Cell ◽  
Aluminium Chloride ◽  
Srb Assay ◽  
Haemostatic Agents ◽  
L929 Cell Line

Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.

Epinephrine Stimulates Cell Proliferation and Induces Chemoresistance in Myeloma Cells through the β-Adrenoreceptor in vitro

2017 ◽  
Vol 138 (2) ◽  
pp. 103-110 ◽  
Author(s):  
Yang Liu ◽  
Xiaochen Yu ◽  
Junling Zhuang
Keyword(s):  
Cell Proliferation ◽  
Cell Growth ◽  
Cell Line ◽  
Receptor Subtype ◽  
Dependent Manner ◽  
Myeloma Cells ◽  
U266 Cell ◽  
Β Receptor ◽  
U266 Cell Line

Objectives: To explore the effect of the β-adrenoreceptor signaling pathway on myeloma cells. Methods: The myeloma U266 cell line was treated with epinephrine and propranolol. Cell proliferation was analyzed by MTS assay. Apoptosis was detected by flow cytometry. The β-receptor subtype and the key enzyme of epinephrine were identified by reverse transcription polymerase chain reaction (RT-PCR). Results: Epinephrine (5-50 μM) promoted U266 cell growth in a dose-dependent manner and neutralized the inhibition effect of bortezomib (25 and 50 ng/mL) in vitro. Cell proliferation was inhibited by a β-receptor antagonist, propranolol, at a concentration of 50-200 μM. The proportions of early and late apoptotic cells were enhanced after treatment with propranolol. The expression of caspase 3/7, 8, and 9 was elevated in propranolol-treated myeloma cells. Both β1- and β2-adrenoceptor mRNAs were expressed in the U266 cell line. Key enzymes dopamine hydroxylase and tyrosinehydroxylase were identified in myeloma cells. Conclusions: Our results reveal that epinephrine stimulates myeloma cell growth in vitro while the β-blocker propranolol has an antiproliferative effect, indicating that stress hormones may trigger the progression of myeloma.

Differential effects of epidermal growth factor (EGF) on cell locomotion and cell proliferation in a cloned human embryonal carcinoma-derived cell line in vitro

1986 ◽  
Vol 86 (1) ◽  
pp. 47-55
Author(s):  
W. Engstrom
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Cell Line ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Colony Diameter ◽  
Cell Locomotion ◽  
Epidermal Growth

The effects of epidermal growth factor (EGF) on clones from a human embryonal carcinoma-derived cell line (Tera-2) have been studied. Cells were plated at clonal densities, whereafter the effects of serum and EGF on cell locomotion and cell proliferation were examined. The addition of 50 ngEGF ml-1 resulted in increased migration, as judged by increased colony diameter in the presence of EGF. However, the effect of EGF on cell locomotion was rarely accompanied by any effect on cell proliferation. It was concluded that EGF exerts a preferential effect on cell migration in human embryonal carcinoma cells in vitro.

Characterization and In Vitro Toxicity of French Process Zinc Oxide Nanoparticles with High Surficial Zinc

2019 ◽  
Vol 290 ◽  
pp. 274-279
Author(s):  
Sufiniza Nordin ◽  
Shahrom Mahmud ◽  
Azman Seeni ◽  
Nur Mariam Kamaruddin ◽  
Nur Syuhada Ahmad
Keyword(s):  
L929 Cell ◽  
Average Crystallite Size ◽  
Fibroblast Cell ◽  
In Vitro Toxicity ◽  
Visible Absorption ◽  
Zno Nanopowder ◽  
L929 Fibroblast ◽  
Calculated Average ◽  
L929 Cell Line

In this study, we investigated in vitro toxicity of ZnO nanopowder on L929 fibroblast cell lines. The ZnO nanoparticles were observed to possess relatively more surficial zinc compared to oxygen. Field-emission scanning electron microscope (FESEM) data revealed that the particle morphologies consisted of nanorods, platelets and nodules between 40-100 nm size range. EDS confirmed that there were more zinc elements on the surfaces of the particles. XRD results showed that the calculated average crystallite size of ZnO nanopowder was 44.28 nm. The optical band gap calculated was 3.298 eV based on UV-visible absorption spectra. In vitro toxicity results showed that ZnO concentration at 0.3125mM, 0.625mM and 1.25 mM were considered non-toxic to L929 cell line since the cell viability was higher than 70 % after 72 hours treatment whereas the ZnO nanopowder concentration above 2.5mM was considered toxic. High surficial zinc atoms on ZnO particles could have been a significant factor in cell toxicity.

PKC412 Shows Strong Anti-myeloma effects in in-Vitro-Studies

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5172-5172
Author(s):  
Ahmet H Elmaagacli ◽  
Michael Koldehoff ◽  
Nina K Steckel ◽  
Dietrich Beelen
Keyword(s):  
Multiple Myeloma ◽  
Mrna Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Interleukin 6 ◽  
Proliferation Rate ◽  
In Vitro Studies ◽  
Apoptosis Rate ◽  
Rpmi 8226

Abstract Background. The protein kinase C (PKC) inhibitor PKC412 (N-benzylstaurosporine) is a derivate of the naturally occurring alkaloid staurosproine and has been shown to inhibit the conventional isoforms of PKC (alfa, beta1, beta2 and gamma). PKC412 has been shown to have an antitumor effect on non-small cell lung cancer and acute leukemia with FLT3 mutations, but little is known about its effect on multiple myeloma up to date. Methods. Since PKC is also an inhibitor of a tyrosin kinase which is associated with VEGF, and inhibits the release of Interleukin-6, TNF alfa, and that of growth factor dependent C-FOS, we postulated that PKC412 might have also strong anti-myeloma features. Here we evaluated the anti-myeloma effect of PKC412 in the multiple myeloma cell lines INA-6, OPM-2 and RPMI 8226 by measuring its effect on their proliferation rate, the apoptosis rate and the Interleukin-6 mRNA expression. Results. PKC412 showed strong anti-myeloma effects in all three celllines. 50nM of PKC412 was enough to drop the proliferation rate in all three cell lines under 10% compared to untreated cells(p<0.01). The apoptosis rate increased in INA cell line up to 2,5 times and in RPMI cell line up to 3 times (p<0.05), whereas only a moderate increase was observed in the OPM2 cell line with 500nM of PKC412. As expected, the IL-6 mRNA expression decreased after PKC412 treatment in all three cell lines more than 50%. The addition of Bevacizumab to PKC412 in RPMI and OPM-2 cell lines did not increased the apoptosis rate significantly, whereas the addition of short-interference RNA (RNAi) against VEGF increased the apoptosis rate in RPMI 8226 cells about 20% (p<0.05) and in OPM-2 cells up to 30% (p<0.01) compared to PKC412 alone, which was also associated concordantly with a further reduction of the proliferation rate in RPMI and OPM-2 cells up to 30%. Conclusions. PKC412 shows strong anti-myeloma effects and might be effective also in the treatment of patients with multiple myeloma. These in-vitro studies might encourage to initiate clinical trials with PKC412 in patients with multiple myeloma.

Effects of Phthalates on the Human Corneal Endothelial Cell Line B4G12

2012 ◽  
Vol 31 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Tanja Krüger ◽  
Yi Cao ◽  
Søren K. Kjærgaard ◽  
Lisbeth E. Knudsen ◽  
Eva C. Bonefeld-Jørgensen
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Endothelial Cell ◽  
Cell Line ◽  
Corneal Endothelial Cell ◽  
Industrial Chemicals ◽  
Cell Secretion ◽  
Endothelial Cell Line ◽  
Butyl Phthalate

Phthalates are industrial chemicals used in many cosmetics. We evaluated an in vitro model for eye irritancy testing using the human corneal endothelial cell line B4G12. Cell proliferation and toxicity were assessed after exposing to di- n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethylhexyl phthalate (DEHP), diisodecyl phthalate (DIDP), di- n-octyl phthalate (DnOP), and di-isononyl phthalate (DINP). Gene expression and secretion of inflammatory cytokines were evaluated after exposure to DBP. Decreased cell proliferation was observed for the phthalates DBP, BBP, and DEHP, and cell toxicity was observed for DBP and BBP. Upon DBP exposure at nontoxic concentrations, a significant increased gene expression and cytokine cell secretion were observed for interleukin-1β (IL-1β) and IL-8, and also an increased IL-6 secretion was observed. In conclusion, the human corneal endothelial cell line B4G12 may be a potential model for inflammatory eye irritancy testing of phthalates.

scholarly journals Cyclic stretch attenuates effects of hyperoxia on cell proliferation and viability in human alveolar epithelial cells

2006 ◽  
Vol 291 (2) ◽  
pp. L166-L174 ◽  
Author(s):  
Ryan M. McAdams ◽  
Shamimunisa B. Mustafa ◽  
Jeffrey S. Shenberger ◽  
Patricia S. Dixon ◽  
Barbara M. Henson ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Cell Injury ◽  
A549 Cells ◽  
Cell Number ◽  
Alveolar Epithelial Cells ◽  
Cyclic Stretch ◽  
Pulmonary Epithelium ◽  
Alveolar Epithelial

The treatment of severe lung disease often requires the use of high concentrations of oxygen coupled with the need for assisted ventilation, potentially exposing the pulmonary epithelium to both reactive oxygen species and nonphysiological cyclic stretch. Whereas prolonged hyperoxia is known to cause increased cell injury, cyclic stretch may result in either cell proliferation or injury depending on the pattern and degree of exposure to mechanical deformation. How hyperoxia and cyclic stretch interact to affect the pulmonary epithelium in vitro has not been previously investigated. This study was performed using human alveolar epithelial A549 cells to explore the combined effects of cyclic stretch and hyperoxia on cell proliferation and viability. Under room air conditions, cyclic stretch did not alter cell viability at any time point and increased cell number after 48 h compared with unstretched controls. After exposure to prolonged hyperoxia, cell number and [3H]thymidine incorporation markedly decreased, whereas evidence of oxidative stress and nonapoptotic cell death increased. The combination of cyclic stretch with hyperoxia significantly mitigated the negative effects of prolonged hyperoxia alone on measures of cell proliferation and viability. In addition, cyclic stretch resulted in decreased levels of oxidative stress over time in hyperoxia-exposed cells. Our results suggest that cyclic stretch, as applied in this study, can minimize the detrimental effects of hyperoxia on alveolar epithelial A549 cells.

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