scholarly journals Nanoencapsulation of Chromolaena odorata Extract Using Pluronic F127 as an Effectively Herbal Delivery System for Wound Healing

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Ngoc-Dung Huynh Luu ◽  
Le Hang Dang ◽  
Hoang Minh Bui ◽  
Trang Thuy Thi Nguyen ◽  
Bich Tram Nguyen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Clot ◽  
Fibroblast Cell ◽  
Ethyl Acetate Fraction ◽  
Negative Control ◽  
Pluronic F127 ◽  
Chromolaena Odorata ◽  
Long Term Storage ◽  
Copolymer Micelles

Chromolaena odorata is a medicinal herb with prominent pharmacological properties. The therapeutic efficiency of Chromolaena odorata extracts and its ingredients have, however, been limited by various factors, including the lack of targeting capacity and poor bioavailability. To approach this drawback, ethyl acetate fraction extract of Chromolaena odorata- (EA.ChO-) encapsulated pluronic-based nanocarriers was disclosed herein. The most common pluronic triblock copolymer micelles (pluronic F127) was used for the nanosized formulation of Chromolaena odorata extract. The obtained results show that EA.ChO-encapsulated nanoparticles have a spherical morphology with a designed hydrodynamic size was about 183.7 nm and zeta potential -39.5 mV. The EA.ChO nanoparticles are stable in different aqueous solutions (water, PBS 2.8, and PBS 7.4). The lyophilized form of the EA.ChO nanoparticles exhibited excellent stability for long-term storage. Notably, the EA.ChO nanoparticles were 1.3-1.4 fold more effective in the growth of fibroblast than the free EA.ChO, verifying the potential of pluronic F127 nanoparticles to the increased function of EA.ChO in the proliferation of fibroblast cell. In addition, bleeding stopped within 55 ± 6  s which was 20 s faster than that of free EA.ChO and 38-44 s faster than that of negative control treatments. The EA.ChO nanoencapsulation processed a rapid blood clot formation compared to control, free EA.ChO, pluronic F127, and water, suggesting the excellent bioavailability of EA.ChO nanoencapsulation. The obtained results thus provided a promising prospect for raising the activity Chromolaena odorata extract in wound healing application.

scholarly journals Hemostatic and Wound Healing Properties of Chromolaena odorata Leaf Extract

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Hataichanok Pandith ◽  
Xiaobo Zhang ◽  
Jason Liggett ◽  
Kyung-Won Min ◽  
Wandee Gritsanapan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Molecular Mechanisms ◽  
Fibroblast Cell ◽  
Luciferase Assay ◽  
Heme Oxygenase 1 ◽  
Dependent Manner ◽  
Chromolaena Odorata ◽  
Expression Of Genes ◽  
Potent Vasoconstrictor ◽  
Cell Migration And Proliferation

Chromolaena odorata (L.) King and Robinson (Siam weed) extract has been used to stop bleeding and in wound healing in many tropical countries. However, its detailed mechanisms have not been elucidated. In this study, we examined the molecular mechanisms by which Siam weed extract (SWE) affected hemostatic and wound healing activities. SWE promoted Balb/c 3T3 fibroblast cell migration and proliferation. Subsequently, we found that heme oxygenase-1 (HO-1), the accelerating wound healing enzyme, was increased at the transcriptional and translational levels by SWE treatments. The HO-1 promoter analyzed with luciferase assay was also increased by treatment of SWE in a dose-dependent manner. This induction may be mediated by several kinase pathways including MEK, p38MAPK, AKT, and JNK. Quantitative real-time PCR using undifferentiated promonocytic cell lines revealed that thromboxane synthase (TXS), a potent vasoconstrictor and platelet aggregator, was increased and MMP-9, an anti platelet aggregator, was decreased in the presence of SWE. Our studies presented that SWE accelerated hemostatic and wound healing activities by altering the expression of genes, including HO-1, TXS, and MMP-9.

scholarly journals Effects of Wound-Healing Management on Potato Post-Harvest Storability

Agronomy ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 512 ◽  
Author(s):  
Yi Wang ◽  
Mack R. Naber ◽  
Trevor W. Crosby
Keyword(s):  
Weight Loss ◽  
Wound Healing ◽  
Potato Storage ◽  
Storage Quality ◽  
Standard Practice ◽  
Post Harvest ◽  
Long Term Storage ◽  
Term Storage

A critical step in profitable post-harvest potato storage management is to cure tubers at appropriate temperatures long enough for rapid wound-healing to prevent disease and defect development, but not too long to jeopardize storage quality. A two-year storage study was conducted in macro-storage totes at the University of Wisconsin Hancock storage research facility to evaluate the effects of higher wound-healing temperatures (15.6 °C, 18.3 °C) imposed for different durations, and compare them to the U.S. potato industry’s standard practice (12.8 °C), on weight loss and frying quality of multiple processing potato varieties during long-term storage. It was found that in the experimental setting of this study, warmer wound-healing temperatures resulted in (1) less weight loss, particularly during the early storage season across varieties; (2) ameliorated senescent sweetening of the Snowden variety; (3) improved fry quality of the Russet Burbank variety; (4) and no apparent disease spread during long-term storage if tubers were harvested healthy out of fields. Overall, no significant difference was found between 15.6 °C and 18.3 °C regarding their treatment effects. Our conclusion is that compared to the current standard practice, higher wound-healing temperatures may have the potential benefits of improving potato storage quality while reducing the economic penalty associated with weight loss for specific varieties, but tubers should be healthy at harvest in order to gain the benefits. Further research is needed to test if those benefits of higher wound-healing temperatures hold true in large-scale commercial storage facilities.

scholarly journals Effect of Different Silane Treatments on Long-Term Bonding Between Non-Etched Glass-Ceramic and Resin Cement

2017 ◽  
Vol 19 (2) ◽  
pp. 33 ◽  
Author(s):  
Fabián Murillo Gómez DDS, MSc ◽  
Mario F. De Goes DDS, MSc, PhD
Keyword(s):  
Glass Ceramic ◽  
Ceramic Materials ◽  
Positive Control ◽  
Negative Control ◽  
Resin Cement ◽  
Ceramic Surface ◽  
Cohesive Failure ◽  
Universal Adhesive ◽  
Long Term Storage

The present study evaluated the bond strength between glass-ceramic and resin cement, using different silane treatments, performing no previous hydrofluoric acid (HF) etching on ceramic surface, after short- and long- term storage. One hundred and eighty glass-ceramic plaques (IPS e.max CAD®) were polished and divided into six groups (n=30) to receive different silane treatments: (1) RCP-RelyX Ceramic Primer® (one-bottle silane), (2) RCP+SB-RelyX Ceramic Primer and Adper Singlebond2® (silane plus separated adhesive), (3) SBU-Scotchbond Universal® (silane-containing universal adhesive), (4) CP-Clearfil Ceramic Primer® (silane/MDP primer), (5) NC-no-silane (negative control) and (6) PC-Previous HF etching (5%, 20s) plus RelyX Ceramic Primer® (positive control). Two resin cement cylinders (Rely X Ultimate®) were built on each plaque. Each group was divided into two sub-groups to be stored for 24 hours (24h) or 6 months (6mo) in distilled water at 37°C (n=15). Then microshear (µSBS) testing was performed. Failure mode was analyzed using scanning electron microscopy (SEM). Data were statistically analyzed by two-way ANOVA and Tukey´s test (α=0.05). Both factors and their interaction resulted statistically significant (p≤0.05). PC obtained the highest µSBS values (in MPa) at both storage times (24h: 28.11±2.44; 6mo: 19.10±3.85). After 24h storage, groups RCP+SB (10.86±3.62), SBU (8.37±4.33) and CP (8.05±3.62) were not statistically different from NC (8.00±2.51); only RCP (19.73±4.63) and PC obtained higher values. After 6 months, only PC showed higher values than NC (0.04±0.01). Cohesive failure in resin cement was more prevalent for RCP-24h and PC, while adhesive failure was more frequent among all other groups.  Clinical relevance: None of the commercially available silane primers tested, improve long- term ceramic/cement bonding without performing HF etching on ceramic surface. Combination of HF acid and silane, remain as a gold standard treatment for glass-ceramic materials. 

scholarly journals Gambaran Penyembuhan Luka Tikus Diabetes Dengan Fraksi Etil Asetat Daun Binahong (Anredera cordifolia (Tenore) Steenis)

2018 ◽  
Vol 1 (3) ◽  
pp. 158-161
Author(s):  
Trie Yuni Elfasyari ◽  
Kintoko Kintoko ◽  
Nurkhasanah Nurkhasanah
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Healing Process ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Punch Biopsy ◽  
Negative Control ◽  
Diabetic Rat ◽  
Wound Healing Process ◽  
Wound Healing Activity

Proses penyembuhan luka kulit dipengaruhi oleh kondisi dibetes mellitus (DM). Ekstrak etanol daun binahong dilaporkan dapat mempercepat penyembuhan luka tikus diabetes melalui mekanisme reepitelisasi dan kolagenasi. Penelitian ini dilakukan untuk mengetahui gambaran penyembuhan luka diabetes fraksi etil asetat daun binahong (Anredera cordifolia (Ten.) Steenis) konsentrasi 10% dengan parameter jumlah sel fibroblast pada tikus yang diinduksi sterptozotocin. Metode fraksinasi yang digunakan fraksinasi bertingkat padat cair dengan pelarut etil asetat yang bersifat semipolar. Penelitian eksperimental menggunakan 20 ekor tikus Wistar jantan usia 2 bulan, berat badan antara 150-200 gram yang diinduksi streptozotocin (STZ) dosis tunggal 45 mg/kgBB i.p. Punch biopsy digunakan untuk membuat luka pada kulit tikus dengan diameter 5 mm setelah kadar glukosa darah (KGD) di atas 250 mg/dL. Hewan uji dibagi menjadi 4 kelompok, terdiri dari kontrol sehat (tanpa induksi STZ dan tanpa perlakuan), kontrol negatif (biocream), kontrol positif (madecassol), dan fraksi etil asetat daun binahong 10% (dalam biocream). Aplikasi topikal dilakukan 2 kali sehari sebanyak 25 mg selama 10 hari pada area luka dan sekitarnya. Pengamatan dilakukan secara makroskopis dan mikroskopis. Penelitian menunjukkan bahwa fraksi etil asetat daun binahong mengandung senyawa flavonoi dan tanin, serta berperan dalam meningkatkan jumlah sel fibroblast (P<0.05).   Wound healing process is affected by diabetes melitus condition. The ethanol extract of Binahong leaves has reported to have wound healing activity on diabetic rat through mechanism of re-epithelialization and collagenation. This study was conducted to evaluate the wound healing activity on diabetic rat of ethyl acetate fraction of  Binahong (Anredera cordifolia (Tenore) Steenis) leaves at concentration of 10% with parameters of fibroblast numbers in streptozozin induced rat. The fractionation method used was solid-liquid graded fractination using ethyl acetate as semipolar solvent. This experimental research used 20 male wistar rats, aged 2 months, weight 150-200 g, induced by single dose streptozotocin (STZ)45 mg/kgBB i.p. Punch biopsy was used to injured the rat skin with diameter of 5 mm after blood glucose level was more than 250 mg/dL. Test animals were divided into 4 groups, these include healthy control (without induced by STZ and treatment), negative control (biocream), positive control (madecassol), ethyl acetate fraction of binahong leaves 10% (in biocream). The cream was applied topically 2 times a day with an amount of 25 mg for 10 days on wound area and surrounding. The macroscopic and microscopic observation was performed. The results showed that ethyl acetate fraction of binahong leaves contained flavonoids and tannin, besides it plays role to increase the fibroblast numbers  (P<0.05).

scholarly journals UJI EFEKTIVITAS SEDIAAN GEL FRAKSI ETIL ASETAT DAUN JAMBU BIJI (Psidium guajava Linn) TERHADAP PENYEMBUHAN LUKA TERBUKA PADA MENCIT (Mus musculus)

2016 ◽  
Vol 16 (2) ◽  
pp. 23-32
Author(s):  
Lydia Septa Desiyana ◽  
Muhammad Ali Husni ◽  
Seila Zhafira
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Mus Musculus ◽  
Healing Process ◽  
Ethyl Acetate Fraction ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Wound Healing Process ◽  
Guava Leaf

Guava leaf has various benefits, one of them is accelerating the healing process of a wound. This study aims to formulate the ethyl acetate fraction into a gel form and test the effectiveness of gel formulation toward open wounds in 16 male mices (Mus musculus) which made excision 1 cm circular full thickness wound on the back of mice parallel with Os. vetebra. The ethyl acetate fraction of guava leaf has been made into concentrations of 5% and 7% with base gel used as negative control and Bioplacenton as positive control. The evaluation towards ethyl acetate fraction gels showed the gels have green-yellow color, guava odor, homogenous, relevant pH to the skin 6,31-6,51, and spread over 2,8-3 cm (stiff gel). The gels were applied onto the wounds twice a day for about 21 days. The observation was conducted every day towards the scab formation day, scab chipped day, and wound healing day. The data were analyzed statistically using one way ANOVA then continued with Tukey test. The average day for scab formation, scab chipped, and wound healing process, successively are in the negative control i.e. 6,50 ; 13,50 ; 20,25, then positive control of  3,75 ; 11,00 ; 17,25, for gel with 5% concentration,  3,75 ; 9,75 ; 16,50, and gel with 7% concentration,  4,50 ; 12,00 ; 18,25. The statistical result showed that the ethyl acetats fraction  could accelerate the scab formation (p0,05) and the open-wound healing (p0,05) in mice than the control negative (p0,05), but not in the scab exfoliation (p0,05). Gel concentration 5% accelerated faster in forming the scab, flaking off the scab, and  healing the wound compared with the gel concentration of 7%. 

Preparation And elemental analysis of ancient fibers

1987 ◽  
Vol 45 ◽  
pp. 410-411
Author(s):  
Allen Angel ◽  
Kathryn A. Jakes
Keyword(s):  
Cross Sections ◽  
Physical Structure ◽  
Energy Dispersive ◽  
Archaeological Sites ◽  
X Ray ◽  
Long Term Storage ◽  
Backscattered Electron ◽  
Electron Imaging

Fabrics recovered from archaeological sites often are so badly degraded that fiber identification based on physical morphology is difficult. Although diagenetic changes may be viewed as destructive to factors necessary for the discernment of fiber information, changes occurring during any stage of a fiber's lifetime leave a record within the fiber's chemical and physical structure. These alterations may offer valuable clues to understanding the conditions of the fiber's growth, fiber preparation and fabric processing technology and conditions of burial or long term storage (1).Energy dispersive spectrometry has been reported to be suitable for determination of mordant treatment on historic fibers (2,3) and has been used to characterize metal wrapping of combination yarns (4,5). In this study, a technique is developed which provides fractured cross sections of fibers for x-ray analysis and elemental mapping. In addition, backscattered electron imaging (BSI) and energy dispersive x-ray microanalysis (EDS) are utilized to correlate elements to their distribution in fibers.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
Sign in / Sign up

Export Citation Format

Share Document