Recent Progress in Electrochemical Detection of Human Papillomavirus (HPV) via Graphene-Based Nanosensors

In the present study, applications of advanced nanomaterials such as graphene oxide (GO), reduced graphene oxide (rGO), and graphene quantum dots (GQDs) as leading and potential candidates toward treating the human papillomaviruses (HPVs) were investigated. In this matter, a comprehensive summary of the formation of GO, rGO, and GQDs will be reported in detail. Continuous efforts have been exerted to develop high-performance biosensors and electrochemical detection systems toward accurate detection of HPV using novel routes. This review paper showed that HPVs appeared in different types and species. These HPVs have many complications on humans, and thus, there are different ways that a person could be exposed to them. Meanwhile, several routes of transmitting the HPVs to human cells are discussed too. Indeed, sexually transmitted diseases could be easily infected by HPVs, although the human immune system can also be boosted to treat this dangerous virus effectively. Some of the HPVs such as HPV-16 and HPV-18 are so dangerous, and HPV DNA could be detected in several vertical cancers. Herein, we reported and summarized some recent progress in electrochemical detection of HPVs using graphene-based nanosensors.

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Sensitive electrochemical detection of nitric oxide based on AuPt and reduced graphene oxide nanocomposites

The Analyst ◽

10.1039/c6an00429f ◽

2016 ◽

Vol 141 (13) ◽

pp. 4074-4083 ◽

Cited By ~ 21

Author(s):

Zhonggang Liu ◽

Heidi Forsyth ◽

Neelam Khaper ◽

Aicheng Chen

Keyword(s):

Nitric Oxide ◽

Graphene Oxide ◽

Electrochemical Sensor ◽

Reduced Graphene Oxide ◽

Electrochemical Detection ◽

High Performance ◽

Reduced Graphene

A high-performance electrochemical sensor with AuPt nanoparticles and reduced graphene oxide nanocomposites is demonstrated for the effective detection of NO.

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Recent Advances in Graphene-Based Humidity Sensors

Nanomaterials ◽

10.3390/nano9030422 ◽

2019 ◽

Vol 9 (3) ◽

pp. 422 ◽

Cited By ~ 26

Author(s):

Chao Lv ◽

Cun Hu ◽

Junhong Luo ◽

Shuai Liu ◽

Yan Qiao ◽

...

Keyword(s):

Graphene Oxide ◽

High Performance ◽

Graphene Quantum Dots ◽

Pristine Graphene ◽

High Electron ◽

Humidity Sensors ◽

Industrial Processing ◽

Surface Areas ◽

Recent Advances ◽

Metal Metal

Humidity sensors are a common, but important type of sensors in our daily life and industrial processing. Graphene and graphene-based materials have shown great potential for detecting humidity due to their ultrahigh specific surface areas, extremely high electron mobility at room temperature, and low electrical noise due to the quality of its crystal lattice and its very high electrical conductivity. However, there are still no specific reviews on the progresses of graphene-based humidity sensors. This review focuses on the recent advances in graphene-based humidity sensors, starting from an introduction on the preparation and properties of graphene materials and the sensing mechanisms of seven types of commonly studied graphene-based humidity sensors, and mainly summarizes the recent advances in the preparation and performance of humidity sensors based on pristine graphene, graphene oxide, reduced graphene oxide, graphene quantum dots, and a wide variety of graphene based composite materials, including chemical modification, polymer, metal, metal oxide, and other 2D materials. The remaining challenges along with future trends in high-performance graphene-based humidity sensors are also discussed.

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A graphene oxide coated gold nanostar based sensing platform for ultrasensitive electrochemical detection of circulating tumor DNA

Analytical Methods ◽

10.1039/c9ay01620a ◽

2020 ◽

Vol 12 (4) ◽

pp. 440-447 ◽

Cited By ~ 4

Author(s):

Mahbubur Rahman ◽

Daxiang Cui ◽

Shukui Zhou ◽

Amin Zhang ◽

Di Chen

Keyword(s):

Graphene Oxide ◽

Gastric Carcinoma ◽

Electrochemical Reduction ◽

Electrochemical Detection ◽

Peripheral Blood ◽

High Performance ◽

Circulating Tumor Dna ◽

Electrochemical Sensing ◽

Sensing Platform ◽

Tumor Dna

A high-performance electrochemical sensing platform inspired by a functional ‘green’ electrochemical reduction pathway was developed to identify and detect circulating tumor DNA (ctDNA) of gastric carcinoma in peripheral blood.

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The Epidemiology of Human Papillomavirus Infection and Cervical Cancer

Disease Markers ◽

10.1155/2007/914823 ◽

2007 ◽

Vol 23 (4) ◽

pp. 213-227 ◽

Cited By ~ 181

Author(s):

F. Xavier Bosch ◽

Silvia de Sanjosé

Keyword(s):

Cervical Cancer ◽

Invasive Cervical Cancer ◽

Intraepithelial Neoplasia ◽

Hpv Infection ◽

Human Papillomaviruses ◽

Transmitted Infection ◽

Hpv Dna ◽

Sexually Transmitted ◽

Papillomavirus Infection ◽

Simplex Virus

Cervical cancer has been recognized as a rare outcome of a common Sexually Transmitted Infection (STI). The etiologic association is restricted to a limited number of viral types of the family of the Human Papillomaviruses (HPVs). The association is causal in nature and under optimal testing systems, HPV DNA can be identified in all specimens of invasive cervical cancer. As a consequence, it has been claimed that HPV infection is a necessary cause of cervical cancer. The evidence is consistent worldwide and implies both the Squamous Cell Carcinomas (SCC), the adenocarcinomas and the vast majority (i.e. > 95%) of the immediate precursors, namely High Grade Squamous Intraepithelial Lesions (HSIL)/Cervical Intraepithelial Neoplasia 3 (CIN3)/Carcinomain situ. Co-factors that modify the risk among HPV DNA positive women include the use of oral contraceptives (OC) for five or more years, smoking, high parity (five or more full term pregnancies) and previous exposure to other sexually transmitted diseases such as Chlamydia Trachomatis (CT) and Herpes Simplex Virus type 2 (HSV-2). Women exposed to the Human Immunodeficiency Virus (HIV) are at high risk for HPV infection, HPV DNA persistency and progression of HPV lesions to cervical cancer.

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3-Dimensional ordered reduced graphene oxide embedded with N-doped graphene quantum dots for high performance supercapacitors

Electrochimica Acta ◽

10.1016/j.electacta.2020.137018 ◽

2020 ◽

Vol 361 ◽

pp. 137018

Author(s):

Thi Ai Ngoc Bui ◽

Thuy Giang Nguyen ◽

Win Darmanto ◽

Ruey-An Doong

Keyword(s):

Quantum Dots ◽

Graphene Oxide ◽

Reduced Graphene Oxide ◽

High Performance ◽

Graphene Quantum Dots ◽

3 Dimensional ◽

Reduced Graphene ◽

Doped Graphene

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Detection of Human Papillomaviruses DNA in paired peripheral blood and cervix samples patients with cervical lesions and healthy individuals

10.1101/2021.05.29.20161125 ◽

2021 ◽

Author(s):

Kamylla Conceicao Gomes Nascimento ◽

Elyda Goncalves Lima ◽

Barbara Simas Chagas ◽

Zhilbelly Mota Nunes ◽

Marconi Rego Barros Junior ◽

...

Keyword(s):

Peripheral Blood ◽

Intraepithelial Neoplasia ◽

Human Papillomaviruses ◽

Healthy Individuals ◽

Cervical Lesions ◽

Hpv16 E6 ◽

Hpv Dna ◽

Sexually Transmitted ◽

Standard Procedures ◽

Hpv Types

This study evaluated the presence of HPV DNA in the cervix and peripheral blood of women with cervical intraepithelial neoplasia (CIN I, II, and III) and healthy individuals. Overall, 139 paired peripheral blood and cervix samples of healthy women and women with CIN I, II, and III (n= 68) were tested for HPV DNA by using standard procedures. PCR-sequencing determined HPV types. Quantification of HPV16 E6 and E2 genes was performed to determine viral load and physical state. HPV DNA was detected in the cervix (21.1% in healthy individuals; 48.8-55.5% in CIN patients), blood (46.4% in healthy individuals; 44.1-77.7% in CIN patients), and paired peripheral blood and cervix samples (24% in healthy individuals; 32.5-44.4% in CIN patients). The most frequent types found in the cervix were HPV16, 18, 31, 33, 58, and 70, while HPV16, 18, 33, 58, and 66 were the most frequent types found in the blood. HPV DNA in the cervix was associated with previous sexually transmitted infections (STIs) (P=0.023; OR: 2.978; CI:1.34-7.821), HPV DNA in the blood (P=0.000; OR: 3.369; CI:3.700-18.540), and cervical lesions (CIN I/II or III) (P=0.001; OR: 3.369; CI:1.634-6.945). Binomial Logistic regression showed that HPV DNA in the blood (P=0.000; OR: 9.324; CI:3.612-24.072) and cervical lesions (P=0.011; OR: 3.622; CI:1.338-9.806) were associated with HPV DNA in the cervix. However, we did not find an association between HPV DNA in blood and cervical lesions (P=0.385). Our results showed that, although there is an association between HPV DNA in the cervix with HPV DNA in blood, only HPV DNA found in the cervix was associated with cervical lesions.

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