scholarly journals Gut Microbiota Profile in Adult Patients with Idiopathic Nephrotic Syndrome

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Hanchang He ◽  
Minwa Lin ◽  
Lu You ◽  
Tongqing Chen ◽  
Zijie Liang ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Idiopathic Nephrotic Syndrome ◽  
Sensu Stricto ◽  
Adult Patients ◽  
Clinical Parameters ◽  
Fecal Microbiome ◽  
Nitrogen Levels ◽  
Stool Samples

Background. Increasing evidences have reported gut microbiota dysbiosis in many diseases, including chronic kidney disease and pediatric idiopathic nephrotic syndrome (INS). There is lack evidence of intestinal microbiota dysbiosis in adults with INS, however. Here, we to address the association between the gut microbiome and INS. Methods. Stool samples of 35 adult INS patients and 35 healthy volunteers were collected. Total bacterial DNA was extracted, and the V4 regions of the bacterial 16S ribosomal RNA gene were sequenced. The fecal microbiome was analyzed using bioinformatics. The correlation analysis between altered taxa and clinical parameters was also included. Results. We found that microbial diversity in the gut was reduced in adult patients with INS. Acidobacteria, Negativicutes, Selenomonadales, Veillonellaceae, Clostridiaceae, Dialister, Rombousia, Ruminiclostridium, Lachnospira, Alloprevotella, Clostridium sensu stricto, Megamonas, and Phascolarctobacterium were significantly reduced, while Pasteurellales, Parabacteroides, Bilophila, Enterococcus, Eubacterium ventriosum, and Lachnoclostridium were markedly increased in patients with INS. In addition, Burkholderiales, Alcaligenaceae, and Barnesiella were negatively correlated with serum creatinine. Blood urea nitrogen levels were positively correlated with Christensenellaceae, Bacteroidales_S24.7, Ruminococcaceae, Ruminococcus, and Lachnospiraceae_NK4A136, but were negatively correlated with Flavonifractor_plautii and Erysipelatoclostridium_ramosum. Enterobacteriales, Enterobacteriaceae, Porphyromonadaceae, Escherichia/Shigella, Parabacteroides, and Escherichia_coli were positively correlated with albumin. Proteinuria was positively correlated with Verrucomicrobia, Coriobacteriia, Thermoleophilia, Ignavibacteria, Coriobacteriales, Nitrosomonadales, Coriobacteriaceae, and Blautia, but was negatively correlated with Betaproteobacteria, Burkholderiales, and Alcaligenaceae. Conclusion. Our findings show compositional alterations of intestinal microbiota in adult patients with INS and correlations between significantly altered taxa and clinical parameters, which points out the direction for the development of new diagnostics and therapeutic approaches targeted intestinal microbiota.

Gut Microbiota Dysbiosis in Children with Relapsing Idiopathic Nephrotic Syndrome

2018 ◽  
Vol 47 (3) ◽  
pp. 164-170 ◽  
Author(s):  
Shoji Tsuji ◽  
Chikushi Suruda ◽  
Masaki Hashiyada ◽  
Takahisa Kimata ◽  
Sohsaku Yamanouchi ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Gut Microbiota ◽  
Butyric Acid ◽  
Idiopathic Nephrotic Syndrome ◽  
Flow Cytometric Analysis ◽  
Metagenomic Analysis ◽  
Healthy Children ◽  
Cd4 Cells ◽  
Acid Producing Bacteria ◽  
Gut Microbiota Dysbiosis

Background: While the etiology of idiopathic nephrotic syndrome (idiopathic nephrotic syndrome [INS]; characterized by repeated relapses and comorbid allergic conditions) remains unknown, recent evidence suggests that dysfunction in regulatory T cells (Tregs) plays an important role in the development of INS as well as allergic diseases. We hypothesized that dysbiosis involving decreased butyric acid-producing gut microbiota leads to defective induction and differentiation of peripherally induced Tregs, resulting in INS relapse. Methods: Study subjects were 12 children with INS, 8 classified as relapsing (R group; median age: 3.0 years) and 4 as non-relapsing (NR group; median age: 4.3 years), and 11 healthy children (HC group; median age: 5.1 years) serving as normal controls. Measurement of microbiota was performed using 16S ribosomal RNA metagenomic analysis, and fecal butyric acid was measured using high performance liquid chromatography. Flow-cytometric analysis of Tregs and CD4-positive (CD4+) cells in peripheral blood was also performed. Results: Metagenomic analysis of gut microbiota using feces showed that the proportion of butyric acid-producing bacteria was significantly lower in R (median 6.36%) than HC (median 18.84%; p = 0.0013), but no different between NR (median 16.71%) and HC (p = 0.29). Fecal organic acid analysis revealed significantly lower butyric acid quantities in R than HC (medians: 0.48 vs. 0.99 mg/g, p = 0.042). Circulating Tregs as a proportion of CD4+ cells were decreased in 75% of R and NR. Conclusion: Pediatric relapsing INS patients show gut microbiota dysbiosis, characterized by a decreased proportion of butyric acid-producing bacteria and lower fecal butyric acid quantities, concomitant with reduced circulatory Tregs.

scholarly journals P053 Overabundance of Lactobacillus species in gut microbiota of IBD patients

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S160-S161
Author(s):  
D Khusnutdinova ◽  
M Markelova ◽  
M Siniagina ◽  
E Boulygina ◽  
S Abdulkhakov ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Active Phase ◽  
Bioinformatic Analysis ◽  
Lactobacillus Species ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Shotgun Metagenomic Sequencing ◽  
Inflammatory Bowel ◽  
Stool Samples

Abstract Background Changes in the composition of gut microbiota, and their metabolic pathways, are important factors in the pathogenesis of inflammatory bowel disease (IBD). Many clinical trials have shown that taking probiotics based on Lactobacillus has a positive effect on patients with IBD. However, Lactobacillus should be used more carefully during the active phase of IBD, since some strains can negatively affect the pathogenesis of the disease1,2. The aim of this study was to assess the diversity of Lactobacillus species in the gut microbiome of IBD patients and healthy volunteers. Methods In the study, 62 stool samples from healthy people, 31 from patients with Crohn’s disease (CD), and 34 - ulcerative colitis (UC) in active phase were analyzed. DNA was isolated using the QIAamp Fast DNA Stool Mini Kit (Qiagen, USA) following with shotgun metagenomic sequencing the NextSeq 500 (project #0671-2020-0058). Bioinformatic analysis was performed with the MetaPhlAn2 package. Results An increased relative abundance of Lactobacillus was found in patients with IBD (3.2% ± 6.6% in CD and 1.6% ± 2.8 in UC) compared to healthy individuals (0.3% ± 1.2%, p<0.05). In the control group, Lactobacillus were absent in 41% of samples and 1–5 species were found in 58% of samples. Most CD and UC patients are characterized by the presence of 3 to 5 species of Lactobacillus (38% and 31%, respectively). For 23% of CD patients and 26% of UC patients, 6 to 9 types of Lactobacillus were found. Some patients with IBD have more than 10 different types of Lactobacillus in the gut microbiota (Fig.1). The intestinal microbiota in IBD patients is characterized by an increased abundance of several species: L. salivarius, L. gasseri, L. mucosae, as well as L. casei paracasei in patients with CD and L. vaginalis in patients with UC (Fig.2). Conclusion The composition of the intestinal microbiota of IBD patients differs significantly in terms of Lactobacillus proportion and species diversity. Overabundance of five Lactobacillus species could be associated with the active phase of IBD. References

scholarly journals Listeria monocytogenes faecal carriage is common and depends on the gut microbiota

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lukas Hafner ◽  
Maxime Pichon ◽  
Christophe Burucoa ◽  
Sophie H. A. Nusser ◽  
Alexandra Moura ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Sensu Stricto ◽  
Pathogenic Species ◽  
Faecal Carriage ◽  
Stool Samples ◽  
Systemic Infections ◽  
Prevalent Species ◽  
Human Stool ◽  
Independent Cohort

AbstractListeria genus comprises two pathogenic species, L. monocytogenes (Lm) and L. ivanovii, and non-pathogenic species. All can thrive as saprophytes, whereas only pathogenic species cause systemic infections. Identifying Listeria species’ respective biotopes is critical to understand the ecological contribution of Listeria virulence. In order to investigate the prevalence and abundance of Listeria species in various sources, we retrieved and analyzed 16S rRNA datasets from MG-RAST metagenomic database. 26% of datasets contain Listeria sensu stricto sequences, and Lm is the most prevalent species, most abundant in soil and host-associated environments, including 5% of human stools. Lm is also detected in 10% of human stool samples from an independent cohort of 900 healthy asymptomatic donors. A specific microbiota signature is associated with Lm faecal carriage, both in humans and experimentally inoculated mice, in which it precedes Lm faecal carriage. These results indicate that Lm faecal carriage is common and depends on the gut microbiota, and suggest that Lm faecal carriage is a crucial yet overlooked consequence of its virulence.

scholarly journals High-throughput cultivation of stable, diverse, fecal-derived microbial communities to model the intestinal microbiota

2020 ◽  
Author(s):  
Andrés Aranda-Díaz ◽  
Katharine Michelle Ng ◽  
Tani Thomsen ◽  
Imperio Real-Ramírez ◽  
Dylan Dahan ◽  
...  
Keyword(s):  
Animal Models ◽  
Gut Microbiota ◽  
Species Composition ◽  
Microbial Communities ◽  
Intestinal Microbiota ◽  
Initial Inoculum ◽  
Community Membership ◽  
Stool Samples

SummaryMechanistic understanding of the impacts of the gut microbiota on human health has been hampered by limited throughput in animal models. To enable systematic interrogation of gut-relevant microbial communities, here we generated hundreds of in vitro communities cultured from diverse stool samples in various media. Species composition revealed stool-derived communities that are phylogenetically complex, diverse, stable, and highly reproducible. Community membership depended on both medium and initial inoculum, with certain media preserving inoculum compositions. Different inocula yielded different community compositions, indicating their potential for personalized therapeutics. Communities were robust to freezing and large-volume culturing, enabling future translational applications. Defined communities were generated from isolates and reconstituted growth and composition similar to those of communities derived from stool inocula. Finally, in vitro experiments probing the response to ciprofloxacin successfully predicted many changes observed in vivo, including the resilience and sensitivity of each Bacteroides species. Thus, stool-derived in vitro communities constitute a powerful resource for microbiota research.

scholarly journals Compositional variation of the human fecal microbiome in relation to azo-reducing activity: a pilot study

Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Sara A. Zahran ◽  
Marwa Ali-Tammam ◽  
Amal E. Ali ◽  
Ramy K. Aziz
Keyword(s):  
Pilot Study ◽  
Gut Microbiota ◽  
Relative Abundance ◽  
Microbial Community Composition ◽  
Illumina Miseq ◽  
Compositional Variation ◽  
Fecal Microbiome ◽  
Azo Reduction ◽  
Stool Samples ◽  
Reducing Activity

Abstract Background Through an arsenal of microbial enzymes, the gut microbiota considerably contributes to human metabolic processes, affecting nutrients, drugs, and environmental poisons. Azoreductases are a predominant group of microbiota-derived enzymes involved in xenobiotic metabolism and drug activation, but little is known about how compositional changes in the gut microbiota correlate with its azo-reducing activity. Results To this end, we used high-throughput 16S rRNA amplicon sequencing, with Illumina MiSeq, to determine the microbial community composition of stool samples from 16 adults with different azo-reducing activity. High azo-reducing activity positively correlated with the relative abundance of phylum Firmicutes (especially genera Streptococcus and Coprococcus) but negatively with phylum Bacteroidetes (especially genus Bacteroides). Typical variations in the Firmicutes-to-Bacteroidetes and Prevotella-to-Bacteroides ratios were observed among samples. Multivariate analysis of the relative abundance of key microbial taxa and other diversity parameters confirmed the Firmicutes proportion as a major variable differentiating high and non-azo-reducers, while Bacteroidetes relative abundance was correlated with azo-reduction, sex, and BMI. Conclusions This pilot study showed that stool samples with higher azo-reducing activity were enriched in Firmicutes but with relatively fewer Bacteroidetes. More samples and studies from different geographical areas are needed to bolster this conclusion. Better characterization of different azoreductase-producing gut microbes will increase our knowledge about the fate and differential human responses to azodye-containing drugs or orally consumed chemicals, thus contributing to efforts towards implementing microbiome testing in precision medicine and toxicology.

Idiopathic nephrotic syndrome in children: role of regulatory T cells and gut microbiota

2020 ◽  
Author(s):  
Shoji Tsuji ◽  
Shohei Akagawa ◽  
Yuko Akagawa ◽  
Tadashi Yamaguchi ◽  
Jiro Kino ◽  
...  
Keyword(s):  
T Cells ◽  
Nephrotic Syndrome ◽  
Regulatory T Cells ◽  
Gut Microbiota ◽  
Idiopathic Nephrotic Syndrome

scholarly journals Causes of The Nephrotic Syndrome In Sweden 2014-2019 Depending On Clinical Presentation And Demographics

2021 ◽  
Author(s):  
Anneli Jönsson ◽  
Thomas Hellmark ◽  
Mårten Segelmark ◽  
Anna Forsberg ◽  
Karl Dreja
Keyword(s):  
Nephrotic Syndrome ◽  
Final Diagnosis ◽  
Underlying Disease ◽  
Response To Therapy ◽  
Adult Patients ◽  
Clinical Parameters ◽  
Plasma Albumin ◽  
Clinical Presentations ◽  
Grade 3 ◽  
Insufficient Knowledge

Abstract Background: Many different pathological processes can affect the integrity of the glomerular capillary wall and cause massive leakage of protein resulting in the Nephrotic Syndrome (NS). The prognosis and response to therapy differs depending on diagnosis, but renal biopsy cannot always be performed promptly. These is insufficient knowledge to which extent clinical parameters can predict the diagnosis. Methods: Age, gender, haematuria, proteinuria, plasma creatinine, plasma albumin and final diagnosis were retrieved for all adult patients with NS as indication for biopsy or massive albuminuria in conjunction with a low plasma albumin from the biopsy module of the Swedish Renal Registry (SRR) between 2014 and 2019. A basic calculator was developed to demonstrate the importance of clinical presentations in relation to the likelihood of having a specific diagnosis.Results: 913 unique patients were included in the study. Overall membranous nephropathy (17%) was the most common diagnoses, but when studying those <50 years old or women minimal change nephropathy (21 and 17 %) was the most frequent diagnosis. When examining those between 50 and 70 years-old, those with chronic kidney disease (CKD) 4 and those with negative dipstick tests for hematuria diabetic nephropathy (23, 30 and 21 %) was the most common underlying disease. Among those with high grade hematuria (grade 3-4 on dipsticks) Membranoproliferative glomerulonephritis was most common (14%), closely followed by IgA nephropathy (13%). Focal segmental glomerulosclerosis (9.7%) was less common than in many comparable studies. Conclusions: Clinical parameters have a profound impact on the likelihood of different diagnoses in adult patients with NS. Differences in clinical practice, inclusion criteria in studies and probably genetic background are important to account for when comparing data from different parts of the world.

Role of gut microbiota in idiopathic nephrotic syndrome in children

2017 ◽  
Vol 108 ◽  
pp. 35-37 ◽  
Author(s):  
Kazunari Kaneko ◽  
Shoji Tsuji ◽  
Takahisa Kimata
Keyword(s):  
Nephrotic Syndrome ◽  
Gut Microbiota ◽  
Idiopathic Nephrotic Syndrome

scholarly journals Clinical Significance of Probiotics for Children with Idiopathic Nephrotic Syndrome

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 365
Author(s):  
Tadashi Yamaguchi ◽  
Shoji Tsuji ◽  
Shohei Akagawa ◽  
Yuko Akagawa ◽  
Jiro Kino ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Treatment Group ◽  
Gut Microbiota ◽  
Idiopathic Nephrotic Syndrome ◽  
Oral Treatment ◽  
Immunosuppressive Agents ◽  
Treated Group ◽  
Probiotic Treatment ◽  
Before And After ◽  
To Receive

We previously reported that a decrease in butyrate-producing bacteria in the gut is a potential cause of regulatory T cell (Treg) abnormalities in children with idiopathic nephrotic syndrome (INS). Therefore, we hypothesized that administration of butyrate-producing bacteria might reduce INS relapse and the need for immunosuppressants in these patients. Twenty patients in remission from INS (median age 5.3 years, 15 boys) were enrolled in the study and assigned to receive either daily oral treatment with a preparation of 3 g Clostridium butyricum or no probiotic treatment. The number of relapses and requirement for immunosuppressive agents were compared between the two groups. In the probiotic treatment group, analyses of the gut microbiota and Treg measurements were also performed. Probiotic-treated patients experienced fewer INS relapses per year compared with non-probiotic-treated patients (p = 0.016). Further, administration of rituximab in the probiotic treatment group was significantly less frequent compared with the non-probiotic-treated group (p = 0.025). In the probiotic treatment group, analyses before and after probiotic treatment revealed the significant increases in the relative abundance of butyrate-producing bacteria (p = 0.017) and blood Treg counts (p = 0.0065). Thus, oral administration of butyrate-producing bacteria during INS remission may reduce the frequency of relapse and the need for immunosuppressive agents.

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