Current and Future Magnetic Resonance Technologies for Assessing Liver Disease in Clinical and Experimental Medicine

Background: In the past decades, a number of non-invasive methods have emerged for detecting and estimating liver fibrosis; these include both serum-based panels and imaging-based technology. Some of these methods are now being incorporated in clinical practice. However, the limitations of the current techniques include lack of organ specificity, sampling errors and limited ability to reflect the efficacy of interventions. Key Messages: Novel magnetic resonance (MR)-based techniques provide an opportunity to bring about further changes in the investigations and management of patients with liver diseases. Multimodal quantitative MR techniques enable the estimation of fat, iron accumulation, degree of liver injury/inflammation and fibrosis within the whole liver without the need for administering contrast agents. Architectural changes within the liver can be evaluated concurrently with portal haemodynamic changes allowing non-invasive assessment of portal hypertension and effects of interventions. A combination ultra-high field (7T) provides greater sensitivity with a potential to distinguish inflammation from fibrosis on imaging and determine specific types of fats (saturated vs. unsaturated) present within the liver using MR spectroscopy. 13C MR spectroscopy can estimate glutathione flux and rate of beta oxidation in-vivo providing novel tools for experimental studies that evaluate the efficacy of interventions as well as underlying mechanisms. Conclusions: Translational research should focus on converting the potentials of these innovative methodologies into clinical applications for the benefit of patients.

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In-Vivo Magnetic Resonance Spectroscopy III: In-Vivo MR Spectroscopy: Potential and Limitations

10.1007/978-3-642-77218-4 ◽

1992 ◽

Cited By ~ 2

Keyword(s):

Magnetic Resonance ◽

Magnetic Resonance Spectroscopy ◽

Mr Spectroscopy ◽

Resonance Spectroscopy

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Non-invasive diagnostics in fossils - Magnetic Resonance Imaging of pathological belemnites

Biogeosciences ◽

10.5194/bg-2-133-2005 ◽

2005 ◽

Vol 2 (2) ◽

pp. 133-140 ◽

Cited By ~ 18

Author(s):

D. Mietchen ◽

H. Keupp ◽

B. Manz ◽

F. Volke

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Soft Tissue ◽

Clinical Diagnostics ◽

Resonance Imaging ◽

Non Invasive ◽

Physiological Processes ◽

Magnetic Resonance Imaging Mri ◽

Invasive Diagnostics

Abstract. For more than a decade, Magnetic Resonance Imaging (MRI) has been routinely employed in clinical diagnostics because it allows non-invasive studies of anatomical structures and physiological processes in vivo and to differentiate between healthy and pathological states, particularly of soft tissue. Here, we demonstrate that MRI can likewise be applied to fossilized biological samples and help in elucidating paleopathological and paleoecological questions: Five anomalous guards of Jurassic and Cretaceous belemnites are presented along with putative paleopathological diagnoses directly derived from 3D MR images with microscopic resolution. Syn vivo deformities of both the mineralized internal rostrum and the surrounding former soft tissue can be traced back in part to traumatic events of predator-prey-interactions, and partly to parasitism. Besides, evidence is presented that the frequently observed anomalous apical collar might be indicative of an inflammatory disease. These findings highlight the potential of Magnetic Resonance techniques for further paleontological applications.

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BIMG-08. DEUTERIUM MAGNETIC RESONANCE SPECTROSCOPY USING 2H-PYRUVATE ALLOWS NON-INVASIVE IN VIVO IMAGING OF TERT EXPRESSION IN BRAIN TUMORS

Neuro-Oncology Advances ◽

10.1093/noajnl/vdab024.007 ◽

2021 ◽

Vol 3 (Supplement_1) ◽

pp. i2-i2

Author(s):

Georgios Batsios ◽

Celine Taglang ◽

Meryssa Tran ◽

Anne Marie Gillespie ◽

Joseph Costello ◽

...

Keyword(s):

Magnetic Resonance ◽

Magnetic Resonance Spectroscopy ◽

In Vivo Imaging ◽

Lactate Production ◽

Telomere Maintenance ◽

Resonance Spectroscopy ◽

Low Grade ◽

Non Invasive ◽

Tert Expression

Abstract Telomere shortening constitutes a natural barrier to uncontrolled proliferation and all tumors must find a mechanism of maintaining telomere length. Most human tumors, including high-grade primary glioblastomas (GBMs) and low-grade oligodendrogliomas (LGOGs) achieve telomere maintenance via reactivation of the expression of telomerase reverse transcriptase (TERT), which is silenced in normal somatic cells. TERT expression is, therefore, a driver of tumor proliferation and, due to this essential role, TERT is also a therapeutic target. However, non-invasive methods of imaging TERT are lacking. The goal of this study was to identify magnetic resonance spectroscopy (MRS)-detectable metabolic biomarkers of TERT expression that will enable non-invasive visualization of tumor burden in LGOGs and GBMs. First, we silenced TERT expression by RNA interference in patient-derived LGOG (SF10417, BT88) and GBM (GS2) models. Our results linked TERT silencing to significant reductions in steady-state levels of NADH in all models. NADH is essential for the conversion of pyruvate to lactate, suggesting that measuring pyruvate flux to lactate could be useful for imaging TERT status. Recently, deuterium (2H)-MRS has emerged as a novel, clinically translatable method of monitoring metabolic fluxes in vivo. However, to date, studies have solely examined 2H-glucose and the use of [U-2H]pyruvate for non-invasive 2H-MRS has not been tested. Following intravenous injection of a bolus of [U-2H]pyruvate, lactate production was higher in mice bearing orthotopic LGOG (BT88 and SF10417) and GBM (GS2) tumor xenografts relative to tumor-free mice, suggesting that [U-2H]pyruvate has the potential to monitor TERT expression in vivo. In summary, our study, for the first time, shows the feasibility and utility of [U-2H]pyruvate for in vivo imaging. Importantly, since 2H-MRS can be implemented on clinical scanners, our results provide a novel, non-invasive method of integrating information regarding a fundamental cancer hallmark, i.e. TERT, into glioma patient management.

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P1045 : Ultra-high-field MR-spectroscopy in NAFLD as non-invasive in-vivo tool for monitoring changes in fat and energy metabolism with potential identification of NASH and advanced fibrosis by saturation transfer technique

Journal of Hepatology ◽

10.1016/s0168-8278(15)31243-5 ◽

2015 ◽

Vol 62 ◽

pp. S739-S740 ◽

Cited By ~ 3

Author(s):

S. Traussnigg ◽

C. Kienbacher ◽

M. Gajdošík ◽

L. Valkovič ◽

H. Hofer ◽

...

Keyword(s):

Energy Metabolism ◽

Mr Spectroscopy ◽

High Field ◽

Advanced Fibrosis ◽

Saturation Transfer ◽

Ultra High Field ◽

Non Invasive ◽

High Field Mr

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Introduction to in vivo31P magnetic resonance spectroscopy of (human) skeletal muscle

Proceedings of The Nutrition Society ◽

10.1017/s0029665199001160 ◽

1999 ◽

Vol 58 (4) ◽

pp. 861-870 ◽

Cited By ~ 32

Author(s):

A. Heerschap ◽

C. Houtman ◽

H. J. A. in 't Zandt ◽

A. J. van den Bergh ◽

B. Wieringa

Keyword(s):

Skeletal Muscle ◽

Magnetic Resonance ◽

Magnetic Resonance Spectroscopy ◽

Current Status ◽

Resonance Spectroscopy ◽

Biochemical Processes ◽

Non Invasive ◽

Introductory Overview ◽

Muscle Biochemistry

31P magnetic resonance spectroscopy (MRS) offers a unique non-invasive window on energy metabolism in skeletal muscle, with possibilities for longitudinal studies and of obtaining important bioenergetic data continuously and with sufficient time resolution during muscle exercise. The present paper provides an introductory overview of the current status of in vivo31P MRS of skeletal muscle, focusing on human applications, but with some illustrative examples from studies on transgenic mice. Topics which are described in the present paper are the information content of the 31P magnetic resonance spectrum of skeletal muscle, some practical issues in the performance of this MRS methodology, related muscle biochemistry and the validity of interpreting results in terms of biochemical processes, the possibility of investigating reaction kinetics in vivo and some indications for fibre-type heterogeneity as seen in spectra obtained during exercise.

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The Anticancer Effects of Paeoniflorin and Its Underlying Mechanisms

Natural Product Communications ◽

10.1177/1934578x19876409 ◽

2019 ◽

Vol 14 (9) ◽

pp. 1934578X1987640

Author(s):

Li-Juan Deng ◽

Yu-He Lei ◽

Tsz-Fung Chiu ◽

Ming Qi ◽

Hua Gan ◽

...

Keyword(s):

Recent Progress ◽

Antitumor Effect ◽

Molecular Mechanisms ◽

Experimental Studies ◽

Future Prospects ◽

Anticancer Effects ◽

Wide Range ◽

Underlying Mechanisms

Paeoniflorin (PF) is an important pharmacological component of some Chinese traditional herbal formulas, such as Bai Shao, Chi Shao, and Dan Pi, which have been clinically used for centuries. Although many experimental studies have explored a wide range of pharmacological properties of PF, including anticancer, anti-inflammatory, antioxidant, immunoregulatory, and prevention of insulin resistance, there is no review to describe these reported effects systematically, especially the antitumor effect and the underlying mechanisms. In this review, we summarize the recent progress on the anticancer profiles both in vitro and in vivo of PF. Moreover, we highlight the integrated molecular mechanisms of PF and contemplate its future prospects as a potential anticancer drug.

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Non-Invasive Prediction of IDH Mutation in Patients with Glioma WHO II/III/IV Based on F-18-FET PET-Guided In Vivo 1H-Magnetic Resonance Spectroscopy and Machine Learning

Cancers ◽

10.3390/cancers12113406 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3406

Author(s):

Elisabeth Bumes ◽

Fro-Philip Wirtz ◽

Claudia Fellner ◽

Jirka Grosse ◽

Dirk Hellwig ◽

...

Keyword(s):

Machine Learning ◽

Magnetic Resonance ◽

Magnetic Resonance Spectroscopy ◽

Support Vector ◽

Resonance Spectroscopy ◽

Idh Mutation ◽

1H Mrs ◽

1H Magnetic Resonance Spectroscopy ◽

Non Invasive

Isocitrate dehydrogenase (IDH)-1 mutation is an important prognostic factor and a potential therapeutic target in glioma. Immunohistological and molecular diagnosis of IDH mutation status is invasive. To avoid tumor biopsy, dedicated spectroscopic techniques have been proposed to detect D-2-hydroxyglutarate (2-HG), the main metabolite of IDH, directly in vivo. However, these methods are technically challenging and not broadly available. Therefore, we explored the use of machine learning for the non-invasive, inexpensive and fast diagnosis of IDH status in standard 1H-magnetic resonance spectroscopy (1H-MRS). To this end, 30 of 34 consecutive patients with known or suspected glioma WHO grade II-IV were subjected to metabolic positron emission tomography (PET) imaging with O-(2-18F-fluoroethyl)-L-tyrosine (18F-FET) for optimized voxel placement in 1H-MRS. Routine 1H-magnetic resonance (1H-MR) spectra of tumor and contralateral healthy brain regions were acquired on a 3 Tesla magnetic resonance (3T-MR) scanner, prior to surgical tumor resection and molecular analysis of IDH status. Since 2-HG spectral signals were too overlapped for reliable discrimination of IDH mutated (IDHmut) and IDH wild-type (IDHwt) glioma, we used a nested cross-validation approach, whereby we trained a linear support vector machine (SVM) on the complete spectral information of the 1H-MRS data to predict IDH status. Using this approach, we predicted IDH status with an accuracy of 88.2%, a sensitivity of 95.5% (95% CI, 77.2–99.9%) and a specificity of 75.0% (95% CI, 42.9–94.5%), respectively. The area under the curve (AUC) amounted to 0.83. Subsequent ex vivo 1H-nuclear magnetic resonance (1H-NMR) measurements performed on metabolite extracts of resected tumor material (eight specimens) revealed myo-inositol (M-ins) and glycine (Gly) to be the major discriminators of IDH status. We conclude that our approach allows a reliable, non-invasive, fast and cost-effective prediction of IDH status in a standard clinical setting.

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Noninvasive Measurements of Human Brain Temperature Using Volume-Localized Proton Magnetic Resonance Spectroscopy

Journal of Cerebral Blood Flow & Metabolism ◽

10.1097/00004647-199704000-00001 ◽

1997 ◽

Vol 17 (4) ◽

pp. 363-369 ◽

Cited By ~ 71

Author(s):

Ron Corbett ◽

Abbot Laptook ◽

Paul Weatherall

Keyword(s):

Magnetic Resonance ◽

Frontal Lobe ◽

Mr Spectroscopy ◽

Brain Temperature ◽

Human Subjects ◽

Normal Subjects ◽

Whole Body ◽

Resonance Spectroscopy ◽

Noninvasive Measurements

Elucidation of the role of cerebral hyperthermia as a secondary factor that worsens outcome after brain injury, and the therapeutic application of modest brain hypothermia would benefit from noninvasive measurements of absolute brain temperature. The present study was performed to evaluate the feasibility of using 1H magnetic resonance (MR) spectroscopy to measure absolute brain temperature in human subjects on a clinical imaging spectroscopy system operating at a field strength of 1.5 T. In vivo calibration results were obtained from swine brain during whole-body heating and cooling, with concurrent measurements of brain temperature via implanted probes. Plots of the frequency differences between the in vivo MR peaks of water and N-acetyl-aspartate and related compounds (NAX), or water and choline and other trimethylamines versus brain temperature were linear over the temperature range studied (28–40°C). These relationships were used to estimate brain temperature from 1H MR spectra obtained from 10 adult human volunteers from 4 cm3-volumes selected from the frontal lobe and thalamus. Oral and forehead temperatures were monitored concurrently with MR data collection to verify normothermia in all the subjects studied. Temperatures determined using N-acetyl-aspartate or choline as the chemical shift reference did not differ significantly, and therefore results from these estimates were averaged. The brain temperature (mean ± SD) measured from the frontal lobe (37.2 = 0.6°C) and thalamus (37.7 ± 0.6°C) were significantly different from each other (paired t-test, p = 0.035). We conclude that 1H MR spectroscopy provides a viable noninvasive means of measuring regional brain temperatures in normal subjects and is a promising approach for measuring temperatures in brain-injured subjects.

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