scholarly journals Spectrin and Other Membrane-Skeletal Components in Human Red Blood Cells of Different Age

2017 ◽  
Vol 42 (3) ◽  
pp. 1139-1152 ◽  
Author(s):  
Annarita Ciana ◽  
Cesare Achilli ◽  
Giampaolo Minetti
Keyword(s):  
Red Blood Cells ◽  
Surface Area ◽  
Blood Cells ◽  
Ubiquitin Proteasome System ◽  
Membrane Skeleton ◽  
Experimental Conditions ◽  
Human Red Blood Cells ◽  
Cell Age

Background: Old human red blood cells (RBCs) have a reduced surface area with respect to young RBCs. If this decrease occurred through the release of vesicles similar to the spectrin-free vesicles that are shed in vitro under different experimental conditions or during storage, there would be no decrease of membrane-skeleton, but only of lipid bilayer surface area, during RBC ageing in vivo. However, we observed a decrease in spectrin and other membrane-skeletal proteins in old RBCs. Because RBCs contain components of the ubiquitin-proteasome system and other hydrolytic systems for protein degradation, we asked whether increased membrane-skeleton fragments could be detected in older RBCs. Methods: Four different anti-spectrin antibodies and an antibody anti-ubiquitin conjugates were used to analyse, by Western blotting, fragments of spectrin and other proteins in RBCs of different age separated in density gradients and characterized for their protein 4.1a/4.1b ratio as a cell age parameter. Results: spectrin fragments do exist in RBCs of all ages, they represent a minute fraction of all spectrin, are membrane-bound and not cytoplasmic and do not increase with cell age. Besides spectrin, other membrane-skeletal components decrease with cell age. Conclusion: Observed results challenge the commonly accepted view that decrease in cell membrane throughout RBC life in vivo occurs via the release of spectrin-free vesicles.

Decarboxylation of Radioactive DOPA by Erythrocytes in Schizophrenia

1971 ◽  
Vol 118 (545) ◽  
pp. 465-466 ◽  
Author(s):  
Ngo Tran ◽  
Marcel Laplante ◽  
Etienne Lebel
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Ionization Chamber ◽  
Present Experiment ◽  
Continuous Measurement ◽  
Human Tissues ◽  
Chamber Method ◽  
Human Red Blood Cells

The decarboxylation of 3, 4-dihydroxyphenyl-alanine (Dopa) to dopamine has been shown previously in animal and human tissues in both in vitro and in vivo studies (Sourkes, 1966; Vogel et al., 1970). However, very little information is available as to whether or not the decarboxylation of Dopa occurs in human red blood cells (RBC). In the present experiment we demonstrated this change in RBC from normals and from schizophrenics. An ionization chamber method was used for an instantaneous and continuous measurement of 14CO2 production from DL-dopa-carboxyl-14C by RBC in vitro.

scholarly journals 2,3-Diphosphoglycerate Content and Oxygen Affinity as a Function of Red Cell Age in Normal Individuals

Blood ◽  
1971 ◽  
Vol 38 (4) ◽  
pp. 463-467 ◽  
Author(s):  
STAVROS HAIDAS ◽  
DOMINIQUE LABIE ◽  
JEAN-CLAUDE KAPLAN
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Oxygen Affinity ◽  
Red Cell ◽  
In Vitro Storage ◽  
Cell Age ◽  
Normal Individuals ◽  
2,3 Dpg

Abstract A parallel decline of 2,3-diphosphoglycerate (2,3-DPG) and P50 of intracorpuscular hemoglobin is found in red blood cells during their in vivo aging. After 2,3-DPG depletion due to in vitro storage, the capacity to restore, 2,3-DPG in the presence of inosine is significantly impaired in senescent cells as compared with young cells.

In vivo and in vitro penetration of vitamins into human red blood cells

1971 ◽  
Vol 24 (8) ◽  
pp. 924-929 ◽  
Author(s):  
Michael F. Sorrell ◽  
Oscar Frank ◽  
Hermes Aquino ◽  
Allan D. Thomson ◽  
Herman Baker
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

scholarly journals Membrane Remodelling and Vesicle Formation During Ageing of Human Red Blood Cells

2017 ◽  
Vol 42 (3) ◽  
pp. 1127-1138 ◽  
Author(s):  
Annarita Ciana ◽  
Cesare Achilli ◽  
Anjali Gaur ◽  
Giampaolo Minetti
Keyword(s):  
Red Blood Cells ◽  
Lipid Bilayer ◽  
Blood Cells ◽  
Membrane Surface ◽  
High Surface ◽  
Volume Ratio ◽  
Membrane Skeleton ◽  
Protein Marker

Background/Aims: A high surface-to-volume ratio and a spectrin membrane-skeleton (MS) confer to the mammalian red blood cells (RBCs) their characteristic deformability, mechanical strength and structural stability. During their 120 days of circulatory life in humans, RBCs decrease in size, while remaining biconcave disks, owing to a coordinated decrease in membrane surface area and cell water. It is generally believed that part of the membrane is lost with the shedding of spectrin-free vesicles of the same type that can be obtained in vitro by different treatments. If this were true, an excess of MS would arise in old RBCs, with respect to the lipid bilayer. Aim of this paper was to investigate this aspect. Methods: Quantification of spectrin by electrophoretic methods was carried out in RBCs of different age. Results: Spectrin decreases, on a per cell basis, with RBC ageing. On the other hand, the membrane raft protein marker flotillin-2, while decreasing in the membrane of old cells, was found to be strongly depleted in the membrane of in vitro-induced vesicles. Conclusion: Part of the membrane-skeleton is probably lost together with part of the lipid bilayer in a balanced way. These findings point to a mechanism for the in vivo release of membrane that is different from that which is known to occur in vitro.

Alteration of human red blood cells stored in plastic packs. A case report with in vivo and in vitro studies

Transfusion ◽  
1982 ◽  
Vol 22 (2) ◽  
pp. 154-157 ◽  
Author(s):  
JT Callahan ◽  
MF Collecutt ◽  
JR Lightbody ◽  
BS Faragher
Keyword(s):  
Case Report ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Vitro Studies ◽  
Human Red Blood Cells

Lysis of Human Red Blood Cells. 4. Comparison of in Vitro and in Vivo Hemolysis Data

1997 ◽  
Vol 86 (11) ◽  
pp. 1215-1217 ◽  
Author(s):  
Joseph F. Krzyzaniak ◽  
Fernando A. Alvarez Núñez ◽  
Dawn M. Raymond ◽  
Samuel H. Yalkowsky
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Red Blood Cells

scholarly journals Identification of Oxidative Stress in Red Blood Cells with Nanoscale Chemical Resolution by Infrared Nanospectroscopy

2018 ◽  
Vol 19 (9) ◽  
pp. 2582 ◽  
Author(s):  
Francesco Ruggeri ◽  
Curtis Marcott ◽  
Simone Dinarelli ◽  
Giovanni Longo ◽  
Marco Girasole ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Membrane Skeleton ◽  
Force Microscopy ◽  
Atomic Force ◽  
Membrane Peroxidation

During their lifespan, Red blood cells (RBC), due to their inability to self-replicate, undergo an ageing degradation phenomenon. This pathway, both in vitro and in vivo, consists of a series of chemical and morphological modifications, which include deviation from the biconcave cellular shape, oxidative stress, membrane peroxidation, lipid content decrease and uncoupling of the membrane-skeleton from the lipid bilayer. Here, we use the capabilities of atomic force microscopy based infrared nanospectroscopy (AFM-IR) to study and correlate, with nanoscale resolution, the morphological and chemical modifications that occur during the natural degradation of RBCs at the subcellular level. By using the tip of an AFM to detect the photothermal expansion of RBCs, it is possible to obtain nearly two orders of magnitude higher spatial resolution IR spectra, and absorbance images than can be obtained on diffraction-limited commercial Fourier-transform Infrared (FT-IR) microscopes. Using this approach, we demonstrate that we can identify localized sites of oxidative stress and membrane peroxidation on individual RBC, before the occurrence of neat morphological changes in the cellular shape.

In vitro and in vivo Binding of Epirubicin to Red Blood Cells and Human Plasma Proteins

1994 ◽  
Vol 49 (7-8) ◽  
pp. 483-488 ◽  
Author(s):  
Suzan Bandak ◽  
Martin Czejka ◽  
Johann Schüller
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Proteins ◽  
In Vivo Binding ◽  
Human Red Blood Cells ◽  
Encapsulation Rate ◽  
Vitro Binding ◽  
In Vitro Interaction

In this study, the in vitro interaction of epirubicin (EPR), a cytostatic antibiotic, with plasma proteins (PP), namely α-HSA, γ-HSG, α+β-HSG and with isolated human red blood cells (RBCs) was investigated and further correlated with the in vivo pharmacokinetics and binding of EPR and two of its metabolites, 13-dihydroepirubicin and 7-deoxydoxorubicinone to RBCs. The in vitro encapsulation rate in isolated erythrocytes amounts to 52.9 ± 2.8% and remains constant within the range of studied concentrations (2.5-20 μg/ml). EPR was found to bind differently to the various PP in vitro. Binding to α-HSA amounted up to 51.0 ± 7.10%, to α + β-HSG 79.45 ± 2.7%, to γ-HSG 57.1 ± 2.8%. The in vivo-binding rate of EPR, dihydroepirubicin and deoxydoxorubicinone to RBCs after 5 min of injection was 32 ± 6.96%, 11.6 ± 3.1% and 10.05 ± 3.5% respectively, their availability in serum was 42.6 ± 11.8%, 2.4 ± 0.4% and 1.2 ± 0.67% respectively

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

scholarly journals Multimodal Diagnostics of Microrheologic Alterations in Blood of Coronary Heart Disease and Diabetic Patients

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 76
Author(s):  
Anastasia Maslianitsyna ◽  
Petr Ermolinskiy ◽  
Andrei Lugovtsov ◽  
Alexandra Pigurenko ◽  
Maria Sasonko ◽  
...  
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Optical Methods ◽  
Diabetic Patients ◽  
Aggregation Index ◽  
Significant Difference

Coronary heart disease (CHD) has serious implications for human health and needs to be diagnosed as early as possible. In this article in vivo and in vitro optical methods are used to study blood properties related to the aggregation of red blood cells in patients with CHD and comorbidities such as type 2 diabetes mellitus (T2DM). The results show not only a significant difference of the aggregation in patients compared to healthy people, but also a correspondence between in vivo and in vitro parameters. Red blood cells aggregate in CHD patients faster and more numerously; in particular the aggregation index increases by 20 ± 7%. The presence of T2DM also significantly elevates aggregation in CHD patients. This work demonstrates multimodal diagnostics and monitoring of patients with socially significant pathologies.

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