scholarly journals Comparison of a New IgG-EIA for the Detection of Anti-Plasmodium Antibodies with Two Currently Used Assays

2021 ◽  
pp. 1-7
Author(s):  
Christoph Niederhauser ◽  
Caroline Tinguely ◽  
Jens Dreier ◽  
Tanja Vollmer ◽  
Hans Peter Marti ◽  
...  
Keyword(s):  
Blood Donation ◽  
Index Test ◽  
Protozoan Parasites ◽  
Assay Sensitivity ◽  
Blood Samples ◽  
Reference Centre ◽  
Blood Donor Screening ◽  
Blood Banks ◽  
Diagnostic Centre ◽  
Public Health Institute

<b><i>Background:</i></b> Malaria is a mosquito-borne infectious disease caused by protozoan parasites of the genus <i>Plasmodium.</i> As migration of populations from endemic areas to Europe and overseas recreational travel to endemic regions increase, there is also a growing risk of transfusion-transmitted tropical diseases by blood components. <b><i>Material and Methods:</i></b> In the present study two routine <i>Plasmodium</i> spp. ELISA (CAPTIA™ Malaria EIA, Trinity Biotech, and Malaria EIA, BioRad) were compared with a new commercial ELISA (ELISA IgG, EUROIMMUN). From December 1, 2015 until November 30, 2016, 1,096 plasma samples from blood donors with a potential risk of malaria infection were collected at two blood transfusion centres in Germany and Switzerland. <b><i>Results:</i></b> The samples were tested comparatively with the ELISA from EUROIMMUN and the routine test used at the respective centre. Thirty-four of 595 (5.7%) tested blood samples from centre 1 and 49 of 501 (9.8%) tested blood samples from centre 2 showed reactivity on either or both ELISAs. All 83 reactive samples were sent for confirmation to the Diagnostic Centre of the Swiss Tropical and Public Health Institute (Swiss TPH) in Basel, Switzerland. Sixteen samples, which previously were reactive in the routine <i>Plasmodium</i> spp. EIA assays, were proven positive after confirmation testing (i.e., 4 positive and 12 inconclusive results), indicating an anti-<i>Plasmodium</i> antibody prevalence in blood donations of 1.5%. From these 16 reactive samples, 13 were also detected by the index test, resulting in an assay sensitivity of 81.2%. A specificity of 98.6% was calculated (1,065/1,080 confirmed negative samples). The overall agreement with the reference centre was 95.8% in centre 1 and 94% in centre 2. <b><i>Conclusion:</i></b> The comparison of the new EUROIMMUN ELISA and the established CAPTIA™ Malaria EIA (Trinity Biotech) and Malaria EIA (BioRad) used for routine blood donor screening in two laboratory blood donation centres revealed that all tested ELISAs show comparable sensitivities and are equally suitable for anti-<i>Plasmodium</i> antibody screening in blood banks.

Analysing repeat blood donation behavior via big data

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Xu Chen ◽  
Lei Liu ◽  
Xinyu Guo
Keyword(s):  
Junior High School ◽  
Blood Donors ◽  
Blood Donation ◽  
Average Frequency ◽  
Average Volume ◽  
Content Type ◽  
Blood Banks ◽  
Supply Chain Management System ◽  
Donation Behavior ◽  
The Impact

PurposeAlthough blood banks have recently started to recruit blood donors through social media platforms, including WeChat, to increase recruitment effectiveness, few researchers have studied their effects on blood donation behavior. The aim of this study is to examine the influence of using official WeChat accounts on repeat blood donation behavior.Design/methodology/approachThis paper used the backstage operation data of official WeChat accounts and blood supply chain management system data from the blood bank for the study to analyze the changes in repeat blood donation behavior. First, to analyze the changes in the average frequency of blood donation per year, average volume of single blood donation and blood eligible rate of repeat blood donors before and after following the official WeChat accounts by difference-in-differences model combined with propensity score matching (PSM-DID). Second, we examined the impact of official WeChat accounts on the proportion of repeat blood donors through survival analysis.FindingsThe results show that following WeChat accounts increases the average frequency of blood donation and blood eligible rate of repeat blood donors by 14.36% and 1.19%, respectively, and have no significant effect on the average volume of single blood donation. Further, WeChat accounts have a more significant impact on the average frequency of blood donations per year for workers, farmers, medical staff and groups with education levels of junior high school. In addition, official WeChat accounts can effectively increase the proportion of repeat donors.Originality/valueThe results provide a quantitative basis for the influence of official WeChat accounts on repeat blood donation behaviors. On the one hand, it is of great significance to guide the publicity and recruitment of unpaid blood banks. On the other hand, it provides an evidence for the promotion of official WeChat accounts.

scholarly journals VECTOR-BORNE PATHOGENS-TESTING IN A ROMANIAN CANINE BLOOD BANK

2018 ◽  
Vol 6 ◽  
pp. 904-911
Author(s):  
Teodor-Stefan Ionescu ◽  
Sinziana Radulescu ◽  
Ruxandra Florea ◽  
Stelian Baraitareanu ◽  
Doina Danes
Keyword(s):  
Blood Donors ◽  
Holistic Approach ◽  
Blood Bank ◽  
Focused Attention ◽  
Donor Pool ◽  
Blood Samples ◽  
Negative Results ◽  
Blood Banks ◽  
Vector Borne ◽  
Positive Results

INTRODUCTION: Canine blood banking in veterinary medicine is an expanding market. Once the demand for blood products increased all over the world, canine blood banks have focused attention on the risk of spreading diseases through blood transfused products. The need to preserve a healthy donor-pool, free of blood-borne infectious diseases, mainly in endemic areas, led to the implementation of appropriate protocols for screening canine blood donors using specific tests.OBJECTIVES: The aim of this study was to evaluate the presence of Anaplasma phagocytophilum/Anaplasmaplatys, Echrlichiacanis/Echrlichiaewingii, Dirofilariaimmitis and Borrelia burgdorferi using the enzyme immunoassay technology (EIA) among the donors of a Romanian canine blood bank, from January 2015 to December 2016.METHODS: Blood samples from 575 donors were collected and 1253 tests were performed with SNAP 4DX Plus® (IDEXX Laboratories, Fremont, CA) to reveal the presence of D. immitis antigens and the antibodies toward A. phagocytophilum and/or A. platys, E. canis and/or E. ewingii and B. burgdorferi.RESULTS: The results of this holistic approach show that all blood samples provided negative results for B. burgdorferi and E.canis/E. ewingii (0/1253), while 0.87% (11/1253) samples provided positive results for A. phagocytophilum/A. platys and 6.94% (87/1253) for D. immitis.CONCLUSION: The next studied topic would be to compare the results provided by the EIA technology with results of real time PCR and qPCR regarding these vector-borne pathogens.

scholarly journals Seroprevalence of markers of transfusiontransmissible diseases in positive blood donors detected in routine screening in the Blood Bank of Hospital Carlos Luis Valverde Vega, Costa Rica. Period 2015 – 2019

2021 ◽  
Vol 9 (4) ◽  
pp. 70-73
Author(s):  
Jean Pablo Castro-Ruiz
Keyword(s):  
Infectious Diseases ◽  
Blood Donors ◽  
Blood Donation ◽  
Individual Characteristics ◽  
Blood Bank ◽  
Health Centers ◽  
Blood Components ◽  
Blood Banks ◽  
Scientific Ethics ◽  
The Individual

Blood is one of the fundamental tissues of our body. Blood donation allows blood banks to meet hospital needs for blood components. Blood transfusion is an activity that carries a risk, originated by a series of causal actions derived from the individual characteristics of donors and patients. Blood components are biological products and as such, they can never be exempt from the risk of transmitting infectious diseases, such as hepatitis B, HIV, syphilis, among others. Thanks to the investigation of the donor database of the Blood Bank of Hospital Dr. Carlos Luis Valverde Vega and the annual statistics of the blood bank, the statistics referring to donations were obtained, according to the type, grade of acceptance and as well as the prevalence of mandatory screening infectious diseases in the blood bank, between the years 2015 and 2019, with the respective authorization of the corresponding scientific ethics committee. This study reported a prevalence for Anti HB Core of 0.99%, 0.14% for HBsAg, 0.11% for syphilis, 0.05% for hepatitis C, 0.02% for HIV and 0% for the disease de Chagas and HTLV I / II. By using the bases of this work, future analyzes with a similar premise may be developed in different blood banks of the different health centers in our country.

TESty – Typing Easier: an innovate device to characterize blood samples

2020 ◽  
Vol 30 (Supplement_2) ◽  
Author(s):  
C Mourato ◽  
D Martins ◽  
R Teixo ◽  
F Mendes
Keyword(s):  
Healthcare Providers ◽  
Cost Effective ◽  
The Other ◽  
Blood Type ◽  
Agarose Gel ◽  
Laboratory Equipment ◽  
Blood Samples ◽  
Blood Banks ◽  
Support Device ◽  
Effective Product

Abstract Introduction In Portugal there are over 130,000 regular donors (2 to 4 blood donations/year), data from 2017. These data lead us to approximately 500,000 donations per year just in Portugal. In addition, we have over 300,000 transfusions per year that may require more than one unit of blood. All of these units, prior to administration, and all patients before a blood transfusion or even a transplant, must be properly classified regarding their blood type. Currently, the standard (tube) technique requires that at least 8 tubes be used per individual and the card, a device widely used today, requires the use of 1,5 per individual. It also requires the purchase of expensive laboratory equipment specifically for this purpose. The complex protocol, requiring access to laboratories with specific equipment without any other use (eg card centrifuges) and need to be performed by professionals specialized healthcare providers are the main disadvantages. Objectives Our main goal was to develop a new cost effective and efficient device- TESTy which could allow the evaluation of a higher number of samples discarding the need of any supplementary device or equipment. Methodology A 96-well V-bottom microplate was used as support device. After, an agarose gel and the antibodies serums were soaked in each well according to the specifications of the blood determination test. Results After a six month evaluation, TESty provide the same results as expected and previously tested with the other two techniques available at the market. Conclusion TESty can be revolutionary in the science transfusion world once it allows the screening of 12 samples simultaneously and this fact can save time and resources daily in our laboratories and blood banks. Also, for being a cost-effective product, it can be extended to undeveloped countries and contribute to assure the transfusion safety required.

scholarly journals Trends of T. cruzi infection based on data from blood bank screening

1990 ◽  
Vol 32 (2) ◽  
pp. 132-137 ◽  
Author(s):  
Fabio Zicker ◽  
Celina Maria Turchi Martelli ◽  
Ana Lucia Sampaio Sgambatti de Andrade ◽  
Simonne Almeida e Silva
Keyword(s):  
Rural Areas ◽  
Blood Donors ◽  
Blood Donation ◽  
Adult Population ◽  
Seroprevalence Rate ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Blood Banks ◽  
First Time ◽  
Cruzi Infection

Between October 1988 and April 1989 a cross-sectional survey was carried out in six out of eight blood banks of Goiânia, Central Brazil. Subjects attending for first-time blood donation in the mornings of the study period (n = 1358) were interviewed and screened for T. cruzi infection as a part of a major study among blood donors. Tests to anti-T. cruzi antibodies were performed, simultaneously, by indirect hem agglutination test (IHA) and complement fixation test (CFT). A subject was considered seropositive when any one of the two tests showed a positive result. Information on age, sex, place of birth, migration and socio-economic level was recorded. Results from this survey were compared with seroprevalence rates obtained in previous studies in an attempt to analyse trend of T. cruzi infection in an endemic urban area. The overall seroprevalence of T. cruzi infection among first-time donors was found to be 3.5% (95% confidence interval 2.5%-4.5% ). The seroprevalence rate increased with age up to 45 years and then decreased. Migrants from rural areas had higher seroprevalence rates than subjects from urban counties (1.8%-16.2% vs. 0%-3.6%). A four fold decrease in prevalence rates was observed when these rates were compared with those of fifteen years ago. Two possible hypotheses to explain this difference were suggested: 1. a cohort effect related with the decrease of transmission in rural areas and/or 2. a differential proportion of people of rural origin among blood donors between the two periods. The potential usefulness of blood banks as a source of epidemiological information to monitor trends of T. cruzi infection in an urban adult population was stressed.

scholarly journals Hepatitis B and Malaria Among Nepalese Blood Donors

1970 ◽  
Vol 5 (5) ◽  
pp. 81-84 ◽  
Author(s):  
Prakash Ghimire ◽  
Bishnu Bhakta Dhungyel ◽  
Bishnu Raj Tiwari
Keyword(s):  
Hepatitis B ◽  
Viral Hepatitis ◽  
Surface Antigen ◽  
Disease Burden ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Malarial Parasite ◽  
Blood Samples ◽  
Blood Banks ◽  
Donated Blood

Viral hepatitis and malaria, both are the diseases with noticeable disease burden in Nepal. Malaria is seasonal with high disease burden during post rainy season. Severe malaria also shows similar symptoms to viral hepatitis. This is collaborative study has been conducted during June - September 2006 with the objective of determining the prevalence of hepatitis B and malaria in Nepalese blood donors to find out the need of routine malaria testing in each pint of donated blood. During the study period, screening of malaria and hepatitis B surface antigen (HBsAg) were done in 1200 blood samples collected from blood donors at Kathmandu, Nepalgunj and Biratnagar Blood Banks. Malaria diagnosis was done using one drop of blood from each unit of blood using Giemsa stained thick and thin smear microscopy, while hepatitis B surface antigen (HBsAg) was detected using commercial ELISA. Of the total 1200 blood samples analyzed, 600 were collected at Kathmandu and 300 each at Nepalgunj and Biratnagar blood banks, situated in malaria endemic southern districts. Among the total 1200 samples, 1% (12) of the sample was found reactive for HBsAg; while only 0.33 % (4) samples were positive for malarial parasite. 1.33% (8) samples from Kathmandu and 1.33% (4) samples from Nepalgunj were positive for HBV. 1% (3) samples from Nepalgunj and 0.33% (1) sample from Biratnagar were found to be positive for malarial parasite. None of the samples from Biratnagar were positive for HBsAg, while none of the samples from Kathmandu were positive for malarial parasite. All of the malaria cases were due to Plasmodium vivax. All the infected cases were male. Co-infection of malaria and HBV was not observed during the entire period of study. However more extensive study is needed in other endemic areas of Nepal, the study indicated that the continuation of screening each point of donated blood for Hepatitis B, while screening of malarial parasites in donors of endemic terai districts may be useful in preventing transmission of malaria through transfusion. Key words: Hepatitis; Malaria; ELISA; Blood donors; Blood transfusion. DOI: 10.3126/sw.v5i5.2661 Scientific World, Vol. 5, No. 5, July 2007 81-84

scholarly journals Hemochromatosis Patients as Voluntary Blood Donors

2004 ◽  
Vol 18 (6) ◽  
pp. 393-396 ◽  
Author(s):  
Tara E Power ◽  
Paul C Adams
Keyword(s):  
Quality Of Life ◽  
Physical Functioning ◽  
Blood Donors ◽  
Blood Donation ◽  
Bodily Pain ◽  
Medical Factors ◽  
Blood Banks ◽  
Potential Resource ◽  
Donated Blood

The present study was designed to investigate hemochromatosis patients' suitability as blood donors as well as their perceptions and experience with the current public donation system. Participants were gathered from a list of current hemochromatosis patients (n=120) and members of the Canadian Hemochromatosis Society (n=1000). Of the 1120 surveys mailed out to these groups, 801 surveys were returned completed. The sample respondents had a mean age of 57.44 years (SD=12.73; range 19 to 87 years), and 57% were men. It was found that 20% (160) of the respondents have donated blood since their diagnosis; however, only 12% of the respondents indicated that they use voluntary blood donation as a means of maintaining their iron levels. Forty per cent of the respondents indicated that they had been refused from voluntary donation. Despite the fact that in May 2001 the Canadian Blood Services, in collaboration with the Canadian Hemochromatosis Society, began a promotion campaign to encourage hemochromatosis patients to become voluntary blood donors, the present study found that 15% of the respondents reported having been refused from the voluntary blood donation service due to the diagnosis of hemochromatosis. With respect to quality of life, it was found that individuals who donate blood were generally healthier with respect to physical functioning and bodily pain, however, these findings may indicate that hemochromatosis patients who are healthier are better able to donate at public blood banks, rather than that voluntary blood donation has an effect on the donors' physical functioning over phlebotomy clinic users. These study findings suggest that although there may be other medical factors limiting individuals from donating, hemochromatosis patients are interested in being voluntary blood donors and this potential resource is currently under-used.

scholarly journals A study of the noncompliance of blood banks on safety and quality parameters in blood donation camps in Bengaluru

2015 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
RajatKumar Agarwal ◽  
Rakesh Dhanya ◽  
LalithG Parmar ◽  
Arpit Vaish ◽  
Amit Sedai ◽  
...  
Keyword(s):  
Blood Donation ◽  
Quality Parameters ◽  
Blood Banks

scholarly journals Prevalence of dog erythrocyte antigens 1, 4, and 7 in galgos (Spanish Greyhounds)

2015 ◽  
Vol 27 (4) ◽  
pp. 558-561 ◽  
Author(s):  
Eva Spada ◽  
Daniela Proverbio ◽  
Luis Miguel Viñals Flórez ◽  
Maria del Rosario Perlado Chamizo ◽  
Roberta Perego ◽  
...  
Keyword(s):  
Blood Donors ◽  
Blood Donation ◽  
Jugular Vein ◽  
Physical Characteristics ◽  
Blood Samples ◽  
Blood Types ◽  
Cell Counts ◽  
Erythrocyte Antigen ◽  
Relative Prevalence ◽  
Dog Erythrocyte

Galgos (Spanish Greyhounds), in common with other sighthounds, have higher hematocrits, hemoglobin concentrations, and red blood cell counts than other breeds. In addition to these hematological characteristics, the physical characteristics of these dogs (medium to large dogs with an easily accessible jugular vein and a good temperament) make galgos ideal blood donors. However, to date, there are only published reports concerning dog erythrocyte antigen (DEA) 1 in this breed. Information on DEAs 4 and 7 would be useful when recruiting blood donors to donation programs, as DEA 1 and 7–negative and DEA 4–positive dogs can be considered universal donors. Ethylenediamine tetra-acetic acid–anticoagulated jugular blood samples were collected from 205 galgos. Dogs were aged between 1 and 10 years, 102 were female (49.8%) and 103 male (50.2%), and all were living in South Madrid, Spain. All 205 blood samples were tested for DEA 1 by card agglutination, and 150 of these samples were tested for DEA 4 and DEA 7 by gel column agglutination using polyclonal anti-DEA antibodies. Of the 205 galgos blood samples typed, 112 out of 205 (54.6%) were positive for DEA 1. Of the 150 blood samples tested, all (150/150, 100%) were positive for DEA 4, and 12 out of 150 (8%) samples tested positive for DEA 7. Of these samples, 70 out of 150 (46.7%) were positive only for DEA 4. There was no relationship between blood types and sex. In addition to the hematological characteristics previously reported and the physical characteristics of these dogs, the relative prevalence of blood types DEA 1, 4, and 7 make galgos good candidates for blood donation in blood donor programs.

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