scholarly journals Investigation on the printability of bioink based on alginate-gelatin hydrogel and liquid crystals

2020 ◽  
Vol 9 (4) ◽  
pp. 1718-1725
Author(s):  
Alyaa Idrees Abdulmaged ◽  
Chin Fhong Soon ◽  
Balkis A. Talip ◽  
Sheril Amira Othman ◽  
Gim Pao Lim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liquid Crystals ◽  
Flow Rate ◽  
Potential Application ◽  
Cell Attachment ◽  
3D Bioprinting ◽  
Minimum Width ◽  
Gelatin Hydrogel ◽  
Support Cell ◽  
Gelatin Concentration

Bioinks of 3D bioprinting have significant potential application in the field of tissue engineering to support cell attachment and proliferation. In this work, the alginate-gelatin-CELC (AGLC) bioink based on different compositions of alginate-gelatin (AG) hydrogel and cholesteryl ester liquid crystals (CELC) was prepared. Primarily, the alginate-gelatin hydrogel with certain concentration of Gelatin (10-50%w/v) was investigated. The printability of the hydrogel reached a minimum width of 1.8 mm at a flow rate of 1 mL/min when the Gelatin concentration was increased to 50 % w/v (AG1050). Subsequently, the respective polymers with 10% w/v Alginate and50% w/v Gelatin blended with 1%, 5%, 10%, 20%, 40%, and 60% w/v of CELC in the preparation of the alginate-gelatin-CELC bioink was further investigated. The printability of the bioink was examined by micro-extrusion based 3D bioprinter. The printability of the bioink enhanced by 27.8% as compared to AG1050 and reached a minimum width of 1.3 mm at a flow rate of 1 mL/min when the CELC concentration was increased to 40% and 60%. The tested properties of the bioink show that the CELC improve shear-thinning and lipid moieties properties to the composite bioink and hence, enhances its printability.

Poly(L-Lactic Acid-Co-Aspartic Acid): Interactive Polymers for Tissue Engineering

1995 ◽  
Vol 394 ◽  
Author(s):  
Jeffrey S. Hrkach ◽  
Jean Ou ◽  
Noah Lotan ◽  
Robert Langer
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Cell Growth ◽  
Aspartic Acid ◽  
Biodegradable Polymers ◽  
Cell Attachment ◽  
Biologically Active ◽  
Functional Sites ◽  
Support Cell ◽  
Enhance Cell Attachment

AbstractOne of the challenges in the field of tissue engineering is the development of optimal materials for use as scaffolds to support cell growth and tissue development. For this purpose, we are developing synthetic, biodegradable polymers with functional sites that provide the opportunity to covalently attach biologically active molecules to the polymers, so they can predictably interact with cells in a favorable manner to enhance cell attachment and growth. The preparation of poly(L-lactic acid-co-aspartic acid) comb-like graft copolymers from poly(L-lactic acid-co-β-benzyl-L-aspartate), and the casting of polymer films by solvent evaporation were carried out.

scholarly journals Development of Gelatin-Coated Microspheres for Novel Bioink Design

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3339
Author(s):  
Muskan Kanungo ◽  
Yale Wang ◽  
Noah Hutchinson ◽  
Emma Kroll ◽  
Anna DeBruine ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Flow Rate ◽  
Bioactive Molecules ◽  
Great Promise ◽  
Charged Surface ◽  
Design Expert ◽  
Gelatin Concentration ◽  
Gelatin Coating ◽  
Positively Charged

A major challenge in tissue engineering is the formation of vasculature in tissue and organs. Recent studies have shown that positively charged microspheres promote vascularization, while also supporting the controlled release of bioactive molecules. This study investigated the development of gelatin-coated pectin microspheres for incorporation into a novel bioink. Electrospray was used to produce the microspheres. The process was optimized using Design-Expert® software. Microspheres underwent gelatin coating and EDC catalysis modifications. The results showed that the concentration of pectin solution impacted roundness and uniformity primarily, while flow rate affected size most significantly. The optimal gelatin concentration for microsphere coating was determined to be 0.75%, and gelatin coating led to a positively charged surface. When incorporated into bioink, the microspheres did not significantly alter viscosity, and they distributed evenly in bioink. These microspheres show great promise for incorporation into bioink for tissue engineering applications.

scholarly journals Characterization and Application of Carboxymethyl Chitosan-Based Bioink in Cartilage Tissue Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Yunfan He ◽  
Soroosh Derakhshanfar ◽  
Wen Zhong ◽  
Bingyun Li ◽  
Feng Lu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mechanical Performance ◽  
Ethylenediaminetetraacetic Acid ◽  
Cell Attachment ◽  
Cartilage Tissue Engineering ◽  
Mechanical Analysis ◽  
Cartilage Tissue ◽  
3D Bioprinting ◽  
Modified Chitosan

Chitosan is a promising natural biomaterial for biological application; however, the weak mechanical performance of pristine chitosan limits its further utilization in hard tissue (such as cartilage) engineering. In this study, a chitosan-based 3D printing bioink with suitable mechanical properties was developed as 3D bioprinting ink for chondrocyte support. Chitosan was first modified by ethylenediaminetetraacetic acid (EDTA) to provide more carboxyl groups followed by physical crosslinking with calcium to increase the hydrogel strength. Dynamic mechanical analysis was carried out to evaluate viscoelastic properties with the addition of modified chitosan. A bioink with a combination of modified and pristine chitosan was formulated for scaffold fabrication via 3D bioprinting technique. Furthermore, cell viability, cell proliferation, and expression of chondrogenic markers were evaluated in vitro in chondrocytes loaded on the bioink. The novel bioink exhibited a favorable mechanical property and promoted cell attachment and chondrogenic gene expression in chondrocytes. Based on these results, we can conclude that the presented bioink could qualify for use in 3D bioprinting in cartilage tissue engineering.

scholarly journals Motility Improvement of Biomimetic Trachea Scaffold via Hybrid 3D-Bioprinting Technology

Polymers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 971
Author(s):  
Young Soo Yu ◽  
Chi Bum Ahn ◽  
Kuk Hui Son ◽  
Jin Woo Lee
Keyword(s):  
Rotation Angle ◽  
Thick Layer ◽  
Cartilage Tissue ◽  
Soft Tissue Reconstruction ◽  
3D Bioprinting ◽  
Rotating Beam ◽  
Rotational Angle ◽  
Gelatin Hydrogel ◽  
Tissue Reconstruction ◽  
Neck Rotation

A trachea has a structure capable of responding to various movements such as rotation of the neck and relaxation/contraction of the conduit due to the mucous membrane and cartilage tissue. However, current reported tubular implanting structures are difficult to impelement as replacements for original trachea movements. Therefore, in this study, we developed a new trachea implant with similar anatomical structure and mechanical properties to native tissue using 3D printing technology and evaluated its performance. A 250 µm-thick layer composed of polycaprolactone (PCL) nanofibers was fabricated on a rotating beam using electrospinning technology, and a scaffold with C-shaped cartilage grooves that mimics the human airway structure was printed to enable reconstruction of cartilage outside the airway. A cartilage type scaffold had a highest rotational angle (254°) among them and it showed up to 2.8 times compared to human average neck rotation angle. The cartilage type showed a maximum elongation of 8 times higher than that of the bellows type and it showed the elongation of 3 times higher than that of cylinder type. In cartilage type scaffold, gelatin hydrogel printed on the outside of the scaffold was remain 22.2% under the condition where no hydrogel was left in other type scaffolds. In addition, after 2 days of breathing test, the amount of gelatin remaining inside the scaffold was more than twice that of other scaffolds. This novel trachea scaffold with hydrogel inside and outside of the structure was well-preserved under external flow and is expected to be advantageous for soft tissue reconstruction of the trachea.

scholarly journals 3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 287
Author(s):  
Ye Lin Park ◽  
Kiwon Park ◽  
Jae Min Cha
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Healing ◽  
Critical Size ◽  
Current Knowledge ◽  
3D Bioprinting ◽  
The Past ◽  
Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

3D bioprinting for lung and tracheal tissue engineering: Criteria, advances, challenges, and future directions

Bioprinting ◽  
2021 ◽  
Vol 21 ◽  
pp. e00124
Author(s):  
Seyed Hossein Mahfouzi ◽  
Seyed Hamid Safiabadi Tali ◽  
Ghassem Amoabediny
Keyword(s):  
Tissue Engineering ◽  
3D Bioprinting ◽  
Future Directions ◽  
Tracheal Tissue

Metformin-loaded nanospheres laden photocrosslinkable gelatin hydrogel for bone tissue engineering

2020 ◽  
pp. 104293
Author(s):  
Liu Qu ◽  
Nileshkumar Dubey ◽  
Juliana S. Ribeiro ◽  
Ester A.F. Bordini ◽  
Jessica A. Ferreira ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Gelatin Hydrogel

Key advances of carboxymethyl cellulose in tissue engineering & 3D bioprinting applications

2021 ◽  
Vol 256 ◽  
pp. 117561
Author(s):  
Allen Zennifer ◽  
Praseetha Senthilvelan ◽  
Swaminathan Sethuraman ◽  
Dhakshinamoorthy Sundaramurthi
Keyword(s):  
Tissue Engineering ◽  
Carboxymethyl Cellulose ◽  
3D Bioprinting

Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering

2010 ◽  
Vol 38 (6) ◽  
pp. 1977-1988 ◽  
Author(s):  
Yoshikazu Kumashiro ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Temperature Responsive
Sign in / Sign up

Export Citation Format

Share Document