scholarly journals Development of Gelatin-Coated Microspheres for Novel Bioink Design

Polymers ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 3339
Author(s):  
Muskan Kanungo ◽  
Yale Wang ◽  
Noah Hutchinson ◽  
Emma Kroll ◽  
Anna DeBruine ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Flow Rate ◽  
Bioactive Molecules ◽  
Great Promise ◽  
Charged Surface ◽  
Design Expert ◽  
Gelatin Concentration ◽  
Gelatin Coating ◽  
Positively Charged

A major challenge in tissue engineering is the formation of vasculature in tissue and organs. Recent studies have shown that positively charged microspheres promote vascularization, while also supporting the controlled release of bioactive molecules. This study investigated the development of gelatin-coated pectin microspheres for incorporation into a novel bioink. Electrospray was used to produce the microspheres. The process was optimized using Design-Expert® software. Microspheres underwent gelatin coating and EDC catalysis modifications. The results showed that the concentration of pectin solution impacted roundness and uniformity primarily, while flow rate affected size most significantly. The optimal gelatin concentration for microsphere coating was determined to be 0.75%, and gelatin coating led to a positively charged surface. When incorporated into bioink, the microspheres did not significantly alter viscosity, and they distributed evenly in bioink. These microspheres show great promise for incorporation into bioink for tissue engineering applications.

Gelatin Coated 45S5 Bioglass®-Derived Scaffolds for Bone Tissue Engineering

2013 ◽  
Vol 541 ◽  
pp. 31-39 ◽  
Author(s):  
Anke Lisa Metze ◽  
Alexandra Grimm ◽  
Patcharakamon Nooeaid ◽  
Judith A. Roether ◽  
Jasmin Hum ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Compressive Strength ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Gelatin Layer ◽  
Gelatin Concentration ◽  
Gelatin Coating ◽  
45S5 Bioglass ◽  
Highly Porous

Highly porous 45S5 Bioglass® scaffolds were fabricated by the foam replica method and successfully coated with a well attached gelatin layer by dipping and pipetting methods. Depending on macropore size of the scaffold and gelatin concentration, mechanically enhanced scaffolds with improved compressive strength in comparison to uncoated scaffolds could be obtained while preserving the high and interconnected porosity that is required for bone in-growth. Moreover, the scaffolds bioactivity by immersion in simulated body fluid (SBF) was investigated showing that gelatin coating preserves the intrinsic bioactivity of the Bioglass® scaffold. It was also shown that the gelatin layer can be loaded with tetracycline hydrochloride for developing scaffolds with drug delivery capability.

scholarly journals Investigation on the printability of bioink based on alginate-gelatin hydrogel and liquid crystals

2020 ◽  
Vol 9 (4) ◽  
pp. 1718-1725
Author(s):  
Alyaa Idrees Abdulmaged ◽  
Chin Fhong Soon ◽  
Balkis A. Talip ◽  
Sheril Amira Othman ◽  
Gim Pao Lim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Liquid Crystals ◽  
Flow Rate ◽  
Potential Application ◽  
Cell Attachment ◽  
3D Bioprinting ◽  
Minimum Width ◽  
Gelatin Hydrogel ◽  
Support Cell ◽  
Gelatin Concentration

Bioinks of 3D bioprinting have significant potential application in the field of tissue engineering to support cell attachment and proliferation. In this work, the alginate-gelatin-CELC (AGLC) bioink based on different compositions of alginate-gelatin (AG) hydrogel and cholesteryl ester liquid crystals (CELC) was prepared. Primarily, the alginate-gelatin hydrogel with certain concentration of Gelatin (10-50%w/v) was investigated. The printability of the hydrogel reached a minimum width of 1.8 mm at a flow rate of 1 mL/min when the Gelatin concentration was increased to 50 % w/v (AG1050). Subsequently, the respective polymers with 10% w/v Alginate and50% w/v Gelatin blended with 1%, 5%, 10%, 20%, 40%, and 60% w/v of CELC in the preparation of the alginate-gelatin-CELC bioink was further investigated. The printability of the bioink was examined by micro-extrusion based 3D bioprinter. The printability of the bioink enhanced by 27.8% as compared to AG1050 and reached a minimum width of 1.3 mm at a flow rate of 1 mL/min when the CELC concentration was increased to 40% and 60%. The tested properties of the bioink show that the CELC improve shear-thinning and lipid moieties properties to the composite bioink and hence, enhances its printability.

Antibiotic-anionic Clay Matrix Used for Drug Controlled Release

2018 ◽  
Vol 69 (2) ◽  
pp. 321-323
Author(s):  
Georgeta Zegan ◽  
Elena Mihaela Carausu ◽  
Loredana Golovcencu ◽  
Alina Sodor Botezatu ◽  
Eduard Radu Cernei ◽  
...  
Keyword(s):  
Controlled Release ◽  
Layered Double Hydroxides ◽  
Bioactive Molecules ◽  
Drug Controlled Release ◽  
Anionic Clays ◽  
Anionic Clay ◽  
Toxicological Studies ◽  
High Efficient ◽  
Clay Matrix ◽  
Double Hydroxides

Anionic clay matrix acting as drug controlled release system have shown in last years a great potential for delivery of bioactive molecules and chemical therapeutics. This organic-inorganic nanohybrid system is high efficient offering an excellent protection of intercalated compounds from degradation. Compared to other nanoparticles used in medical area, anionic clays type layered double hydroxides have found to be biocompatible according to toxicological studies. Ampicillin containing MgAlLDHs and ZnAlLDH samples have been prepared following two routes: anion-exchange procedure and reconstruction from calcined layered double hydroxides. Solid samples have been characterized by FTIR and SEM-EDX highlighting the alteration of pristine LDHs structure when the antibiotic is introduced in the interlayer gallery.

Polysaccharide-Based Nanobiomaterials as Controlled Release Systems for Tissue Engineering Applications

2015 ◽  
Vol 21 (33) ◽  
pp. 4837-4850 ◽  
Author(s):  
Eustolia Rodriguez-Velazquez ◽  
Manuel Alatorre-Meda ◽  
Joao F. Mano
Keyword(s):  
Tissue Engineering ◽  
Controlled Release ◽  
Engineering Applications

scholarly journals Gum Tragacanth (GT): A Versatile Biocompatible Material beyond Borders

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1510 ◽  
Author(s):  
Mohammad Ehsan Taghavizadeh Yazdi ◽  
Simin Nazarnezhad ◽  
Seyed Hadi Mousavi ◽  
Mohammad Sadegh Amiri ◽  
Majid Darroudi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Food Packaging ◽  
Three Dimensional ◽  
Great Promise ◽  
Anionic Polymer ◽  
Gum Tragacanth ◽  
New Horizons ◽  
Tissue Healing ◽  
The Stability ◽  
Therapeutic Substance

The use of naturally occurring materials in biomedicine has been increasingly attracting the researchers’ interest and, in this regard, gum tragacanth (GT) is recently showing great promise as a therapeutic substance in tissue engineering and regenerative medicine. As a polysaccharide, GT can be easily extracted from the stems and branches of various species of Astragalus. This anionic polymer is known to be a biodegradable, non-allergenic, non-toxic, and non-carcinogenic material. The stability against microbial, heat and acid degradation has made GT an attractive material not only in industrial settings (e.g., food packaging) but also in biomedical approaches (e.g., drug delivery). Over time, GT has been shown to be a useful reagent in the formation and stabilization of metal nanoparticles in the context of green chemistry. With the advent of tissue engineering, GT has also been utilized for the fabrication of three-dimensional (3D) scaffolds applied for both hard and soft tissue healing strategies. However, more research is needed for defining GT applicability in the future of biomedical engineering. On this object, the present review aims to provide a state-of-the-art overview of GT in biomedicine and tries to open new horizons in the field based on its inherent characteristics.

scholarly journals Molecular Imprinting Strategies for Tissue Engineering Applications: A Review

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 548
Author(s):  
Amedeo Franco Bonatti ◽  
Carmelo De Maria ◽  
Giovanni Vozzi
Keyword(s):  
Tissue Engineering ◽  
Molecularly Imprinted Polymers ◽  
Cell Interaction ◽  
Great Promise ◽  
Molecularly Imprinted ◽  
Proliferation And Differentiation ◽  
Promising Solution ◽  
Natural Tissue ◽  
A Current ◽  
Biophysical Cues

Tissue Engineering (TE) represents a promising solution to fabricate engineered constructs able to restore tissue damage after implantation. In the classic TE approach, biomaterials are used alongside growth factors to create a scaffolding structure that supports cells during the construct maturation. A current challenge in TE is the creation of engineered constructs able to mimic the complex microenvironment found in the natural tissue, so as to promote and guide cell migration, proliferation, and differentiation. In this context, the introduction inside the scaffold of molecularly imprinted polymers (MIPs)—synthetic receptors able to reversibly bind to biomolecules—holds great promise to enhance the scaffold-cell interaction. In this review, we analyze the main strategies that have been used for MIP design and fabrication with a particular focus on biomedical research. Furthermore, to highlight the potential of MIPs for scaffold-based TE, we present recent examples on how MIPs have been used in TE to introduce biophysical cues as well as for drug delivery and sequestering.

Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Cross Linking ◽  
Layer By Layer ◽  
Multilayer Composite ◽  
Composite Beads ◽  
Assembly Technique ◽  
Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

scholarly journals Polymeric Bioinks for 3D Hepatic Printing

Chemistry ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 164-181
Author(s):  
Joyita Sarkar ◽  
Swapnil C. Kamble ◽  
Nilambari C. Kashikar
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Synthetic Polymers ◽  
Bioactive Molecules ◽  
Complex Geometries ◽  
Printing Techniques ◽  
Natural And Synthetic

Three-dimensional (3D) printing techniques have revolutionized the field of tissue engineering. This is especially favorable to construct intricate tissues such as liver, as 3D printing allows for the precise delivery of biomaterials, cells and bioactive molecules in complex geometries. Bioinks made of polymers, of both natural and synthetic origin, have been very beneficial to printing soft tissues such as liver. Using polymeric bioinks, 3D hepatic structures are printed with or without cells and biomolecules, and have been used for different tissue engineering applications. In this review, with the introduction to basic 3D printing techniques, we discuss different natural and synthetic polymers including decellularized matrices that have been employed for the 3D bioprinting of hepatic structures. Finally, we focus on recent advances in polymeric bioinks for 3D hepatic printing and their applications. The studies indicate that much work has been devoted to improvising the design, stability and longevity of the printed structures. Others focus on the printing of tissue engineered hepatic structures for applications in drug screening, regenerative medicine and disease models. More attention must now be diverted to developing personalized structures and stem cell differentiation to hepatic lineage.

Synthesis, characterization and drug release studies of poly(2-hydroxyethyl methacrylate)/KIT-5 nanocomposite as an innovative organic–inorganic hybrid carrier system

RSC Advances ◽  
2015 ◽  
Vol 5 (16) ◽  
pp. 12463-12471 ◽  
Author(s):  
Roozbeh Javad Kalbasi ◽  
Ali Zirakbash
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Hybrid Material ◽  
Great Promise ◽  
Hydroxyethyl Methacrylate ◽  
Carrier System ◽  
Inorganic Hybrid ◽  
Drug Molecules ◽  
System P ◽  
Release Studies

PHEMA/KIT-5 with various pore sizes was prepared. Efficient encapsulation of drug molecules inside the pores of the hybrid material and controlled release of them in an aqueous medium, suggest the great promise of the composite as a carrier system.

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